The Development of Gelatin/Chitosan Hybrid Scaffold for Mouse Embryonic Stem Cell Line ATDC5

Yuan Li; Naoki Matsuda; Xu Xu Bao; Akira Teramoto; Koji Abe

doi:10.4028/www.scientific.net/AMR.123-125.395

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 123-125The Development of Gelatin/Chitosan Hybrid...

The Development of Gelatin/Chitosan Hybrid Scaffold for Mouse Embryonic Stem Cell Line ATDC5

Abstract:

Functional scaffolds fabricated from biopolymers are of great importance for tissue engineering. In this paper, by mimicking the combination of two major components in Extracellular Matrix – collagen and polysaccharide respectively, we fabricated gelatin and chitosan hybrid sponge for cartilage tissue engineering. Extending previous results of gelatin/chitosan film showed that all the hybrid coating film (chitosan: gelatin= 4:1; 1:1; 1:4) can support the adhesion of ATDC5 that comparable to Tissue Culture Dish (TCD). More importantly, stem cell line ATDC5 on coating dish of chitosan: gelatin=1:4 showed enhanced differentiation rate than that on other films and TCD. For three dimensional scaffolds, we fabricated hybrid sponge scaffold of chitosan: gelatin=4:1, 2:1, 1:1, 1:2, 1:4 respectively. Morphological characterizations of the sponges showed that this kind of gelatin/chitosan hybrid scaffold is promising for application in cartilage tissue engineering.

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Periodical:

Advanced Materials Research (Volumes 123-125)

Pages:

395-398

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.123-125.395

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Online since:

August 2010

Authors:

Yuan Li, Naoki Matsuda, Xu Xu Bao, Akira Teramoto, Koji Abe

Keywords:

ATDC5, Gelatin/Chitosan, Sponge, Stem Cell

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