Evaluation of an Element-Tagged Duplex Immunoassay Coupled with Inductively Coupled Plasma Mass Spectrometry Detection: A Further Study for the Application of the New Assay in Clinical Laboratory
Abstract
:1. Introduction
2. Results
2.1. Evaluation of the ICP-MS Instrument
2.2. Optimization of the Assay
2.3. Evaluation of Calibration Curves
2.4. Evaluation of the Imprecision
2.5. Evaluation of the Accuracy
2.6. Establishment of Reference Interval
2.7. Detection of Clinical Samples
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Instruments and Reagents
5.2. Clinical Samples
5.3. Evaluation of the ICP-MS Analyzer
5.4. Assay Protocol and Its Optimization
5.5. Evaluation of the Calibration Curves
5.6. Evaluation of the Imprecision
5.7. Evaluation of the Accuracy
5.8. Establishment of Reference Interval
5.9. Clinical Sample Detection
5.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
- Jaffe, A.S.; Januzzi, J.L., Jr. Using Biomarkers to Guide Heart Failure Therapy. Clin. Chem. 2017, 63, 954–957. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Mukherjee, S.; Baidoo, J.N.E.; Sampat, S.; Mancuso, A.; David, L.; Cohen, L.S.; Zhou, S.; Banerjee, P. Liposomal TriCurin, A Synergistic Combination of Curcumin, Epicatechin Gallate and Resveratrol, Repolarizes Tumor-Associated Microglia/Macrophages, and Eliminates Glioblastoma (GBM) and GBM Stem Cells. Molecules 2018, 23, 201. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Okarvi, S.M.; AlJammaz, I. Development of the Tumor-Specific Antigen-Derived Synthetic Peptides as Potential Candidates for Targeting Breast and Other Possible Human Carcinomas. Molecules 2019, 24, 3142. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- de Ory, F.; Minguito, T.; Balfagon, P.; Sanz, J.C. Comparison of chemiluminescent immunoassay and ELISA for measles IgG and IgM. APMIS 2015, 123, 648–651. [Google Scholar] [CrossRef] [PubMed]
- Yalow, R.S.; Berson, S.A. Assay of plasma insulin in human subjects by immunological methods. Nature 1959, 184, 1648–1649. [Google Scholar] [CrossRef] [PubMed]
- Liu, R.; Wu, P.; Yang, L.; Hou, X.; Lv, Y. Inductively coupled plasma mass spectrometry-based immunoassay: A review. Mass Spectrom. Rev. 2014, 33, 373–393. [Google Scholar] [CrossRef] [PubMed]
- Aydin, S. A short history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using ELISA. Peptides 2015, 72, 4–15. [Google Scholar] [CrossRef]
- Hou, J.Y.; Liu, T.C.; Lin, G.F.; Li, Z.X.; Zou, L.P.; Li, M.; Wu, Y.S. Development of an immunomagnetic bead-based time-resolved fluorescence immunoassay for rapid determination of levels of carcinoembryonic antigen in human serum. Anal. Chim. Acta 2012, 734, 93–98. [Google Scholar] [CrossRef]
- Hua, X.; You, H.; Luo, P.; Tao, Z.; Chen, H.; Liu, F.; Wang, M. Upconversion fluorescence immunoassay for imidaclothiz by magnetic nanoparticle separation. Anal. Bioanal. Chem. 2017, 409, 6885–6892. [Google Scholar] [CrossRef]
- Liu, Q.-L.; Yan, X.-H.; Yin, X.-M.; Situ, B.; Zhou, H.-K.; Lin, L.; Li, B.; Gan, N.; Zheng, L. Electrochemical Enzyme-Linked Immunosorbent Assay (ELISA) for α-Fetoprotein Based on Glucose Detection with Multienzyme-Nanoparticle Amplification. Molecules 2013, 18, 12675–12686. [Google Scholar] [CrossRef]
