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A TECHNIQUE FOR MEASURING AND MARKING LIVE FLIES

Published online by Cambridge University Press:  31 May 2012

Russell Bonduriansky
Affiliation:
Department of Zoology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Ronald J. Brooks
Affiliation:
Department of Zoology, University of Guelph, Guelph, Ontario, Canada N1G 2W1

Abstract

We designed a simple device for physically restraining live Diptera for measurement and marking. This new technique does not involve powders, anaesthetics, or handling with fingers or forceps, and can be adapted for use with flies of any size. We tested it on acalyptrate flies of 1.5–3.0 mm body length, and were able to sex, measure, and mark them with individual codes (enamel paint applied to the thoracic notum) in 5–7 min per fly, with a 96% success rate. This technique may be applicable to a range of behavioural and ecological research.

Résumé

Nous avons mis au point une technique simple destinée à immobiliser physiquement les diptères vivants pour les mesurer et les marquer. Cette nouvelle technique n’utilise aucune poudre ou substance anesthésiante et ne suppose aucune manipulation avec les mains ou avec des pinces; elle peut être adaptée à des mouches de toutes les tailles. La technique a été testée sur des acalyptères de longueur totale de 1,5 à 3,0 mm et nous avons pu déterminer le sexe chez chacune des mouches, les mesurer et les marquer de codes individuels (touche d’émail sur le notum thoracique), tout cela en. 5–7 minutes par mouche, avec un taux de succès de 96%. Cette technique est utilisable dans une grande variété de types de recherche comportementale et écologique.

[Traduit par la Rédaction]

Type
Articles
Copyright
Copyright © Entomological Society of Canada 1997

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