Gradient Elution Separation of Main Ingredients from Polygoni Multiflori Extract by HPLC

Tao Wang

doi:10.4028/www.scientific.net/AMR.864-867.503

Paper Titles

Research on Serum Spectrum Analysis Model Applied to Pathema Identification
p.486

The Technological Research of Diosgenin Extraction by Natural Fermentation Method
p.490

Acute Toxicity of Trimethyltin Choloride on the Sea Urchin (Strongylocentyotus intermedius) Embryos and Larvae
p.495

Airflow Drying of Corn Stalks Particles in Straight Horizontal Tube
p.499

Gradient Elution Separation of Main Ingredients from Polygoni Multiflori Extract by HPLC
p.503

Refining the Very Important Value of Mass to Charge Ratio from Traditional Chinese Medicine' Nature Data
p.508

Study the Relationship between Chemical Components of Complex Prescription and Blood Flow of the Ileus Rats
p.512

Determination of Pentachloronitrobenzene in Panax Ginseng by HPLC
p.516

Study on Extraction of Polyphenol from Grape Peel Microwave-Assisted Activity
p.520

HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 864-867Gradient Elution Separation of Main Ingredients...

Gradient Elution Separation of Main Ingredients from Polygoni Multiflori Extract by HPLC

Abstract:

In this experiment, the main compounds of stilbene glucoside and anthraquinones from polygonum multiflorum extract were separated by HPLC gradient elution. SinoChrom ODS-BP C18 is selected as chromatographic column. The optimal experimental conditions under 254nm wavelength UV detector include the column temperature: 26°C, mobile phase: methanol/water, and flow rate: 1.0ml/mol. The gradient method can be optimized by changing the steepness and shape of gradient elution. Through optimizing gradient method, the separation efficiency is improved within a certain range of gradient elution strength. This article provides important reference for quality control and content determination of fleece-flower root.

You might also be interested in these eBooks

View Preview

Info:

Periodical:

Advanced Materials Research (Volumes 864-867)

Pages:

503-507

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.864-867.503

Citation:

Cite this paper

Online since:

December 2013

Authors:

Tao Wang*

Keywords:

Anthraquinones, HPLC Gradient Elution, Polygonum Multiflorum Extract, Stilbene Glucoside

Export:

RIS, BibTeX

Price:

Permissions:

Request Permissions

* - Corresponding Author

References

[1] Su Wei, The modern pharmacological research situation of fleece-flower root[J]. Journal of Chinese herbal medicine. 1999, 28(2): 119, in Chinese.

Google Scholar

[2] Xie Wen-jie, Li Lin, Wei Hai-feng, Effect of 2, 3, 5, 4-tetrahydroxystilbene-2-O-β-D-glu-coside on hippocampal genes expression in mimetic-dementia mice induced by D-galactose[J]. Chinese Journal of Pharamacology and Toxicology. 2005, 19(1): 24-28, in Chinese.

Google Scholar

[3] Shi Guo-bing, Study on the influence of concoction over active ingredients in Polygonum multiflorum[J]. Chinese Journal of Hospital Pharmacy. 2003, 23(2): 95-97, in Chinese.

Google Scholar

[4] Nation Pharmacopoeia Committee. China Phramacopoeia. Vol. Beijing: China Medical Science Press. 2010: 164-165, in Chinese.

Google Scholar

[5] Jia Huzhan, Dong Zehong, Yu Jing, Modern research and application of Chinese medicine. Beijing: Xueyuan publishing company. 1997, Vol. 3: 2312, in Chinese.

Google Scholar

[6] Zhu Pei-fang, Zhao Rong-hua, Shi Yang-xian, Determination of stilbeneglycoside and 5 kinds of anthraquinone in Polygonum multifl orum Thunb by HPLC stimultaneously[J]. Chinese Journal of Traditional Chinese medicine. February, Vol. 27 (2012).

Google Scholar