Baicalin Alleviate Apoptosis via PKC-MAPK Pathway in Porcine Peritoneal Mesothelial Cells Induced by Glaesserella parasuis
Abstract
:1. Introduction
2. Results
2.1. Baicalin Inhibits GPS Induced Apoptosis
2.2. Baicalin Inhibits the Expression of Apoptosis-Related Genes Induced by GPS
2.3. Baicalin Inhibits GPS Induced Apoptosis via PKC-MAPK Pathway
3. Discussion
4. Materials and Methods
4.1. Drugs
4.2. Bacterial Strains
4.3. Cell Culture
4.4. Cell Apoptosis
4.5. RNA Isolation and Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR)
4.6. Western Blot
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Nucleotide Sequences (5’–3’) | Tm (°C) | Length (bp) | |
---|---|---|---|---|
β-actin | Forward | TGCGGGACATCAAGGAGAAG | 57.4 | 216 |
Reverse | AGTTGAAGGTGGTCTCGTGG | 57.4 | ||
Bax | Forward | GCCGAAATGTTTGCTGACG | 55.2 | 156 |
Reverse | GAGCCGATCTCGAAGGAAGT | 57.4 | ||
C-myc | Forward | GGTCTTCCCCTACCCACT | 57.2 | 200 |
Reverse | CCTCATCCTCTTGTTCTTCC | 55.4 | ||
Bcl-xl | Forward | GCAACCCATCCTGGCACCT | 59.5 | 136 |
Reverse | TCAAACTCATCGCCCGCCT | 57.3 | ||
c-fos | Forward | GCTGACAGATACACTCCAAGCGG | 61.3 | 542 |
Reverse | AGGAAGACGTGTAAGTAGTGCAG | 57.8 | ||
c-jun | Forward | CGCCAGTCTACGCTAATC | 55.1 | 288 |
Reverse | GGTTCCTCATACGCTTCC | 54.8 |
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Lu, Q.; Zhou, L.; Wang, Z.; Li, X.; Ding, L.; Qiu, Y.; Guo, P.; Ye, C.; Fu, S.; Wu, Z.; et al. Baicalin Alleviate Apoptosis via PKC-MAPK Pathway in Porcine Peritoneal Mesothelial Cells Induced by Glaesserella parasuis. Molecules 2022, 27, 5083. https://doi.org/10.3390/molecules27165083
Lu Q, Zhou L, Wang Z, Li X, Ding L, Qiu Y, Guo P, Ye C, Fu S, Wu Z, et al. Baicalin Alleviate Apoptosis via PKC-MAPK Pathway in Porcine Peritoneal Mesothelial Cells Induced by Glaesserella parasuis. Molecules. 2022; 27(16):5083. https://doi.org/10.3390/molecules27165083
Chicago/Turabian StyleLu, Qirong, Lang Zhou, Ziyue Wang, Xiaomin Li, Li Ding, Yinsheng Qiu, Pu Guo, Chun Ye, Shulin Fu, Zhongyuan Wu, and et al. 2022. "Baicalin Alleviate Apoptosis via PKC-MAPK Pathway in Porcine Peritoneal Mesothelial Cells Induced by Glaesserella parasuis" Molecules 27, no. 16: 5083. https://doi.org/10.3390/molecules27165083