Next Article in Journal
A Study on the Direct Esterification of Monoalkylphosphates and Dialkylphosphates; The Conversion of the Latter Species to Trialkylphosphates by Alkylating Esterification
Previous Article in Journal
Pt-Chitosan-TiO2 for Efficient Photocatalytic Hydrogen Evolution via Ligand-to-Metal Charge Transfer Mechanism under Visible Light
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 27
Issue 15
10.3390/molecules27154671
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

The Impact of Soil pH on Heavy Metals Uptake and Photosynthesis Efficiency in Melissa officinalis, Taraxacum officinalis, Ocimum basilicum

by
Dorota Adamczyk-Szabela
* and
Wojciech M. Wolf
Institute of General and Ecological Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland
*
Author to whom correspondence should be addressed.
Molecules 2022, 27(15), 4671; https://doi.org/10.3390/molecules27154671
Submission received: 17 May 2022 / Revised: 18 July 2022 / Accepted: 19 July 2022 / Published: 22 July 2022
Browse Figures
Review Reports Versions Notes

Abstract

:
Herbs used in medicine should be grown under controlled and standardized conditions. Agricultural practices often induce changes to soil pH, which may affect migration of heavy metals in the environment, their accumulation in plant tissues and the concentration of medicinal ingredients. The aim of this work was to assess the influence of various soil pH on the biological parameters and uptake of manganese, copper and zinc by basil, dandelion and lemon balm. The soil analysis covered pH, organic matter content, bioavailable and total forms of investigated metals in soil. In plants cultivated in soil at pH covering the range 4.7–8.5 the concentrations of Mn, Cu and Zn were analyzed. Their mobility and availability were assessed by bioaccumulation factors, translocation factors and transfer coefficients. The seed germination and subsequent herbs growth were strongly dependent on soil pH for all investigated plant species. Photosynthetic efficiency at different pHs was positively correlated with uptake of Cu and Mn while Zn behaved in a more random way.

1. Introduction

Soil pH affects almost all biological and chemical processes which control terrestrial ecosystems. Notably, it alters plant metabolism and biomass yield [1]. In particular, the soil pH significantly influences the solubility, bioavailability and translocation of heavy metals in plant species [2]. The latter interaction has been well documented in numerous studies [3]. In general, the majority of micronutrients are better available to plants cultivated in acidic soils than in neutral or alkaline conditions [4]. On the other hand, soil pH mediates metal toxicity [5]. At high pH, metals tend to form barely soluble phosphates and carbonates. On the contrary, at low pH they are likely to exist in more bioavailable free ionic forms [6]. Their migration is to a large extent mediated by Coulombic forces [7]. In particular, uptake of metal cations from the rhizosphere by the root usually results in the discharge of equivalent number of protons [1].
In these conditions metal cations compete with protons embedded in biological matrices and are involved in diverse interactions [8]. On the other hand, numerous studies emphasize that plants can be significantly affected by the biologically available forms of metals in soil only. The remaining fraction is sequestered or irreversibly bounded to the soil matrix and usually does not interact directly with plants. Notable, both forms of metals can be transformed one into another by changes of the soil pH [9].
Zinc, manganese and copper are essential for plants at low levels and are involved in a number of biochemical reactions. In particular, zinc is a cofactor located in an active site of chloroplastic b carbonic anhydrase (b-CA), which catalyzes rapid, reversible interconversion of HCO3− and CO2 and supplies RuBisCo with CO2 [10]. Additionally, Zn-finger proteins are engaged in photosynthesis where they affect transcription through the site-specific interactions [11].
Manganese is involved in a number of processes which govern plant growth and development. In particular, it initiates photosynthesis by the water-splitting reaction in the photosystem II. It is catalyzed by the cluster Mn4O5Ca, which supports partition of two water molecules into four electrons, four protons, and molecular O2 [12,13].
Copper is a key component of the electron transport chain in photosystem I and acts as a redox center in plastocyanin macromolecule [14,15]. It is also involved in the activity of Cu/Zn-superoxide dismutase (Cu/Zn-SOD), a protein which is localized in the stroma of chloroplasts and is involved in the scavenging of reactive oxygen species [10].
Herbs and spice plants are particularly susceptible to changes in soil, which substantially affects their constituency and properties. Therefore, careful adjustment of growing conditions is crucial as far as medicinal value of herbs is concerned [16]. In this work we investigated three popular herbs of high medicinal value. Melissa officinalis L., (lemon balm, bee balm, honey balm) is a perennial herb belonging to the Lamiaceae (mint) family. This plant is native to Europe, Central Asia and Iran but now has been disseminated in both Americas and elsewhere [17]. Its leaves contain flavonoids, beneficial volatile compounds, triterpenes, polyphenols and essential oils. M. officinalis possesses sedative, antibacterial, antiviral, and antifungal activities. It is an easy herb to grow that can be cultivated in diverse soils and climatic conditions [18].
Taraxacum officinalis (dandelion) is a member of the Asteraceae family. It is a perennial herb, native throughout the Northern hemisphere, found growing wild in meadows, pastures and waste grounds of temperate zones. Dandelion root and leaf are used widely in Europe for gastrointestinal ailments. Due to the high content of minerals, fiber, vitamins, unsaturated fatty acids, inulin, phytosterols, amino acids [19], sesquiterpenes, triterpenes, phytosterols and phenolic compounds, dandelion is a very popular medicinal plant with valuable properties [20].
Ocimum basilicum L. (sweet basil) is cultivated in many countries of the world as an edible, medicinal and industrial plant [21]. It has antibacterial, antioxidant, antifungal and anti-inflammatory properties. Basil contains many valuable ingredients, including rosmarinic and cinnamic acid, flavonoids and anthocyanins, essential oils and saponins [22].
In order to ensure highly efficient crop production, inorganic fertilizers and pesticides are widely applied. They affect diverse components of the environment, with the accumulation of heavy metals in soil and plants being the most prone. Furthermore, herbs and spice plants are particularly susceptible to changes in soil, which substantially affects their constituency and properties. Therefore, careful adjustment of growing conditions is crucial as far as medicinal value of herbs is concerned [16]. This work follows our investigations on how soil pH controls heavy metals uptake by herbs and for the first time shows photosynthetic background of this process.

