Cholic Acid-Based Antimicrobial Peptide Mimics as Antibacterial Agents

Abstract

There is a significant and urgent need for the development of novel antibacterial agents to tackle the increasing incidence of antibiotic resistance. Cholic acid-based small molecular antimicrobial peptide mimics are reported as potential new leads to treat bacterial infection. Here, we describe the design, synthesis and biological evaluation of cholic acid-based small molecular antimicrobial peptide mimics. The synthesis of cholic acid analogues involves the attachment of a hydrophobic moiety at the carboxyl terminal of the cholic acid scaffold, followed by the installation of one to three amino acid residues on the hydroxyl groups present on the cholic acid scaffold. Structure–activity relationship studies suggest that the tryptophan moiety is important for high antibacterial activity. Moreover, a minimum of +2 charge is also important for antimicrobial activity. In particular, analogues containing lysine-like residues showed the highest antibacterial potency against Gram-positive S. aureus. All di-substituted analogues possess high antimicrobial activity against both Gram-positive S. aureus as well as Gram-negative E. coli and P. aeruginosa. Analogues 17c and 17d with a combination of these features were found to be the most potent in this study. These compounds were able to depolarise the bacterial membrane, suggesting that they are potential antimicrobial pore forming agents.

1. Introduction

Treatment of bacterial infections is one of the most prevalent and challenging health issues faced today [1]. The evolutionary stress on a bacterial population exerted by the use of conventional antimicrobial agents induces antibiotics resistance [2]. Nowadays, infections caused by multi-drug resistant pathogens account for one of the leading causes of hospitalisation and increased mortality worldwide [3,4]. A team of European researchers estimated that over 33,000 people in Europe die each year as a direct consequence of an infection due to bacteria that are resistant to antibiotics [5]. A review commissioned by the government of the United Kingdom in 2014 estimated that the total of about 700,000 deaths occurring globally every year from drug-resistant bacterial infections could rise to 10 million each year by 2050 if corrective action is not taken [3].

Naturally occurring antimicrobial peptides (AMPs) have long been found to be ubiquitous and play a central role in the first line of defence in the innate immune system in all classes of organisms, ranging from prokaryotes to higher eukaryotes [6]. AMPs possess a broad spectrum activity against bacteria, viruses, parasites and fungi [6]. These AMPs adopt a wide range of secondary structures and non-conserved sequence homology, suggesting that antimicrobial activity is not dependent on any conserved amino acid sequences nor specific protein folding [7,8]. Instead, their facially amphiphilic character with hydrophobic residues clustered on one face of the helix and the cationic groups oriented on the opposite face appears to be essential for their antimicrobial properties [9]. As a result of this distinct structural property of AMPs, it is believed that AMPs act directly on the bacterial cell membrane through an initial electrostatic interaction between the cationic residues and the negatively charged cell membrane, followed by the membrane insertion via hydrophobic interactions between the hydrophobic residues and the fatty acid chains of the phospholipids, disrupting the bacterial cell membrane permeability.

Despite their desirable characteristics, AMPs have several drawbacks. Their relatively large size (ca. 20–50 amino acids) aggravates structural complexity and makes AMPs relatively difficult and expensive to synthesise on a large scale [8]. Another major challenge in the development of AMPs for clinical use is the lack of metabolic stability due to their susceptibility to proteolytic degradation [8,10]. Consequently, interest in antimicrobial strategies has shifted from AMPs to small molecular antimicrobial peptidomimetics (SMAMPs), which are non-peptidic small molecules that adopt the same mechanism of action as AMPs while circumventing the problems associated with high manufacturing costs and stability.

Many synthetic SMAMPs have been investigated using a variety of scaffolds to achieve structural amphiphilicity with several currently undergoing clinical trials [11,12]. For example, LTX-109 (Figure 1), a tripeptide composed of a modified tryptophan connected to guanidine and arginine with the end capped with a hydrophobic phenethylamino group, recently completed clinical phase II clinical trials for the treatment of nasal decolonisation of methicillin-resistant and -sensitive Staphylococcus aureus strains [13]. Given their improved properties over natural AMPs and low resistance potential, SMAMPs are a promising class for the further development of therapeutic antibacterial agents.

Previously, the Savage group introduced synthetic SMAMPs (e.g., 1) consisting of a sterol backbone appended with different cationic groups, which showed broad-spectrum activity against multi-drug resistant strains of Gram-positive and Gram-negative bacteria [14,15]. The facial amphiphilicity of the cholic-acid scaffold is attributed to the presence of hydroxyl groups at the concave face and the presence of the hydrocarbons at the convex face (Figure 2) [8]. In addition, the four fused rings provide a hydrophobic core that is large enough to allow spatial segregation between the hydrophobic and hydrophilic groups, further conferring the amphiphilic nature of the scaffold [10].

