Fe-S Protein Synthesis in Green Algae Mitochondria
Abstract
:1. Introduction
2. Fe and S Uptake and Metabolism in Algae
3. Synthesis of Fe-S Clusters
3.1. Cysteine Desulfurases
3.2. Scaffold Proteins
3.3. Regulatory Proteins
3.4. Chaperones and Co-Chaperones
3.5. Frataxin
3.6. CIA Pathway
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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ISC Proteins | NFS | ISU | NFU | ISD11 | HSCA | HSCB | ACP | FRATAXIN |
Function | Cysteine desulfurase | Scaffold protein | Scaffold protein | Regulatory protein | Chaperone | Co-chaperone | Regulatory protein | Iron donor |
Arabidopsis thalianagenes | At5g65720.1 | At4g22220 | At3g20970 (NFU4) At1g51390 (NFU5) | At5g61220 | At4g37910 (HSCA1) At5g09590 (HSCA2) | At5g06410 | At2g44620 (mtACP1) At1g65290 (mtACP2) At5g47630 (mtACP3) | At4g03240 |
UniProt | O49543-1 | O49627-1 | Q9LIG6-1 Q9C8J2 | Q8L9E3-1 | Q8GUM2 Q9LDZ0 | Q8L7K4 | P53665 O80800 Q9FGJ4 | Q9ZR07 |
Identified Protein Sequences | ||||||||
Organism | ||||||||
Auxenochlorella protothecoides | A0A087SBH3 (c) | RMZ55380.1 (c) | A0A087SIP0 | A0A087SND2 | XP_011400182 | A0A087SCC7 | RMZ53064.1 (c) | |
Bathycoccus prasinos | K8FF08 (p) | XP_007511837.1 | K8F7J7 | K8F1V7 | XP_007510865 | K8FET8 | XP_007511602.1 (p) | |
Chlamydomonas eustigma | GAX73632.1 | A0A250XNZ5 | A0A250XIU3 | GAX75929 | A0A250WTW6 | GAX72625.1 (c) | ||
Chlamydomonas reinhardtii | PNW80545.1 PNW87417.1 | XP_001693712.1 | A0A2K3D318 A0A2K3D340 | A8IZ28 | A8IZU0 (c) | A8IK66 | Q6UKY4 | A0A2K3D5B5 |
Chlorella sorokiniana | A0A2P6U3X4 | PRW56423.1 | A0A2P6TLS6 | A0A2P6U0I2 | A0A2P6TM18 | A0A2P6TMK0 (c) | A0A2P6TBX9 | |
Chlorella variabilis | XP_005843562.1 (c) XP_005848297.1 (c) | XP_005850086.1 (c) | E1ZCG7 (c) | E1ZMD2 | XP_005845092 (c) | E1Z6T8 | ||
Chloropicon primus | A0A5B8MHP9 | QDZ20066.1 | A0A5B8MCM2 | A0A5B8MHC3 | QDZ23877 | QDZ21836.1 | ||
Coccomyxa subellipsoidea | I0Z0B3 (c) | XP_005649671.1 | I0YUZ0 (c) | I0Z768 | I0YWN6 | I0YSD5 | XP_005644009.1 (c) | |
Dunaniella salina | KAF5838253 KAF5839176 (c) | KAF5837283.1 KAF5830450.1 KAF5832491.1 | KAF5832703.1 | KAF5833575.1 | ||||
Gonium pectorale | KXZ49215.1 | A0A150GMI8 | KXZ55931 (c) | A0A150GAM8 | KXZ42691.1 (c) | |||
Haematococcus lacustris | GFH16011.1 | A0A699ZQI7 | GFH07090 (c) | GFH14279.1 (c) | ||||
Helicosporidiumsp. | KDD73008.1 (c) | A0A059LST8 | A0A059LDR7 | |||||
Micractinium conductrix | A0A2P6V8I3 | PSC71357.1 (c) | A0A2P6V9W1 | A0A2P6VEW0 | A0A2P6VHZ4 | PSC73872 | A0A2P6VF08 | |
Micromonas commoda | XP_002506217.1 (c) XP_002501646.1 (c) | XP_002502856.1 XP_002507274.1 (c) | C1EHF7 | C1E8J2 | C1EGS6 | XP_002505982.1 | C1E2X1 | XP_002504509.1 |
Micromonas pusilla | XP_003055300.1 | C1MZU9 | C1MP69 | XP_003057174 | C1N9A6 | XP_003059868.1 (c) | ||
Monoraphidium neglectum | A0A0D2M031 (c) | XP_013897551.1 | XP_013898922 | A0A0D2MT62 | ||||
Ostreococcus lucimarinus | XP_001415848.1 | A4RUX0 | A4RYZ0 | A4RWG3 | XP_001419576 | A4S9X3 | XP_001416215.1 | |
Ostreococcus tauri | XP_003078141.1 XP_003081355.2 | XP_022838219.1 0US42676.1 | Q01C69 | A0A090M6V6 | Q01AH9 | XP_022839639 | Q00SL4 | XP_022840717.1 OUS42049.1 (c) |
Pycnococcus provasolii | GHP08054.1 | GHP08944.1 (p) | ||||||
Raphidocelis subcapitata | A0A2V0PAY6 | GBF95838.1 | A0A2V0PLS6 | A0A2V0PIR9 | GBF88744 | A0A2V0NV36 | GBF91763.1 | |
Scenedesmussp.NREL46B-D3 | KAF6244147.1 | KAF6263471 | KAF6260390.1 (c) | |||||
Scenedesmussp.PABB004 | KAF8055824.1 | KAF8065471 | ||||||
Tetrabaena socialis | PNH03155.1 | A0A2J8AFK7 | A0A2J8A4Z1 | A0A2J8A839 | PNH09606.1 | |||