2. Results

2.1. Analysis of Soil Used in the Study

The soil used in this study was organic and acidic [23,24,25]. Total manganese, copper and zinc contents demonstrated that it was not contaminated by these metals [26,27] (Table 1a). Bioavailable forms of Mn, Cu and Zn were determined in either initial soil without additives at pH = 6.0 or modified soil samples with pH adjusted to 4.7 and 8.7, respectively (Table 1b). Acidic conditions prompted mobility of all metals, while the basic environment increased Mn and Cu levels and simultaneously decreased bioavailable content of Zn.

2.2. Effect of pH on Plant Growth and Photosynthesis

Influence of soil pH on index of chlorophyll in leaves (Chl), the activity of net photosynthesis (PN), stomatal conductance (Gs), transpiration rate (E) and intercellular concentration of CO2 (Ci) augmented with heights of all investigated plants are in Figure 1. The largest plant heights of basil and dandelion were observed for original soil at pH = 6.0 Lemon balm behaved in and odd way, the highest plants were grown at pH = 4.7. The basic environment (pH = 8.7) substantially inhibited growth of all three plants (Figure S1). Alterations in the height of basil and dandelion plants were consistent with their photosynthesis indicators while lemon balm behaved in a different way. The largest Chl was observed at pH = 6.0 for all investigated plants while the highest net photosynthesis was found in the dandelion grown in soil at pH = 6.0. The intercellular CO2 concentrations did not differ substantially for all pH values. The alkaline reaction of the soil (pH = 8.7) reduced the net photosynthesis in lemon balm by 40% as compared to the control treatment (pH = 6.0) while the acidic conditions (pH = 4.7) prompted only 36% decrease. Dandelion and basil plants reacted in a less pronounced way. The most sensitive to alkaline environment was dandelion. It reduced photosynthesis intensity by closing stomata, which further reduced transpiration and CO2 capture by the plant. Those processes help to keep the intercellular CO2 concentration at a stable level and do not affect chlorophyll content substantially. Therefore, the plant photosynthesis apparatus remains ready for increasing its efficiency upon pH returning to neutral values.

2.3. Effect of pH on Heavy Metals Contents in Plants

Metal contents in either above-ground parts or roots of basil, lemon balm and dandelion plants cultivated in pH modified soils are in Figure 2. Metals uptake was assessed by the one-way ANOVA at the 0.95 probability level (Table 2) and followed by the post hoc Tukey’s test [28]. The null hypothesis supported by those calculations was whether changes of soil pH affected migration of metals from soil to the plant.
In basil Mn, Cu and Zn accumulation in roots was higher than that in above-ground parts (Figure 2). In lemon balm and dandelion concentrations of manganese in above-ground parts were larger than those in roots while zinc was accumulated mostly in roots. Metals uptake by plants may be conveniently assessed by the bioaccumulation factor (BAF), translocation factors (TF) and transfer coefficients (TC) (Table 3). The former is defined as the ratio between the metal concentration in the above-ground parts of plant and its content in the soil. TF is the ratio between respective concentrations in above-ground parts and the plant roots, while TC represents a fraction of the metal content in roots as compared to that in the soil [29,30,31].
In all investigated plants increasing soil pH hampered Zn transport from soil to either roots or shoots. The manganese uptake was intensified in dandelion and lemon balm plants in either acidic or alkaline soils while the latter environment restricted Mn transport in basil. The copper uptake was prompted by acidic conditions in basil and almost neutral environment (pH = 6.0) in dandelion and lemon balm. Notably, migration of metals from roots to shoots as defined by respective translocation factors [32] is quite pH-independent. Soil pH has very limited influence on metals transfer within the plant tissues as indicated by the TF values. High metal mobility in the acidic soil stimulated their uptake by roots in basil and dandelion. However, the dandelion roots accumulated manganese most efficiently in the basic environment. Lemon balm prompted copper uptake by above-ground parts in the neutral and basic treatments.
In the latter plant, the only exception was manganese, which was better absorbed by roots in the basic environment. Lemon balm prompted copper uptake by above-ground parts in the neutral and basic treatments.

