Short-Chain Fatty Acids Promote Mycobacterium avium subsp. hominissuis Growth in Nutrient-Limited Environments and Influence Susceptibility to Antibiotics
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Culture
2.2. Static Biofilm Formation
2.3. Screening the Capacity of MAH104 Cells to Utilize a Range of Metabolites in Planktonic and Biofilm States
2.4. Testing the Effect of SCFA and Glycerol on Growth of MAH104 Planktonic Cultures in Nutrient-Restricted Media
2.5. Testing the Effect of SCFA and Glycerol on MAH104 Biofilm Cultures
2.6. Antibiotic Treatment of MAH Biofilms
2.7. MAH104 Infection and Antibiotic Treatment of Human Macrophages
2.8. Statistical Analysis
3. Results
3.1. MAH104 Displays a Decreased Capacity for Processing of Carbon Substrates in Biofilms
3.2. Glycerol and SCFA Support the Growth of Planktonic MAH104 in Nutrient-Limited Media
3.3. The Sessile MAH104 of Planktonic State Displays Higher Capacity for Growth and Biofilm Formation when Incubated with Glycerol or SCFA
3.4. Glycerol but Not SCFA Promote MAH104 Growth in Biofilms
3.5. Glycerol and SCFA Enhance Antibiotic Efficacy against MAH104 in Biofilms and in Cultured Macrophages
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Plate Well | Chemical Compound | Planktonic | Biofilm | Abiotic Reaction |
---|---|---|---|---|
PM1 plate | ||||
A1 | Negative control | - | - | - |
B3 | Glycerol | + | + | - |
C5 | Tween 20 | + | + | - |
C8 | Acetic acid | + | + | - |
D5 | Tween 40 | + | + | - |
D6 | α-Ketoglutaric acid | + | - | - |
D7 | α-Ketobutyric acid | + | - | - |
E5 | Tween 80 | + | + | - |
E7 | α-Hydroxybutyric acid | + | - | - |
F7 | Propionic acid | + | + | - |
G7 | Acetoacetic acid | + | - | - |
G9 | Monomethyl succinate | + | - | - |
G10 | Methyl pyruvate | + | + | - |
H8 | Pyruvic acid | + | + | - |
PM2A plate | ||||
A1 | Negative control | - | - | - |
D12 | Butyric acid | + | + | - |
E2 | Caproic acid | + | + | - |
F8 | Sebacic acid | + | + | - |
Treatment | CFU/mL at 14 Days | ||
---|---|---|---|
MAH104 | MAH3388 | MAH3393 | |
None | 4.2 ± 0.5 × 108 | 9.4 ± 0.2 × 107 | 1.0 ± 0.8 × 108 |
Clarithromycin | 8.9 ± 0.8 × 107 | - | - |
Amikacin | 5.8 ± 0.5 × 107 | 5.4 ± 0.6 × 106 | 5.2 ± 0.6 × 107 |
Glycerol | 1.1 ± 0.3 × 109 | n/a | n/a |
Glycerol + clarithromycin | 1.7 ± 0.2 × 104 * | - | - |
Glycerol + amikacin | 7.0 ± 0.2 × 105 * | n/a | n/a |
Propionic acid | 5.5 ± 0.4 × 106 | 9.6 ± 0.4 × 107 | 1.7 ± 0.4 × 107 |
Propionic acid + clarithromycin | 2.9 ± 0.3 × 103 * | - | - |
Propionic acid + amikacin | 3.6 ± 0.6 × 103 * | 3.1 ± 0.5 × 103 * | 1.6 ± 0.3 × 103 * |
Butyric acid | 3.4 ± 0.6 × 106 | 9.7 ± 0.2 × 107 | 6.2 ± 0.4 × 107 |
Butyric acid + clarithromycin | 2.7 ± 0.5 × 103 * | - | - |
Butyric acid + amikacin | 6.3 ± 0.7 × 103 * | 2.4 ± 0.5 × 103 * | 3.1 ± 0.5 × 103 * |
Caproic acid | 6.4 ± 0.2 × 106 | 8.4 ± 0.7 × 107 | 4.7 ± 0.3 × 107 |
Caproic acid + clarithromycin | 4.9 ± 0.6 × 103 * | - | - |
Caproic acid + amikacin | 7.3 ± 0.4 × 103 * | 4.1 ± 0.4 × 103 * | 1.6 ± 0.5 × 103 * |
Treatment | CFU/mL Cell Lysate | |
---|---|---|
2 h | 4 Days | |
None | 2.0 ± 0.4 × 105 | 8.2 ± 0.3 × 105 |
Clarithromycin | 3.9 ± 0.3 × 104 * | |
Amikacin | 4.8 ± 0.5 × 104 * | |
Glycerol | 2.3 ± 0.2 × 105 | 9.3 ± 0.3 × 105 |
Glycerol + clarithromycin | 8.3 ± 0.4 × 103 * | |
Glycerol + amikacin | 9.1 ± 0.5 × 103 * | |
Propionic acid | 3.1 ± 0.2 × 105 | 8.0 ± 0.2 × 105 |
Propionic acid + clarithromycin | 1.1 ± 0.3 × 104 * | |
Propionic acid + amikacin | 1.7 ± 0.3 × 104 * | |
Butyric acid | 3.0 ± 0.3 × 105 | 8.4 ± 0.5 × 105 |
Butyric acid + clarithromycin | 2.0 ± 0.4 × 104 * | |
Butyric acid + amikacin | 2.0 ± 0.3 × 104 * | |
Caproic acid | 2.4 ± 0.4 × 105 | 5.3 ± 0.4 × 105 |
Caproic acid + clarithromycin | 3.6 ± 0.2 × 104 * | |
Caproic acid + amikacin | 1.6 ± 0.4 × 104 * |
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Silva, C.A.d.M.e.; Rojony, R.; Bermudez, L.E.; Danelishvili, L. Short-Chain Fatty Acids Promote Mycobacterium avium subsp. hominissuis Growth in Nutrient-Limited Environments and Influence Susceptibility to Antibiotics. Pathogens 2020, 9, 700. https://doi.org/10.3390/pathogens9090700
Silva CAdMe, Rojony R, Bermudez LE, Danelishvili L. Short-Chain Fatty Acids Promote Mycobacterium avium subsp. hominissuis Growth in Nutrient-Limited Environments and Influence Susceptibility to Antibiotics. Pathogens. 2020; 9(9):700. https://doi.org/10.3390/pathogens9090700
Chicago/Turabian StyleSilva, Carlos Adriano de Matos e, Rajoana Rojony, Luiz E. Bermudez, and Lia Danelishvili. 2020. "Short-Chain Fatty Acids Promote Mycobacterium avium subsp. hominissuis Growth in Nutrient-Limited Environments and Influence Susceptibility to Antibiotics" Pathogens 9, no. 9: 700. https://doi.org/10.3390/pathogens9090700