https://doi.org/10.4081/ejh.2023.3577

The role of miR-143-3p/FNDC1 axis on the progression of non-small cell lung cancer

Vol. 67 No. 2 (2023)
Submitted: 12 October 2022
Accepted: 22 March 2023
Published: 3 May 2023
FNDC1, miR-143-3p, non-small cell lung cancer (NSCLC)
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Authors

Zhanshu Ma
Department of Radiotherapy, Affiliated Hospital of Chifeng University, Chifeng, China.
Qi Gao
Department of Radiotherapy, Affiliated Hospital of Chifeng University, Chifeng, China.
Wenjing Xin
Department of Radiotherapy, Affiliated Hospital of Chifeng University, Chifeng, China.
Lei Wang
Department of Radiotherapy, Affiliated Hospital of Chifeng University, Chifeng, China.
Yan Chen
Department of Radiotherapy, Affiliated Hospital of Chifeng University, Chifeng, China.
Chang Su
Department of Radiotherapy, Affiliated Hospital of Chifeng University, Chifeng, China.
Songyan Gao
Department of Radiotherapy, Affiliated Hospital of Chifeng University, Chifeng, China.
Ruiling Sun
gret258@163.com
Department of Radiotherapy, Affiliated Hospital of Chifeng University, Chifeng, China.

The study aimed to explore the functional role of fibronectin type III domain containing 1 (FNDC1) in nonsmall cell lung cancer (NSCLC), as well as the mechanism governing its expression. The expression levels of FNDC1 and related genes in tissue and cell samples were detected by qRT-PCR. Kaplan-Meier analysis was employed to analyze the association between FNDC1 level and the overall survival of NSCLC patients. Functional experiments such as CCK-8 proliferation, colony formation, EDU staining, migration and invasion assays were conducted to investigate the functional role of FNDC1 in regulating the malignancy of NSCLC cells. Bioinformatic tools and dual-luciferase reporter assay were used to identify the miRNA regulator of FNDC1 in NSCLC cells. Our data revealed the upregulation of FNDC1 at mRNA and protein levels in NSCLC tumor tissues cancer cell lines, compared with normal counterparts. NSCLC patients with higher FNDC1 expression suffered from a poorer overall survival. FNDC1 knockdown significantly suppressed the proliferation, migration and invasion of NSCLC cells, and had an inhibitory effect on tube formation. We further demonstrated that miR-143-3p was an upstream regulator of FNDC1 and miR-143-3p expression was repressed in NSCLC samples. Similar to FNDC1 knockdown, miR-143-3p overexpression inhibited the growth, migration and invasion of NSCLC cells. FNDC1 overexpression could partially rescue the effect of miR-143-3p overexpression.  FNDC1 silencing also suppressed the tumorigenesis of NSCLC cells in mouse model. In conclusion, FNDC1 promotes the malignant prototypes of NSCLC cells. miR-143-3p is a negative regulator of FNDC1 in NSCLC cells, which may serve as a promising therapeutic target in NSCLC. 

