The Distinct Role of the HDL Receptor SR-BI in Cholesterol Homeostasis of Human Placental Arterial and Venous Endothelial Cells
Abstract
:1. Introduction
2. Results
2.1. SR-BI Protein Is Elevated in ECA Compared to ECV
2.2. SR-BI Is Predominantly Expressed on the Apical Side of Placental Endothelial Cells
2.3. SR-BI Promoter Methylation Differs between ECA and ECV
2.4. GATA3 Transcription Factor Might Contribute to Differential SR-BI Expression
2.5. LXR Transcription Is Not Involved in SR-BI Regulation between Vascular Beds
2.6. PDZ Domain-Containing Protein PDZK1 Is Equally Expressed in ECA and ECV
2.7. Neither Oxygen nor Shear Stress Regulate SR-BI Protein in Primary Placental Endothelial Cells
2.8. Selective HDL CE-Uptake Is Impaired in ECV Compared to ECA
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Immunohistochemistry
4.3. Double-Fluorescence Microscopy
4.4. Light Microscopy
4.5. Isolation of Human Feto-Placental Endothelial Cells from Arteries and Veins
4.6. Cell Culture Experiments
4.7. Simulation of Shear Stress
4.8. Immunoblot Analyses
4.9. Quantitative RT-PCR
4.10. Genome-Scale DNA Methylation Analysis: Data Acquisition and Processing
4.11. HDL-Isolation and Labelling
4.12. HDL Lipid-Uptake
4.13. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strahlhofer-Augsten, M.; Schliefsteiner, C.; Cvitic, S.; George, M.; Lang-Olip, I.; Hirschmugl, B.; Marsche, G.; Lang, U.; Novakovic, B.; Saffery, R.; et al. The Distinct Role of the HDL Receptor SR-BI in Cholesterol Homeostasis of Human Placental Arterial and Venous Endothelial Cells. Int. J. Mol. Sci. 2022, 23, 5364. https://doi.org/10.3390/ijms23105364
Strahlhofer-Augsten M, Schliefsteiner C, Cvitic S, George M, Lang-Olip I, Hirschmugl B, Marsche G, Lang U, Novakovic B, Saffery R, et al. The Distinct Role of the HDL Receptor SR-BI in Cholesterol Homeostasis of Human Placental Arterial and Venous Endothelial Cells. International Journal of Molecular Sciences. 2022; 23(10):5364. https://doi.org/10.3390/ijms23105364
Chicago/Turabian StyleStrahlhofer-Augsten, Manuela, Carolin Schliefsteiner, Silvija Cvitic, Meekha George, Ingrid Lang-Olip, Birgit Hirschmugl, Gunther Marsche, Uwe Lang, Boris Novakovic, Richard Saffery, and et al. 2022. "The Distinct Role of the HDL Receptor SR-BI in Cholesterol Homeostasis of Human Placental Arterial and Venous Endothelial Cells" International Journal of Molecular Sciences 23, no. 10: 5364. https://doi.org/10.3390/ijms23105364