QCM Detection of HBV Based on Au-NPs/CNTs Signal Amplification

Kang Quan Qiao; Xiao Qun Guo; Yong Qiang Wu; Yi Guo He; Wei Hui Wang; Zhi Xu Ye

doi:10.4028/www.scientific.net/AMM.328.767

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 328QCM Detection of HBV Based on Au-NPs/CNTs Signal...

QCM Detection of HBV Based on Au-NPs/CNTs Signal Amplification

Abstract:

Gold nanoparticles/carbon nanotubes (Au-NPs/CNTs) composites synthesized by microwave-assisted method was modified on the gold coated quartz crystal by chitosan (CHIT) to immobilize anti-HbsAg antibody (HBsAb). Thus a HBsAb/(Au-NPs/CNTs)/CHIT/gold electrode was constructed and applied to the quantitative determination of Hepatitis B surface antigen (HBsAg) by quartz crystal microbalance (QCM). The obtained results show that Au-NPs/CNTs composites possessing high specific surface area is superior to gold nanoparticles (Au-NPs) in improving the detection sensitivity of HBsAg, and the detection sensitivity is 25.571 Hz/(μg/mL) and the linear range is 0.01 μg/mL ~ 5.0 μg/mL. The QCM immunosensor has good regenerability and not significant difference with ELISA in sample detection.

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Periodical:

Applied Mechanics and Materials (Volume 328)

Pages:

767-771

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.328.767

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Online since:

June 2013

Authors:

Kang Quan Qiao, Xiao Qun Guo, Yong Qiang Wu, Yi Guo He, Wei Hui Wang, Zhi Xu Ye

Keywords:

Gold Nanoparticles/Carbon Nanotubes, HBsAg, QCM

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