3. Discussion

Bioavailability of heavy metals to plants is strictly related to their mobility in soil. The latter is governed by the soil pH, which is also an important factor responsible for the metal migration in plants tissues [1]. At low pH metals are likely to exist in cationic forms, which are less prone to sorption on soil surfaces [33].
The solubility of Mn in soil solution is strongly related to pH with the lowest values in the neutral or close to neutral environments. On the other hand, acidic soils prompt the Mn solubility while anionic complexes facilitate increased Mn solubility in alkaline solutions. Formation of copper complexes strongly affects its solubility in soil. At pH below 7, copper exists as hydrolysis products (CuOH+ and Cu2(OH2)22+), while above pH = 8 it is transformed to anionic hydroxy complexes.
Zinc is the most amphoteric of all investigated metals. In acidic conditions its cations are highly mobile. At neutral pH its absorption is significantly governed by exchange within the soil cation sites. The latter effect is mediated by competing cations, which decrease Zn2+ adsorption and increase its mobility. In alkaline environments, zinc promptly reacts with organic ligands yielding complex entities with inorganic anions.
Depending on the soil character the latter mechanism may either increase or decrease zinc mobility [34].
Distinct plants enhance metals uptake at specific pH values [35]. Particular species have different root morphologies and distributions in soil profiles. They display different patterns of nutrient uptake and H+ excretion along the root axis as well as down the soil profile. Additionally, plants have the ability to buffer pH changes [36].
Root uptake is mainly facilitated by low pH in all investigated plants. Exceptions were identified for uptakes of Mn in dandelion and Cu in lemon balm. A similar situation was already observed in Valeriana officinalis L. grown in soil at very alkaline, pH = 10.0 [37]. At high pH both metals are more prone to complexation by soil organic matter, which increases their bioavailability to plants. Plants adapt to alkaline environment by secreting acidic substances from their roots to the rhizosphere [38]. This process, which facilitates nutrients uptake, is very plant-specific [39,40]. However, final yield depends on either complexation or rhizosphere acidification and may be treated as an indicator of particular plant resistance to diverse pH conditions [34].
Cell walls of the apoplast are incrusted with sulfuric and carboxylic groups, the charges of which may be easily modified by the pH alterations. In a charged form those entities bind copper cations and decrease their transport to the upper parts of the plant. On the other hand, accumulation of copper chelates in symplast may be facilitated by their substantial affinity to amino acids, peptides and proteins.
Quite the similar effect was identified by Zhao et al. 2013 [35]. They observed that morphological parameters of P. lactiflora were decreased in respect to pH = 7.0 at either pH = 4.0 or 10.0. This could be triggered by toxic effect of H+, Mn2+ and Zn2+ cations and reduced availability of Mg, Ca, K, P and Mo elements in acidic conditions. In alkaline environment, the uptake of essential metals and other trace elements was reduced [41]. Soil pH alters metal uptake mostly at the root level. Further transport to shoots is altered to a much lesser extent. Metals enter the root through either transmembrane symplastic or apoplastic pathways [42]. The latter uses space between cells. Passing through the membrane is facilitated by transporter proteins, which are specifically related to particular elements [43]. Similar mechanisms govern further transport from roots to shoots. In all investigated plants metals migration from roots to shoots was to a much lesser extent affected by the pH modifications with copper being the most prone. Zinc and manganese are usually transported by the symplastic pathways, while copper partially follows the apoplastic avenues [44]. We speculate that the latter mechanism is more affected by soil pH changes, which propagate within plant tissues through free spaces of the apoplast. Cell walls of the apoplast are incrusted with sulfuric and carboxylic groups, the charges of which may be modified by the pH alterations. In a charged form those entities bind copper cations and decrease their transport to the upper parts of the plant. On the other hand, accumulation of copper chelates in the symplast may be facilitated by their substantial affinity to amino acids, peptides and proteins [44,45].
Divalent cations are the only Mn species that are available for plants. On the contrary, the higher valence oxides, which are the products of autooxidation at alkaline pH, are not easily accessible. Manganese uptake is a biphasic pathway. Initially, cations are reversible and rapidly absorbed by the root apoplast. Further transport is mostly realized in an active way by the intercellular symplast transport. However, as pointed out by Humphries et al. [46] and Milaleo et al. [47] the explicit mechanism has not been thoroughly characterized as yet. Zinc uptake follows the symplastic pathway and is supported by transport proteins, which are located in cell membrane. It actively depends on the plant metabolism. Cell interior is isolated from the outer space by the membrane, which helps to maintain neutral pH regardless the external environment. This situation facilitates binding zinc cations to organic chelators inside the cell and significantly reduces their mobility [48].
In all investigated plants the highest rates of photosynthesis were observed at neutral pH, while the lowest were found for alkaline environment. The intercellular CO2 and stomatal conductance roughly followed that pattern but were less vulnerable upon extreme pH values. Copper uptake by the lemon balm followed the above distributions of photosynthesis parameters. Additionally, in this herb net photosynthesis, transpiration rate, stomatal conductance and intercellular CO2 were among the lowest observed in all three investigated plants. Copper and manganese migration is related to the activation of side groups in the apoplast. This effect increased retention of metals in above-ground parts of plants. Therefore, we speculate that Mn and Cu migration in dandelion and lemon balm, respectively, was realized in a passive way through empty spaces of the apoplast. On the other hand, a systematic decrease in zinc concentrations in either roots or above-ground parts upon pH increase was observed for all investigated herbs. Its concentrations in either roots or upper parts of the plants were the highest at the lowest pH level.
The photosynthesis apparatus is located inside the cell and strongly dependent upon internal pH stabilization. Hydrogen cations enter cell cytoplasm via a number of ways, with the transporting channels being the most important for pH control. Our results indicate that photosynthetic efficiency at different pH is positively correlated with uptake of passively transported metals such as Cu and Mn. The active zinc transport is less pH-dependent.

