A Novel Amperometric Immunosensor for Detection of Alpha-Fetoprotein Based on Magnetic and Electroactive Nanoprobes

Ling Hua Meng; Yu Ting Cao; Fu Tao Hu; Tian Hua Li; Ning Gan

doi:10.4028/www.scientific.net/AMR.345.411

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 345A Novel Amperometric Immunosensor for Detection of...

A Novel Amperometric Immunosensor for Detection of Alpha-Fetoprotein Based on Magnetic and Electroactive Nanoprobes

Abstract:

A novel three-dimensional, magnetic and electroactive nanoprobes were constructed for the first time. Using hemin (TPP) as electron mediator, mutli-walled carbon nanotubes with carboxyl groups (MCNTs) as supporter, a novel (MCNTs-TPP-Fe₃O₄)magnetic nanocomposites were first prepared. Then using alpha-fetoprotein (AFP) as model system, gold nanoparticles (Au NPs) as immobilizing matrix, the AFP/anti-AFP/Au NPs/ MCNTs-TPP-Fe₃O₄ nanoprobes were prepared and then dropped on the surface of screen-printed working electrode (SPCE) to construct a new amperometric immunosensors for detecting biomakers. The microstructure of different nanoparticles were observed by transmission electron microscopy (TEM) and X-ray fluorescence spectrometery (XRFS). Under optimal experimental conditions, the logarithm of response signal was proportional to the logarithm of AFP concentration from 0.1 to 200 ng/mL, with a correlation coefficient of 0.998. The detection limit was 0.04 ng/mL at a signal-to-noise ratio of 3. The proposed method offered a platform for fast, sensitive and simultaneous determination for serum samples.

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Periodical:

Advanced Materials Research (Volume 345)

Pages:

411-416

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.345.411

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Online since:

September 2011

Authors:

Ling Hua Meng, Yu Ting Cao, Fu Tao Hu, Tian Hua Li, Ning Gan

Keywords:

Alpha-Fetoprotein, MCNTs-TPP-Fe₃O₄, Nanoprobes, Screen-Printed Carbon Electrodes

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