Functional Roles of Homologous Recombination and Non-Homologous End Joining in DNA Damage Response and Microevolution in Cryptococcus neoformans
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Growth Condition
2.2. Construction of Strains
2.3. Construction of KU70, KU80, and RAD51 Complemented Strains
2.4. Spotting Assay
2.5. Total RNA Isolation, cDNA Synthesis, and Real-Time Quantitative PCR (qRT-PCR)
2.6. Mutation Rate Assay
2.7. Fluconazole Resistance Assay
2.8. Phenotypic Characterization of Microevolution of C. neoformans Strains
2.9. Identification of DNA Mutations
3. Results
3.1. Expression of KU70 and KU80 Is Induced in Response to DNA Damage Stress in a Rad53–Bdr1 Pathway-Dependent Manner
3.2. Ku70/Ku80 and Rad51 Cooperatively Regulate DNA Damage Response
3.3. Ku70/Ku80 and Rad51 Are Involved in the Oxidative Stress Response
3.4. Functional Role of Accumulation of DNA Mutations and Microevolution Mediated by HR and NHEJ
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Jung, K.-W.; Jung, J.-H.; Park, H.-Y. Functional Roles of Homologous Recombination and Non-Homologous End Joining in DNA Damage Response and Microevolution in Cryptococcus neoformans. J. Fungi 2021, 7, 566. https://doi.org/10.3390/jof7070566
Jung K-W, Jung J-H, Park H-Y. Functional Roles of Homologous Recombination and Non-Homologous End Joining in DNA Damage Response and Microevolution in Cryptococcus neoformans. Journal of Fungi. 2021; 7(7):566. https://doi.org/10.3390/jof7070566
Chicago/Turabian StyleJung, Kwang-Woo, Jong-Hyun Jung, and Ha-Young Park. 2021. "Functional Roles of Homologous Recombination and Non-Homologous End Joining in DNA Damage Response and Microevolution in Cryptococcus neoformans" Journal of Fungi 7, no. 7: 566. https://doi.org/10.3390/jof7070566