Anti-Biofilm Activity of Assamsaponin A, Theasaponin E1, and Theasaponin E2 against Candida albicans
Abstract
:1. Introduction
2. Results
2.1. The Biofilm Growth Curve of Biofilm Formation of C. albicans ATCC 10231
2.2. ASA, TE1, and TE2 Inhibit the Adhesion of C. albicans
2.3. ASA, TE1, and TE2 Inhibit the Biofilm Formation and Mature Biofilm of C. albicans
2.4. ASA, TE1, and TE2 Reduce Cell Surface Hydrophobicity and Extracellular Phospholipase of C. albicans
2.5. Effects of ASA, TE1, and TE2 on Genes Associated with Virulence Factors in C. albicans
2.6. Effects of ASA, TE1, and TE2 on C. albicans via Addition of cAMP
3. Discussion
4. Materials and Methods
4.1. Chemicals, Strains, and Growth Conditions
4.2. Establishment of a Biofilm Model
4.3. The Effects of ASA, TE1, and TE2 on the Adhesion Ability of C. albicans ATCC 10231
4.4. The Effects of ASA, TE1, and TE2 on Biofilm of C. albicans ATCC 10231
4.5. Determination of Cell Surface Hydrophobicity (CSH)
4.6. Extracellular Phospholipase Assay
4.7. Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) of Virulence Factor Genes Related to C. albicans ATCC 10231
4.8. cAMP Rescue Test
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primer | Sequence |
---|---|
ACT1-F | TGACCGAAGCTCCAATGAATCC |
ACT1-R | CCGGTGGTTCTACCAGAAGAGT |
ALS1-F | CCTATGCCACCACTACCACTGT |
ALS1-R | AATCGGAGGTTGTGCTGTTGAC |
ALS3-F | CGCAACCACCACTACCATTACC |
ALS3-R | CACCTGGAGGAGCAGTGATTGT |
HWP1-F | ACAGGTAGACGGTCAAGGTGAA |
HWP1-R | TGAGGTGGATTGTCGCAAGGT |
EAP1-F | TGTGATGGCGGTTCTTGTTCTC |
EAP1-R | GTGGACTCGGTAGCTGGTGTAG |
NRG1-F | TGCTAGTGCTGCTGGTAGTACA |
NRG1-R | CTGCTGCTGCTTGGTTGGTATT |
UME6-F | TGGTGGTGTCAGTGTTAGTGCT |
UME6-R | TTGGTGGTGGTGGAAGAGAAGG |
HGC1-F | GCAACCACCACCACCAATGAA |
HGC1-R | ACAGCACGAGAACCAGCGATA |
EED1-F | TGCTCTACCACCACAACAAG |
EED1-R | TTGCGGTGCTTGCTCATA |
HYR1-F | GCTCAGGCTCAGGCTCACAA |
HYR1-R | TTCGGAACCAGAACCAGAACCA |
TPK2-F | TTCAACAACCGCAGCAACAACT |
TPK2-R | TTCAGCAGCCGATTTGGAAACA |
RAS1-F | TGGTGGTGTAAGTAGTGATGGA |
RAS1-R | GTTGTTGCTGTTGTTGTTGTCT |
EFG1-F | TTCACAACAGCCACCACTACCA |
EFG1-R | TGCTCTTCTGACAACCGACACA |
CPH1-F | TGCTGCCACTGCTCCAATGTA |
CPH1-R | TGCTGCTGCTGCTGTTGTTG |
SAP5-F | CCGTCGATGAGACTGGTAGAGA |
SAP5-R | GGGAAGTGCGGGAAGATGCT |
PLB1-F | TGGTCGTCCAACTGGTAAGTGT |
PLB1-R | GCCATCTTCTCCACCGTCAAC |
PLB2-F | ACATGCCAATCCCACCATTCCT |
PLB2-R | TGACCGTCTTCACCTCCATCTG |
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Chen, Y.; Gao, Y.; Li, Y.; Yin, J. Anti-Biofilm Activity of Assamsaponin A, Theasaponin E1, and Theasaponin E2 against Candida albicans. Int. J. Mol. Sci. 2024, 25, 3599. https://doi.org/10.3390/ijms25073599
Chen Y, Gao Y, Li Y, Yin J. Anti-Biofilm Activity of Assamsaponin A, Theasaponin E1, and Theasaponin E2 against Candida albicans. International Journal of Molecular Sciences. 2024; 25(7):3599. https://doi.org/10.3390/ijms25073599
Chicago/Turabian StyleChen, Yuhong, Ying Gao, Yifan Li, and Junfeng Yin. 2024. "Anti-Biofilm Activity of Assamsaponin A, Theasaponin E1, and Theasaponin E2 against Candida albicans" International Journal of Molecular Sciences 25, no. 7: 3599. https://doi.org/10.3390/ijms25073599