4. Materials and Methods

4.1. Soil Analysis

Soil samples were collected on agriculture farmland in Lagiewniki (51°51′ N, 19°28′ E, which was not fertilized nor subjected to pesticide treatments for at least year before the beginning of the experiment; located away from traffic) according to the PN-ISO 10381-4: 2007 [49] in August 2019. The sampled soils were dried in an air-circulating room, followed by removal of stones and plant residues, and were passed through a 2 mm stainless steel sieve.
Soil pH was measured according to PN-ISO 10390:1997 [23]. Organic matter content in soil was determined by the gravimetric method [24,25]. For analysis of bioavailable metals in soil, air-dried soils were extracted by 0.5 mol L−1 of HCl according to standard [50]. The method involves shaking 10 g soil sample with 100 mL of 0.5 mol/l HCl for 1 h at room temperature (25 °C) followed by measurement of element concentrations in the filtrate.
The mixture of concentrated HNO3 (6 mL) and HCl (2 mL) was applied during mineralization of soil (0.5000 g) with the Anton Paar Multiwave 3000 closed system instrument. Metals contents were measured in soil samples by the HR-CS FAAS with the contraAA 300 (Analytic Jena spectrometer). Five replications were conducted for each sample.

4.2. The Soil pH Adjustment

Soil pH was modified to values 4.7 and 8.7. Soil samples (300 g) were placed in plastic pots (5 for each pH value) and then 150 mL (0.5 mol L−1) H2SO4 or 60 g CaO were added to each sample and carefully mixed. Afterward, the soil samples were air-dried and subjected to pH measurements. Moreover, the bioavailable forms of manganese, copper and zinc were determined in soil samples with modified pH values.

4.3. Cultivation of Plant Material

All herbs were cultivated under laboratory conditions by the pot method [36] from March to July 2019. Seeds of Melissa Officinalis, Taraxacum officinalis, Ocimum basilicum (from P.H. Legutko company, Poland) in an amount of approximately 20 seeds per pot were placed in plastic containers with a diameter of 12 cm (45 pieces −5 for each pH value and control). The cultivation was conducted in green house under controlled conditions: the temperatures were 23 ± 2 and 16 ± 2 °C for day and night, respectively; the relative humidity was limited to 70–75%; and the photosynthetic active radiation (PAR) during the 16 h photoperiod was restricted to 400 μmol m−2 s−1. All plants were regularly watered with deionized water. After 3 months the herbs were harvested separately into above-ground material and roots. The weights of the individual parts of the plants were determined. The roots were washed in tap water and deionized water. The harvest was oven-dried at 45 °C to a constant weight, homogenized and ground.

4.4. The Morphological and Physiological Parameters of Plants

The above-ground parts lengths were determined by measurement of distance between the crown and the leaf tip (cm). Index of chlorophyll was measured using the Konica Minolta SPAD-502Plus, Tokyo, Japan. Activity of net photosynthesis (PN), stomatal conductance (Gs), intercellular concentration of CO2 (Ci) and transpiration (E) were determined with the gas analyzer apparatus CIRAS-3 (Portable Photosynthesis System, Amesbury, MA, USA) [51,52]. All measurements were made five times on separate plants without damaging the plant material.

4.5. Determination of Heavy Metals in Herbs

The concentrations of metals in roots and above-ground parts of herbs were determined in mineralizates of each plant material. The same protocol as used for soil analysis was applied.
Quality assurance and quality control (QA/QC) for metals in plants samples were estimated by determining metal contents in the certified reference material INCT-MPH-2 containing a mixture of selected Polish herbs [53] (Table S1).

5. Conclusions

In summary, soil pH alters biological parameters as well as manganese, copper and zinc uptake by basil, dandelion and lemon balm in a number of ways. This issue is particularly important for keeping medicinal value of herbal crops at a high and stable level. Investigated metals play significant role in the photosynthesis process. Its efficiency at different soil pH values positively correlates with uptake of Cu and Mn, while Zn behaved in a more unpredicted way. In all investigated plants the highest rates of photosynthesis were observed at neutral pH, while the lowest were found for alkaline environment. The intercellular CO2 and stomatal conductance were less vulnerable upon extreme pH values. The most sensitive to alkaline conditions was dandelion. It reduced photosynthesis intensity by closing stomata, which further limited transpiration and CO2 capture by the plant. Those processes help to keep intercellular CO2 concentration at stable level without affecting chlorophyll content substantially.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/molecules27154671/s1, Figure S1: Basil (a), dandelion (b) and lemon balm (c) plants are grown in different soil pH.; Table S1: Metals content in certified reference materials (p = 0.95, n = 5).

Author Contributions

Conceptualization, D.A.-S. and W.M.W.; methodology, D.A.-S.; software, D.A-S.; validation, D.A.-S.; formal analysis, D.A.-S.; investigation, D.A.-S.; resources, D.A-S. and W.M.W.; data curation D.A.-S. and W.M.W.; writing—original draft preparation, D.A.-S.; writing—review and editing, D.A.-S. and W.M.W.; visualization, D.A.-S.; supervision, D.A.-S. and W.M.W.; project administration, D.A.-S. and W.M.W.; funding acquisition, D.A.-S. and W.M.W. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by Regional Fund for Environmental Protection and Water Management in Łódz, Poland, grant numbers: 804/BNID/2016 and 58/BN/D2018. Additional funding from the Institute of General and Ecological Chemistry of Łódz University of Technology is also acknowledged.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Acknowledgments

The European University Foundation is acknowledged for advising on the legal and social dimension of this study and Jakub Kubicki for advice and help during heavy metal determinations.

Conflicts of Interest

The authors declare no conflict of interest. The founders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

Sample Availability

Not available.

