Published online Jun 30, 2005.
https://doi.org/10.4110/in.2005.5.2.105
The Expression of Galectin-3, a Beta-Galactoside Binding Protein, in Dendritic Cells
Abstract
Background
Dendritic cells (DCs) are the most potent APCs (antigen-presenting cells) and play a critical role in immune responses. Galectin-3 is a biological lectin with a beta-galactoside binding affinity. Recently, proteomic analysis revealed the presence of galectin-3 in the exosome of mature DCs. However, the expression and function of galectin-3 in DCs remains unclear yet.
Methods
We used bone marrow-derived DCs of mouse and showed the expression of galectin-3 in DCs by using flow cytometry analysis and Western blot analysis.
Results
Galectin-3 was determined as single band of 35 kDa in Western blot analysis. Flow cytometry analysis showed the major growth factor for DCs, granulocyte-macrophage colony stimulating factor (GM-CSF) and maturing agents, anti-CD40 monoclonal antibody (mAb) and lipopolysaccharide (LPS) consistently increased the intracellular expression of galectin-3 in DCs compared to medium alone. In addition, DCs treated with maturing agents did marginally express galectin-3 on their surface.
Conclusion
This study suggests that galectin-3 in DCs may be regulated by critical factors for DC function.
