Novel Relationship between Mitofusin 2-Mediated Mitochondrial Hyperfusion, Metabolic Remodeling, and Glycolysis in Pulmonary Arterial Endothelial Cells
Abstract
:1. Introduction
2. Results
2.1. Overexpression of Mfn2 Induces Metabolic Reprogramming in Pulmonary Arterial Endothelial Cells
2.2. Mfn2 Overexpression Reprograms Cellular Energy Metabolism to Favor Glycolysis in Pulmonary Arterial Endothelial Cells
2.3. Mfn2 Overexpression Increases Mitochondrial (mt)-ROS and HIF-1α Levels in Pulmonary Arterial Endothelial Cells
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Animal and Ethics Statement
4.3. Cell Culture and Adenoviral Transduction of PAECs
4.4. Western Blot Analysis
4.5. Gas Chromatography–Mass Spectrometry (GC-MS) Metabolic Flux Assay
4.6. Metabolic Flux Analysis
4.7. Measurement of Oxygen Consumption Rate
4.8. Measurement of Extracellular Acidification Rate
4.9. Analysis of Real-Time Cellular ATP Rates
4.10. In Situ Apoptosis Assay
4.11. BrdU Cell Proliferation Assay
4.12. Analysis of Mitochondrial Fusion
4.13. Analysis of Mitochondrial (mt)-ROS and the Mitochondrial Membrane Potential
4.14. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yegambaram, M.; Sun, X.; Flores, A.G.; Lu, Q.; Soto, J.; Richards, J.; Aggarwal, S.; Wang, T.; Gu, H.; Fineman, J.R.; et al. Novel Relationship between Mitofusin 2-Mediated Mitochondrial Hyperfusion, Metabolic Remodeling, and Glycolysis in Pulmonary Arterial Endothelial Cells. Int. J. Mol. Sci. 2023, 24, 17533. https://doi.org/10.3390/ijms242417533
Yegambaram M, Sun X, Flores AG, Lu Q, Soto J, Richards J, Aggarwal S, Wang T, Gu H, Fineman JR, et al. Novel Relationship between Mitofusin 2-Mediated Mitochondrial Hyperfusion, Metabolic Remodeling, and Glycolysis in Pulmonary Arterial Endothelial Cells. International Journal of Molecular Sciences. 2023; 24(24):17533. https://doi.org/10.3390/ijms242417533
Chicago/Turabian StyleYegambaram, Manivannan, Xutong Sun, Alejandro Garcia Flores, Qing Lu, Jamie Soto, Jaime Richards, Saurabh Aggarwal, Ting Wang, Haiwei Gu, Jeffrey R. Fineman, and et al. 2023. "Novel Relationship between Mitofusin 2-Mediated Mitochondrial Hyperfusion, Metabolic Remodeling, and Glycolysis in Pulmonary Arterial Endothelial Cells" International Journal of Molecular Sciences 24, no. 24: 17533. https://doi.org/10.3390/ijms242417533