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Review

Electrical Property of Graphene and Its Application to Electrochemical Biosensing

by
Jin-Ho Lee
1,2,
Soo-Jeong Park
3 and
Jeong-Woo Choi
1,3,*
1
Department of Chemical and Biomolecular Engineering, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 04107, Korea
2
Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
3
Research Center for Disease Biophysics of Sogang-Harvard, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul 04107, Korea
*
Author to whom correspondence should be addressed.
Nanomaterials 2019, 9(2), 297; https://doi.org/10.3390/nano9020297
Submission received: 31 January 2019 / Revised: 13 February 2019 / Accepted: 18 February 2019 / Published: 20 February 2019
(This article belongs to the Special Issue Electronic and Thermal Properties of Graphene)
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Abstract

:
Graphene, a single atom thick layer of two-dimensional closely packed honeycomb carbon lattice, and its derivatives have attracted much attention in the field of biomedical, due to its unique physicochemical properties. The valuable physicochemical properties, such as high surface area, excellent electrical conductivity, remarkable biocompatibility and ease of surface functionalization have shown great potentials in the applications of graphene-based bioelectronics devices, including electrochemical biosensors for biomarker analysis. In this review, we will provide a selective overview of recent advances on synthesis methods of graphene and its derivatives, as well as its application to electrochemical biosensor development. We believe the topics discussed here are useful, and able to provide a guideline in the development of novel graphene and on graphene-like 2-dimensional (2D) materials based biosensors in the future.

1. Introduction

Graphene, a single 2-dimensional (2D) layer of a hexagonal structure consisting of sp2 hybridized carbon atoms, and its derivatives have received increasing attention in biomedical fields, due to its unique physicochemical properties. This feature includes a high surface area, excellent electrical conductivity, strong mechanical strength, unparalleled thermal conductivity, and ease of surface functionalization (Table 1) [1,2,3,4,5].
Particularly, owing to the high surface area, excellent electrical conductivity, and capability to adsorb a variety of biomolecules, graphene has been considered as an ideal transducing material for constructing electrochemical biosensors [6,7]. It is well defined that the efficient electrochemical reaction takes place at the close distance between the electrode surface and the electroactive (reduction/oxidation, redox) site of a molecule. In detail, the electron transfer rate is inversely proportional to the exponential distance between the electrode surface and the electroactive redox site of the molecule [8,9]. Since the electron transfer between graphene and redox active molecule typically take place at either edge of the graphene layer or defects in the basal plane, the high surface area of 2D structure helps graphene to work as an excellent conducting material for electrical charge and heterogeneous electron transfer [10,11].
In addition, based on the unique atomic thin layer structure, the electrical properties of graphene are known to be highly sensitive to foreign atoms or absorbed molecules [12]. Favorably, graphene is known to be highly reliable for capturing aromatic molecules through a π–π stacking interaction. For example, single-stranded deoxyribonucleic acid (ssDNA) can bind to the graphene surface by π–π stacking interaction between deoxyribonucleic acid (DNA) and polyaromatic structures of graphene, and serve as a platform for various DNA based biosensing applications [13,14]. In addition, its derivatives graphene oxide (GO) are known to possess the oxygen-containing hydrophilic groups (hydroxyl and epoxy in the basal planes; carbonyl and carboxyl groups on the edges), that allows the electrostatic interaction [9,15]. Alternatively, graphene can be also functionalized by covalent bonding either through unsaturated p-bonds of graphene or oxygen-containing functional groups of GO [5,16]. As an example, various dienophiles, such as azomethine ylide, nitrene, and aryne, has been successfully generated a variety of terminal groups on the graphene surface for further modification [17,18,19]. The carboxyl groups of GO are used to link the amino groups of molecules by well-established carbodiimide chemistry [20]. According to the above mentioned unique physicochemical properties, the utilization of graphene as a functional component for an electrode has gained considerable interest in the field of electrochemical biosensors [19].
Although there exists an extensive collection of reviews for graphene synthesis methods and electrochemical sensing applications, the tremendous amount of recent activities and a new, live cell-based biosensing approach warrant a thorough review at this time. In this review, we will provide a selective overview of the recent advances on the synthesis methods of graphene and its derivatives, as well as its application in the electrochemical biosensor, which particularly covers small molecule, nucleic acid/protein, and live cell-based sensing. This review will provide an extensive analysis of the current state of the art and provide a perspective on key challenges that remain in the field. We hope that this review will inspire interest from various disciplines and highlight an important field wherein the advanced graphene-based electrochemical sensor is making great strides towards biomedical applications.

2. Synthesis of Graphene

As the outstanding physicochemical properties of graphene make this material promising candidate for electrochemical biosensor applications, the synthesis processes of the graphene which affect its properties also hold great influence on the proper development and performance of the biosensors. To this end, a number of a different synthesis method for graphene has been developed over the years. Particularly, in this review, most well-defined exfoliation phenomena or chemical vapor deposition (CVD) will be discussed as graphene and its derivatives synthesis methods (Figure 1) [9,21,22,23].

2.1. Mechanical and Chemical Exfoliation Method

The phenomena of exfoliation are to separate a few layers from the bulk material by overcoming the strong van der Waals attractions between adjacent layers. Among the exfoliation based process, the mechanical approach, ‘Scotch tape’ based exfoliation was first developed to obtain graphene, where few layers of graphene are peeled off from a highly ordered pyrolytic graphite (HOPG) flakes by external mechanical force (adhesive tape) based on the relatively weak interaction between the thin layers and the bulk materials. After the mechanical exfoliation, micromechanical cleavage of graphite, by rubbing a bulk crystal flake against another flake, was also utilized to obtain individual crystal planes of graphene layers [24]. In principle, through these mechanical exfoliation methods, high structural and electronic quality graphene crystals can be obtained without hosting structural defects into 2D graphene layers repeatedly [25,26,27]. However, these mechanical exfoliation methods are often limited for biosensor application, due to the difficulties in the mechanical cleavage of graphite crystals in a controlled manner. Such as low yield in the production of single-layer or few-layer graphene and relatively large lateral dimensions of graphene (range in micrometer size) often restrict their application in biosensors. Apart from the mechanical exfoliation, the potential energy caused by van der Waals attractions could be overcome in the presence of solvents as well [28]. This process, which is also known as liquid phase exfoliation (LPE), generally requires dispersion of bulk materials in a solvent, exfoliation, and purification [29]. Thus, the selection of ideal solvents is critical for LPE process to obtain high yield and stability [30]. However, the commonly used solvents for LPE, such as N,N-dimethylformamide (DMF) and N-methyl pyrrolidinone (NMP) are usually known to have acute toxic effects. To overcome this limitation, numerous approaches, such as urea-based aqueous exfoliation, which even showed higher efficiency compare to conventional DMF based exfoliation, were recently developed [31]. In addition, graphene derivatives can be also synthesized by the chemical oxidation of graphite. For example, one of most well established Hummer’s method for preparing graphite oxide includes the addition of potassium permanganate (KMnO4) to a mixture of graphite, sodium nitrate (NaNO3), and concentrated sulfuric acid (H2SO4) [32]. During the oxidation, small ions intercalate to bulk graphite oxide during oxidation and weaken the interlayer interactions. In detail, the sp2 hybridized carbon bonding is disrupted during the oxidation process, yielding formation of sp3 hybridized carbon bonding. Through this mechanism, sonication allows exfoliating GO layer from bulk graphite oxide flake and subsequent reduction of GO could also help to obtain reduce graphene oxide (rGO) [33,34,35]. Owing to ease of process, the exfoliation based on chemical method is known to be suitable for the synthesis of graphene at a large scale, which is important for the construction of bioelectronics devices, including electrochemical biosensors. In addition, the unique chemical structure of chemically derived GO and rGO, which differs from the pristine graphene or graphite, provide versatility in applications based on the chemical functionalization through the oxygen functional groups. However, large amounts of structural defects caused by inevitably introduced oxygen functional groups could affect the electrical properties of graphene and the performance of electrochemical biosensors.

2.2. Thermal Decomposition and Chemical Vapor Deposition Method

An alternative approach to synthesize a high quality graphene layer in a large scale comprises the self-organization of carbon atoms on the surface of the crystal by the thermal decomposition of hydrocarbon and segregation of the carbon monolayer on metal substrates through CVD [36,37,38]. For example, the evaporation of silicon from single-crystalline silicon carbide (SiC) substrates at a high annealing temperature results in the organized attachment of the remaining carbon atoms on the surface of lattice-matched SiC substrates. Though, this method directly provides graphene layers on insulating SiC substrates in a wafer-scale; however, the strong interaction of graphene with substrates limits doping property, as well as transfer efficiency to other substrates for biomedical applications. Among the numerous metal substrates, nickel (Ni) and (copper) Cu substrates demonstrated the potential to separate graphene layer from the substrates and transfer onto other substrates, including a solid substrate to flexible and bendable substrates [36]. In detail, the synthesis mechanism for the metals substrate with high solubility of carbon species, such as Ni substrate include catalytic decomposition of the precursor, dissolution of decomposed carbon species, segregation of dissolved carbon atoms onto the metal surface, and followed by nucleation and growth of graphene layer on the surface of the substrate [39]. Though, the several critical factors, such as the thickness of Ni films, growth time, and cooling rate has been already revealed to improve the quality of synthesized graphene layers; however, it is still difficult to obtain a single layer of graphene through the polycrystalline Ni substrate and the electrical property of the synthesized graphene layer were found to be not very satisfactory as well. Comparably, by using a Cu substrate, which has low solubility of carbon, highly uniformed single graphene layer with the excellent electrical property was synthesized [36,40]. Due to the low solubility of carbon, the formation of graphene happens through the self-limited nucleation and lateral growth by diffusion of carbon atoms on the surface directly after the decomposition of precursors [41]. In addition, free-floating graphene layers could be obtained by metal etching or electrochemical bubbling method for further applications [42]. Although, CVD method can synthesize graphene layer even in a large area (up to several inches) with high electronic quality (mobility up to 105 cm2V−1s−1) comparable to the exfoliation methods [36,39,43], the structural defects or contamination which can be originated during the etching and transfer process are still limiting factor for obtaining high profile graphene layer and can also affect the performance of electrochemical biosensors as well.

