Characterization of Tomato Fruit LeRIN Gene Application Virus-Induced Gene Silencing Technology

Ling Li; Guo Xia Zhu; Zheng Liu; Tie Ling Liu

doi:10.4028/www.scientific.net/AMR.781-784.1685

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 781-784Characterization of Tomato Fruit LeRIN Gene...

Characterization of Tomato Fruit LeRIN Gene Application Virus-Induced Gene Silencing Technology

Abstract:

Virus-induced gene silencing (VIGS) is a powerful tool for the study of gene function and down regulate specific gene expression in plant. The RIN transcription factor is an important developmental regulator in tomato fruit ripening. In this work, the TRV-LeRIN infiltrated fruit significantly developed green color phenotypes, whereas the control fruit would turn red normally. Dissection of silenced areas for analysis identified whether the LeRIN gene is silenced or not. Taken together, we successfully applied syringe infiltration method of VIGS to silence the LeRIN gene in tomato fruit. These results are critical for understanding the mechanism of tomato fruit ripening.

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Periodical:

Advanced Materials Research (Volumes 781-784)

Pages:

1685-1688

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.781-784.1685

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Cite this paper

Online since:

September 2013

Authors:

Ling Li, Guo Xia Zhu, Zheng Liu, Tie Ling Liu

Keywords:

LeRIN, RIN Transcription Factor, Tomato Fruit, VIGS

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