Distribution & Access For Publication Downloads News About Us Contact Us
Paper Titles
First Principles Study on the Stability and Electronic Structures of 7,8-Dichloro-4-Oxo-4H-Chromene-3-Carbaldehyde
p.31
Isolation of Bacteria from Clarifier Effluent of Sour Pork Industry Able to Produce 1,3-Propanediol from Crude Glycerol
p.37
Influence of Organic Supplements on Growth and Development of In Vitro Shoots of Bulbophyllum dhaninivatii Seidenf
p.42
Inclusion Complex of Plai Oil and β-Cyclodextrin
p.47
Effect of Chitosan and Alginate Concentration on Size and Bactericidal Activity against Escherichia coli of Chitosan/Alginate/Silver Nanoparticle Beads
p.54
Inhibition of Herpes Simplex Virus Type 2 In Vitro by Durian (Durio zibethinus Murray) Seed Coat Crude Extracts
p.60
Nutritional Value and Anthocyanins of Mulberry and Roselle Mixed Fruits Jam
p.65
Effect of Different Cooking Method on Cooking Loss and Lipid Oxidation in Buffalo Meat
p.70
Nondestructive Determination of Maturity of the Monthong Durian by Mel-Frequency Cepstral Coefficients (MFCCs) and Neural Network
p.75

Effect of Chitosan and Alginate Concentration on Size and Bactericidal Activity against Escherichia coli of Chitosan/Alginate/Silver Nanoparticle Beads

Article Preview

Abstract:

Silver nanoparticles have been used in combination with biological polymer for antibacterial application. This study prepared chitosan/alginate/AgNP beads with varying chitosan and alginate concentration to use as an antibacterial material. The sizes of neat beads were larger (1286 ± 172, 1344 ± 142 and 1529 ± 73 μm for C1, C2 and C3, respectively) with increasing concentration of chitosan and alginate. Moreover, smaller beads were observed for the chitosan/alginate/AgNP beads, in which their sizes were 1151 ± 201, 1261 ± 204 and 1324 ± 198 µm for S1, S2 and S3, respectively, when compared to the chitosan/alginate beads. Furthermore, the minimum bactericidal concentration (MBC) of chitosan/alginate/AgNP beads against E. coli was 10, 10 and 3 µg/ml for S1, S2 and S3, respectively. This study suggested that the beads with the higher concentration of chitosan and alginate resulted in the greater bactericidal activity. Therefore, the chitosan/alginate/AgNP beads prepared in this study showed the bactericidal activity which can be used for antibacterial application.

By email Full Text Pdf
You have full access to the following eBook
URU International Conference on Science and Technology 2016 Read eBook

Info:

Periodical:

Applied Mechanics and Materials (Volume 855)

Pages:

54-59

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.855.54

Citation:

Cite this paper

Online since:

October 2016

Authors:

Dr.Pawika Mahasawat*, Ketsarin Hlongkeaw, Sutthida Charoenrit

Keywords:

Chitosan/Alginate/Silver Nanoparticle Beads, Escherichia coli, Silver Nanoparticles

Export:

RIS, BibTeX

Permissions:

Creative Commons CC BY 4.0

* - Corresponding Author

References

[1] R. Foldbjerg, P. Olesen, M. Hougaard, D.A. Dang, H.J. Hoffmann and H. Autrup, PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, apoptosis and necrosis in THP-1 monocytes. Toxicol Lett. 190 (2009) 156-162.

DOI: 10.1016/j.toxlet.2009.07.009

Google Scholar

[2] A. Ivask, I. Kurvet, K. Kasemets, I. Blinova, V. Aruoja, S. Suppi, H. Vija, A. Kakinen, T. Titma, M. Heinlaan, M. Visnapuu, D. Koller, V. Kisand and A. Kahru, Size-dependent toxicity of silver nanoparticles to bacteria, yeast, algae, crustaceans and mammalian cells in vitro. PLOS One. 9 (2014).

DOI: 10.1371/journal.pone.0102108

Google Scholar

[3] F. Martinez-Gutierrez, P.L. Olive, A. Banuelos, E. Orrantia, N. Nino, E.M. Sanchez, F. Ruiz, H. Bach and Y. Av-Gay, Synthesis, characterisation, and evaluation of antimicrobial and cytotoxic effect of silver and titanium nanoparticles. Nanomedicine. 6 (2010).

