Effects of Endophytic Bacillus Subtilis and Salicylic Acid on Postharvest Diseases (Phytophthora infestans, Fusarium oxysporum) Development in Stored Potato Tubers
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effect of Endophytic B. subtilis (Strains 10-4, 26D) at Different Concentrations, Individually and in Compositions with SA, on the Development of Ph. infestans and F. oxysporum in Potato Tubers during Storage
2.2. The Ability of B. subtilis (10-4 and 26D) to Colonize the Internal Tissues of Potato Tubers Treated before Storage with B. subtilis and B. subtilis + SA under Normal and Pathogens (Ph. infestans and F. oxysporum)-Infected Stored Conditions
2.3. Change in Count of B. subtilis in Healthy and Infected (by Ph. infestans and F. oxysporum) Tubers over Storage Time and under Exogenous SA Influence
2.4. Effect of B. subtilis (10-4 and 26D), Individually and in Compositions with SA, on Lipid Peroxidation (MDA) in Stored Healthy and Pathogens (Ph. infestans and F. oxysporum)-Infected Potato Tubers
2.5. Effect of B. subtilis (10-4 and 26D) and B. subtilis + SA on Proline (Pro) Content in Healthy and Pathogens (Ph. infestans and F. oxysporum)-Infected Stored Tubers
2.6. Effect of Endophytic B. subtilis (10-4, 26D), and Their Compositions with SA, on Ascorbic Acid (AA) Content in Stored Potato Tubers
3. Materials and Methods
3.1. Plant Material, Scheme of Experiments, and Storage Conditions
3.2. Bacterial Strains and Phytopathogenic Fungi
3.3. Assessment Visual Symptoms of Disease Development
3.4. Determination of the Ability of B. subtilis to Colonize Internal Tissues of Potato Tubers
3.5. Assessment the Quantitative Content (Titer) of B. subtilis Bacteria in Potato Tubers
3.6. Quantification of Proline (Pro) Content
3.7. Estimation of Lipid Peroxidation (MDA)
3.8. Ascorbic Acid (AA) Determination
3.9. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Lastochkina, O.; Baymiev, A.; Shayahmetova, A.; Garshina, D.; Koryakov, I.; Shpirnaya, I.; Pusenkova, L.; Mardanshin, I.; Kasnak, C.; Palamutoglu, R. Effects of Endophytic Bacillus Subtilis and Salicylic Acid on Postharvest Diseases (Phytophthora infestans, Fusarium oxysporum) Development in Stored Potato Tubers. Plants 2020, 9, 76. https://doi.org/10.3390/plants9010076
Lastochkina O, Baymiev A, Shayahmetova A, Garshina D, Koryakov I, Shpirnaya I, Pusenkova L, Mardanshin I, Kasnak C, Palamutoglu R. Effects of Endophytic Bacillus Subtilis and Salicylic Acid on Postharvest Diseases (Phytophthora infestans, Fusarium oxysporum) Development in Stored Potato Tubers. Plants. 2020; 9(1):76. https://doi.org/10.3390/plants9010076
Chicago/Turabian StyleLastochkina, Oksana, Andrey Baymiev, Aysylu Shayahmetova, Darya Garshina, Igor Koryakov, Irina Shpirnaya, Liudmila Pusenkova, Il’dar Mardanshin, Cemal Kasnak, and Recep Palamutoglu. 2020. "Effects of Endophytic Bacillus Subtilis and Salicylic Acid on Postharvest Diseases (Phytophthora infestans, Fusarium oxysporum) Development in Stored Potato Tubers" Plants 9, no. 1: 76. https://doi.org/10.3390/plants9010076