3. Application to Electrochemical Sensing

The biosensor is the analytical device which consists of a biological component that recognizes the target analytes and an electrical component (transducer) which converts the recognition event into a measurable signal. To improve the performance of biosensors, tremendous efforts from multidiscipline fields has been established. Ever since the discovery of graphene by Geim and Novoselov in 2004, numerous approach has been conducted to utilize graphene as transducing material to improve the performance of electrochemical sensors [6,44]. Graphene and its derivatives modified electrodes have exhibited excellent electrochemical behavior in terms of their high surface area and active electron transfer sites [7], which makes graphene as a promising electrode material to improve the performance of graphene and its derivatives based electrochemical biosensors. In this review, the division of biomedical electrochemical sensors will be divided into three categories i) small molecules, ii) nucleic acids and proteins, and iii) Live cell-based sensing.

3.1. Small Molecule Sensing

There are many small molecules that are highly relevant to human health and disease. Even a subtle change of these biomolecules could cause a serious disease which threatens a patient’s life [45,46,47,48]. For example, dopamine (DA) is one of the most important neurotransmitters that play a vital role in the central nervous system. Abnormal level of DA can cause severe neurological disorders, such as Parkinson’s disease [49,50,51]. However, due to the complex matrices of nature, it is still challenging to develop a biosensor to distinguish the biomolecules which share a similar oxidation potential, such as ascorbic acid (AA), uric acid (UA) and other catecholamine molecules. To resolve this problem, graphene has been adopted as a transducing material in the development of the electrode. Through its phenyl structure, these molecules could adsorb on the graphene-modified electrode surface through the different π-π stacking interactions. Ping et al. introduced a graphene-based screen-printing ink which could selectively and sensitively analyze these molecules via differential pulse voltammetry (DPV) [52]. Even in the co-existence of these three molecules, the linear range and detection limit were found to be 0.5–2000 μM, 4.0–4500 μM, and 0.8–2500 μM and 0.12 μM, 0.95 μM, and 0.20 μM for DA, AA, and UA, respectively.
As an alternative approach, graphene was also utilized to provide large surface area, as well as a binding motif for the mediator (i.e., enzyme) to improve the electrocatalytic performance of electrochemical sensors to determine small molecules, such as hydrogen peroxide (H2O2), glucose, and nicotinamide adenine dinucleotide (NADH) sensitively. Besides its well-known cytotoxic effects, as an essential mediator in many biological processes, H2O2 detection has earned great attention. However, due to the co-existing other electro-active constituents, the detection of H2O2 is easily interfered [53,54]. To improve the H2O2 detection efficiency, graphene was also utilized to improve the performance of electrochemical sensors. Fan et al., designed a graphene capsule, which served as a carrier for horseradish peroxidase (HRP), to detect H2O2 in human serum [53]. Through the large surface area and high conductivity of graphene, the synergistic effect on catalytic activity was able to be obtained. Instead of using the enzyme, Wang et al., have grown Prussian blue nanocubes on the surface of nitrobenzene-functionalized reduced graphene oxide as an “artificial enzyme peroxidase” for constructing H2O2 electrochemical biosensors [55]. Another approach based on the incorporation of myoglobin (Mb) on graphene oxide encapsulated molybdenum disulfide (MoS2) nanoparticle (Mb-GO@MoS2) hybrid structure was also reported by Choi’s group (Figure 2) [56]. Mb is a one of metalloprotein family with unique redox properties, due to the metal ion core integrated into the hemin group. Through this unique redox properties, Mb can be used to detect H2O2 through electrochemical reduction of H2O2 as well. The developed Mb-GO@MoS2 structure and extended electroactive surface also affected the fast electron transfer and resulted in an enhanced amperometric response H2O2. Instead of using full protein, Song et al. utilized hemin porphyrin and functionalized on the graphene/GNP/glassy carbon electrode to avoid the possible insulating effect from protein structure [57]. Note that hemin porphyrin is a well-known natural metalloporphyrin which is the active site in heme-proteins, such as hemoglobins and myoglobins [58]. Alternatively, Shao et al. have shown that by just doping graphene nitrogen, the better electrocatalytic activity could be obtained for H2O2 detection as well [59]. The enhanced performance of nitrogen-doped graphene is expected, due to the existence of nitrogen functional groups in addition to oxygen-containing groups and structural defects. In a similar manner, graphene was also utilized to improve the electrocatalytic performance of electrochemical sensors to determine other small molecules, such as DA, glucose, and NADH as well. The recent researches on graphene-based electrochemical biosensors toward various small molecules are compared in Table 2.

3.2. Nucleic Acid and Protein Sensing

A sensitive and selective nucleic acids (DNA/ribonucleic acid, RNA) sensor is in high demand for the diagnosing gene-related diseases. DNA sensors also referred to as geno-sensors, are an analytical system, which integrates a sequence-specific probe on a transducer. Thus, the immobilization of DNA strands greatly influences the performance of the electrochemical DNA sensor. In this manner, graphene and its derivatives provide an excellent avenue to develop electrochemical DNA sensor. Hu et al. utilized GO as a DNA probe immobilization layer for electrochemical detection of HIV-1 gene fragment [70]. First, GO is anchored on diazonium functionalized electrode surface via electrostatic attraction, hydrogen bonding or epoxy ring opening. The π-π stacking interaction between the aromatic ring of GO and DNA base ring facilitated DNA immobilization, and impedance measurement was used for the quantitative detection of HIV-1 gene fragment up to 0.11 pM. Moreover, Akhavan et al. developed a graphene nanowall structure and showed an extremely high response to single-strain DNA towards single-strain DNA electrochemical sensing. As a result, they have observed a unique response signal from each kind of basic group through DPV measurement (Figure 3) [71].
Although it is clear that nucleic acids can effectively immobilize on the graphene and its derivatives, many researchers have focused to modify the graphene and its derivatives electrode surface with various materials to achieve improved performance on electrochemical sensor. For example, Tiwari et al. electrophoretically deposited graphene oxide modified iron oxide-chitosan hybrid nanocomposite onto indium tin oxide (ITO) coated glass substrate and utilized for the detection of a pathogenic Escherichia coli DNA with a detection limit of 10 fM [72]. In addition to the surface modification, the enzyme was also utilized to improve electrochemical sensor. Esteban-Fernández de Ávila et al. designed a disposable electrochemical DNA sensor based on carboxymethyl-cellulose-rGO modified screen-printed carbon electrodes. And HRP was utilized to catalyze the redox mediator, tetramethylbenzidine (TMB), and the substrate (H2O2) for the detection of the p53 tumor suppressor gene [73]. Besides, graphene can be also utilized for DNA sequencing. With the presence of nanopore on the monolayer graphene, detailed electric signals can be sensed when DNA passes the pore by measuring transverse conductance of DNA. Through this mechanism, Freedman et al. have distinguished long and short DNA using nanopores with graphite polyhedral crystal (GPC)-edges (Figure 4) [74].
In parallel, many biological processes can be also monitored by quantification of specific proteins. Owing to the amphiphilic nature of graphene and its derivate, it provides sufficient active sites to immobilize these probes, including aptamer and antibody to detect specific proteins as well. Wen et al. explored hairpin-shaped DNA aptamer as a cognition element for carcinoembryonic antigen on the gold nanorods functionalized graphene electrode surface [75]. In addition, by targeting membrane protein of pathogenic microbes, Natarajan et al. successfully developed an immunoassay for white spot syndrome virus using a methylene blue dye (MB) immobilized graphene oxide modified glassy carbon electrode (GCE/GO@MB) (Figure 5) [76]. Here, graphene was also utilized to improve antibody immobilization efficiency and enhance electron transfer, the binding on the target produced an enhanced immune-recognition response by the sandwich assay with an enzyme reaction. As mentioned above, considerable approaches have been made to develop and improve graphene-based electrochemical DNA and protein sensors. However, the simultaneous detection of multiple targets in complex biological matrices still remains a major bottleneck for clinical analysis. The recent researches on graphene-based electrochemical biosensors toward various protein and nucleic acids are compared in Table 3.