- Liu, Z.; Huang, J.; Ou, R.M.; Yao, M.D.; She, Y.L.; Chen, R.; Li, C.; Xu, L.; Abudureyimu, A.; Zhang, Q.; et al. A dual-label time-resolved fluorescence immunoassay for the simultaneous determination of ferritin and beta2 -microglobulin. J. Clin. Lab. Anal. 2017, 31, e22132. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Wang, C.; Jiang, T.; Zhao, K.; Deng, A.; Li, J. A novel electrochemiluminescent immunoassay for diclofenac using conductive polymer functionalized graphene oxide as labels and gold nanorods as signal enhancers. Talanta 2019, 193, 184–191. [Google Scholar] [CrossRef] [PubMed]
- Xue, T.Y.; Mei, L.P.; Xu, Y.T.; Liu, Y.L.; Fan, G.C.; Li, H.Y.; Ye, D. Nanoporous Semiconductor Electrode Captures the Quantum Dots: Toward Ultrasensitive Signal-On Liposomal Photoelectrochemical Immunoassay. Anal. Chem. 2019, 91, 3795–3799. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Hu, Q.; Wei, Q.; Zhang, P.; Li, S.; Xue, L.; Yang, R.; Wang, C.; Zhou, L. An up-converting phosphor technology-based lateral flow assay for point-of-collection detection of morphine and methamphetamine in saliva. Analyst 2018, 143, 4646–4654. [Google Scholar] [CrossRef] [PubMed]
- Angelo, M.; Bendall, S.C.; Finck, R.; Hale, M.B.; Hitzman, C.; Borowsky, A.D.; Levenson, R.M.; Lowe, J.B.; Liu, S.D.; Zhao, S.; et al. Multiplexed ion beam imaging of human breast tumors. Nat. Med. 2014, 20, 436–442. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Biju, V.; Itoh, T.; Ishikawa, M. Delivering quantum dots to cells: Bioconjugated quantum dots for targeted and nonspecific extracellular and intracellular imaging. Chem. Soc. Rev. 2010, 39, 3031–3056. [Google Scholar] [CrossRef]
- Valekova, I.; Skalnikova, H.K.; Jarkovska, K.; Motlik, J.; Kovarova, H. Multiplex immunoassays for quantification of cytokines, growth factors, and other proteins in stem cell communication. Methods Mol. Biol. 2015, 1212, 39–63. [Google Scholar] [CrossRef]
- Wang, X.; Wang, X.; Qin, W.; Lin, H.; Wang, J.; Wei, J.; Zhang, Y.; Qian, X. Metal-tag labeling coupled with multiple reaction monitoring-mass spectrometry for absolute quantitation of proteins. Analyst 2013, 138, 5309–5317. [Google Scholar] [CrossRef] [Green Version]
- Zhang, C.; Wu, F.; Zhang, Y.; Wang, X.; Zhang, X. A novel combination of immunoreaction and ICP-MS as a hyphenated technique for the determination of thyroid-stimulating hormone (TSH) in human serum. J. Anal. At. Spectrom. 2001, 16, 1393–1396. [Google Scholar] [CrossRef]
- Zhang, S.; Zhang, C.; Xing, Z.; Zhang, X. Simultaneous determination of alpha-fetoprotein and free beta-human chorionic gonadotropin by element-tagged immunoassay with detection by inductively coupled plasma mass spectrometry. Clin. Chem. 2004, 50, 1214–1221. [Google Scholar] [CrossRef] [Green Version]
- Zhang, Y.; Sun, G.; Zhang, Y.; Huang, B.; Xing, Z.; Zhang, S.; Zhang, X. Simultaneous competitive and sandwich formats multiplexed immunoassays based on ICP-MS detection. Talanta 2018, 185, 237–242. [Google Scholar] [CrossRef] [PubMed]
- Ornatsky, O.; Baranov, V.I.; Bandura, D.R.; Tanner, S.D.; Dick, J. Multiple cellular antigen detection by ICP-MS. J. Immunol. Methods 2006, 308, 68–76. [Google Scholar] [CrossRef] [PubMed]
- Sun, G.; Huang, B.; Zhang, Y.; Zhang, Y.; Xing, Z.; Zhang, S.; Zhang, X. A combinatorial immunoassay for multiple biomarkers via a stable isotope tagging strategy. Chem. Commun. 2017, 53, 13075–13078. [Google Scholar] [CrossRef]
