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    Korean J Urol. 2006 Oct;47(10):1111-1116. Korean.
    Published online Oct 31, 2006.
    https://doi.org/10.4111/kju.2006.47.10.1111
    Copyright © 2006 The Korean Urological Association
    Original Article

    The Suppressive Effect of Bovine Colostrum Combined with Antibiotics on Renal Inflammation

    Jong Kyu Park, Heung Jae Park and Kwan Joong Joo
      • Department of Urology, Sungkyunkwan University School of Medicine, Seoul, Korea.
    • Corresponding author (Email: urojoo@dreamwiz.com )
    Received July 03, 2006; Accepted August 10, 2006.

    Abstract

    Purpose

    Bovine colostrum contains three times more immunoglobulin than human colostrums does. We investigated the effectiveness of administering Bovine colostrum combined with antibiotics in an animal model of ascending pyelonephritis.

    Materials and Methods

    Thirty female Sprague-Dawley rats were divided into 5 groups: the normal group, the non-treated control group, the Bovine colostrum administered group, the antibiotic treatment group and the combined administration of antibiotics and Bovine colostrums group. After anesthetizing, an inoculum of 1×108 colony forming units per ml of Escherichia coli (E. coli, ATCC 25922 strain) was instilled into the bladder through the urethra and the urethra was occluded for 4 hours. We began treatment with ciprofloxacin (15mg/kg, for 5 days, intramuscularly) alone or Bovine colostrum (6ml/kg, for 5 days, orally) alone or with combination treatment 72 hours after inoculation. The rats were sacrificed 4 weeks after infection. Both kidneys were examined pathologically. We carried bacterial culture examinations of the urine and the kidney tissue.

    Results

    As the results of examining the cultures of the urine and kidney tissues, no bacteria were cultivated in the antibiotics treatment group and the combined treatment group. 66.7% of the control group and 33.3% of the bovine colostrum treatment group had positive urine cultures and 66.7% of the control group and 41.7% of the bovine colostrum treatment group showed a positive reaction on the kidney tissue culture. The severity of pyelonephritis, as noted on the pathological examination, was highest in the control group. The combined treatment group had the lowest degree of infection among all the groups.

    Conclusions

    The administration of bovine colostrum in combination with antibiotics can significantly reduce the inflammation associated with ascending pyelonephritis.

    Keywords
    Colostrum; Pyelonephritis; Inflammation

    Figures

    Fig. 1
    Grade of inflammation of each group. The degree of inflammation is lowest for the combined treatment group.

    Fig. 2
    Light microscopic findings of the renal prenchyma 4 weeks after infection. The control group (A) and the bovine colostrum treatment group (B). Marked inflammation of the renal parenchyma is seen at both group. Yet the inflammation of the bovine colostrum treatment group is less severe than that of the bovine colostrum treatment group (H&E, ×100).

    Fig. 3
    Light microscopic findings of the renal prenchyma 4 weeks after infection. The antibiotic treatment group (A) and the combined treatment group (B). The area of inflammation of the combined treatment group is much smaller than that of the antibiotic treatment group (H&E, ×100).

    Tables

    Table 1
    Weight of the kidney and the number of positive urine and kidney cultures

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    MeSH Terms
    Animals
    Anti-Bacterial Agents*
    Bacteria
    Ciprofloxacin
    Colostrum*
    Escherichia coli
    Female
    Humans
    Immunoglobulins
    Inflammation*
    Kidney
    Models, Animal
    Pyelonephritis
    Rats
    Rats, Sprague-Dawley
    Stem Cells
    Urethra
    Urinary Bladder
    Metrics
    Share
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