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Effect of insulin-like growth factor-1 and hyaluronic acid in experimentally produced osteochondral defects in rats

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Abstract

Background

The common purpose of almost all methods used to treat the osteochondral injuries is to produce a normal cartilage matrix. However current methods are not suffcient to provide a normal cartilage matrix. For that reason, researchers have studied to increase the effectiveness of this methods using chondrogenic and chondroprotective molecules in recent experimental studies. Insulin-like growth factor-1 (IGF-1) and hyaluronic acid (HA) are two important agents used in this feld. This study compared the effects of IGF-1 and HA in an experimental osteochondral defect in rat femora.

Materials and Methods

The rats were divided into three groups (n = 15 per group) as follows: The IGF-1 group, HA group, and control group. An osteochondral defect of a diameter of 1.5 mm and a depth of 2 mm was created on the patellar joint side of femoral condyles. The IGF-1 group received an absorbable gelatin sponge soaked with 15 μg/15 μl of IGF-1, and the HA group received an absorbable gelatin sponge soaked with 80 μg HA. The control group received only an absorbable gelatin sponge. Rats were sacrifced at the 6th week, and the femur condyles were evaluated histologically.

Results

According to the total Mankin scale, there was a statistically signifcant difference between IGF-1 and HA groups and between IGF-1 and control groups. There was also a signifcant statistical difference between HA and control groups.

Conclusion

It was shown histopathologically that IGF-1 is an effective molecule for osteochondral lesions. Although it is weaker than IGF-1, HA also strengthened the repair tissue.

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Authors and Affiliations

  1. Department of Orthopaedics, Düzce University Medical Faculty, Düzce, Turkey

    Istemi Yücel, Ramazan Atiç & Emin Özkul

  2. Department of Orthopaedics, Tekirdag State Hospital, Tekirdag, Turkey

    Baris Erbil

  3. Department of Pathology, Ordu University Medical Faculty, Ordu, Turkey

    Havva Erdem

  4. Department of Orthopaedics, Dicle University Medical Faculty, Diyarbakir, 21280, Turkey

    Celil Alemdar

Authors
  1. Celil Alemdar
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  2. Istemi Yücel
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  3. Baris Erbil
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  5. Ramazan Atiç
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Correspondence to Celil Alemdar.

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Alemdar, C., Yücel, I., Erbil, B. et al. Effect of insulin-like growth factor-1 and hyaluronic acid in experimentally produced osteochondral defects in rats. IJOO 50, 414–420 (2016). https://doi.org/10.4103/0019-5413.185607

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  • DOI: https://doi.org/10.4103/0019-5413.185607

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