Evolutionary Patterns of Sex-Biased Genes in Three Species of Haplodiploid Insects
Abstract
:1. Introduction
2. Materials and Methods
2.1. Whitefly Cultures and RNA Isolation
2.2. RNA-Sequencing, de novo Assembly for Reference Transcriptome Sequences, and Mapping for Samples
2.3. Identification of Orthologs and Analysis of Sequence Evolution
2.4. Normalization of Gene Expression Across Species
2.5. Analysis of the Global Pattern of Gene Expression Levels
2.6. Analysis of Gene Expression among Species and Sexes
3. Results
3.1. Summary of RNA Sequencing and Mapping
3.2. Analysis of Sex-Biased Genes
3.3. Evolution of Coding Sequences across Species
3.4. Global Pattern of Gene Expression across Sex and Species
3.5. Evolution of Gene Expression across Species
3.6. Sex-Biased Genes in Orthologs Groups
3.7. Evolution of Coding Sequences of Sex-Biased Genes
3.8. Expression Evolution of Sex-Biased Genes
3.9. Differential Expression across Sexes and Species
3.10. Correlations of Sex-Biased Expression and Evolution of Sequences and Expression
4. Discussion
4.1. Variation in Gene Expression Is More Influenced by Sex than by Species
4.2. Sex-Biased Genes Are Consistent among the Three Bemisia Species
4.3. Sex-Biased Genes in Whiteflies Show a Pattern of Gene Sequences and Expressions Different from That in Social Haplodiploid and Diploid Organisms
4.4. Possible Causes for the Slow Evolution of Sex-Biased Genes
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Wang, Y.-J.; Wang, H.-L.; Wang, X.-W.; Liu, S.-S. Evolutionary Patterns of Sex-Biased Genes in Three Species of Haplodiploid Insects. Insects 2020, 11, 326. https://doi.org/10.3390/insects11060326
Wang Y-J, Wang H-L, Wang X-W, Liu S-S. Evolutionary Patterns of Sex-Biased Genes in Three Species of Haplodiploid Insects. Insects. 2020; 11(6):326. https://doi.org/10.3390/insects11060326
Chicago/Turabian StyleWang, Yu-Jun, Hua-Ling Wang, Xiao-Wei Wang, and Shu-Sheng Liu. 2020. "Evolutionary Patterns of Sex-Biased Genes in Three Species of Haplodiploid Insects" Insects 11, no. 6: 326. https://doi.org/10.3390/insects11060326