Alginate Oligosaccharides Repair Liver Injury by Improving Anti-Inflammatory Capacity in a Busulfan-Induced Mouse Model
Abstract
:1. Introduction
2. Results
2.1. AOS Repaired Busulfan-Induced Liver Injury
2.2. AOS Improved Busulfan-Disturbed Gene Expression in Liver
2.3. AOS Diminished Busulfan-Induced Inflammation Status in Liver
2.4. AOS Benefitted the Blood Metabolism to Support the Repair of Busulfan-Induced Liver Damage
3. Discussion
4. Materials and Methods
4.1. Study Design and Ethics
4.2. Ultrastructural Pathological Determination by Transmission Electron Microscope (TEM)
4.3. Gene Expression Determined by Quantitative Transcriptomics (RNA-Seq 168 Transcript Profiling) and Messenger RNA (mRNA) Quantitative Real-Time Polymerase Chain Reaction (q-RT-PCR)
4.3.1. RNA Sequencing (RNA-Seq) Analysis
4.3.2. mRNA q-RT-PCR Analysis
4.4. Histopathological Analysis
4.5. Western Blotting (WB)
4.6. Plasma Metabolite Measurements by LC/MS
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Genes | Sequences (5’–3’) | Fragment Size (bp) | Accession No. |
---|---|---|---|
β-Actin | F: TCGTGGGCCGCTCTAGGCAC | 255 | NM_007393.3 |
R: TGGCCTTAGGGTTCAGGGGGG | |||
GAPDH | F: AACAGCAACTCCCACTCTTC | 111 | NM_001289726.1 |
R: CCTGTTGCTGTAGCCGTATT | |||
IFNAR1 | F: CACGGTCGCTGTAGAAGTAAAG | 104 | NM_010508.2 |
R: TCTCCTCCTCTTCGTTGGAATA | |||
IFNAR2 | F: GACTTAAGAGCTGAGCAGGATG | 106 | NM_001110498.1 |
R: AGACGGTGTGATAGTCTCTAGG | |||
Tgfb3 | F: CGCTACATAGGTGGCAAGAA | 108 | NM_009368.3 |
R: CAAGTTGGACTCTCTCCTCAAC | |||
CD86 | F: CCTGGAAAGGTCTGGAGAATG | 110 | NM_019388.3 |
R: GGCAGATCAGTCCTTCCATAAA | |||
Tlr4 | F: GAGCAAACAGCAGAGGAAGA | 111 | NM_021297.3 |
R: CCAGGTGAGCTGTAGCATTTA | |||
Mrc1 | F: GGCGAGCATCAAGAGTAAAGA | 98 | NM_008625.2 |
R: CATAGGTCAGTCCCAACCAAA | |||
Ccl2 | F: GCTCAGCCAGATGCAGTTA | 104 | NM_011333.3 |
R: CTGCTGGTGATCCTCTTGTAG | |||
Egr1 | F: CGTCCTGTTCCCTTTGACTT | 122 | NM_007913.5 |
R: GCATGTGATGGAGAGGATACTG | |||
JUN | F: CCAGACTGTACACCAGAAGATG | 102 | NM_010591.2 |
R: CAACCAAAGTGTCTGCTTTCC | |||
Ifit1 | F: CAGGATATTCACCTCCGCTATG | 117 | NM_008331.3 |
R: CCTCCAAGCAAAGGACTTCT | |||
Tlr7 | F: GTACCAAGAGGCTGCAGATTAG | 135 | NM_001290755.1 |
R: CCTCAAGGCTCAGAAGATGTAAG | |||
Ifi44 | F: GCTGGGAAGTCTAGCTTTGT | 110 | NM_001370771.1 |
R: GTCCTGTACTTGTCAGAGATTCC |
Antibodies | Cat. No. | Company (accessed on 23 August 2020) |
---|---|---|
β-Actin | D110001-0200 | www.sangon.com.cn |
IFN-γ | bs-0481R | www.bioss.com.cn |
IL-6 | bs-0782R | www.bioss.com.cn |
IL-6R | bs-23660R | www.bioss.com.cn |
IL-22R | bs-2624R | www.bioss.com.cn |
TGF-β1 | bsm-33345M | www.bioss.com.cn |
TNF-α | bs-10802R | www.bioss.com.cn |
STAT3 | bs-55208R | www.bioss.com.cn |
TRIB3 | bs-7538R | www.bioss.com.cn |
GPX1 | bs-3882R | www.bioss.com.cn |
SOD1 | bs-10216R | www.bioss.com.cn |
IGF1R | bs-4985R | www.bioss.com.cn |
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Hao, Y.; Fang, H.; Yan, X.; Shen, W.; Liu, J.; Han, P.; Zhao, Y.; Zhang, W.; Feng, Y. Alginate Oligosaccharides Repair Liver Injury by Improving Anti-Inflammatory Capacity in a Busulfan-Induced Mouse Model. Int. J. Mol. Sci. 2023, 24, 3097. https://doi.org/10.3390/ijms24043097
Hao Y, Fang H, Yan X, Shen W, Liu J, Han P, Zhao Y, Zhang W, Feng Y. Alginate Oligosaccharides Repair Liver Injury by Improving Anti-Inflammatory Capacity in a Busulfan-Induced Mouse Model. International Journal of Molecular Sciences. 2023; 24(4):3097. https://doi.org/10.3390/ijms24043097
Chicago/Turabian StyleHao, Yanan, Hanhan Fang, Xiaowei Yan, Wei Shen, Jing Liu, Pengfei Han, Yong Zhao, Weidong Zhang, and Yanni Feng. 2023. "Alginate Oligosaccharides Repair Liver Injury by Improving Anti-Inflammatory Capacity in a Busulfan-Induced Mouse Model" International Journal of Molecular Sciences 24, no. 4: 3097. https://doi.org/10.3390/ijms24043097