3.3. Live Cell-based Sensing

Owing to their excellent biocompatibility, solubility, and unique interactions with specific molecules, graphene, and its derivatives has been also utilized to detect a response from the biological process of living cells. For example, effective and accurate characterization of H2O2 concentration in a living cell is critical to achieving the normal physiological activities of cells. Wu et al. integrated nitrogen-doped graphene to monitor H2O2 release process from live cells through improved electrocatalytic activity [84]. After the injection of phorbol 12-myristate-13-acetate to induce H2O2 generation in the neutrophil cells, the rapid increase of amperometric response was able to be observed, which indicates a large amount of H2O2 release from the cells. Sun et al., also reported a graphene/Intermetallic platinum/lead (Pt/Pb) nanoplates composites for sensing H2O2 release from live macrophage cells (Raw 264.7) (Figure 6) [85]. Through the high-density of electrocatalytic active sites on the unique PtPb nanoplates and the synergistic effect with graphene contributed for outstanding electroanalytical performance. The proposed construct showed 12.7 times higher redox signals than that of commercial Pt/Carbon electrode and able to detect H2O2 with a wide linear detection range of 2 nM to 2.5 mM. Zhang et al. also proposed a way to monitor H2O2 secretion from viable cells with a freestanding nanohybrid paper electrode composed of 3D ionic liquid (IL) functionalized graphene framework (GF) decorated by gold nanoflowers [86]. The gold nanoflower modified IL–GF was synthesized by a dopamine-assisted one-pot self-assembly method. The resultant nanohybrid paper electrode exhibits good non-enzymatic electrochemical sensing performance toward H2O2. Through the real-time tracking of H2O2 release from different breast cells attached to the paper electrode allow to distinguish the normal breast cell line HBL-100 from the cancer breast cell line MDA-MB-231 and MCF-7 cells. Liu et al. utilized HRP on a porous graphene electrode to monitor the H2O2 release from living cells [87]. A simple method based on silver nanoparticles etching process was proposed to prepare porous graphene network. Owing to the versatile porous structure, the analysis performance was significantly improved by loading large amounts of enzyme and accelerating diffusion rate. A significant low detection limit of 0.0267 nM and wider linear range of 7 orders of magnitude were achieved. A rat adrenal medulla pheochromocytoma cell line PC12 was chosen as a model cancer cell, and H2O2 release was monitored within AA stimulation. In a similar manner, Li et al. monitored nitric oxide (NO) by developing a new 3D hydrogel composite via in situ reductions of Au3+ on three-dimensional graphene hydrogel [88]. The developed sensor showed improved electrochemical performance compare to pure gold nanoparticles, pure graphene, 3D graphene hydrogels, and gold nanoparticle-graphene hybrids. A linear relation was obtained for 0.05–0.4 mM of NO. Two different normal and cancer skin cell was stimulated with Ach, and concentration-dependent signal increments were analyzed.
Differently, Lee et al., utilized graphene-Au hybrid nanoelectrode array (NEAs) to monitor stem differentiation in a non-destructive real-time manner (Figure 7) [89]. Typically, unique multifunctional graphene-Au hybrid NEAs were fabricated via laser interference lithography and physical vapor deposition methods. Followed by surface modification with reduced graphene oxide. The presence of reduced graphene oxide enhanced the cell adhesion and spreading without functionalization with any extracellular matrix proteins, which could work as an insulator and diminish ET between the electrode and electroactive molecules. Owing to the excellent biocompatibility and electrochemical performance of graphene-Au hybrid NEAs, the osteogenic differentiation of human mesenchymal stem cell was successfully monitored through an alkaline phosphatase (ALP)-based enzymatic reaction. During the osteogenesis, ALP expression level is known to be sequentially increased. P-aminophenyl phosphate (PAPP) were introduced to cell prior to electrochemical monitoring, the ALP expressed on the cell catalytically hydrolyzed the PAPP to produce electroactive p-aminophenol (PAP), and the redox reaction between PAP and Quinone imine (QI) was monitored by cyclic voltammogram. Through this mechanism, the osteogenic differentiation of human mesenchymal stem cell was successfully monitored in both non-destructive and real-time manner. Although, stem cell therapy has arisen as a promising method in the field of biomedicine owing to their unique ability to differentiate into multiple cell lineages [90], necessary required destructive analysis process, such as cell lysis and cell fixation were one of a critical limiting factor for further clinical applications. Such a novel electrochemical detection method proposed by graphene-Au hybrid NEAs could be a breakthrough in the preclinical investigation of differentiated stem cells. Consequently, this kind of work is expected to be highly potential to advance stem cell differentiation assays by providing a practical, non-destructive, real-time monitoring tool. The recent researches on graphene-based electrochemical biosensors toward various live cell-based sensing strategies are compared in Table 4.

4. Conclusions and Future Outlook

It is evident that the exceptional physicochemical properties of graphene and its derivatives make them compelling for various electrochemical biosensor applications. The development and wide application of the electrochemical sensing based on these materials were hindered by the lack of facile and reproducible synthesis method of these materials with defined properties. In order to make it compatible to develop various electrochemical sensors, numerous efforts have been devoted to controllable and scalable production of graphene and its derivatives. Mechanical exfoliation method was first used to obtain graphene; however, the poor controllability and low production were the limiting factors. To achieve scalable production, LPE based methods were developed to obtain a dispersed solution of these materials and its products has been the most widely utilized for the electrochemical biosensors. More or less, recently CVD method was also adopted to synthesize graphene with controllable properties; however, limited scale production of graphene and its derivatives still hinders its applications. For example, various oxygenated surface functional groups provide a relatively high surface area, while it inhibits the performance of electrochemical biosensor as an insulator. Thus, a novel synthesis method which can consistently produce graphene and its derivatives with defined properties (high quality) in a large scale (high yield) and cost-effective manner is still required for future commercialization of graphene and its derivatives based electrochemical biosensors.
During the past years, many different strategies have been also explored to develop novel graphene and its derivatives based electrochemical biosensors for analyzing bio/chemical molecules. It is clear that the optimization through the selection of a suitable surface functionalization method still needs to be revealed, in order to develop a highly effective and reproducible electrochemical biosensors. In addition, the combination with functional materials, such as ionic liquids, nanomaterials and polymers, have provided numerous choices to take the synergistic effect to enhance the electroanalytical performances. However, designing and finding the appropriate combinatorial structure with functional materials must be addressed for each biomolecule to maximize the performance of electrochemical biosensors. Although extensive studies have been made to design and fabricate novel electrochemical biosensors, long-term stability of combinatorial structure and functionalized surfaces (with receptor), in complexed real sample matrices, should be considered. Real biological fluids, such as blood and plasma, always contain various molecules and ions which can cause the interference through nonspecific binding events and undermine the performance of electrochemical biosensors. Furthermore, to adopt graphene and its derivatives for recently arisen in situ live cells and in vivo sensing strategies, more effort, such as long-term toxicity of graphene and its derivatives, as well as incorporated functional materials, should be also discovered. Moreover, since graphene and its derivatives can serve as both the sensing component and transducer, utilization to flexible electrode and miniaturization in size by forming free-standing structures via self-assembly would be an excellent approach for developing an implantable (flexible) and portable (lightweight) sensors as well. However, the lack of a suitable power source is a limiting factor.
In addition, owing to the advantages of specific planar morphology, graphene-like 2D nanomaterials have attracted significant attention as emerging materials for electrochemical sensor approaches. Taking the advantages of diverse composition and structural effect, graphene-like 2D nanomaterials also have promoted great efforts to improve the performance of electrochemical biosensors. Similar to graphene and its derivatives, the defined synthesis method for high quality graphene-like 2D nanomaterials with controllable sizes and thickness, as well as tunable properties, and surface functionalization method is highly desirable for their practical application to electrochemical biosensor development as well. In witness of their current attention, we also endeavor that further improvement of graphene and its derivatives, as well as graphene-like 2D nanomaterial-based electrochemical biosensors, will lead to a significant advance in analytic applications for the highly effective and reliable detection of biomarker and open new avenues in biological and medical fields.

Funding

This research was supported by the Leading Foreign Research Institute Recruitment Program, through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT and Future Planning (MSIP) (2013K1A4A3055268) and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1A6A1A03012845).