- Bjornson, Z.B.; Nolan, G.P.; Fantl, W.J. Single-cell mass cytometry for analysis of immune system functional states. Curr. Opin. Immunol. 2013, 25, 484–494. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Bendall, S.C.; Simonds, E.F.; Qiu, P.; Amir el, A.D.; Krutzik, P.O.; Finck, R.; Bruggner, R.V.; Melamed, R.; Trejo, A.; Ornatsky, O.I.; et al. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science 2011, 332, 687–696. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Mueller, L.; Herrmann, A.J.; Techritz, S.; Panne, U.; Jakubowski, N. Quantitative characterization of single cells by use of immunocytochemistry combined with multiplex LA-ICP-MS. Anal. Bioanal. Chem. 2017, 409, 3667–3676. [Google Scholar] [CrossRef] [PubMed]
- Yang, B.; Zhang, Y.; Chen, B.; He, M.; Hu, B. Elemental-tagged immunoassay combined with inductively coupled plasma mass spectrometry for the detection of tumor cells using a lead sulfide nanoparticle label. Talanta 2017, 167, 499–505. [Google Scholar] [CrossRef]
- Hu, Z.; Sun, G.; Jiang, W.; Xu, F. Chemical-Modified Nucleotide-Based Elemental Tags for High-Sensitive Immunoassay. Anal. Chem. 2019, 91, 5980–5986. [Google Scholar] [CrossRef]
- Zhang, X.; Careri, M.; Elviri, L.; Maffini, M.; Mangia, A.; Mucchino, C.; Terenghi, M. Determination of peanut allergens in cereal-chocolate-based snacks: Metal-tag inductively coupled plasma mass spectrometry immunoassay versus liquid chromatography/electrospray ionization tandem mass spectrometry. Anal. Chem. 2008, 22, 807–811. [Google Scholar]
- Liu, Z.; Chen, B.; He, M.; Zhang, X.; Wang, H.; Hu, B. Application of inductively coupled plasma mass spectrometry in the study of apoptosis: Determination of caspase-3 using a gold nanoparticle tag. Analyst 2016, 141, 926–933. [Google Scholar] [CrossRef]
- Jiang, W.; Sun, G.; Wen, X.; Men, S.; Cui, W.; Jing, M.; Jia, X.; Hu, Z.; Pu, D.; Zhang, S.; et al. Development and evaluation of an element-tagged immunoassay coupled with inductively coupled plasma mass spectrometry detection: Can we apply the new assay in the clinical laboratory? Clin. Chem. Lab. Med. 2020, 58, 873–882. [Google Scholar] [CrossRef] [PubMed]
- Qi, F.; Zhou, A.; Yan, L.; Yuan, X.; Wang, D.; Chang, R.; Zhang, Y.; Shi, F.; Han, X.; Hou, J.; et al. The diagnostic value of PIVKA-II, AFP, AFP-L3, CEA, and their combinations in primary and metastatic hepatocellular carcinoma. J. Clin. Lab. Anal. 2019, 34, e23158. [Google Scholar] [CrossRef]
- Ito, H.; Hoshi, K.; Honda, T.; Hashimoto, Y. Lectin-Based Assay for Glycoform-Specific Detection of α2,6-sialylated Transferrin and Carcinoembryonic Antigen in Tissue and Body Fluid. Molecules 2018, 23, 1314. [Google Scholar] [CrossRef] [Green Version]
- Perez, E.; Bierla, K.; Grindlay, G.; Szpunar, J.; Mora, J.; Lobinski, R. Lanthanide polymer labels for multiplexed determination of biomarkers in human serum samples by means of size exclusion chromatography-inductively coupled plasma mass spectrometry. Anal. Chim. Acta 2018, 1018, 7–15. [Google Scholar] [CrossRef] [Green Version]
- Hutchinson, R.W.; Cox, A.G.; McLeod, C.W.; Marshall, P.S.; Harper, A.; Dawson, E.L.; Howlett, D.R. Imaging and spatial distribution of beta-amyloid peptide and metal ions in Alzheimer’s plaques by laser ablation-inductively coupled plasma-mass spectrometry. Anal. Biochem. 2005, 346, 225–233. [Google Scholar] [CrossRef]
- Bjerner, J.; Høgetveit, A.; Wold Akselberg, K.; Vangsnes, K.; Paus, E.; Bjøro, T.; Børmer, O.P.; Nustad, K. Reference intervals for carcinoembryonic antigen (CEA), CA125, MUC1, Alfa-foeto-protein (AFP), neuron-specific enolase (NSE) and CA19.9 from the NORIP study. Scand. J. Clin. Lab. Investig. 2008, 68, 703–713. [Google Scholar] [CrossRef]
