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Study of the interaction between HSA and oligo-DNA using total internal reflection ellipsometry

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Abstract

Techniques of quantitative analysis are very important for studies of the interactions between bio-molecules in the field of biotechnology and drug development. The total internal reflection ellipsometry system (TIRE) is an attractive label-free procedure for the quantitative analysis of biomolecules because it combines the analytic ability of ellipsometry and the high surface sensitivity of surface plasmon resonance. In this work, we have used TIRE to study the optical properties of an aquatic monolayer of human serum albumin (HSA) and oligo-DNA. Also, we have monitored the adsorption and the interaction processes of protein layers.

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Authors and Affiliations

  1. Nano-Optical Property Laboratory and Department of Physics, Kyung Hee University, Seoul, 130-701, Korea

    Y. W. Jung, J. S. Byun & Y. D. Kim

  2. Institute of Condensed Matter Physics, Faculty of Science, Masaryk University, Kotlářská 2, CZ-61137, Brno, Czech

    D. Hemzal & J. Humliček

Authors
  1. Y. W. Jung
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  2. J. S. Byun
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  3. Y. D. Kim
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  4. D. Hemzal
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  5. J. Humliček
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Corresponding author

Correspondence to Y. D. Kim.

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Jung, Y.W., Byun, J.S., Kim, Y.D. et al. Study of the interaction between HSA and oligo-DNA using total internal reflection ellipsometry. Journal of the Korean Physical Society 60, 1288–1291 (2012). https://doi.org/10.3938/jkps.60.1288

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  • DOI: https://doi.org/10.3938/jkps.60.1288

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