Morinda citrifolia leaves enhance osteogenic differentiation and mineralization of human periodontal ligament cells

Kanitsak BOONANANTANASARN; Kajohnkiart JANEBODIN; Prapan SUPPAKPATANA; Tawepong ARAYAPISIT; Jit-aree RODSUTTHI; Panjit CHUNHABUNDIT; Surintorn BOONANUNTANASARN; Wanida SRIPAIROJTHIKOON

doi:10.4012/dmj.2012-053-r

Original Paper

Morinda citrifolia leaves enhance osteogenic differentiation and mineralization of human periodontal ligament cells

Kanitsak BOONANANTANASARN, Kajohnkiart JANEBODIN, Prapan SUPPAKPATANA, Tawepong ARAYAPISIT, Jit-aree RODSUTTHI, Panjit CHUNHABUNDIT, Surintorn BOONANUNTANASARN, Wanida SRIPAIROJTHIKOON

Author information

Kanitsak BOONANANTANASARN
Department of Anatomy, Faculty of Dentistry, Mahidol University
Department of Oral Biology, School of Dental Medicine, The State University of New York at Buffalo
Kajohnkiart JANEBODIN
Department of Anatomy, Faculty of Dentistry, Mahidol University
Prapan SUPPAKPATANA
Department of Anatomy, Faculty of Dentistry, Mahidol University
Tawepong ARAYAPISIT
Department of Anatomy, Faculty of Dentistry, Mahidol University
Jit-aree RODSUTTHI
Department of Anatomy, Faculty of Dentistry, Mahidol University
Panjit CHUNHABUNDIT
Department of Anatomy, Faculty of Dentistry, Mahidol University
Surintorn BOONANUNTANASARN
Institute of Agricultural Technology, Suranaree University of Technology
Wanida SRIPAIROJTHIKOON
Department of Anatomy, Faculty of Dentistry, Mahidol University

Keywords: Morinda citrifolia, Osteogenic differentiation, Mineralization, Periodontal ligament cells

JOURNAL FREE ACCESS

2014 Volume 33 Issue 2 Pages 157-165

DOI https://doi.org/10.4012/dmj.2012-053-r

Details

Abstract

This present study investigated the potential of Morinda citrifolia leaf aqueous extract to induce osteogenic differentiation and matrix mineralization of human periodontal ligament (hPDL) cells. Human periodontal ligament cells were cultured in complete medium, ascorbic acid with β-glycerophosphate, or Morinda citrifolia leaf aqueous extract. Morinda citrifolia leaf aqueous extract significantly increased alkaline phosphatase activity compared to culturing in complete medium or ascorbic acid with β-glycerophosphate. Matrixcontaining mineralized nodules were formed only when the cells were cultured in the presence of Morinda citrifolia leaf aqueous extract. These nodules showed positive alizarin red S staining and were rich in calcium and phosphorus according to energy dispersive X-ray analysis. In conclusion, Morinda citrifolia leaf extract promoted osteogenic differentiation and matrix mineralization in human periodontal ligament cells, a clear indication of the therapeutic potential of Morinda citrifolia leaves in bone and periodontal tissue regeneration.

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