References and Note

  1. Neina, D. The role of soil pH in plant nutrition and soil remediation. Appl. Environ. Soil Sci. 2019, 2019, 5794869. [Google Scholar] [CrossRef]
  2. Li, Q.; Wang, Y.; Li, Y.; Li, L.; Tang, M.; Hu, W.; Li, C.; Ai, S. Speciation of heavy metals in soils and their immobilization at microscale interfaces among diverse soil components. Sci. Total Environ. 2022, 825, 153862. [Google Scholar] [CrossRef] [PubMed]
  3. Draszawka-Bołzan, B. Effect of pH and soil environment. WNOFNS 2017, 8, 50–60. Available online: www.worldnewsnaturalsciences.com./wp-content/uploads/2012/11/WNOFNS-8-2017-50-60-1.pdf (accessed on 16 May 2022).
  4. Gentili, R.; Ambrosini, R.; Montagnani, C.; Caronni, S.; Citterio, S. Effect of soil pH on the growth, reproductive investment and pollen allergenicity of Ambrosia artemisiifolia L. Front. Plant Sci. 2018, 9, 1335. [Google Scholar] [CrossRef] [Green Version]
  5. Strawn, D.G. Sorption Mechanisms of Chemicals in Soils. Soil Syst. 2021, 5, 13. [Google Scholar] [CrossRef]
  6. Olaniran, A.O.; Balgobind, A.; Pillay, B. Bioavailability of heavy metals in soil: Impact on microbial biodegradation of organic compounds and possible improvement strategies. Int. J. Mol. Sci. 2013, 14, 10197–10228. [Google Scholar] [CrossRef] [Green Version]
  7. Kim, R.-Y.; Yoon, J.-K.; Kim, T.-S.; Yang, J.E.; Owens, G.; Kim, K.-R. Bioavailability of heavy metals in soils: Definitions and practical implementation—A critical review. Environ. Geochem. Health 2015, 37, 1041–1061. [Google Scholar] [CrossRef]
  8. Violante, A.; Cozzolino, V.; Perelomov, L.; Caporale, A.G.; Pigna, M. Mobility and bioavailability of heavy metals and metalloids in soil environments. J. Soil Sci. Plant Nutr. 2010, 10, 268–292. [Google Scholar] [CrossRef] [Green Version]
  9. Lu, H.; Li, K.; Nkoh, J.; He, X.; Xu, R.; Qian, W.; Shi, R.; Hong, Z. Effects of pH variations caused by redox reactions and pH buffering capacity on Cd(II) speciation in paddy soils during submerging draining alternation. Ecot. Environ. Saf. 2022, 234, 113409. [Google Scholar] [CrossRef]
  10. Yruela, I. Transition metals in plant photosynthesis. Metallomics 2013, 5, 1090–1109. [Google Scholar] [CrossRef] [Green Version]
  11. Han, G.; Lu, C.; Guo, J.; Qiao, Z.; Sui, N.; Qiu, N.; Wang, B. C2H2 Zinc finger proteins: Master regulators of abiotic stress responses in plants. Front. Plant Sci. 2020, 11, 115–128. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  12. Bricker, T.M.; Roose, J.L.; Fagerlund, R.D.; Frankel, L.K.; Eaton-Rye, J.J. The extrinsic proteins of Photosystem II. Biochim. Biophys. Acta Bioenerg. 2012, 1817, 121–142. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  13. Alejandro, S.; Höller, S.; Meier, B.; Peiter, E. Manganese in Plants: From acquisition to subcellular allocation. Front. Plant Sci. 2020, 11, 300–323. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  14. Yamasaki, H.; Pilon, M.; Shikanai, T. How do plants respond to copper deficiency? Plant Signal. Behav. 2008, 3, 231–232. [Google Scholar] [CrossRef] [Green Version]
  15. Aguirre, G.; Pilon, M. Copper delivery to chloroplast proteins and its regulation. Front. Plant Sci. 2016, 6, 1250–1260. [Google Scholar] [CrossRef] [Green Version]
  16. Giacomino, A.; Malandrino, M.; Colombo, M.L.; Miaglia, S.; Maimone, P.; Blancato, S.; Conca, E.; Abollino, O. Metal content in dandelion (Taraxacum officinale) leaves: Influence of vehicular traffic and safety upon consumption as food. J. Chem. 2016, 2016, 9842987. [Google Scholar] [CrossRef] [Green Version]
  17. Jastrzebska-Stojko, Z.; Stojko, R.; Rzepecka-Stojko, A.; Kabała-Dzik, A.; Stojko, J. Biological activity of propolis-honey balm in the treatment of experimentally-evoked burn wounds. Molecules 2013, 18, 14397–14413. [Google Scholar] [CrossRef] [Green Version]
  18. Miraj, S.; Rafieian, K.; Kiani, S. Melissa officinalis L.: A review study with an antioxidant prospective. J. Evid. Based Complement. Alter Med. 2017, 22, 385–394. [Google Scholar] [CrossRef]
  19. Gonzalez-Castejon, M.; Visioli, F.; Rodriguez-Casado, A. Diverse biological activities of dandelion. Nutr. Rev. 2012, 70, 534–547. [Google Scholar] [CrossRef]
  20. Mahboubi, M.; Mahboubi, M. Hepatoprotection by dandelion (Taraxacum officinale) and mechanisms. Asian Pac. J. Trop. Biomed. 2020, 10, 1–10. [Google Scholar] [CrossRef]
