Effects of Ultrasonic-Assisted Extraction on the Physicochemical Properties of Different Walnut Proteins
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Ultrasonic-Assisted Extraction of Walnut Proteins
2.3. Determination of Ultrasonic Time and Power Output
2.4. Effect of Ultrasonic-Assisted Extraction on Structural Properties
2.4.1. SDS-PAGE
2.4.2. Secondary Structure
2.4.3. Surface Hydrophobicity
2.4.4. Contents of Total and Surface Free Sulfhydryl
2.4.5. Intrinsic Fluorescence
2.4.6. Particle Size Distribution
2.4.7. Scanning Electron Microscopy
2.5. Effect of Ultrasonic-Assisted Extraction on Functional Properties
2.5.1. Protein Solubility
2.5.2. Emulsifying Activity (EA) and Emulsion Stability (ES)
2.5.3. Foaming Capacity (FC) and Foam Stability (FS)
2.6. Statistical Analysis
3. Results and Discussion
3.1. Determination of Conditions for Ultrasonic-Assisted Extraction
3.2. Effect of Ultrasonic-Assisted Extraction on Structural Properties
3.2.1. SDS-PAGE
3.2.2. Secondary Structure
3.2.3. Surface Hydrophobicity
3.2.4. Total and Surface Free Sulfhydryl
3.2.5. Intrinsic Fluorescence
3.2.6. Particle Size Distribution
3.2.7. Scanning Electron Microscopy
3.3. Effect of Ultrasound-Assisted Extraction on the Functional Properties
3.3.1. Solubility Property
3.3.2. Emulsifying Property
3.3.3. Foaming Property
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: not available. |
Ultrasonic Extraction | Conditions | Energy Density (J/mL) |
---|---|---|
Diameter titanium probe (cm) | 0.636 | / |
Frequency (kHz) | 20 | / |
Power output (W) | 0, 100, 200, 300, 400, 500 | 0, 44, 88, 132, 176, 220 |
Time (min) | 0, 5, 10, 15, 20, 25 | 0, 22, 44, 66, 88, 110 |
pulse duration (s) | on-time 4/off-time 2 | / |
Samples | D43 (μm) | D32 (μm) |
---|---|---|
A (albumin of the control) | 26.24 ± 0.22 | 9.38 ± 0.02 |
B (albumin of UAE) | 18.57 ± 0.19 | 8.21 ± 0.01 |
C (globulin of the control) | 33.59 ± 0.58 | 6.88 ± 0.02 |
D (globulin of UAE) | 40.89 ± 0.23 | 9.70 ± 0.02 |
E (glutelin of the control) | 157.50 ± 1.06 | 63.33 ± 0.04 |
F (glutelin of UAE) | 138.28 ± 0.99 | 48.62 ± 0.33 |
Samples | α-Helix (%) | β-Sheet (%) | β-Turn (%) | Random Coil (%) |
---|---|---|---|---|
A (albumin of the control) | 15.73 ± 0.21 | 55.83 ± 0.11 | 0.1 ± 0.02 | 28.30 ± 0.11 |
B (albumin of UAE) | 26.57 ± 0.26 | 45.87 ± 0.50 | 0 ± 0.0 | 27.60 ± 0.24 |
C (globulin of the control) | 16.63 ± 0.14 | 11.63 ± 0.46 | 27.77 ± 0.26 | 43.97 ± 0.12 |
D (globulin of UAE) | 19.93 ± 0.32 | 22.10 ± 0.33 | 21.67 ± 0.52 | 36.30 ± 0.13 |
E (glutelin of the control) | 2.10 ± 0.07 | 43.67 ± 0.57 | 6.5 ± 0.28 | 47.73 ± 0.45 |
F (glutelin of UAE) | 0 ± 0 | 39.33 ± 0.09 | 11.87 ± 0.06 | 48.77 ± 0.07 |
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Lv, S.; Taha, A.; Hu, H.; Lu, Q.; Pan, S. Effects of Ultrasonic-Assisted Extraction on the Physicochemical Properties of Different Walnut Proteins. Molecules 2019, 24, 4260. https://doi.org/10.3390/molecules24234260
Lv S, Taha A, Hu H, Lu Q, Pan S. Effects of Ultrasonic-Assisted Extraction on the Physicochemical Properties of Different Walnut Proteins. Molecules. 2019; 24(23):4260. https://doi.org/10.3390/molecules24234260
Chicago/Turabian StyleLv, Siyi, Ahmed Taha, Hao Hu, Qi Lu, and Siyi Pan. 2019. "Effects of Ultrasonic-Assisted Extraction on the Physicochemical Properties of Different Walnut Proteins" Molecules 24, no. 23: 4260. https://doi.org/10.3390/molecules24234260