Functional Characterization of the Lysine-Specific Histone Demethylases Family in Soybean
Abstract
:1. Introduction
2. Results
2.1. Identification and Characterization of GmLDLs
2.2. Expression Profile of GmLDLs
2.3. The Genetic Diversity of GmLDLs in Resequenced Soybean Accessions
2.4. Heterologous Expression and Enzymatic Assay of GmLDLs
3. Discussion
4. Materials and Methods
4.1. Identification of GmLDL Family Members
4.2. Bioinformatics Analysis of LDL Family Genes
4.3. Molecular Modeling
4.4. Expression Patterns of GmLDL Family Genes
4.5. Genotyping the GmLDL Family
4.6. Plant Materials and Treatments
4.7. Gene Cloning and Plasmid Construction
4.8. Recombinant Proteins Expression and Purification
4.9. Histone Demethylation Assay
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Synonymous SNP | NS SNP | InDel | NS SNP and InDel/kb | NS SNP and InDel at CD |
---|---|---|---|---|---|
GmFLD | 0 | 0 | 0 | 0 | 0 |
GmLDL1a | 0 | 5 | 1 | 2.72 | 2 |
GmLDL1b | 0 | 2 | 0 | 0.79 | 0 |
GmLDL2 | 0 | 2 | 0 | 0.90 | 0 |
GmLDL3a | 0 | 4 | 0 | 0.71 | 1 |
GmLDL3b | 0 | 2 | 0 | 0.35 | 1 |
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Liu, M.; Jiang, J.; Han, Y.; Shi, M.; Li, X.; Wang, Y.; Dong, Z.; Yang, C. Functional Characterization of the Lysine-Specific Histone Demethylases Family in Soybean. Plants 2022, 11, 1398. https://doi.org/10.3390/plants11111398
Liu M, Jiang J, Han Y, Shi M, Li X, Wang Y, Dong Z, Yang C. Functional Characterization of the Lysine-Specific Histone Demethylases Family in Soybean. Plants. 2022; 11(11):1398. https://doi.org/10.3390/plants11111398
Chicago/Turabian StyleLiu, Mengshi, Jiacan Jiang, Yapeng Han, Mengying Shi, Xianli Li, Yingxiang Wang, Zhicheng Dong, and Cunyi Yang. 2022. "Functional Characterization of the Lysine-Specific Histone Demethylases Family in Soybean" Plants 11, no. 11: 1398. https://doi.org/10.3390/plants11111398