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Stoller, M.D.; Park, S.; Zhu, Y.; An, J.; Ruoff, R.S. Graphene-based ultracapacitors. Nano Lett. 2008, 8, 3498–3502. [Google Scholar] [CrossRef] [PubMed]
  2. Weiss, N.O.; Zhou, H.; Liao, L.; Liu, Y.; Jiang, S.; Huang, Y.; Duan, X. Graphene: An emerging electronic material. Adv. Mater. 2012, 24, 5782–5825. [Google Scholar] [CrossRef] [PubMed]
  3. Lee, C.; Wei, X.; Kysar, J.W.; Hone, J. Measurement of the elastic properties and intrinsic strength of monolayer graphene. Science 2008, 321, 385–388. [Google Scholar] [CrossRef] [PubMed]
  4. Balandin, A.A.; Ghosh, S.; Bao, W.; Calizo, I.; Teweldebrhan, D.; Miao, F.; Lau, C.N. Superior thermal conductivity of single-layer graphene. Nano Lett. 2008, 8, 902–907. [Google Scholar] [CrossRef] [PubMed]
  5. Georgakilas, V.; Otyepka, M.; Bourlinos, A.B.; Chandra, V.; Kim, N.; Kemp, K.C.; Hobza, P.; Zboril, R.; Kim, K.S. Functionalization of graphene: Covalent and non-covalent approaches, derivatives and applications. Chem. Rev. 2012, 112, 6156–6214. [Google Scholar] [CrossRef] [PubMed]
  6. Song, Y.; Luo, Y.; Zhu, C.; Li, H.; Du, D.; Lin, Y. Recent advances in electrochemical biosensors based on graphene two-dimensional nanomaterials. Biosens. Bioelectron. 2016, 76, 195–212. [Google Scholar] [CrossRef] [PubMed]
  7. Bollella, P.; Fusco, G.; Tortolini, C.; Sanzo, G.; Favero, G.; Gorton, L.; Antiochia, R. Beyond graphene: Electrochemical sensors and biosensors for biomarkers detection. Biosens. Bioelectron. 2017, 89 Pt 1, 152–166. [Google Scholar] [CrossRef]
  8. Rusling, J.F. Enzyme bioelectrochemistry in cast biomembrane-like films. Acc. Chem. Res. 1998, 31, 363–369. [Google Scholar] [CrossRef]
  9. Lawal, A.T. Synthesis and utilisation of graphene for fabrication of electrochemical sensors. Talanta 2015, 131, 424–443. [Google Scholar] [CrossRef]
  10. Pumera, M. Graphene in biosensing. Mater. Today 2011, 14, 308–315. [Google Scholar] [CrossRef]
  11. Ostrovsky, P.M.; Gornyi, I.V.; Mirlin, A.D. Electron transport in disordered graphene. Phys. Rev. B 2006, 74, 235443. [Google Scholar] [CrossRef]
  12. Schedin, F.; Geim, A.K.; Morozov, S.V.; Hill, E.W.; Blake, P.; Katsnelson, M.I.; Novoselov, K.S. Detection of individual gas molecules adsorbed on graphene. Nat. Mater. 2007, 6, 652–655. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  13. Lu, C.; Huang, P.J.; Liu, B.; Ying, Y.; Liu, J. Comparison of Graphene Oxide and Reduced Graphene Oxide for DNA Adsorption and Sensing. Langmuir 2016, 32, 10776–10783. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  14. Hwang, M.T.; Landon, P.B.; Lee, J.; Choi, D.; Mo, A.H.; Glinsky, G.; Lal, R. Highly specific SNP detection using 2D graphene electronics and DNA strand displacement. Proc. Natl. Acad. Sci. USA 2016, 113, 7088–7093. [Google Scholar] [CrossRef] [PubMed]
  15. Liu, J.; Liu, Z.; Barrow, C.J.; Yang, W. Molecularly engineered graphene surfaces for sensing applications: A review. Anal. Chim. Acta 2015, 859, 1–19. [Google Scholar] [CrossRef] [PubMed]
  16. Xu, J.H.; Wang, Y.Z.; Hu, S.S. Nanocomposites of graphene and graphene oxides: Synthesis, molecular functionalization and application in electrochemical sensors and biosensors. A review. Microchim. Acta 2017, 184, 1–44. [Google Scholar] [CrossRef]
  17. Quintana, M.; Spyrou, K.; Grzelczak, M.; Browne, W.R.; Rudolf, P.; Prato, M. Functionalization of graphene via 1,3-dipolar cycloaddition. ACS Nano 2010, 4, 3527–3533. [Google Scholar] [CrossRef] [PubMed]
  18. Fang, Y.; Wang, E. Electrochemical biosensors on platforms of graphene. Chem. Commun. (Camb.) 2013, 49, 9526–9539. [Google Scholar] [CrossRef]
  19. Kuila, T.; Bose, S.; Mishra, A.K.; Khanra, P.; Kim, N.H.; Lee, J.H. Chemical functionalization of graphene and its applications. Prog. Mater. Sci. 2012, 57, 1061–1105. [Google Scholar] [CrossRef]
  20. Srivastava, R.K.; Srivastava, S.; Narayanan, T.N.; Mahlotra, B.D.; Vajtai, R.; Ajayan, P.M.; Srivastava, A. Functionalized multilayered graphene platform for urea sensor. ACS Nano 2012, 6, 168–175. [Google Scholar] [CrossRef]
  21. Novoselov, K.S.; Fal’ko, V.I.; Colombo, L.; Gellert, P.R.; Schwab, M.G.; Kim, K. A roadmap for graphene. Nature 2012, 490, 192–200. [Google Scholar] [CrossRef] [PubMed]
  22. Bonaccorso, F.; Lombardo, A.; Hasan, T.; Sun, Z.P.; Colombo, L.; Ferrari, A.C. Production and processing of graphene and 2d crystals. Mater. Today 2012, 15, 564–589. [Google Scholar] [CrossRef]
  23. Wang, X.-Y.; Narita, A.; Müllen, K. Precision synthesis versus bulk-scale fabrication of graphenes. Nat. Rev. Chem. 2017, 2, 0100. [Google Scholar] [CrossRef]
  24. Novoselov, K.S.; Geim, A.K.; Morozov, S.V.; Jiang, D.; Katsnelson, M.I.; Grigorieva, I.V.; Dubonos, S.V.; Firsov, A.A. Two-dimensional gas of massless Dirac fermions in graphene. Nature 2005, 438, 197–200. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  25. Novoselov, K.S.; Geim, A.K.; Morozov, S.V.; Jiang, D.; Zhang, Y.; Dubonos, S.V.; Grigorieva, I.V.; Firsov, A.A. Electric field effect in atomically thin carbon films. Science 2004, 306, 666–669. [Google Scholar] [CrossRef] [PubMed]
  26. Zhang, Y.; Tan, Y.W.; Stormer, H.L.; Kim, P. Experimental observation of the quantum Hall effect and Berry’s phase in graphene. Nature 2005, 438, 201–204. [Google Scholar] [CrossRef] [PubMed]
  27. Novoselov, K.S.; Jiang, Z.; Zhang, Y.; Morozov, S.V.; Stormer, H.L.; Zeitler, U.; Maan, J.C.; Boebinger, G.S.; Kim, P.; Geim, A.K. Room-temperature quantum Hall effect in graphene. Science 2007, 315, 1379. [Google Scholar] [CrossRef] [PubMed]
  28. Cai, M.Z.; Thorpe, D.; Adamson, D.H.; Schniepp, H.C. Methods of graphite exfoliation. J. Mater. Chem. 2012, 22, 24992–25002. [Google Scholar] [CrossRef]
  29. Ciesielski, A.; Samori, P. Graphene via sonication assisted liquid-phase exfoliation. Chem. Soc. Rev. 2014, 43, 381–398. [Google Scholar] [CrossRef]
  30. Shen, J.; He, Y.; Wu, J.; Gao, C.; Keyshar, K.; Zhang, X.; Yang, Y.; Ye, M.; Vajtai, R.; Lou, J.; et al. Liquid Phase Exfoliation of Two-Dimensional Materials by Directly Probing and Matching Surface Tension Components. Nano Lett. 2015, 15, 5449–5454. [Google Scholar] [CrossRef]
  31. He, P.; Zhou, C.; Tian, S.; Sun, J.; Yang, S.; Ding, G.; Xie, X.; Jiang, M. Urea-assisted aqueous exfoliation of graphite for obtaining high-quality graphene. Chem. Commun. (Camb.) 2015, 51, 4651–4654. [Google Scholar] [CrossRef] [PubMed]
  32. Hummers Jr, W.S.; Offeman, R.E. Preparation of graphitic oxide. J. Am. Chem. Soc. 1958, 80, 1339. [Google Scholar] [CrossRef]
  33. Ang, P.K.; Wang, S.; Bao, Q.; Thong, J.T.; Loh, K.P. High-throughput synthesis of graphene by intercalation-exfoliation of graphite oxide and study of ionic screening in graphene transistor. ACS Nano 2009, 3, 3587–3594. [Google Scholar] [CrossRef] [PubMed]
  34. Stankovich, S.; Dikin, D.A.; Dommett, G.H.; Kohlhaas, K.M.; Zimney, E.J.; Stach, E.A.; Piner, R.D.; Nguyen, S.T.; Ruoff, R.S. Graphene-based composite materials. Nature 2006, 442, 282–286. [Google Scholar] [CrossRef] [PubMed]
  35. Chen, D.; Feng, H.; Li, J. Graphene oxide: Preparation, functionalization, and electrochemical applications. Chem. Rev. 2012, 112, 6027–6053. [Google Scholar] [CrossRef] [PubMed]
  36. Bae, S.; Kim, H.; Lee, Y.; Xu, X.; Park, J.S.; Zheng, Y.; Balakrishnan, J.; Lei, T.; Kim, H.R.; Song, Y.I.; et al. Roll-to-roll production of 30-inch graphene films for transparent electrodes. Nat. Nanotechnol. 2010, 5, 574–578. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  37. Forbeaux, I.; Themlin, J.M.; Debever, J.M. Heteroepitaxial graphite on6H−SiC(0001):Interface formation through conduction-band electronic structure. Phys. Rev. B 1998, 58, 16396–16406. [Google Scholar] [CrossRef]
  38. Berger, C.; Song, Z.; Li, X.; Wu, X.; Brown, N.; Naud, C.; Mayou, D.; Li, T.; Hass, J.; Marchenkov, A.N.; et al. Electronic confinement and coherence in patterned epitaxial graphene. Science 2006, 312, 1191–1196. [Google Scholar] [CrossRef]