Many studies have highlighted the prevalence of tryptophan in potent naturally occurring AMPs, such as indolicidin and tritrpticin, and its importance in hydrophobic lipid binding has been widely recognised [16]. Our research group has also recently developed a potent, selective biphenyl-based peptidomimetic 2 (Figure 3), which incorporates the hydrophobic tryptophan motif to act as an antibacterial agent against both Gram-positive S. aureus and Gram-negative Escherichia coli [17]. Based upon the observations made in these studies, it was envisaged that the cholic acid scaffolds (lithocholic acid (LICA) 3a, chenodeoxycholic acid (CHCA) 3b, deoxycholic acid (DCA) 3c and cholic acid (CA) 3d) could be used to generate SMAMPs by incorporating a tryptophan moiety at the carboxyl terminal and cationic amino acid residues at the hydroxyl groups of the molecule. These SMAMPs would allow us to further elucidate the role of tryptophan in membrane permeability and to explore the impact that total net charge has on biological activity.

2. Results and Discussion

2.1. Synthesis of Cholic Acid-Based Antimicrobial Peptide Mimics

The cholic acid scaffolds (lithocholic acid 3a, chenodeoxycholic acid 3b, deoxycholic acid 3c, cholic acid 3d) (Figure 3) that varied in the number and position of the hydroxyl groups were chosen as starting materials to generate a library of facially amphiphilic SMAMPs by incorporating a hydrophobic moiety at the carboxyl terminal and cationic amino acid residues at the hydroxyl groups of the starting scaffolds. To achieve this, the carboxyl terminus of the cholic acid derivatives 3a–3d were firstly functionalised with tryptophan methyl ester via N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) coupling using the method reported by Gomez-Mendoza et al. in 70–93% yield (Scheme 1) [18]. Similarly, phenylalanine methyl ester conjugated chenodeoxycholic acid derivative 8 was synthesised from chenodeoxycholic acid 3c and L-phenylalanine methyl ester hydrochloride 6 in 88% yield (Scheme 2). The aromatic benzylamine 7 was also conjugated to chenodeoxycholic acid 3c using similar reaction conditions to result in the benzylamine derivative 9 in 60% yield (Scheme 2).

Following the successful coupling of the hydrophobic group to the carboxyl terminal of the cholic acid scaffolds, the cationic amino groups were tethered to the hydroxyl termini. This was achieved via ester bond formation using Steglich esterification conditions, following the procedure described by Yadav et al. (Scheme 3) [19]. Amino acids such as glycine (Gly), γ-aminobutyric acid (GABA), ornithine (Orn) or lysine (Lys) were chosen to provide the charged characteristic in order to investigate the effect of total net charge on antibacterial activity. Moreover, the number of ester bonds installed was dependent on the number of hydroxyl groups present on the starting scaffold. Thus, the Boc-protected mono-, di- and tri- amino derived cholic acid intermediates were firstly synthesised in 10–76% yield. Further treatment with 4 M hydrogen chloride/dioxane solution in dichloromethane at room temperature for 2–4 h resulted in the formation of desired mono-, di- and tri- substituted cholic acid based SMAMPs in 67–96% yield (Scheme 3). The variable yield of the SMAMPs product could be due to the loss of product when washing the crude product with diethyl ether. The purity of the SMAMPs was estimated from NMR spectra and was judged to be >95% (see Supplementary Materials File S1).

To investigate the effect of specific amino functionality, a terminal guanidine was prepared from deprotected GABA monoester 16b and N,N′-di-Boc-1H-pyrazole-1-carboxamidine 22 in the presence of basic triethylamine at room temperature (Scheme 4), followed by the removal of Boc protecting groups with trifluoroacetic acid (TFA) to give analogue 23 in a yield of 53% over two steps, following the method reported by Yu et al. [20,21].

In this study, two dipeptide cholic acid analogues, 24 and 26, were also synthesised to investigate the effect of chain length and functionality of the cationic residues on the antibacterial activity. In particular, analogue 26 was designed in accordance with the previously reported most active glycine-valine derivative by Yadav et al. [19]. Dipeptide derivatives were synthesised by two different reaction pathways, namely amine-reactive crosslinker reactive groups, or carboxyl-reactive chemical groups.

