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    Korean J Urol. 2006 Feb;47(2):201-205. Korean.
    Published online Feb 28, 2006.
    https://doi.org/10.4111/kju.2006.47.2.201
    Copyright © 2006 The Korean Urological Association
    Original Article

    Expression Pattern of Neuroendocrine Cells and Survivin in the Prostate of Rabbits

    Jun Mo Kim, Kwang Woo Lee, Young Ho Kim, Eun Suk Go,1 Min Eui Kim and Nam Kyu Lee
      • Department of Urology, Soonchunhyang University College of Medicine, Bucheon, Korea.
      • 1Department of Pathology, Soonchunhyang University College of Medicine, Bucheon, Korea.
    • Corresponding author (Email: Beetho@schch.co.kr )
    Received June 13, 2005; Accepted August 04, 2005.

    Abstract

    Purpose

    The neuroendocrine cell (NE cell) is thought to play an important role in the development of hormone-refractory prostate cancer. Survivin is one of the IAPs (inhibitors of apoptosis), and it is expressed in the NE cell and in most of the common cancers, but not in normal tissue. The objective of this study was to investigate the expression pattern of the NE cell and survivin in the prostate of rabbits.

    Materials and Methods

    The 9 rabbits underwent orchiectomy and their prostates were removed at 0 weeks (control), 2 weeks and 6 weeks after orchiectomy. Each of the prostatic tissue specimens was stained with H&E; immunohistochemical staining was done for chromogranin A, synaptophysin and survivin, and the tissue specimens were then examined by microscopy.

    Results

    In the prostate of rabbits, most of the NE cells were located between the epithelial gland and the stroma. NE differentiation occurred 6 weeks after orchiectomy. The location of cells that were positive for survivin was almost same as that of the NE cells.

    Conclusions

    The main location of NE cells in the prostate of rabbits was between the epithelial gland and the stroma, and NE differentiation occurred 6 weeks after orchiectomy, the same as in a human or a dog. The location of survivin positive cells coincided with that of the NE cells. Therefore, a rabbit seems to be a suitable animal model for the study of the NE cell.

    Keywords
    APUD cells; Survivin; Prostate; Orchiectomy; Rabbits

    Figures

    Fig. 1
    Hematoxylin Eosin stain (A-C) and immunohistochemical stain with monoclonal antibodies to CgA (D-F), SNP (G-I) and survivin (J-L) in the prostate of rabbits before orchiectomy (A, D, G, J), 2 weeks after (B, E, H, K), and 6 weeks after orchiectomy (C, F, I, L) (×100: A-C) (×400: D-L). In the prostate of rabbits, the shape of the acini became shrunken and the number of epithelial cells are decreased after orchiectomy. Although most NE cells are located between the stroma and the glandular epithelium, there is the small number of NE cells in the glandular epithelium, as noted on the SNP staining. NE differentiation is found 6 weeks after of orchectomy. Also, the cells that have a positive reaction to survivin are found in all of the three groups. The location of the cells positive to survivin is compatible with that of the NE cell, and the number of cells is increased after 4 weeks.

    Tables

    Table 1
    Gross and microscopic findings of the rabbit prostate

    References

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    MeSH Terms
    APUD Cells
    Animals
    Chromogranin A
    Dogs
    Humans
    Microscopy
    Models, Animal
    Neuroendocrine Cells*
    Orchiectomy
    Prostate*
    Prostatic Neoplasms
    Rabbits*
    Synaptophysin
    Metrics
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