  21. Zahedifar, M.; Moosavi, A.A.; Zarei, Z.; Shafigh, M.; Karimian, F. Heavy metals content and distribution in basil (Ocimum basilicum L.) as influenced by cadmium and different potassium sources. Int. J. Phytorem. 2019, 21, 435–447. [Google Scholar] [CrossRef] [PubMed]
  22. Fattahia, B.; Arzania, K.; Souria, M.K.; Barzegar, M. Effects of cadmium and lead on seed germination, morphological traits, and essential oil composition of sweet basil (Ocimum basilicum L.). Ind. Crop. Prod. 2019, 138, 111584. [Google Scholar] [CrossRef]
  23. PN-ISO 10390:1997; Agricultural Chemical Analysis of the Soil. Determination of pH; ISO: Geneva, Switzerland, 1997. Available online: http://sklep.pkn.pl/pn-iso-10390-1997p.html (accessed on 20 April 2019).
  24. ASTM D2974-00, 2000; Standard Test Methods for Moisture, Ash, and Organic Matter of Peat and Other Organic Soils. American Society for Testing and Materials: West Conshohocken, PA, USA, 2000.
  25. Schumacher, B.A. Methods for the Determination of Total Organic Carbon (TOC) in Soils and Sediments; United States Environmental Protection Agency Environmental Sciences Division National Exposure Research Laboratory: Las Vegas, NV, USA, 2002. [Google Scholar]
  26. World Soil Resources Reports No. 103; IUSS Working Group WRB World Reference Base for Soil Resources. FAO: Rome, Italy, 2006.
  27. Regulation of the Minister of Environment. Item 1395, 1 August 2016.
  28. Bordens, K.S.; Abbott, B.B. Research Design and Methods. A Process Approach, 8th ed.; McGraw-Hill: New York, NY, USA, 2011; pp. 432–450. [Google Scholar]
  29. Galal, T.M.; Shehata, H.S. Bioaccumulation and translocation of heavy metals by Plantago major L. grown in contaminated soils under the effect of traffic pollution. Ecol. Ind. 2015, 48, 244–251. [Google Scholar] [CrossRef]
  30. Liu, K.; Lv, J.; He, W.; Zhang, H.; Cao, Y.; Dai, Y. Major factors influencing cadmium uptake from the soil into wheat plants. Ecotoxicol. Environ. Saf. 2015, 113, 207–213. [Google Scholar] [CrossRef] [PubMed]
  31. Testiati, E.; Parinet, J.; Massiani, C.; Laffont-Schwob, I.; Rabier, J.; Pfeifer, H.-R.; Lenoble, V.; Masotti, V.; Prudent, P. Trace metal and metalloid contamination levels in soils and two native plant species of a former industrial site: Evaluation of the phytostabilization potential. J. Hazard. Mater. 2013, 248, 131–141. [Google Scholar] [CrossRef]
  32. Adamczyk-Szabela, D.; Lisowska, K.; Wolf, W.M. Hysteresis of heavy metals uptake induced in Taraxacum offcinale by thiuram. Sci. Rep. 2021, 11, 20151. [Google Scholar] [CrossRef] [PubMed]
  33. Bradl, H.B. Adsorption of heavy metal ions on soils and soils constituents. J. Coll. Int. Sci. 2004, 277, 1–18. [Google Scholar] [CrossRef] [PubMed]
  34. Kabata-Pendias, A.; Pendias, H. Biogeochemistry of Trace Elements, 2nd ed.; PWN: Warsaw, Poland, 1999. [Google Scholar]
  35. Zhao, D.; Hao, Z.; Wang, J.; Tao, J. Effects of pH in irrigation water on plant growth and flower quality in Herbaceous peony (Paeonia lactiflora Pall.). Sci. Hortic. 2013, 154, 45–53. [Google Scholar] [CrossRef]
  36. Galindo-Castañeda, T.; Lynch, J.P.; Six, J.; Hartmann, M. Improving soil resource uptake by plants through capitalizing on synergies between root architecture and anatomy and root-associated microorganisms. Front. Plant Sci. 2022, 13, 827369. [Google Scholar] [CrossRef]
  37. Adamczyk-Szabela, D.; Markiewicz, J.; Wolf, W.M. Heavy Metal Uptake by Herbs. IV. Influence of soil pH on the content of heavy metals in Valeriana officinalis L. Water Air Soil Pollut. 2015, 226, 106. [Google Scholar] [CrossRef] [Green Version]
  38. Yan, A.; Wang, Y.; Tan, S.N.; Mohd Yusof, M.L.; Ghosh, S.; Chen, Z. Phytoremediation: A promising approach for revegetation of heavy metal-polluted land. Front. Plant Sci. 2020, 11, 359. [Google Scholar] [CrossRef] [PubMed]
  39. Jiang, Y.; Zeng, Q.; Wei, J.; Jiang, J.; Li, Y.; Chen, J.; Yu, H. Growth, fruit yield, photosynthetic characteristics, and leaf microelement concentration of two blueberry cultivars under different long-term soil pH treatments. Agronomy 2019, 9, 357. [Google Scholar] [CrossRef] [Green Version]
  40. Wu, D.; Liu, Y.; Liu, Y.; Dong, Y.; Chen, J.; Feng, Y.; Du, J. Study on available copper content in cultivated soils and its affecting factors in Zhuhai. In Proceedings of the International Conference on Computer Distributed Control and Intelligent Environmental Monitoring, Zhangjiajie, China, 5–6 March 2011; pp. 1350–1353. [Google Scholar]