- CLSI. Validation, Verification, and Quality Assurance of Automated Hematology Analyzers. In CLSI Document H26-A2, 2nd ed.; Clinical and Laboratory Standards Institute: Wayne, PA, USA, 2010. [Google Scholar]
- CLSI. Evaluation of Detection Capability for Clinical Laboratory Measurement Procedures. In CLSI Document EP17-A2, 2nd ed.; Clinical and Laboratory Standards Institute: Wayne, PA, USA, 2012. [Google Scholar]
- CLSI. Establishing and Verifying an Extended Measuring Interval Through Specimen Dilution and Spiking. In CLSI Document EP34, 1st ed.; Clinical and Laboratory Standards Institute: Wayne, PA, USA, 2018. [Google Scholar]
- NCCLS. Evaluation of the Linearity of Quantitative Measurement Procedures; A Statistical Approach; Approved Guideline. In NCCLS Document EP6-A; Clinical and Laboratory Standards Institute: Wayne, PA, USA, 2003. [Google Scholar]
- CLSI. Evaluation of Precision of Quantitative Measurement Procedures. In CLSI Document EP05-A3, 3rd ed.; Clinical and Laboratory Standards Institute: Wayne, PA, USA, 2014. [Google Scholar]
- CLSI. Measurement Procedure Comparision and Bias Estimation Using Patiengt Samples. In CLSI Document EP09-A3, 3rd ed.; Clinical and Laboratory Standards Institute: Wayne, PA, USA, 2013. [Google Scholar]
- CLSI. Interference Testing in Clinical Chemistry. In CLSI Document EP07-A2, 2nd ed.; Clinical and Laboratory Standards Institute: Wayne, PA, USA, 2014. [Google Scholar]
- CLSI. Defining, Establishing, and Verifying Reference Intervals in the Clinical Laboratory. In CLSI Document C28-A3, 3rd ed.; Clinical and Laboratory Standards Institute: Wayne, PA, USA, 2008. [Google Scholar]
Parameter | Value |
---|---|
cool gas flow (L/min) | 13 |
auxiliary gas flow (L/min) | 0.7 |
nebulizer gas flow (L/min) | 0.96 |
sample uptake (s) | 38 |
dwell time (ms) | 0.05 |
channel | 3 |
number of repeats per sample | 3 |
PC detector voltage (V) | 1265 |
RF power (W) | 1578.61 |
analogue detector voltage (V) | −1960 |
Sample availability: Samples of the compounds are not available from the authors. | |
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Share and Cite
Jiang, W.; Sun, G.; Cui, W.; Men, S.; Jing, M.; Pu, D.; Zhang, S.; Yuan, X.; Zhang, X.; Wang, C. Evaluation of an Element-Tagged Duplex Immunoassay Coupled with Inductively Coupled Plasma Mass Spectrometry Detection: A Further Study for the Application of the New Assay in Clinical Laboratory. Molecules 2020, 25, 5370. https://doi.org/10.3390/molecules25225370
Jiang W, Sun G, Cui W, Men S, Jing M, Pu D, Zhang S, Yuan X, Zhang X, Wang C. Evaluation of an Element-Tagged Duplex Immunoassay Coupled with Inductively Coupled Plasma Mass Spectrometry Detection: A Further Study for the Application of the New Assay in Clinical Laboratory. Molecules. 2020; 25(22):5370. https://doi.org/10.3390/molecules25225370
Chicago/Turabian StyleJiang, Wencan, Gongwei Sun, Wenbin Cui, Shasha Men, Miao Jing, Danna Pu, Sichun Zhang, Xiaozhou Yuan, Xinrong Zhang, and Chengbin Wang. 2020. "Evaluation of an Element-Tagged Duplex Immunoassay Coupled with Inductively Coupled Plasma Mass Spectrometry Detection: A Further Study for the Application of the New Assay in Clinical Laboratory" Molecules 25, no. 22: 5370. https://doi.org/10.3390/molecules25225370