DOI: 10.1016/j.nano.2010.02.001

Google Scholar

[4] H.Y. Lee, H.K. Park, Y.M. Lee, K. Kim and S.B. Park, A practical procedure for producing silver nanocoated fabric and its antibacterial evaluation for biomedical applications. Chem Commun. 28 (2007) 2959-2961.

DOI: 10.1039/b703034g

Google Scholar

[5] K. Chaloupka, Y. Malam and A.M. Seifalian, Nanosilver as a new generation of nanoproduct in biomedical applications. Trends Biotechnol. 28 (2010) 580-588.

DOI: 10.1016/j.tibtech.2010.07.006

Google Scholar

[6] A.F. Martins, J.P. Monteiro, E.G. Bonafe, A.P. Gerola, C.T.P. Silva, E.M. Girotto, A.F. Rubira and E.C. Muniz, Bactericidal activity of hydrogel beads based on N, N, N-trimethyl chitosan/alginate complexes loaded with silver nanoparticles. Chin Chem Lett. 26 (2015).

DOI: 10.1016/j.cclet.2015.04.032

Google Scholar

[7] A. Travan, C. Pelillo, I. Donati, E. Marsich, M. Benincasa, T. Scarpa, S. Semeraro, G. Turco, R. Gennaro and S. Paoletti, Non-cytotoxic silver nnaoparticles-polysaccharide nanocomposites with antimicrobial activity. Biomacromolecules. 10 (2009).

DOI: 10.1021/bm900039x

Google Scholar

[8] J. Stojkovska, D. Kostic, Z. Jovanovic, M. Vukasinovic-Sekulic, V. Miskovic-Stankovic and B. Obradovic, A comprehensive approach to in vitro functional evaluation of Ag/alginate nanocomposite hydrogels. Carbohydr Polym. 111 (2014) 305-314.

DOI: 10.1016/j.carbpol.2014.04.063

Google Scholar

[9] A.F. Martins, H.D.M. Follmann, J.P. Monteiro, E.G. Bonafe, S. Nocchi, C.T.P. Silva, C.V. Nakamura, E.M. Girotto, A.F. Rubira and E.C. Muniz, Polyelectrolyte complex containing silver nanoparticles with antitumor property on Caco-2 colon cancer cells. Int J Biol Macromol. 79 (2015).

DOI: 10.1016/j.ijbiomac.2015.05.036

Google Scholar

[10] N.R. Jana, L. Gearheart and C.J. Murphy, Wet chemical synthesis of silver nanorods and nanowires of controllable aspect ratio. Chem Commun. (2001) 617-618.

DOI: 10.1039/b100521i

Google Scholar

[11] W.R. Li, X.B. Xie, Q.S. Shi, H.Y. Zeng, Y.S. Ou-Yang and Y.B. Chen, Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli, Appl Microbiol Biotechnol, 85 (2010) 1115-1122.

DOI: 10.1007/s00253-009-2159-5

Google Scholar

[12] W.R. Li, X.B. Xie, Q.S. Shi, S.S. Duan, Y.S. Ou-Yang, and Y.B. Chen, Antibacterial effect of silver nanoparticles on Staphylococcus aureus, Biometals, 24 (2011) 135-141.

DOI: 10.1007/s10534-010-9381-6

Google Scholar

[13] E. Torres, Y.N. Mata, M.L. Blazquez, J.A. Munoz, F. Gonzalez and A. Ballester, Gold and silver uptake and nanoprecipitation on calcium alginate beads. Langmuir. 21 (2005) 7951-7958.

DOI: 10.1021/la046852k

Google Scholar

[14] P. Pankongadisak, U.R. Ruktanonchai, P. Supaphol and O. Suwantong, Development of silver nanoparticles-loaded calcium alginate beads embedded in gelatin scaffolds for use as wound dressings. Polym Int. 64 (2015) 275-283.

DOI: 10.1002/pi.4787

Google Scholar

[15] P. Shi, P. He, T.K.H. The, Y.S. Morsi and J.C.H. Goh, Parametric analysis of shape changes of alginate beads. Powder Technology. 210 (2011) 60-66.

DOI: 10.1016/j.powtec.2011.02.023

Google Scholar

[16] A. Landriscina, J. Rosen and A.J. Friedman, Biodegradable chitosan nanoparticles in drug delivery for infectious disease. Nanomedicine. 10 (2015) 1609-19.

DOI: 10.2217/nnm.15.7

Google Scholar

Scientific.Net is a registered brand of Trans Tech Publications Ltd
© 2024 by Trans Tech Publications Ltd. All Rights Reserved