  41. Rosas, A.; Rengel, Z.; de la Luz Mora, M. Manganese supply and pH influence growth, carboxylate exudation and peroxidase activity of ryegrass and white clover. J. Plant Nutr. 2007, 30, 253–270. [Google Scholar] [CrossRef]
  42. Pinto, E.; Aguiar, A.A.R.M.; Ferreira, I.M.P.L.V.O. Influence of soil chemistry and plant physiology in the phytoremediation of Cu, Mn, and Zn. Crit. Rev. Plant Sci. 2014, 33, 351–373. [Google Scholar] [CrossRef] [Green Version]
  43. Leitenmaier, B.; Kupper, H. Cadmium uptake and sequestration kinetics in individual leaf cell protoplasts of the Cd/Zn hyperaccumulator Thlaspi caerulescens. Plant Cell Environ. 2011, 34, 208–221. [Google Scholar] [CrossRef] [Green Version]
  44. Comin, J.J.; Ambrosini, V.G.; Rosa, D.J.; Basso, A.; Loss, A.; Bastos de Melo, G.W.; Lovato, P.E.; Lourenzi, C.R.; Ricachenevsky, F.K.; Brunetto, G. Liming as a means of reducing copper toxicity in black oats. Ciência Rural 2018, 48, 4. [Google Scholar] [CrossRef]
  45. Yruela, I. Copper in plants: Acquisition, transport and interactions. Funct. Plant Biol. 2009, 36, 409–430. [Google Scholar] [CrossRef] [Green Version]
  46. Humphries, J.; Stangoulis, J.; Graham, R. Manganese. In Handbook of Plant Nutrition; Barker, A., Pilbeam, D., Eds.; Taylor and Francis: Oxfordshire, UK, 2007; pp. 351–366. [Google Scholar]
  47. Millaleo, R.; Reyes-Diaz, M.; Ivanov, A.G.; Mora, M.L.; Alberdi, M. Manganese as essential and toxic element for plants: Transport, accumulation and resistance mechanisms. J. Soil Sci. Plant Nutr. 2010, 10, 476–494. [Google Scholar] [CrossRef] [Green Version]
  48. Olsen, L.I.; Palmgren, M.G. Many rivers to cross: The journey of zinc from soil to seed. Front. Plant Sci. 2014, 5, 1–6. [Google Scholar] [CrossRef] [Green Version]
  49. PN-ISO 10381-4: 2007; Soil quality—Sampling—Part 4: Rules for Procedure during the Research Areas of Natural, Semi-Natural and Cultivated. ISO: Geneva, Switzerland, 2007.
  50. PN-92R-04016; Agrochemical Analysis of Soil. Determination of Available Zinc Content. PL-PKN: Warasaw, Poland, 1993.
  51. Piotrowski, K.; Romanowska-Duda, Z.B.; Grzesik, M. How Biojodis and cyanobacteria alleviate the negative infuence of predicted environmental constraints on growth and physiological activity of corn plants. Pol. J. Environ. Stud. 2016, 25, 741–751. [Google Scholar] [CrossRef]
  52. Kalaji, H.M.; Schansker, G.; Brestic, M.; Bussotti, F.; Calatayud, A.; Ferroni, L.; Goltsev, V.; Guidi, L.; Jajoo, A.; Li, P.; et al. Frequently asked questions about chlorophyll fuorescence, the sequel. Photosynth. Res. 2017, 132, 13–66. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  53. Dybczyński, R.; Danko, B.; Kulisa, K.; Maleszewska, E.; Motrenko, H.P.; Samczyński, Z.; Szopa, Z. Preparation and preliminary certifcation of two new Polish CRMs for inorganic trace analysis. J. Radioanal. Nucl. Chem. 2004, 259, 409–413. [Google Scholar] [CrossRef]
Molecules 27 04671 g001 550
Figure 1. The effect of soil pH on the height of plants, index of chlorophyll, net photosynthesis, stomatal conductance, transpiration rate and intercellular concentration of CO2. Specific letters illustrate the statistically significant differences as computed with the Tukey’s HSD test (p = 0.95).
Figure 1. The effect of soil pH on the height of plants, index of chlorophyll, net photosynthesis, stomatal conductance, transpiration rate and intercellular concentration of CO2. Specific letters illustrate the statistically significant differences as computed with the Tukey’s HSD test (p = 0.95).
Molecules 27 04671 g001
Molecules 27 04671 g002 550
Figure 2. Manganese, copper and contents in above-ground parts and roots of basil, dandelion and lemon balm plants displayed against the pH modifications. Specific letters illustrate the statistically significant differences as computed with the Tukey’s HSD test (p = 0.95); roots and above-ground parts are treated independently.
Figure 2. Manganese, copper and contents in above-ground parts and roots of basil, dandelion and lemon balm plants displayed against the pH modifications. Specific letters illustrate the statistically significant differences as computed with the Tukey’s HSD test (p = 0.95); roots and above-ground parts are treated independently.