  39. Kim, K.S.; Zhao, Y.; Jang, H.; Lee, S.Y.; Kim, J.M.; Kim, K.S.; Ahn, J.H.; Kim, P.; Choi, J.Y.; Hong, B.H. Large-scale pattern growth of graphene films for stretchable transparent electrodes. Nature 2009, 457, 706–710. [Google Scholar] [CrossRef]
  40. Li, X.; Cai, W.; An, J.; Kim, S.; Nah, J.; Yang, D.; Piner, R.; Velamakanni, A.; Jung, I.; Tutuc, E.; et al. Large-area synthesis of high-quality and uniform graphene films on copper foils. Science 2009, 324, 1312–1314. [Google Scholar] [CrossRef]
  41. Li, X.; Cai, W.; Colombo, L.; Ruoff, R.S. Evolution of graphene growth on Ni and Cu by carbon isotope labeling. Nano Lett. 2009, 9, 4268–4272. [Google Scholar] [CrossRef] [PubMed]
  42. Gao, L.; Ren, W.; Xu, H.; Jin, L.; Wang, Z.; Ma, T.; Ma, L.P.; Zhang, Z.; Fu, Q.; Peng, L.M.; et al. Repeated growth and bubbling transfer of graphene with millimetre-size single-crystal grains using platinum. Nat. Commun. 2012, 3, 699. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  43. Banszerus, L.; Schmitz, M.; Engels, S.; Dauber, J.; Oellers, M.; Haupt, F.; Watanabe, K.; Taniguchi, T.; Beschoten, B.; Stampfer, C. Ultrahigh-mobility graphene devices from chemical vapor deposition on reusable copper. Sci. Adv. 2015, 1, e1500222. [Google Scholar] [CrossRef]
  44. Geim, A.K.; Novoselov, K.S. The rise of graphene. Nat. Mater. 2007, 6, 183–191. [Google Scholar] [CrossRef] [PubMed]
  45. Ascherio, A.; Schwarzschild, M.A. The epidemiology of Parkinson’s disease: Risk factors and prevention. Lancet Neurol. 2016, 15, 1257–1272. [Google Scholar] [CrossRef]
  46. Logroscino, G.; Piccininni, M.; Marin, B.; Nichols, E.; Abd-Allah, F.; Abdelalim, A.; Alahdab, F.; Asgedom, S.W.; Awasthi, A.; Chaiah, Y.; et al. Global, regional, and national burden of motor neuron diseases 1990-2016: A systematic analysis for the Global Burden of Disease Study 2016. Lancet Neurol. 2018, 17, 1083–1097. [Google Scholar] [CrossRef]
  47. Trounson, A.; DeWitt, N.D. Pluripotent stem cells progressing to the clinic. Nat. Rev. Mol. Cell Biol. 2016, 17, 194–200. [Google Scholar] [CrossRef] [PubMed]
  48. Fox, I.J.; Daley, G.Q.; Goldman, S.A.; Huard, J.; Kamp, T.J.; Trucco, M. Stem cell therapy. Use of differentiated pluripotent stem cells as replacement therapy for treating disease. Science 2014, 345, 1247391. [Google Scholar] [CrossRef]
  49. Connolly, B.S.; Lang, A.E. Pharmacological treatment of Parkinson disease: A review. JAMA 2014, 311, 1670–1683. [Google Scholar] [CrossRef]
  50. Choi, J.H.; Lee, J.H.; Oh, B.K.; Choi, J.W. Localized surface plasmon resonance-based label-free biosensor for highly sensitive detection of dopamine. J. Nanosci. Nanotechnol. 2014, 14, 5658–5661. [Google Scholar] [CrossRef]
  51. Lee, J.H.; Lee, T.; Choi, J.W. Nano-Biosensor for Monitoring the Neural Differentiation of Stem Cells. Nanomaterials (Basel) 2016, 6, 224. [Google Scholar] [CrossRef] [PubMed]
  52. Ping, J.; Wu, J.; Wang, Y.; Ying, Y. Simultaneous determination of ascorbic acid, dopamine and uric acid using high-performance screen-printed graphene electrode. Biosens. Bioelectron. 2012, 34, 70–76. [Google Scholar] [CrossRef] [PubMed]
  53. Fan, Z.J.; Lin, Q.Q.; Gong, P.W.; Liu, B.; Wang, J.Q.; Yang, S.R. A new enzymatic immobilization carrier based on graphene capsule for hydrogen peroxide biosensors. Electrochim. Acta 2015, 151, 186–194. [Google Scholar] [CrossRef]
  54. Panieri, E.; Gogvadze, V.; Norberg, E.; Venkatesh, R.; Orrenius, S.; Zhivotovsky, B. Reactive oxygen species generated in different compartments induce cell death, survival, or senescence. Free Radic. Biol. Med. 2013, 57, 176–187. [Google Scholar] [CrossRef] [PubMed]
  55. Wang, L.; Ye, Y.J.; Lu, X.P.; Wu, Y.; Sun, L.L.; Tan, H.L.; Xu, F.G.; Song, Y.H. Prussian blue nanocubes on nitrobenzene-functionalized reduced graphene oxide and its application for H2O2 biosensing. Electrochim. Acta 2013, 114, 223–232. [Google Scholar] [CrossRef]
  56. Yoon, J.; Lee, T.; Bapurao, G.B.; Jo, J.; Oh, B.K.; Choi, J.W. Electrochemical H2O2 biosensor composed of myoglobin on MoS2 nanoparticle-graphene oxide hybrid structure. Biosens. Bioelectron. 2017, 93, 14–20. [Google Scholar] [CrossRef] [PubMed]
  57. Song, H.; Ni, Y.; Kokot, S. A novel electrochemical biosensor based on the hemin-graphene nano-sheets and gold nano-particles hybrid film for the analysis of hydrogen peroxide. Anal. Chim. Acta 2013, 788, 24–31. [Google Scholar] [CrossRef] [PubMed]
  58. Simplicio, J. Hemin monomers in micellar sodium lauryl sulfate. A spectral and equilibrium study with cyanide. Biochemistry 1972, 11, 2525–2528. [Google Scholar] [CrossRef]
  59. Shao, Y.; Zhang, S.; Engelhard, M.H.; Li, G.; Shao, G.; Wang, Y.; Liu, J.; Aksay, I.A.; Lin, Y. Nitrogen-doped graphene and its electrochemical applications. J. Mater. Chem. 2010, 20, 7491. [Google Scholar] [CrossRef]
  60. Ensafi, A.A.; Alinajafi, H.A.; Jafari-Asl, M.; Rezaei, B.; Ghazaei, F. Cobalt ferrite nanoparticles decorated on exfoliated graphene oxide, application for amperometric determination of NADH and H2O2. Mater. Sci. Eng. C Mater. Biol. Appl. 2016, 60, 276–284. [Google Scholar] [CrossRef]
  61. Aydogdu Tig, G. Highly sensitive amperometric biosensor for determination of NADH and ethanol based on Au-Ag nanoparticles/poly(L-Cysteine)/reduced graphene oxide nanocomposite. Talanta 2017, 175, 382–389. [Google Scholar] [CrossRef] [PubMed]
  62. Han, S.; Du, T.; Jiang, H.; Wang, X. Synergistic effect of pyrroloquinoline quinone and graphene nano-interface for facile fabrication of sensitive NADH biosensor. Biosens. Bioelectron. 2017, 89 Pt 1, 422–429. [Google Scholar] [CrossRef]
  63. Balamurugan, J.; Thanh, T.D.; Kim, N.H.; Lee, J.H. Facile fabrication of FeN nanoparticles/nitrogen-doped graphene core-shell hybrid and its use as a platform for NADH detection in human blood serum. Biosens. Bioelectron. 2016, 83, 68–76. [Google Scholar] [CrossRef] [PubMed]
  64. Liu, B.; Ouyang, X.; Ding, Y.; Luo, L.; Xu, D.; Ning, Y. Electrochemical preparation of nickel and copper oxides-decorated graphene composite for simultaneous determination of dopamine, acetaminophen and tryptophan. Talanta 2016, 146, 114–121. [Google Scholar] [CrossRef] [PubMed]
  65. Li, Y.C.; Liu, J.; Liu, M.H.; Yu, F.; Zhang, L.; Tang, H.; Ye, B.C.; Lai, L.F. Fabrication of ultra-sensitive and selective dopamine electrochemical sensor based on molecularly imprinted polymer modified graphene@carbon nanotube foam. Electrochem. Commun. 2016, 64, 42–45. [Google Scholar] [CrossRef]
  66. Thanh, T.D.; Balamurugan, J.; Lee, S.H.; Kim, N.H.; Lee, J.H. Effective seed-assisted synthesis of gold nanoparticles anchored nitrogen-doped graphene for electrochemical detection of glucose and dopamine. Biosens. Bioelectron. 2016, 81, 259–267. [Google Scholar] [CrossRef]
  67. Thanh, T.D.; Balamurugan, J.; Hwang, J.Y.; Kim, N.H.; Lee, J.H. In situ synthesis of graphene-encapsulated gold nanoparticle hybrid electrodes for non-enzymatic glucose sensing. Carbon 2016, 98, 90–98. [Google Scholar] [CrossRef]
  68. Chaiyo, S.; Mehmeti, E.; Siangproh, W.; Hoang, T.L.; Nguyen, H.P.; Chailapakul, O.; Kalcher, K. Non-enzymatic electrochemical detection of glucose with a disposable paper-based sensor using a cobalt phthalocyanine-ionic liquid-graphene composite. Biosens. Bioelectron. 2018, 102, 113–120. [Google Scholar] [CrossRef]
  69. Yang, T.T.; Xu, J.K.; Lu, L.M.; Zhu, X.F.; Gao, Y.S.; Xing, H.K.; Yu, Y.F.; Ding, W.C.; Liu, Z. Copper nanoparticle/graphene oxide/single wall carbon nanotube hybrid materials as electrochemical sensing platform for nonenzymatic glucose detection. J. Electroanal. Chem. 2016, 761, 118–124. [Google Scholar] [CrossRef]
