Short Communications
DNA Typing of Dessert Banana Cultivar 'Kolikuttu' ('Silk') Accessions by Microsatellite Markers
Authors:
- HWL PushpakumariEmail HWL Pushpakumari
- WLG Samarasinghe
- SGJN Senanayake
Abstract
Fourteen Kolikuttu accessions showing morphological variation and Fusarium wilt tolerance were collected. Extracted DNA was subjected to PCR using six SSR primers. PCR products were separated by PAGE and bands were visualized, scored and statistically analyzed using POPGENE 1.32 version to obtain the dendrogram by genetic distance matrix. Out of 19 SSR alleles amplified, 12 were polymorphic resulting in 63.16% loci polymorphism. Based on the dendrogram, three different "Kolikuttu" accessions known as "Athdath Puwalu", the accession collected from Telijjawila, and accession collected from Angunakolapelassa were clustered separately. These three accessions could be considered as different genotypes.Key words: DNA Typing; Dessert Banana; Cultivar Kolikuttu
DOI: 10.4038/tare.v12i2.2801
Tropical Agricultural Research & Extension 12(2): 2009 pp.113-116
- Year: 2009
- Volume: 12 Issue: 2
- Page/Article: 113-116
- DOI: 10.4038/tare.v12i2.2801
- Published on 1 Feb 2011
- Peer Reviewed