Efficient Expression and Purification of Bombina maxima Trefoil Factors 2 Variant in Escherichia coli as Fusion Protein

Tong Yi Sun

doi:10.4028/www.scientific.net/AMR.926-930.1187

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 926-930Efficient Expression and Purification of Bombina...

Efficient Expression and Purification of Bombina maxima Trefoil Factors 2 Variant in Escherichia coli as Fusion Protein

Abstract:

Bm-TFF2 was isolated from Bombina maxima. The B.maxima Trefoil factors 2 Variant (vBm-TFF2) could be developed as a novel administered wound healing agent. The challenge is its preparation from refolding insoluble protein expressed in Escherichia coli. A recombinant vBm-TFF2 was overexpressed in E. coli cells as a fusion protein with bacterial N-utilizing substance A (NusA). The fusion protein NusA-Bm-TFF2 can promote the wound healing.

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Periodical:

Advanced Materials Research (Volumes 926-930)

Pages:

1187-1190

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.926-930.1187

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Online since:

May 2014

Authors:

Tong Yi Sun*

Keywords:

Bm-TFF2, Escherichia coli, Expression, Purification

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* - Corresponding Author

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