  70. Hu, Y.; Li, F.; Han, D.; Wu, T.; Zhang, Q.; Niu, L.; Bao, Y. Simple and label-free electrochemical assay for signal-on DNA hybridization directly at undecorated graphene oxide. Anal. Chim. Acta 2012, 753, 82–89. [Google Scholar] [CrossRef]
  71. Akhavan, O.; Ghaderi, E.; Rahighi, R. Toward single-DNA electrochemical biosensing by graphene nanowalls. ACS Nano 2012, 6, 2904–2916. [Google Scholar] [CrossRef] [PubMed]
  72. Tiwari, I.; Singh, M.; Pandey, C.M.; Sumana, G. Electrochemical genosensor based on graphene oxide modified iron oxide–chitosan hybrid nanocomposite for pathogen detection. Sens. Actuators B Chem. 2015, 206, 276–283. [Google Scholar] [CrossRef]
  73. Esteban-Fernandez de Avila, B.; Araque, E.; Campuzano, S.; Pedrero, M.; Dalkiran, B.; Barderas, R.; Villalonga, R.; Kilic, E.; Pingarron, J.M. Dual functional graphene derivative-based electrochemical platforms for detection of the TP53 gene with single nucleotide polymorphism selectivity in biological samples. Anal. Chem. 2015, 87, 2290–2298. [Google Scholar] [CrossRef] [PubMed]
  74. Freedman, K.J.; Ahn, C.W.; Kim, M.J. Detection of long and short DNA using nanopores with graphitic polyhedral edges. ACS Nano 2013, 7, 5008–5016. [Google Scholar] [CrossRef] [PubMed]
  75. Wen, W.; Huang, J.Y.; Bao, T.; Zhou, J.; Xia, H.X.; Zhang, X.H.; Wang, S.F.; Zhao, Y.D. Increased electrocatalyzed performance through hairpin oligonucleotide aptamer-functionalized gold nanorods labels and graphene-streptavidin nanomatrix: Highly selective and sensitive electrochemical biosensor of carcinoembryonic antigen. Biosens. Bioelectron. 2016, 83, 142–148. [Google Scholar] [CrossRef] [PubMed]
  76. Natarajan, A.; Devi, K.S.; Raja, S.; Senthil Kumar, A. An Elegant Analysis of White Spot Syndrome Virus Using a Graphene Oxide/Methylene Blue based Electrochemical Immunosensor Platform. Sci. Rep. 2017, 7, 46169. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  77. Chen, M.; Hou, C.; Huo, D.; Bao, J.; Fa, H.; Shen, C. An electrochemical DNA biosensor based on nitrogen-doped graphene/Au nanoparticles for human multidrug resistance gene detection. Biosens. Bioelectron. 2016, 85, 684–691. [Google Scholar] [CrossRef] [PubMed]
  78. Teymourian, H.; Salimi, A.; Khezrian, S. Development of a New Label-free, Indicator-free Strategy toward Ultrasensitive Electrochemical DNA Biosensing Based on Fe3O4 Nanoparticles/Reduced Graphene Oxide Composite. Electroanalysis 2017, 29, 409–414. [Google Scholar] [CrossRef]
  79. Shahrokhian, S.; Salimian, R. Ultrasensitive detection of cancer biomarkers using conducting polymer/electrochemically reduced graphene oxide-based biosensor: Application toward BRCA1 sensing. Sens. Actuators B-Chem. 2018, 266, 160–169. [Google Scholar] [CrossRef]
  80. Huang, J.Y.; Zhao, L.; Lei, W.; Wen, W.; Wang, Y.J.; Bao, T.; Xiong, H.Y.; Zhang, X.H.; Wang, S.F. A high-sensitivity electrochemical aptasensor of carcinoembryonic antigen based on graphene quantum dots-ionic liquid-nafion nanomatrix and DNAzyme-assisted signal amplification strategy. Biosens. Bioelectron. 2018, 99, 28–33. [Google Scholar] [CrossRef]
  81. Ge, L.; Wang, W.; Sun, X.; Hou, T.; Li, F. Affinity-Mediated Homogeneous Electrochemical Aptasensor on a Graphene Platform for Ultrasensitive Biomolecule Detection via Exonuclease-Assisted Target-Analog Recycling Amplification. Anal. Chem. 2016, 88, 2212–2219. [Google Scholar] [CrossRef] [PubMed]
  82. Pandey, C.M.; Tiwari, I.; Singh, V.N.; Sood, K.N.; Sumana, G.; Malhotra, B.D. Highly sensitive electrochemical immunosensor based on graphene-wrapped copper oxide-cysteine hierarchical structure for detection of pathogenic bacteria. Sens. Actuators B-Chem. 2017, 238, 1060–1069. [Google Scholar] [CrossRef]
  83. Jijie, R.; Kahlouche, K.; Barras, A.; Yamakawa, N.; Bouckaert, J.; Gharbi, T.; Szunerits, S.; Boukherroub, R. Reduced graphene oxide/polyethylenimine based immunosensor for the selective and sensitive electrochemical detection of uropathogenic Escherichia coli. Sens. Actuators B-Chem. 2018, 260, 255–263. [Google Scholar] [CrossRef]
  84. Wu, P.; Qian, Y.D.; Du, P.; Zhang, H.; Cai, C.X. Facile synthesis of nitrogen-doped graphene for measuring the releasing process of hydrogen peroxide from living cells. J. Mater. Chem. 2012, 22, 6402–6412. [Google Scholar] [CrossRef]
  85. Sun, Y.; Luo, M.; Meng, X.; Xiang, J.; Wang, L.; Ren, Q.; Guo, S. Graphene/Intermetallic PtPb Nanoplates Composites for Boosting Electrochemical Detection of H2O2 Released from Cells. Anal. Chem. 2017, 89, 3761–3767. [Google Scholar] [CrossRef] [PubMed]
  86. Zhang, Y.; Xiao, J.; Lv, Q.; Wang, L.; Dong, X.; Asif, M.; Ren, J.; He, W.; Sun, Y.; Xiao, F.; et al. In Situ Electrochemical Sensing and Real-Time Monitoring Live Cells Based on Freestanding Nanohybrid Paper Electrode Assembled from 3D Functionalized Graphene Framework. ACS Appl. Mater. Interfaces 2017, 9, 38201–38210. [Google Scholar] [CrossRef] [PubMed]
  87. Liu, Y.; Liu, X.; Guo, Z.; Hu, Z.; Xue, Z.; Lu, X. Horseradish peroxidase supported on porous graphene as a novel sensing platform for detection of hydrogen peroxide in living cells sensitively. Biosens. Bioelectron. 2017, 87, 101–107. [Google Scholar] [CrossRef]
  88. Li, J.; Xie, J.; Gao, L.; Li, C.M. Au nanoparticles-3D graphene hydrogel nanocomposite to boost synergistically in situ detection sensitivity toward cell-released nitric oxide. ACS Appl. Mater. Interfaces 2015, 7, 2726–2734. [Google Scholar] [CrossRef]
  89. Lee, J.H.; Choi, H.K.; Yang, L.; Chueng, S.D.; Choi, J.W.; Lee, K.B. Nondestructive Real-Time Monitoring of Enhanced Stem Cell Differentiation Using a Graphene-Au Hybrid Nanoelectrode Array. Adv. Mater. 2018, 30, e1802762. [Google Scholar] [CrossRef]
  90. Rathnam, C.; Chueng, S.D.; Yang, L.; Lee, K.B. Advanced Gene Manipulation Methods for Stem Cell Theranostics. Theranostics 2017, 7, 2775–2793. [Google Scholar] [CrossRef] [Green Version]
  91. Zhang, Y.; Bai, X.; Wang, X.; Shiu, K.K.; Zhu, Y.; Jiang, H. Highly sensitive graphene-Pt nanocomposites amperometric biosensor and its application in living cell H2O2 detection. Anal. Chem. 2014, 86, 9459–9465. [Google Scholar] [CrossRef] [PubMed]
  92. Dou, B.; Li, J.; Jiang, B.; Yuan, R.; Xiang, Y. DNA-Templated In Situ Synthesis of Highly Dispersed AuNPs on Nitrogen-Doped Graphene for Real-Time Electrochemical Monitoring of Nitric Oxide Released from Live Cancer Cells. Anal. Chem. 2019, 91, 2273–2278. [Google Scholar] [CrossRef] [PubMed]
  93. Xu, H.; Liao, C.; Liu, Y.; Ye, B.C.; Liu, B. Iron Phthalocyanine Decorated Nitrogen-Doped Graphene Biosensing Platform for Real-Time Detection of Nitric Oxide Released from Living Cells. Anal. Chem. 2018, 90, 4438–4444. [Google Scholar] [CrossRef] [PubMed]
  94. Hu, X.B.; Liu, Y.L.; Wang, W.J.; Zhang, H.W.; Qin, Y.; Guo, S.; Zhang, X.W.; Fu, L.; Huang, W.H. Biomimetic Graphene-Based 3D Scaffold for Long-Term Cell Culture and Real-Time Electrochemical Monitoring. Anal. Chem. 2018, 90, 1136–1141. [Google Scholar] [CrossRef] [PubMed]
  95. Zan, X.; Bai, H.; Wang, C.; Zhao, F.; Duan, H. Graphene Paper Decorated with a 2D Array of Dendritic Platinum Nanoparticles for Ultrasensitive Electrochemical Detection of Dopamine Secreted by Live Cells. Chemistry 2016, 22, 5204–5210. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  96. Asif, M.; Aziz, A.; Wang, H.; Wang, Z.; Wang, W.; Ajmal, M.; Xiao, F.; Chen, X.; Liu, H. Superlattice stacking by hybridizing layered double hydroxide nanosheets with layers of reduced graphene oxide for electrochemical simultaneous determination of dopamine, uric acid and ascorbic acid. Mikrochim. Acta 2019, 186, 61. [Google Scholar] [CrossRef] [PubMed]
  97. Qi, M.; Zhang, Y.; Cao, C.; Zhang, M.; Liu, S.; Liu, G. Decoration of Reduced Graphene Oxide Nanosheets with Aryldiazonium Salts and Gold Nanoparticles toward a Label-Free Amperometric Immunosensor for Detecting Cytokine Tumor Necrosis Factor-alpha in Live Cells. Anal. Chem. 2016, 88, 9614–9621. [Google Scholar] [CrossRef] [PubMed]
