Determination of the Infectious Agent of Translucent Post-Larva Disease (TPD) in Penaeus vannamei
Abstract
:1. Introduction
2. Results
2.1. Clinical Signs
2.2. Detection of Known Pathogens in the Diseased Samples
2.3. Bacterial Identification
2.4. Virulence Genes in the Isolated Strain
2.5. Pathogenicity of the Vibrio. Parahaemolyticus Determined by the Challenge Test
2.6. Histopathology Analysis of the Vibrio Infection
3. Discussion
4. Materials and Methods
4.1. Sample Collection and Experiment Shrimp
4.2. Detection of Eight Known Shrimp Pathogens in the Diseased Samples
4.3. Bacteria Isolation
4.4. Bacteria Identification
4.5. Analysis of Virulent Genes in the Isolated Strain
4.6. Experimental Challenge by Immersion
4.7. Histopathology
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene | Primer | Primer Sequences (5′-3′) | References |
---|---|---|---|
16S rDNA | 27F | AGAGTTTGATCMTGGCTCAG | [35,36] |
1492R | GGYTACCTTGTTACGACTT | ||
rpoD | rpoD-F | ACGACTGACCCGGTACGCATGTAYATGMGNGARATGGGNACNGT | [8] |
rpoD-R | ATAGAAATAACCAGACGTAAGTTNGCYTCNACCATYTCYTTYT | ||
rctB | rctB -F | ATHGARTTYACNGAYTTYCARYTNCAY | |
rctB-R | YTTNCTYTGHATNGGYTCRAAYTCNCCRTC | ||
toxR | toxR-F | GANCARGGNTTYGARGTNGAYGAYTC | |
toxR-R | TTDKKTTGNCCNCYNGTVGCDATNAC | ||
ldh | ldh-F | AAAGCGGATTATGCAGAAGCACTG | [29] |
ldh-R | GCTACTTTCTAGCATTTTCTCTGC | ||
pirAVp | pirA-284F | TGACTATTCTCACGATTGGACTG | [21] |
pirA-284R | CACGACTAGCGCCATTGTTA | ||
pirBVp | pirB-392F | TGATGAAGTGATGGGTGCTC | |
pirB-392R | TGTAAGCGCCGTTTAACTCA | ||
pirABVp | pirAB2020-F | GCACCGTAAATTTTCAGGTT | [27] |
pirAB2020-R | CGTTGCAATCTAAGACATAG | ||
AP4 | AP4-1 | AP4-F1: ATGAGTAACAATATAAAACATGAAAC | [37] |
AP4-R1: ACGATTTCGACGTTCCCCAA | |||
AP4-2 | AP4-F2: TTGAGAATACGGGACGTGGG | ||
AP4-R2: GTTAGTCATGTGAGCACCTTC |
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Zou, Y.; Xie, G.; Jia, T.; Xu, T.; Wang, C.; Wan, X.; Li, Y.; Luo, K.; Bian, X.; Wang, X.; et al. Determination of the Infectious Agent of Translucent Post-Larva Disease (TPD) in Penaeus vannamei. Pathogens 2020, 9, 741. https://doi.org/10.3390/pathogens9090741
Zou Y, Xie G, Jia T, Xu T, Wang C, Wan X, Li Y, Luo K, Bian X, Wang X, et al. Determination of the Infectious Agent of Translucent Post-Larva Disease (TPD) in Penaeus vannamei. Pathogens. 2020; 9(9):741. https://doi.org/10.3390/pathogens9090741
Chicago/Turabian StyleZou, Ying, Guosi Xie, Tianchang Jia, Tingting Xu, Chong Wang, Xiaoyuan Wan, Yingxia Li, Kun Luo, Xiaodong Bian, Xiuhua Wang, and et al. 2020. "Determination of the Infectious Agent of Translucent Post-Larva Disease (TPD) in Penaeus vannamei" Pathogens 9, no. 9: 741. https://doi.org/10.3390/pathogens9090741