A Combination of PG490 and Lipopolysaccharide Induce Apoptosis through Activation of Casapase-3 and Down-regulation of cIAP1 and XIAP in Human Astroglioma Cell

Lee, Tae-Jin; Woo, Kyung-Jin; Park, Jong-Wook; Kwon, Taeg Kyu

doi:10.4110/in.2005.5.2.99

Immune Netw. 2005 Jun;5(2):99-104. English.
Published online Jun 30, 2005.
https://doi.org/10.4110/in.2005.5.2.99

Original Article

A Combination of PG490 and Lipopolysaccharide Induce Apoptosis through Activation of Casapase-3 and Down-regulation of cIAP1 and XIAP in Human Astroglioma Cell

Tae-Jin Lee, Kyung-Jin Woo, Jong-Wook Park and Taeg Kyu Kwon

Author information

Author notes

Copyright and License

- Department of Immunology and Chronic Disease Research Center and Institute for Medical Science, School of Medicine, Keimyung University, Daegu, South Korea.
Correspondence to: Taeg Kyu Kwon, Department of Immunology, School of Medicine, Keimyung University, 194 Dongsan-dong, Jung-gu, Daegu 700-712, South Korea. (Tel) +82-53-250-7846, (Fax) +82-53-250-7074, Email: kwontk@dsmc.or.kr

This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Malignant gliomas are the most common primary tumors in the central nervous system.

Methods

We investigated the combined effect of PG490 and LPS on the induction of the apoptotic pathway in human astroglioma cells.

Results

Treatment of U87 cells with combination of 50 nM of PG490 and 50µg/ml of LPS resulted in increased internucleosomal DNA fragmentation, cleavage of PLC-γ1, and downregulation of cIAP1 and XIAP. The combination of LPS and PG490 treatment-induced apoptosis is mediated through the activation of caspase, which is inhibited by the caspase inhibitor, z-VAD-fmk. Also, release of cytochrome c was found in PG490 and LPS-cotreated U87 cell.

Conclusion

Taken together, combination of PG490 and LPS appears to be a potent inducer of apoptosis in astrogliaoma cells, and might have some benefit in the treatment of glioma patients.

Keywords

LPS; PG490; apoptosis; astroglioma