Nanomaterials 09 00297 g001 550
Figure 1. Schematic illustration of the graphene and its derivatives synthesis methods. (a) Mechanical exfoliation (b) liquid phase exfoliation, (c) thermal decomposition, and (d) chemical vapor deposition. Reproduced with permission from [23], Copyright Springer Nature, 2017.
Figure 1. Schematic illustration of the graphene and its derivatives synthesis methods. (a) Mechanical exfoliation (b) liquid phase exfoliation, (c) thermal decomposition, and (d) chemical vapor deposition. Reproduced with permission from [23], Copyright Springer Nature, 2017.
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Figure 2. (a) Schematic of graphene oxide encapsulated molybdenum disulfide (MoS2) nanoparticle preparation for the fabrication of electrochemical biosensors composed of myoglobin (Mb) and (b) its application to H2O2 detection with improved electrochemical performance. (c–d) Amperometric response curves obtained from Mb/GO@MoS2 upon successive (c) addition of 100 nM L-ascorbic acid (AA), 100 nM sodium nitrite (NaNO2), 100 nM sodium bicarbonate (NaHCO3), and 100 nM H2O2 solutions; and by (d) addition of 100, 50, 20, and 10 nM H2O2 solutions. Reproduced with permission from [56], Copyright Elsevier, 2017.
Figure 2. (a) Schematic of graphene oxide encapsulated molybdenum disulfide (MoS2) nanoparticle preparation for the fabrication of electrochemical biosensors composed of myoglobin (Mb) and (b) its application to H2O2 detection with improved electrochemical performance. (c–d) Amperometric response curves obtained from Mb/GO@MoS2 upon successive (c) addition of 100 nM L-ascorbic acid (AA), 100 nM sodium nitrite (NaNO2), 100 nM sodium bicarbonate (NaHCO3), and 100 nM H2O2 solutions; and by (d) addition of 100, 50, 20, and 10 nM H2O2 solutions. Reproduced with permission from [56], Copyright Elsevier, 2017.
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Figure 3. (a) Scanning electron microscopy images of the graphene oxide nanowalls deposited on a graphite rod by using electrophoretic deposition (b,c) Differential pulse voltammetric profiles of the reduced graphene nanowalls (RGNW), graphene oxide nanowalls (GONW), reduced graphene nanosheet (RGNS), and graphene oxide nanosheet (GONS) electrodes as compared to the graphite and glassy carbon (GC) electrodes for detection of (b) the four free bases of DNA (G, A, T, and C) separately, and (c) equimolar mixture of G, A, T, and C. Reproduced with permission from [71], Copyright American Chemical Society, 2012.
Figure 3. (a) Scanning electron microscopy images of the graphene oxide nanowalls deposited on a graphite rod by using electrophoretic deposition (b,c) Differential pulse voltammetric profiles of the reduced graphene nanowalls (RGNW), graphene oxide nanowalls (GONW), reduced graphene nanosheet (RGNS), and graphene oxide nanosheet (GONS) electrodes as compared to the graphite and glassy carbon (GC) electrodes for detection of (b) the four free bases of DNA (G, A, T, and C) separately, and (c) equimolar mixture of G, A, T, and C. Reproduced with permission from [71], Copyright American Chemical Society, 2012.
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Figure 4. (a) Transmission electron microscopy image of the shrunked nanopore with graphite polyhedral crystal (GPC)-edges sculpted by the irradiation of electron beam (6.2 × 105 electrons/nm2·s) on to the single layer graphene. Scale bar = 5 nm. (b) Current versus voltage plot for a 5 nm nanopore (in diameter) having GPC-edges in the 2M KCl condition. (c) Current drop translocation time scatter plot for double stranded λ-DNA (5 nM concentration, 48.5 kb long) using a 5 nm nanopore with a GPC-edges at 250 mV [1 M KCl, 10 mM Tris, 1 M ethylenediaminetetraacetic acid (EDTA)]. The mean current drop value was 332 ± 62 pA by the scatter plot. (d–e) Ionic current traces for single-stranded DNA (25 bases in length) in (d) a 5 nm silicon nitride nanopores (50 nm thick) and (e) 5 nm graphene nanopores with GPC-edges. (f–h) Detailed characterization of 5 nm graphene nanopores with GPC-edges for 25 nucleotide-long DNA fragment sensing. (f) The linear increase in current drop based on applied voltages, (g) The exponential decrease of peak translocation time based on applied voltages, and (h) Calculated translocation velocity from (e). The velocity of the 25 nucleotide-long DNA fragment in 5 nm graphene nanopores with GPC-edges was 0.35 Å/μs at 100 mV/room temperature, which demonstrates the slower velocity than the silicon nitride pores. Events were recorded at 100, 125, 150, 175, 200, and 250 mV in 2 M KCl, 10 mM Tris (pH 8), 1 mM EDTA and 10 nM DNA at room temperature. Reproduced from Reference [74] with permission from the American Chemical Society.
Figure 4. (a) Transmission electron microscopy image of the shrunked nanopore with graphite polyhedral crystal (GPC)-edges sculpted by the irradiation of electron beam (6.2 × 105 electrons/nm2·s) on to the single layer graphene. Scale bar = 5 nm. (b) Current versus voltage plot for a 5 nm nanopore (in diameter) having GPC-edges in the 2M KCl condition. (c) Current drop translocation time scatter plot for double stranded λ-DNA (5 nM concentration, 48.5 kb long) using a 5 nm nanopore with a GPC-edges at 250 mV [1 M KCl, 10 mM Tris, 1 M ethylenediaminetetraacetic acid (EDTA)]. The mean current drop value was 332 ± 62 pA by the scatter plot. (d–e) Ionic current traces for single-stranded DNA (25 bases in length) in (d) a 5 nm silicon nitride nanopores (50 nm thick) and (e) 5 nm graphene nanopores with GPC-edges. (f–h) Detailed characterization of 5 nm graphene nanopores with GPC-edges for 25 nucleotide-long DNA fragment sensing. (f) The linear increase in current drop based on applied voltages, (g) The exponential decrease of peak translocation time based on applied voltages, and (h) Calculated translocation velocity from (e). The velocity of the 25 nucleotide-long DNA fragment in 5 nm graphene nanopores with GPC-edges was 0.35 Å/μs at 100 mV/room temperature, which demonstrates the slower velocity than the silicon nitride pores. Events were recorded at 100, 125, 150, 175, 200, and 250 mV in 2 M KCl, 10 mM Tris (pH 8), 1 mM EDTA and 10 nM DNA at room temperature. Reproduced from Reference [74] with permission from the American Chemical Society.
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Figure 5. Schematic illustration for the development of electrochemical white spot syndrome virus immunosensor using a methylene blue dye (MB) immobilized graphene oxide modified glassy carbon electrode. Reproduced with permission from [76], Copyright Springer Nature, 2017.
Figure 5. Schematic illustration for the development of electrochemical white spot syndrome virus immunosensor using a methylene blue dye (MB) immobilized graphene oxide modified glassy carbon electrode. Reproduced with permission from [76], Copyright Springer Nature, 2017.
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Figure 6. (a) Transmission electron microscopy image of PtPb nanoplates. (b) Chronoamperometric curves of the graphene/Intermetallic PtPb nanoplates composite (PtPb/G) electrode with the successive addition of 0.1 mM H2O2, 1 mM Uric Acid (UA), 1 mM L-Cysteine, 1 mM ascorbic acid (AA), and 1 mM glucose at a constant potential at −0.2 V. (c) Amperometric responses of the PtPb/G electrode to the addition of N-formyl methionyl-leucyl-phenylalanine (fMLP) with and without Raw 264.7 cells. Reproduced with permission from [85], Copyright American Chemical Society, 2017.
Figure 6. (a) Transmission electron microscopy image of PtPb nanoplates. (b) Chronoamperometric curves of the graphene/Intermetallic PtPb nanoplates composite (PtPb/G) electrode with the successive addition of 0.1 mM H2O2, 1 mM Uric Acid (UA), 1 mM L-Cysteine, 1 mM ascorbic acid (AA), and 1 mM glucose at a constant potential at −0.2 V. (c) Amperometric responses of the PtPb/G electrode to the addition of N-formyl methionyl-leucyl-phenylalanine (fMLP) with and without Raw 264.7 cells. Reproduced with permission from [85], Copyright American Chemical Society, 2017.
