Building Functional Surfaces for Biosensors Development

Carlos Morón; Alfonso Garcia; Enrique Tremps; Jose Andrés Somolinos

doi:10.4028/www.scientific.net/KEM.543.204

Paper Titles

Synthesis of Eu-Doped TiO₂ Nanotubes Using Electrochemical Oxidation
p.188

Dodecane Sensing with Double Substituted LaCoO₃ Nanowires Deposited by Reactive Magnetron Sputtering
p.192

Electrical Properties and SEM Study of Foamed EBA/Carbon Black Composites
p.196

Biosafety Evaluation of Nanoparticles in View of Genotoxicity and Carcinogenicity Studies: A Systematic Review
p.200

Building Functional Surfaces for Biosensors Development
p.204

Nanostructured Membranes for MEMS Energy Harvester
p.208

Mechanical, Electrical and Thermal Characterization of Commercially Available LTCC Dielectric Tapes
p.212

Measurement of Putting Golf Balls Motion Using a Vision Sensor
p.216

Numerical Investigation of Propagation of Ultrasonic Waves in the Waveguides with Mode Conversion
p.219

HomeKey Engineering MaterialsKey Engineering Materials Vol. 543Building Functional Surfaces for Biosensors...

Building Functional Surfaces for Biosensors Development

Abstract:

Polyelectrolyte multilayers (PEM) built by layer-by-layer technique have been extensively studied over the last years, resulting in a wide variety of current and potential applications. This technique can be used to construct thin films with different functionalities, or to functionalize surfaces with substantial different properties of those of the underlying substrates. The multilayering process is achieved by the alternate adsorption of oppositely charged polyelectrolytes. In this work we get advantage of the protein resistant property of the Poly (l-lysine)-graft-(polyethyleneglycol) to create protein patterns. Proteins can be immobilized on a surface by unspecific physical adsorption, covalent binding or through specific interactions. The first protein used in this work was laccase, a copper-containing redox enzyme that catalyse the oxidation of a broad range of polyphenols and aromatic substrates, coupled to the reduction of O₂ to H₂O without need of cofactors. Applications of laccases have been reported in food, pulp, paper, and textile industry, and also in biosensor development. Some uses require the immobilization of the enzyme on solid supports by adsorption, covalent attachment, entrapment, etc, on several substrates. Especially for biosensor development, highly active, stable and reproducible immobilization of laccase is required.