For the synthesis of analogue 25, the glycine terminal of the deprotected lithocholic acid derivative 16a was conjugated with a lysine residue. This was achieved by treating analogue 16a with N_ε-Z-N_α-Boc-L-lysine hydroxysuccinimide ester 24 in the presence of DIPEA in room temperature overnight to afford the N-Boc-protected glycine–lysine dipeptide analogues. The Boc-protected product was then deprotected using 4 M hydrogen chloride/dioxane solution to afford the dipeptide analogue 25 in a yield of 15% over two steps (Scheme 5).

Conversely, a glycine–valine dipeptide analogue 27 was synthesised via EDC coupling following the procedure reported by Yadav et al. [19] N-Boc-protected valine 26 was stirred with glycine triester derivative 19a, EDC.HCl, 1-hydroxybenzotriazole (HOBt) hydrate and DIPEA in anhydrous dichloromethane at room temperature for 48 h to yield the Boc-protected dipeptide cholic acid derivative. The deprotection of the Boc groups was performed using 4 M HCl in dioxane to afford the Gly–Val dipeptide analogue 27 in a yield of 8% over two steps (Scheme 6).

2.2. Structure–Activity Relationship Study

The antibacterial activity of each compound was measured as the minimum inhibitory concentration (MIC, i.e., the lowest concentration of the compound needed to inhibit the growth of bacterial cells) following a previously published protocol [22]. The compounds were tested in a concentration range between 1 and 125 μM against Gram-positive S. aureus as well as Gram-negative E. coli and Pseudomonas aeruginosa. MIC values for tested compounds ranged from 4 μM to greater than 125 μM against S. aureus (

Table 1. Minimum inhibitory concentration of compounds against S. aureus and E. coli. (ND = Not determined).

Compound				MIC (μM)
	Scaffold	Cationic Group	Carboxyl Terminal Moiety	S. Aureus	S. Aureus	S. Aureus	E. Coli	P. Aeruginosa (PAO1)
				(SA38)	(SA111)	(SA112)	(K12)
16a	LICA	Gly	Trp OMe	>125	ND	ND	>125	>125
16b	LICA	GABA	Trp OMe	>125	ND	ND	>125	>125
16c	LICA	Orn	Trp OMe	8	8	4	>125	>125
16d	LICA	Lys	Trp OMe	4	8	8	>125	>125
17a	CHCA	Gly	Trp OMe	8	8	8	16	>125
17b	CHCA	GABA	Trp OMe	16	ND	ND	4	16
17c	CHCA	Orn	Trp OMe	4	4	4	16	8
17d	CHCA	Lys	Trp OMe	4	4	4	16	8
18a	DCA	Gly	Trp OMe	8	8	8	16	>125
19a	CA	Gly	Trp OMe	31	ND	ND	31	>125
19b	CA	GABA	Trp OMe	16	ND	ND	16	31
19c	CA	Orn	Trp OMe	16	ND	ND	4	16
19d	CA	Lys	Trp OMe	16	ND	ND	31	16
20	CHCA	Orn	Phe OMe	8	8	8	16	31
21	CHCA	Orn	NHBn	8	8	8	16	31
23	LICA	Guanidine–GABA	Trp OMe	16	ND	ND	>125	>125
25	LICA	Gly–Lys	Trp OMe	8	4	4	>125	>125
27	CA	Gly–Val	Trp OMe	16	ND	ND	8	63

). The most active compounds against the Gram-positive S. aureus SA38 strain were 16d, 17c and 17d, sharing a MIC value of 4 μM. A selection of compounds that had high antibacterial activity (with MIC values of 4 or 8 μM) against the Gram-positive S. aureus SA38 strain were also tested against antibiotic-resistant S. aureus SA111 and SA112 strains [23]. These compounds showed similar MIC values of 4 or 8 μM against the antibiotic-resistant S. aureus SA111 and SA112 strains compared to S. aureus SA38 strain. Across all three tested S. aureus strains, compounds 17c and 17d were the most active compounds, with MIC value of 4 μM against all three S. aureus strains.

Against Gram-negative E. coli and P. aeruginosa, the tested compounds generally showed lower antibacterial activities with higher MIC values compared to Gram-positive S. aureus, except compounds 17b, 19c and 27, which had lower MIC values of 4, 4 and 8 μM, respectively, against E. coli and were the most active compounds against E. coli. On the other hand, compounds 17c and 17d were the most active compounds against P. aeruginosa, sharing a MIC value of 8 μM.