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Figure 7. (a) Schematic illustration of alkaline phosphatase (ALP) based enzymatic reaction and electrochemical sensing mechanism on the 3D surface in graphene-Au hybrid nanoelectrode arrays (NEAs) compared to 2D flat ITO surface. (b) Improved voltammetric response of graphene-Au NEAs compares to bare ITO substrate, rGO-coated ITO substrate, and Au NEAs. (c,d) Cyclic voltammogram and Anodic peak (oxidation potential: IPA) value of P-aminophenyl phosphate (PAPP) on graphene-Au NEAs before and after enzyme reaction with ALP. (e) The linear correlations between concentrations of ALP and the current signal at IPA. (f) Schematic illustration of electrochemical signal change between undifferentiated hMSCs and differentiated osteocyte based on ALP generation. (g) Cyclic voltammetry, and (h) calculated IPC values from time-dependent monitoring during osteogenesis of hMSCs (range from D1 to D21). Reproduced with permission from [89], Copyright John Wiley and Sons.
Figure 7. (a) Schematic illustration of alkaline phosphatase (ALP) based enzymatic reaction and electrochemical sensing mechanism on the 3D surface in graphene-Au hybrid nanoelectrode arrays (NEAs) compared to 2D flat ITO surface. (b) Improved voltammetric response of graphene-Au NEAs compares to bare ITO substrate, rGO-coated ITO substrate, and Au NEAs. (c,d) Cyclic voltammogram and Anodic peak (oxidation potential: IPA) value of P-aminophenyl phosphate (PAPP) on graphene-Au NEAs before and after enzyme reaction with ALP. (e) The linear correlations between concentrations of ALP and the current signal at IPA. (f) Schematic illustration of electrochemical signal change between undifferentiated hMSCs and differentiated osteocyte based on ALP generation. (g) Cyclic voltammetry, and (h) calculated IPC values from time-dependent monitoring during osteogenesis of hMSCs (range from D1 to D21). Reproduced with permission from [89], Copyright John Wiley and Sons.
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Table
Table 1. Physicochemical properties of graphene and its derivatives.
Table 1. Physicochemical properties of graphene and its derivatives.
Physicochemical PropertyEstimated ValueRef.
High surface area~2630 m2g−1[1]
Excellent electrical conductivity~1738 siemens/m[2]
Strong mechanical strengthYoung’ Modulus ~1100 GPa,
Fracture strength ~125 GPa		[3]
Thermal conductivity5000 Wm−1K−1[4]
Ease of functionalizationπ–π stacking interaction
Electrostatic interaction		[5]
Table
Table 2. Comparison of different graphene-based electrode for small molecule detection.
Table 2. Comparison of different graphene-based electrode for small molecule detection.
Electrode MaterialsTargetLinear RangeDetection LimitRef.
Graphene capsule/horseradish peroxidase H2O20.01–12 mM3.3 μM[53]
Prussian blue nanocubes/nitrobenzene/reduced graphene oxideH2O21.2 μM–15.25 mM0.4 μM[55]
Myoglobin (Mb)/MoS2 nanoparticle/graphene oxideH2O2-20 nM[56]
Hemin porphyrin/graphene/gold nanoparticleH2O20.3 μM–1.8 mM0.11 μM[57]
Cobalt ferrite nanoparticles decorated exfoliated graphene oxideH2O2
NADH		0.9–900 μM
0.50–100 μM		0.54 μM
0.38 μM		[60]
Au-Ag nanoparticles/poly(L-Cysteine)/reduced graphene oxideNADH
ethanol		0.083 µM–1.05 mM
0.017 µM–1.845 mM		9.0 nM
5.0 µM		[61]
Graphene-pyrroloquinoline quinone NADH
0.32 µM–220 µM0.16 µM[62]
FeN nanoparticles/nitrogen-doped graphene core-shell NADH0.4 µM–718 μM25 nM[63]
Screen-printed grapheneDopamine
Ascorbic acid
Uric acid		0.5 µM–2000 μM
4.0 µM–4500 μM
0.8 µM–2500 μM		0.12 μM
0.95 μM
0.20 μM		[52]
Nickel and copper oxides-decorated grapheneDopamine0.5 µM–20 μM0.17 μM[64]
Molecularly imprinted polymer modified graphene/carbon nanotube Dopamine2.0 fM–1.0 pM667 aM[65]
Gold nanoparticle-anchored nitrogen-doped graphene Dopamine
glucose		30 nM–48 μM
40 μM–16.1 mM 		10 nM
12 μM		[66]
Graphene-encapsulated gold nanoparticle glucose6 μM–28.5 mM 1 μM[67]
Cobalt phthalocyanine–ionic liquid–graphene glucose0.01–1.3 mM
and 1.3–5.0 mM 		0.67 µM
 		[68]
Copper nanoparticle/graphene oxide/single wall carbon nanotube glucose1 μM–4.538 mM0.34 μM[69]
Table
Table 3. Comparison of different graphene-based electrode for protein/nucleic acid detection.
Table 3. Comparison of different graphene-based electrode for protein/nucleic acid detection.
Electrode MaterialsTargetLinear RangeDetection LimitRef.
Graphene Oxide/probe DNAHIV-1 gene (cDNA)1 pM–1 μM0.11 pM[70]
Reduced graphene nanowallsdsDNA0.1 fM–10 mM9.4 zM[71]
Graphene oxide modified iron
Oxide/chitosan/probe DNA		Escherichia coli Ο157:¨Η7 gene (cDNA)10 fM–1 μM10 fM[72]
Screen-printed carbon/reduced graphene oxide/Carboxy-methyl-cellulose/probe DNAp53 tumor suppressor gene
(cDNA)		10 nM–0.1 μM2.9 nM[73]
Nitrogen-doped graphene/Au nanoparticles/probe DNAmultidrug resistance gene10 fM–100 nM3.12 fM[77]
Fe3O4 Nanoparticles/reduced graphene oxideHIV-1 gene
(cDNA)		10 aM–100 pM-[78]
Glassy carbon/reduced graphene oxide/polypyrrole–3–carboxylic acidBreast cancer 1 gene1 pM–0.1 μM0.3 pM[79]
Gold nanorods/graphene/ hairpin-shaped DNA aptamer Carcinoembryonic antigen 5 pg·mL−1–50 ng·mL−11.5 pg·mL−1[75]
Graphene quantum dot-ionic liquid-nafion/hairpin aptamerCarcinoembryonic antigen0.5 fg·mL−1–0.5 ng mL−10.34 fg·mL−1[80]
Graphene/glassy carbon/aptamerCarcinoembryonic antigen80 ag·mL−1–950 fg·mL−180 ag·mL−1[81]
Glassy carbon/graphene oxide methylene blue/AntibodyWhite spot syndrome virus1.36 × 10−3–107 copies·µL−1103 copies·µL−1[76]
Graphene-wrapped copper oxide/cysteineE. coli O157:H710 CFU·mL−1–108 CFU·mL−13.8 CFU·mL−1[82]
Gold/reduced graphene oxide/polyethylenimine E. coli10 CFU·mL−1–104 CFU·mL−110 CFU·mL−1[83]
Table
Table 4. Comparison of different graphene-based electrode for live cell-based detection.
Table 4. Comparison of different graphene-based electrode for live cell-based detection.
Electrode MaterialsTargetLinear RangeDetection LimitRef.
Nitrogen doped grapheneH2O20.5 μM–1.2 mM0.05 μM[84]
Graphene/PtPb-nanoplate H2O22 nM–2516 μM2 nM[85]
Gold nanoflowers modified ionic liquid functionalized graphene frameworkH2O20.5 μM–2.3 mM100 nM[86]
HRP supported Porous grapheneH2O22.77 μM –835 μM26.7 pM[87]
Graphene-Pt nanocomposites H2O20.5 μM–0.475 mM0.2 μM[91]
GNP deposited 3D graphene hydrogelNO200 nM –6 μM9 nM[88]
GNP/calf thymus DNA/nitrogen-doped graphene NO2 nM–500 nM0.8 nM[92]
Iron phthalocyanine decorated nitrogen-doped graphene on ITONO0.18 μM–400 μM0.18 μM[93]
3-aminophenylboronic acid functionalized graphene foam networkH2S0.2 μM–10 μM50 nM[94]
Dendritic Pt nanoparticles decorated freestanding graphene paperDA87 nM–100 μM5 nM[95]
Zn-NiAl layered double hydroxide on reduced graphene oxideDA1 nM–1 μM0.1 nM[96]
Aryldiazonium Salts and GNP decorated reduced graphene oxide TNF-α0.1–150 pg·mL−10.1 pg·mL−1[97]
Graphene-Au hybrid nanoelectrode arrayALP0.1–10 unit·mL−10.03 unit·mL−1[89]

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Lee, J.-H.; Park, S.-J.; Choi, J.-W. Electrical Property of Graphene and Its Application to Electrochemical Biosensing. Nanomaterials 2019, 9, 297. https://doi.org/10.3390/nano9020297

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Lee J-H, Park S-J, Choi J-W. Electrical Property of Graphene and Its Application to Electrochemical Biosensing. Nanomaterials. 2019; 9(2):297. https://doi.org/10.3390/nano9020297

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Lee, Jin-Ho, Soo-Jeong Park, and Jeong-Woo Choi. 2019. "Electrical Property of Graphene and Its Application to Electrochemical Biosensing" Nanomaterials 9, no. 2: 297. https://doi.org/10.3390/nano9020297

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