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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 726-731Expression of CP:CBF3-35S:ICE1 Enhances Low...

Expression of CP:CBF3-35S:ICE1 Enhances Low Temperature Tolerance in Transgenic Arabidopsis

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Abstract:

We have constructed a vector pCAMBIA1300-CP:CBF3-35S:ICE1 and transformed into Arabidopsis. Results of PCR proved that the target genes had integrated into Arabidopsis genome. Transgenic Arabidopsis showed a bit slow growth, earlier flowering, but normal at other phenotype under 22°C with 8 h daily lights. In vitro low temperature stress tests showed that the transgenic lines were survival while the wild type was nearly dead. The transgenic plants also showed an increased proline content, SOD and POD activities under low temperature stress. The phenotype and physical evidence indicated that expression of CP:CBF3-35S:ICE1 under low temperature enhances the cold tolerance in transgenic plants.

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Periodical:

Advanced Materials Research (Volumes 726-731)

Pages:

118-121

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.726-731.118

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Cite this paper

Online since:

August 2013

Authors:

Rui Mei Li, Du Juan Xi, Yi Meng Ji, Rui Jun Duan, Jiao Liu, Shao Ping Fu, Xin Wen Hu, Jian Chun Guo

Keywords:

CBF3, Cold Resistance, ICE1

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