Molecules 27 04671 g002
Table
Table 1. Analysis of soil without additives (a). Bioavailable forms of Mn, Cu, Zn in either initial soil without additives (pH = 6.0) or modified soil samples with pH adjusted to 4.7 and 8.7, respectively (b). Specific letters illustrate the statistically significant differences as computed with the Tukey’s HSD test (p = 0.95).
Table 1. Analysis of soil without additives (a). Bioavailable forms of Mn, Cu, Zn in either initial soil without additives (pH = 6.0) or modified soil samples with pH adjusted to 4.7 and 8.7, respectively (b). Specific letters illustrate the statistically significant differences as computed with the Tukey’s HSD test (p = 0.95).
(a)
pH6.0
Organic matter32.5%
Metal concentration (µg·g−1)MnCuZn
Total forms198 ± 525.6 ± 0.6201 ± 4
(b)
Bioavailable formspH = 4.7133 ± 1.7 A14.6 ± 0.7 D136 ± 6 F
pH = 6.0
control		98.7 ± 1.8 B12.2 ± 0.7 E117 ± 2 G
pH = 8.7119 ± 2 C15.4 ± 0.5 D91.8 ± 0.9 H
Table
Table 2. The one-way ANOVA for manganese, copper, zinc contents in plant across the soil pH. Critical Snedecor’s F value is Fcryt = 3.88533.
Table 2. The one-way ANOVA for manganese, copper, zinc contents in plant across the soil pH. Critical Snedecor’s F value is Fcryt = 3.88533.
BasilDandelionLemon Balm
Above-Ground PartsRootsAbove-Ground PartsRootsAbove-Ground PartsRoots
Mnp = 8.17 × 10−10
F = 190.2278		p = 1.37 × 10−9
F = 174.0622		p = 7.47 × 10−7
F = 56.9940		p = 6.10 × 10−9
F = 134.3608		p = 2.59 × 10−9
F = 155.8941		p = 8.43 × 10−8
F = 84.6094
Cup = 1.02 × 10−7
F = 81.8463		p = 5.61 × 10−11
F = 300.6741		p = 1.56 × 10−7
F = 75.7392		p = 5.96 × 10−7
F = 59.3978		p = 6.60 × 10−6
F = 37.8033		p = 1.60 × 10−8
F = 113.4664
Znp = 9.68 × 10−6
F = 35.0993		p = 1.80 × 10−5
F = 31.0742		p = 1.07 × 10−6
F = 53.3690		p = 1.14 × 10−5
F = 33.9830		p = 7.33 × 10−8
F = 86.7552		p = 1.06 × 10−5
F = 34.4554
Table
Table 3. Bioaccumulation factors (BAF), translocation factors (TF) and transfer coefficients (TC) calculated for basil, lemon balm and dandelion, respectively. Elements are shown in decreasing order of particular factor. Each value is the average of the data from five replicates.
Table 3. Bioaccumulation factors (BAF), translocation factors (TF) and transfer coefficients (TC) calculated for basil, lemon balm and dandelion, respectively. Elements are shown in decreasing order of particular factor. Each value is the average of the data from five replicates.
BAFTFTC
pHBasil
4.7Mn (1.05) > Zn (0.61) > Cu (0.60)Mn (0.76) > Zn (0.55) > Cu (0.45)Mn (1.39) > Cu (1.32) > Zn (1.11)
6.0 controlMn (0.58) > Zn (0.50) > Cu (0.36)Mn (0.68) > Zn (0.50) = Cu (0.50)Zn (0.99) > Mn (0.85) > Cu (0.73)
8.5Mn (0.52) > Zn (0.41) > Cu (0.30)Mn (0.72) > Zn (0.48) > Cu (0.47)Zn (0.86) > Mn (0.72) > Cu (0.60)
Dandelion
4.7Mn (0.87) > Zn (0.61) > Cu (0.39)Cu (2.24) > Mn (1.67) > Zn (0.65)Zn (1.02) > Mn (0.52) > Cu (0.40)
6.0 controlMn (0.75) > Zn (0.51) > Cu (0.47)Cu (1.58) > Mn (1.53) > Zn (0.52)Zn (0.97) > Mn (0.49) > Cu (0.48)
8.5Mn (1.10) > Cu (0.26) > Zn (0.25)Cu (1.83) > Mn (1.32) > Zn (0.55)Mn (0.83) > Zn (0.78) > Cu (0.22)
Lemon balm
4.7Mn (1.17) > Zn (0.92) > Cu (0.33)Mn (1.49) > Zn (0.84) > Cu (0.54)Zn (1.10) > Mn (0.79) > Cu (0.61)
6.0 controlZn (0.74) > Cu (0.64) > Mn (0.56)Mn (1.19) > Zn (0.74) > Cu (0.65)Zn (1.00) > Cu (0.98) > Mn (0.47)
8.5Mn (1.06) > Zn (0.48) > Cu (0.47)Mn (1.60) > Zn (0.57) > Cu (0.62)Zn (0.84) > Cu (0.76) > Mn (0.55)
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Adamczyk-Szabela, D.; Wolf, W.M. The Impact of Soil pH on Heavy Metals Uptake and Photosynthesis Efficiency in Melissa officinalis, Taraxacum officinalis, Ocimum basilicum. Molecules 2022, 27, 4671. https://doi.org/10.3390/molecules27154671

AMA Style

Adamczyk-Szabela D, Wolf WM. The Impact of Soil pH on Heavy Metals Uptake and Photosynthesis Efficiency in Melissa officinalis, Taraxacum officinalis, Ocimum basilicum. Molecules. 2022; 27(15):4671. https://doi.org/10.3390/molecules27154671

Chicago/Turabian Style

Adamczyk-Szabela, Dorota, and Wojciech M. Wolf. 2022. "The Impact of Soil pH on Heavy Metals Uptake and Photosynthesis Efficiency in Melissa officinalis, Taraxacum officinalis, Ocimum basilicum" Molecules 27, no. 15: 4671. https://doi.org/10.3390/molecules27154671

Article Metrics

Back to TopTop