Molecular Cloning and Characterization Analysis of 3,8-Divinyl Protochlorophyllide a 8-Vinyl Reductase Gene from Dunaliella parva

Chang Hua Shang; Shun Ni Zhu; Zhen Hong Yuan; Zhong Ming Wang

doi:10.4028/www.scientific.net/AMR.347-353.3203

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 347-353Molecular Cloning and Characterization Analysis of...

Molecular Cloning and Characterization Analysis of 3,8-Divinyl Protochlorophyllide a 8-Vinyl Reductase Gene from Dunaliella parva

Abstract:

The vast majority of photosynthetic organisms utilize monovinyl chlorophyll for their photosynthetic reactions. For the biosynthesis of monovinyl chlorophyll, the reduction of the 8-vinyl group which is located on the B-ring of the macrocycle is essential. 3,8-Divinyl protochlorophyllide a 8-vinyl reductase (DVR) catalyzes the reduction of 8-vinyl group on the tetrapyrrole to an ethyl group, which is necessary for monovinyl chlorophyll (Chl) synthesis. The former studies indicated the DVR could enhance photosynthesis. The full-length cDNA encoding DVR was obtained from oleaginous microalgae Dunaliella parva, which include 1326 bp open reading frame (ORF), 22 bp 5′-untranslated sequence and 383 bp 3′-untranslated sequence. Dunaliella parva DVR showed the highest sequence similarity with the DVR from Chlamydomonas reinhardtii and Volvox carteri. The Dunaliella parva DVR also showed wide similarity with other species.

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Periodical:

Advanced Materials Research (Volumes 347-353)

Pages:

3203-3206

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.347-353.3203

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Online since:

October 2011

Authors:

Chang Hua Shang, Shun Ni Zhu, Zhen Hong Yuan, Zhong Ming Wang

Keywords:

Dunaliella Parva, DVR, Molecular Cloning, Sequence Analysis

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