Aqueous Extracts of Morus alba Root Bark and Cornus officinalis Fruit Protect against Osteoarthritis Symptoms in Testosterone-Deficient and Osteoarthritis-Induced Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Phenolic and Flavonoid Contents and Index Compounds
2.2. Bilateral Orchiectomies and Experimental Design
2.3. Diets and Experimental Design
2.4. MIA-Induced Osteoarthritis Animal Model and Osteoarthritis Progression
2.5. Pain-Related Behavior Assessments
2.6. Body Composition
2.7. Isolation of Total RNA from Articular Cartilage and Real-Time PCR
2.8. Histopathological Analysis of Knee Joints
2.9. Statistical Analysis
3. Results
3.1. Polyphenol and Flavonoid Contents of CFW and MBW
3.2. Global Observations of Osteoarthritis Symptoms
3.3. Pain-Related Behavior Tests for Osteoarthritis
3.4. The mRNA Expressions of Cytokines in Articular Cartilage of the OA Knee
3.5. Histopathological Analysis
3.6. Energy Metabolism and Body Composition
3.7. Glucose Metabolism
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
References
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Bioactive Components | CFW | MBW |
---|---|---|
Total polyphenol (mg GAE/g extract) | 42.7 ± 1.1 | 18.2 ± 0.4 |
Total flavonoids (mg QE/g extract) | 14.2 ± 0.7 | 2.3 ± 0.1 |
Gallic acid (μg/g extract) | 0.45 ± 0.03 | ND |
Morroniside (μg/g extract) | 11.0 ± 0.09 | ND |
Loganin (μg/g extract) | 6.2 ± 0.04 | ND |
Kuwanon G (μg/g extract) | ND | 2.2 ± 0.1 |
Morusin (μg/g extract) | ND | 1.6 ± 0.1 |
ORX-CON (n = 10) | ORX-CFW (n = 10) | ORX-MBW (n = 10) | Non-ORX-CON (n = 10) | |
---|---|---|---|---|
Bodyweight at 8th week (g) | 387 ± 18 b | 382 ± 19 b | 385 ± 25 b | 479 ± 18 a |
Bodyweight gain at 8th week (g) | 108 ± 13 b | 106 ± 13 b | 108 ± 12.9 b | 187 ± 21 a |
Bodyweight at 11th week | 374 ± 17 b | 373 ± 16 b | 375 ± 19 b | 452 ± 17 a |
Bodyweight gain between 8 and 11 weeks (g) | −13 ± 2.1 b | −9.0 ± 1.4 c | 10.1 ± 1.6 c | −26.8 ± 2.1 a |
Food intake (g/day) at 11th week | 17.3 ± 2.0 | 17.4 ± 2.2 | 18.4 ± 2.1 | 17.8 ± 1.5 |
Epididymal fat mass (g) | 7.4 ± 1.2 a | 6.1 ± 1.1 c | 4.7 ± 0.9 c | 7.0 ± 1.5 ab |
Serum glucose (mg/dL) at 11th week | 119 ± 14 a | 108 ± 11 ab | 105 ± 10 b | 110 ± 10 ab |
Serum insulin (ng/mL) at 11th week | 2.2 ± 0.5 a | 1.5 ± 0.5 b | 1.4 ± 0.4 c | 1.8 ± 0.5 ab |
HOMA-IR at 11th week | 7.8 ± 1.0 a | 4.8 ± 0.6 c | 4.4 ± 0.6 c | 5.9 ± 0.8 b |
Serum testosterone (ng/mL) at 11th week | 0.88 ± 0.11 c | 1.23 ± 0.16 b | 1.27 ± 0.17 b | 1.87 ± 0.10 a |
Serum TNF-α (pg/mL) | 68.4 ± 7.1 a | 56.5 ± 6.3 b | 50.1 ± 5.2 c | 59.3 ± 5.3 b |
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Park, S.; Moon, B.R.; Kim, J.E.; Kim, H.J.; Zhang, T. Aqueous Extracts of Morus alba Root Bark and Cornus officinalis Fruit Protect against Osteoarthritis Symptoms in Testosterone-Deficient and Osteoarthritis-Induced Rats. Pharmaceutics 2020, 12, 1245. https://doi.org/10.3390/pharmaceutics12121245
Park S, Moon BR, Kim JE, Kim HJ, Zhang T. Aqueous Extracts of Morus alba Root Bark and Cornus officinalis Fruit Protect against Osteoarthritis Symptoms in Testosterone-Deficient and Osteoarthritis-Induced Rats. Pharmaceutics. 2020; 12(12):1245. https://doi.org/10.3390/pharmaceutics12121245
Chicago/Turabian StylePark, Sunmin, Bo Reum Moon, Ji Eun Kim, Hyun Joo Kim, and Ting Zhang. 2020. "Aqueous Extracts of Morus alba Root Bark and Cornus officinalis Fruit Protect against Osteoarthritis Symptoms in Testosterone-Deficient and Osteoarthritis-Induced Rats" Pharmaceutics 12, no. 12: 1245. https://doi.org/10.3390/pharmaceutics12121245