EMD-57033 Augments the Contractility in Porcine Myocardium by Promoting the Activation of Myosin in Thick Filaments
Abstract
:1. Introduction
2. Results
2.1. Contractility of Muscle Tissue after the EMD Treatment
2.2. Alterations in the Biochemical States of the Myosin Heads with EMD Treatment
2.3. Changes in the X-ray Equatorial Diffraction Patterns with EMD
2.4. Changes in the Meridional X-ray Reflections and the Layer Lines with EMD
3. Discussion
3.1. EMD Recruits Myosin from the Biochemically-Defined SRX State
3.2. EMD Recruits Myosin from the Structurally-Defined OFF State
3.3. Sarcomeric Activators as an Approach for Rescuing the Contractility in Myocardium
4. Materials and Methods
4.1. Isometric Tension-Calcium Relationships
4.2. Myosin ATP Turnover Kinetics
4.3. Muscle Preparations for the Small-Angle X-Diffraction
4.4. Statistics
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Jani, V.; Qian, W.; Yuan, S.; Irving, T.; Ma, W. EMD-57033 Augments the Contractility in Porcine Myocardium by Promoting the Activation of Myosin in Thick Filaments. Int. J. Mol. Sci. 2022, 23, 14517. https://doi.org/10.3390/ijms232314517
Jani V, Qian W, Yuan S, Irving T, Ma W. EMD-57033 Augments the Contractility in Porcine Myocardium by Promoting the Activation of Myosin in Thick Filaments. International Journal of Molecular Sciences. 2022; 23(23):14517. https://doi.org/10.3390/ijms232314517
Chicago/Turabian StyleJani, Vivek, Wenjing Qian, Shengyao Yuan, Thomas Irving, and Weikang Ma. 2022. "EMD-57033 Augments the Contractility in Porcine Myocardium by Promoting the Activation of Myosin in Thick Filaments" International Journal of Molecular Sciences 23, no. 23: 14517. https://doi.org/10.3390/ijms232314517