Transcriptional Responses of Sclerotinia sclerotiorum to the Infection by SsHADV-1
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Material and Growth Conditions
2.2. Sample Collection and RNA Extraction
2.3. cDNA Library Preparation and Sequencing
2.4. RNA-Seq Data Analysis
2.5. The Detection of Oxalic Acid (OA)-Producing Ability of the Two Strains
2.6. Quantitative Real-Time RT-PCR (qRT-PCR) Analysis
3. Results
3.1. Overview of All RNA-Seq Data
3.2. Gene Ontology (GO) Enrichment Analysis for DEGs
3.3. KEGG Enrichment Analysis of DEGs
3.4. The Key Non-Homologous End Joining (NHEJ) Genes Were Up-Regulated in Strain DT-8
3.5. Most of the Antiviral RNA Silencing Genes Were Down-Regulated in Strain DT-8
3.6. SsHADV-1 Down-Regulated the Expression of Many Virulence Factor Genes
3.7. SsHADV-1 Did Not Influence the OA-Producing Ability
3.8. Gene Expression Level by qRT-PCR
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Up-Regulated Genes | Down-Regulated Genes | ||||
---|---|---|---|---|---|
InterPro ID | Description | No. of Genes | InterPro ID | Description | No. of Genes |
IPR036259 | MFS transporter superfamily | 73 | IPR036291 | NAD(P)-binding domain superfamily | 50 |
IPR020846 | Major facilitator superfamily domain | 71 | IPR027417 | P-loop containing nucleoside triphosphate hydrolase | 42 |
IPR027417 | P-loop containing nucleoside triphosphate hydrolase | 56 | IPR017853 | Glycoside hydrolase superfamily | 34 |
IPR036291 | NAD(P)-binding domain superfamily | 49 | IPR020846 | Major facilitator superfamily domain | 31 |
IPR011701 | Major facilitator superfamily | 41 | IPR029058 | Alpha/Beta hydrolase fold | 31 |
IPR029058 | Alpha/Beta hydrolase fold | 33 | IPR036259 | MFS transporter superfamily | 31 |
IPR036188 | FAD/NAD(P)-binding domain superfamily | 28 | IPR036397 | Ribonuclease H superfamily | 25 |
IPR029063 | S-adenosyl-L-methionine-dependent methyltransferase | 23 | IPR012337 | Ribonuclease H-like superfamily | 24 |
IPR016181 | Acyl-CoA N-acyltransferase | 22 | IPR029063 | S-adenosyl-L-methionine-dependent methyltransferase | 24 |
IPR005828 | Major facilitator, sugar transporter-like | 20 | IPR011701 | Major facilitator superfamily | 23 |
IPR002347 | Short-chain dehydrogenase/reductase SDR | 18 | IPR002347 | Short-chain dehydrogenase/reductase SDR | 22 |
IPR005829 | Sugar transporter, conserved site | 18 | IPR036188 | FAD/NAD(P)-binding domain superfamily | 21 |
IPR006600 | HTH CenpB-type DNA-binding domain | 18 | IPR036396 | Cytochrome P450 superfamily | 21 |
IPR036396 | Cytochrome P450 superfamily | 18 | IPR001128 | Cytochrome P450 | 20 |
IPR001128 | Cytochrome P450 | 17 | IPR001138 | Zn(2)-C6 fungal-type DNA-binding domain | 19 |
IPR003593 | AAA+ ATPase domain | 17 | IPR011009 | Protein kinase-like domain superfamily | 18 |
IPR000182 | GNAT domain | 16 | IPR009057 | Homeobox-like domain superfamily | 17 |
IPR002293 | Amino acid/polyamine transporter I | 16 | IPR036864 | Zn(2)-C6 fungal-type DNA-binding domain superfamily | 16 |
IPR003663 | Sugar/inositol transporter | 15 | IPR000477 | Reverse transcriptase domain | 15 |
IPR011009 | Protein kinase-like domain superfamily | 15 | IPR013087 | Zinc finger C2H2-type | 14 |
IPR017853 | Glycoside hydrolase superfamily | 15 | IPR020904 | Short-chain dehydrogenase/reductase, conserved site | 14 |
IPR036249 | Thioredoxin-like superfamily | 14 |
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Qu, Z.; Fu, Y.; Lin, Y.; Zhao, Z.; Zhang, X.; Cheng, J.; Xie, J.; Chen, T.; Li, B.; Jiang, D. Transcriptional Responses of Sclerotinia sclerotiorum to the Infection by SsHADV-1. J. Fungi 2021, 7, 493. https://doi.org/10.3390/jof7070493
Qu Z, Fu Y, Lin Y, Zhao Z, Zhang X, Cheng J, Xie J, Chen T, Li B, Jiang D. Transcriptional Responses of Sclerotinia sclerotiorum to the Infection by SsHADV-1. Journal of Fungi. 2021; 7(7):493. https://doi.org/10.3390/jof7070493
Chicago/Turabian StyleQu, Zheng, Yanping Fu, Yang Lin, Zhenzhen Zhao, Xuekun Zhang, Jiasen Cheng, Jiatao Xie, Tao Chen, Bo Li, and Daohong Jiang. 2021. "Transcriptional Responses of Sclerotinia sclerotiorum to the Infection by SsHADV-1" Journal of Fungi 7, no. 7: 493. https://doi.org/10.3390/jof7070493