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Citations

Crossref
Scopus
Google Scholar
Europe PMC
Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin 2018;68:394-424. DOI: https://doi.org/10.3322/caac.21492
Zappa C, Mousa SA. Non-small cell lung cancer: Current treatment and future advances. Transl Lung Cancer Res 2016;5:288-300. DOI: https://doi.org/10.21037/tlcr.2016.06.07
Dias M, Linhas R, Campainha S, Conde S, Barroso A. Lung cancer in never-smokers–what are the differences? Acta Oncologica 2017;56:931-5. DOI: https://doi.org/10.1080/0284186X.2017.1287944
Sun S, Schiller JH, Gazdar AF. Lung cancer in never smokers - A different disease. Nat Rev Cancer 2007;7:778-90. DOI: https://doi.org/10.1038/nrc2190
Lemjabbar-Alaoui H, Hassan OUI, Yang YW, Buchanan P. Lung cancer: biology and treatment options. Biochim Biophys Acta Rev Cancer 2015;1856:189-210. DOI: https://doi.org/10.1016/j.bbcan.2015.08.002
Schabath MB, Cote ML. Cancer progress and priorities: lung cancer. Cancer Epidemiol Biomarkers Prev 2019;28:1563-79. DOI: https://doi.org/10.1158/1055-9965.EPI-19-0221
Raso MG, Behrens C, Herynk MH, Liu S, Prudkin L, Ozburn NC, et al. Immunohistochemical expression of estrogen and progesterone receptors identifies a subset of NSCLCs and correlates with EGFR mutation. Clin Cancer Res 2009;15:5359-68. DOI: https://doi.org/10.1158/1078-0432.CCR-09-0033
Wang DC, Wang W, Zhu B, Wang X. Lung cancer heterogeneity and new strategies for drug therapy. Annu Rev Pharmacol Toxicol 2018;58:531-46. DOI: https://doi.org/10.1146/annurev-pharmtox-010716-104523
Lin TC, Yang CH, Cheng LH, Chang WT, Lin YR, Cheng HC. Fibronectin in cancer: friend or foe. Cells 2020;9:27. DOI: https://doi.org/10.3390/cells9010027
Pankov R, Yamada KM. Fibronectin at a glance. J Cell Sci 2002;115:3861-3. DOI: https://doi.org/10.1242/jcs.00059
Albrecht M, Renneberg H, Möschler O, Janssen M, Aumüller G, Konrad L, et al. Fibronectin in human prostatic cells in vivo and in vitro: expression, distribution, and pathological significance. Histochem Cell Biol 1999;112:51-61. DOI: https://doi.org/10.1007/s004180050391
Fernandez-Garcia B, Eiró N, Marín L, González-Reyes S, González LO, Lamelas ML, et al. Expression and prognostic significance of fibronectin and matrix metalloproteases in breast cancer metastasis. Histopathology 2014;64:512-22. DOI: https://doi.org/10.1111/his.12300
Anderegg U, Breitschwerdt K, Köhler MJ, Sticherling M, Haustein UF, Simon JC, et al. MEL4B3, a novel mRNA is induced in skin tumors and regulated by TGF-β and pro-inflammatory cytokines. Exp Dermatol 2005;14:709-18. DOI: https://doi.org/10.1111/j.0906-6705.2005.00349.x
Das DK, Naidoo M, Ilboudo A, Park JY, Ali T, Krampis K, et al. miR-1207-3p regulates the androgen receptor in prostate cancer via FNDC1/fibronectin. Exp Cell Res 2016;348:190-200. DOI: https://doi.org/10.1016/j.yexcr.2016.09.021
Jiang T, Gao W, Lin S, Chen H, Du B, Liu Q, et al. FNDC1 Promotes the invasiveness of gastric cancer via Wnt/β-catenin signaling pathway and correlates with peritoneal metastasis and prognosis. Front Oncol 2020;10:590492. DOI: https://doi.org/10.3389/fonc.2020.590492
Menon SS, Guruvayoorappan C, Sakthivel KM, Rasmi RR. Ki-67 protein as a tumour proliferation marker. Clin Chim Acta 2019;491:39-45. DOI: https://doi.org/10.1016/j.cca.2019.01.011
Liu YP, Chen WD, Li WN, Zhang M. Overexpression of FNDC1 relates to poor prognosis and its knockdown impairs cell invasion and migration in gastric cancer. Technol Cancer Res Treat 2019;18:153303381986992. DOI: https://doi.org/10.1177/1533033819869928
Taniguchi K, Sugito N, Kumazaki M, Shinohara H, Yamada N, Nakagawa Y, et al. MicroRNA-124 inhibits cancer cell growth through PTB1/PKM1/PKM2 feedback cascade in colorectal cancer. Cancer Letters 2015;363:17-27. DOI: https://doi.org/10.1016/j.canlet.2015.03.026
Bell A, Bell D, Weber RS, El-Naggar AK. CpG island methylation profiling in human salivary gland adenoid cystic carcinoma. Cancer 2011;117:2898-909. DOI: https://doi.org/10.1002/cncr.25818
Di Leva G, Garofalo M, Croce CM. MicroRNAs in cancer. Annu Rev Pathol 2014;9:287-314. DOI: https://doi.org/10.1146/annurev-pathol-012513-104715
Lin T, Dong W, Huang J, Pan Q, Fan X, Zhang C, et al. MicroRNA-143 as a tumor suppressor for bladder cancer. J Urol 2009;181:1372-80. DOI: https://doi.org/10.1016/j.juro.2008.10.149
Liu L, Yu X, Guo X, Tian Z, Su M, Long Y, et al. MiR-143 is downregulated in cervical cancer and promotes apoptosis and inhibits tumor formation by targeting Bcl-2. Mol Med Rep 2012;5:753-60.
Wu XL, Cheng B, Li PY, Huang HJ, Zhao Q, Dan ZL, et al. MicroRNA-143 suppresses gastric cancer cell growth and induces apoptosis by targeting COX-2. World J Gastroenterol 2013;19:7758-65. DOI: https://doi.org/10.3748/wjg.v19.i43.7758

Ethics Approval

the study was approved by the Ethics Committee Board of Affiliated Hospital of Chifeng University, All animal procedures were approved by the Animal Care and Use Ethical Committee of Affiliated Hospital of Chifeng University

How to Cite

Ma, Z., Gao, Q., Xin, W., Wang, L., Chen, Y., Su, C., Gao, S., & Sun, R. (2023). The role of miR-143-3p/FNDC1 axis on the progression of non-small cell lung cancer. European Journal of Histochemistry, 67(2). https://doi.org/10.4081/ejh.2023.3577
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