Enhancement of Phenolic Production and Antioxidant Activity from Buckwheat Leaves by Subcritical Water Extraction

Dong-Shin Kim; Mi-Bo Kim; Sang-Bin Lim

doi:10.3746/pnf.2017.22.4.345

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저널정보

한국식품영양과학회
Preventive Nutrition and Food Science 학술저널
Preventive Nutrition and Food Science Vol.22 No.4
2017.12 345 - 352 (8page)
DOI : 10.3746/pnf.2017.22.4.345

저자정보

Dong-Shin Kim (Jeju National University)
Mi-Bo Kim (Yonsei University)
Sang-Bin Lim (Jeju National University)

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2017

Enhancement of Phenolic Production and Antioxidant Activity from Buckwheat Leaves by Subcritical Water Extraction

Dong-Shin Kim , Mi-Bo Kim , Sang-Bin Lim Preventive Nutrition and Food Science 2017.12 학술저널

2016

Phenolic Compound Extraction from Buckwheat Leaves by Subcritical Water

Dong-Shin Kim , Mi-Bo Kim , Sang-Bin Lim 한국식품영양과학회 학술대회발표집 2016.10 학술대회자료

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To enhance the production of phenolic compounds with high antioxidant activity and reduce the level of phototoxic fagopyrin, buckwheat leaves were extracted with subcritical water (SW) at 100∼220℃ for 10∼50 min. The major phenolic compounds were quercetin, gallic acid, and protocatechuic acid. The cumulative amount of individual phenolic compounds increased with increasing extraction temperature from 100℃ to 180oC and did not change significantly at 200℃ and 220℃. The highest yield of individual phenolic compounds was 1,632.2 μg/g dry sample at 180oC, which was 4.7-fold higher than that (348.4 μg/g dry sample) at 100℃. Total phenolic content and total flavonoid content increased with increasing extraction temperature and decreased with increasing extraction time, and peaked at 41.1 mg gallic acid equivalents/g and 26.9 mg quercetin equivalents/g at 180℃/10 min, respectively. 2,2-Diphenyl-1-picrylhydrazyl free radical scavenging activity and ferric reducing ability of plasma reached 46.4 mg ascorbic acid equivalents/g and 72.3 mmol Fe²⁺/100 g at 180℃/10 min, respectively. The fagopyrin contents were reduced by 92.5∼95.7%. Color values L^* and b^* decreased, and a^* increased with increasing extraction temperature. SW extraction enhanced the yield of phenolic compounds with high antioxidant activity and reduced the fagopyrin content from buckwheat leaves.

#buckwheat leaves #subcritical water extraction #phenolic compounds #antioxidant activity #fagopyrin content