Tetradesmus obliquus | A0A383VZF3 | A0A383V5U1 | A0A383WNF2 | A0A383W6Z2 | A0A383WKP1 | A0A383VDA2 | ||
Trebouxiasp. A1–2 | KAA6417420 | KAA6420365 (c) | KAA6425040.1 (p,m) | |||||
Volvox carterif. nagariensis | XP_002951960.1 | D8TNE6 | D8TVN4 | D8TMR1 (c) | XP_002948275 | D8U1A8 | XP_002950872.1 (c) |
NDOR1 | DRE2 | NBP35 | NAR1 | CIA1 | AE7 | MMS19 | |
Arabidopsis thaliana genes | At3g02280 | At5g18400 | At5g50960 | At4g16440 | At2g26060 | At1g68310 | At5g48120 |
UniProt | Q6NPS8 | Q8L7Z3 | Q8H1Q2-1 | Q94CL6 | 080990-1 | Q9C9G6 | Q0WVF8 |
Function | Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis. | Scaffold protein for formation of a [4Fe-4S] cluster | May play a role in the transfer of pre-assembled Fe/S clusters to target apoproteins | Transfer and insertion of Fe-S clusters into target proteins (CIA targeting complex) | Central member of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) pathway | Transfer and insertion of Fe-S clusters into specifics target proteins | |
Transfers electrons from NADPH to the Fe-S cluster of the DRE2 homolog | Facilitating the de novo assembly of a [4Fe-4S] cluster on the cytosolic Fe-S scaffold complex | ||||||
Identified protein sequences | |||||||
Organism | |||||||
Auxenochlorella protothecoides | A0A087SCD2 | A0A087SDS5 | A0A087SU81 | A0A087SMH1 | |||
Bathycoccus prasinos | K8EI89 | ||||||
Chlamydomonas eustigma | A0A250XRC9 | A0A250X446 | A0A250X674 | ||||
Chlamydomonas reinhardtii | Cre12.g551850.t1.2 | Cre11.g475350.t1.3 | Cre12.g494400.t1.2 | Cre03.g200550.t1.2 Cre03.g199800.t1.1 Cre09.g396600.t1.1 | Cre08.g374400.t1.3 | A0A2K3D8M0 | g14594.t1 |
Chlorella sorokiniana | A0A2P6TP36 | A0A2P6TVH6 | A0A2P6TVY6 | A0A2P6U4B7 | PRW58456.1 | ||
Chlorella variabilis | E1ZBV5 | E1ZFC7 | E1Z4N9 | E1ZKW8 | XP_005851493.1 | ||
Chloropicon primus | A0A5B8MC28 | A0A5B8MEG6 | A0A5B8MQB3 | ||||
Coccomyxa subellipsoidea | I0YJK0 | I0Z253 | I0YLU4 | I0YK24 | I0Z0I7 | ||
Gonium pectorale | A0A150GF73 | A0A150GJ11 | A0A150G6L9 | A0A150G9L7 | |||
Helicosporidium sp. ATCC 50920 | A0A059LKT0 | ||||||
Micractinium conductrix | A0A2P6VI63 | A0A2P6VKI3 | A0A2P6V477 | A0A2P6VJ54 | |||
Micromonas commoda | XP_002505452.1 | ||||||
Micromonas pusilla | C1N4X9 | XP_003059724.1 | |||||
Monoraphidium neglectum | A0A0D2MN30 | A0A0D2JM25 | |||||
Ostreococcus lucimarinus CCE9901 | A4RR54 | A4RRN3 | A4RRB8 | A4SBG2 | |||
Ostreococcus tauri | A0A096P7S5 | A0A090M2P3 | Q01GB5 | XP_003083112.2 | |||
Raphidocelis subcapitata | A0A2V0PD30 | A0A2V0P7V0 | A0A2V0PR28 | A0A2V0NRG7 | |||
Tetrabaena socialis | A0A2J8A236 | ||||||
Tetradesmus obliquus | A0A383VZJ9 A0A383W1G2 | A0A383W531 | A0A383VND8 | A0A383VR66 | |||
Volvox cateri f. nagariensis | D8UCR2 | D8U8M9 | D8UE05 | D8UBL6 | D8U2P0 | D8UEN0 |
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Gomez-Casati, D.F.; Busi, M.V.; Barchiesi, J.; Pagani, M.A.; Marchetti-Acosta, N.S.; Terenzi, A. Fe-S Protein Synthesis in Green Algae Mitochondria. Plants 2021, 10, 200. https://doi.org/10.3390/plants10020200
Gomez-Casati DF, Busi MV, Barchiesi J, Pagani MA, Marchetti-Acosta NS, Terenzi A. Fe-S Protein Synthesis in Green Algae Mitochondria. Plants. 2021; 10(2):200. https://doi.org/10.3390/plants10020200
Chicago/Turabian StyleGomez-Casati, Diego F., Maria V. Busi, Julieta Barchiesi, Maria A. Pagani, Noelia S. Marchetti-Acosta, and Agustina Terenzi. 2021. "Fe-S Protein Synthesis in Green Algae Mitochondria" Plants 10, no. 2: 200. https://doi.org/10.3390/plants10020200