2.2.1. Effect of Total Net Charge

Positively charged groups are one of the most important features for non-receptor mediated membrane antimicrobial agents, due to the subtle difference in membrane composition between prokaryotic and eukaryotic cell membranes [6,24]. A number of studies had suggested that the number of total net charge played a crucial role in describing the antimicrobial activities of AMPs and their peptidomimetics against bacterial membranes [24]. Many reported higher positive charges were correlated with enhanced bactericidal effect [25,26]. In this study, analogues where the number of positively charged residues varied from 1 to 3 were synthesised, and the number of net charges of these analogues varied between +1 and +6. Against Gram-positive S. aureus SA38 strain, increasing the net charge of analogues from +1 to +2 resulted in greater than 32-fold increase in antimicrobial activity (i.e., 16a (>250 μM) to 17a (8 μM)). Meanwhile, the activity remained largely unchanged when the net charge of the analogues was further increased from +2 to +4, with a slight increase in activity observed between 16c and 17c, while no difference was observed between 16d and 17d against Gram-positive S. aureus SA38 strain. Analogues bearing three cationic residues (with net charge of +3 or +6) resulted in either equal (e.g., 17b and 19b) or a four-fold reduction in antimicrobial activity (e.g., 17a and 19a) against Gram-positive S. aureus SA38 strain. This trend was also observed in the antibacterial activities of compounds against Gram-negative P. aeruginosa, as compounds 17c and 17d with the optimum net charge of +4 shard a MIC value of 8 μM against Gram-negative P. aeruginosa and were the most potent compounds against Gram-negative P. aeruginosa. Compounds with a net charge of above or below the optimum value of +4 showed lower antibacterial activity against Gram-negative P. aeruginosa. Overall, the di-substituted cholic acid derivatives 17c and 17d, each bearing two cationic amino acid groups with an overall net charge of +4, were optimal for antibacterial activity.

2.2.2. Effect of the Tryptophan

In this study, the tryptophan derivative at the carboxyl terminal was modified to further elucidate the significance of tryptophan in antimicrobial activity. Replacement of tryptophan methyl ester with a phenylalanine methyl ester (20) or a benzylamine (21) both resulted in a two-fold decrease in antimicrobial activity against all tested Gram-positive S. aureus strains compared to tryptophan-containing derivative 17c, as suggested by an increase in MIC value from 4 μM for compound 17c to 8 μM for compounds 20 and 21. A similar trend was also observed against Gram-negative P. aeruginosa, as tryptophan-containing compound 17c with MIC value of 8 μM was about four times more active than phenylalanine-containing compound 20 and benzylamine 21, which had MIC value of 31 μM against P. aeruginosa. However, activity against Gram-negative E. coli was maintained after the replacement of the tryptophan methyl ester residue (17c). This result indicated that the tryptophan residue is not essential for activity against Gram-negative E. coli. Indeed, analogue 27 bearing a tryptophan methyl ester and a previously reported literature compound with a benzyl residue at the carboxyl terminal of cholic acid in place of the tryptophan methyl ester moiety showed the same antibacterial activity of 8 μM against Gram-negative E. coli [19]. While tryptophan, phenylalanine and benzylamine are all hydrophobic and aromatic, tryptophan constitutes a unique biochemistry with characteristic indole functionality. The hydrophobic aromatic ring has a strong tendency to facilitate the insertion of the molecule into the biological membrane, however, the presence of an indole side chain allows hydrogen bonding, also giving a hydrophilic character. Hence, tryptophan containing amphiphiles has a stronger preference for the membrane-water interfacial regions of lipid bilayers [16,27]. Theoretically, non-tryptophan counterparts may promote deeper penetration into the membrane due to their high hydrophobicity [28]. However, this trend was not aligned with the actual MICs values, suggesting that the formation of membrane-spanning pores is unlikely. The disparity in activities between the Gram-negative and Gram-positive bacterial strains could be explained by the presence of the lipopolysaccharide outer cell membrane external to the peptidoglycan cell wall present only in Gram-negative bacteria, suggesting a different mode of action between Gram-positive and Gram-negative strains. Gram-negative bacteria have a stronger efflux system that recognise noxious compounds and act as a transporter to excrete compounds from within the bacterial cell [29].

2.2.3. Presence of Specific Amino Acid Residues

Despite the significant role played by hydrophobic tryptophan amino acids in membrane permeability, cholic acid scaffold encompassing cationic amino acid residues with different length and charges were also synthesised to investigate the role of specific amino acids in relation to antibacterial activity. Analogues 16c, 16d and 23 containing ornithine, lysine or guanidine were identified to be the active compounds in the mono-substituted lithocholic acid-based scaffold against Gram-positive S. aureus SA38 strain. Among these analogues, the guanidine bearing compound 23 was least active, with the highest MIC value of 16 μM, and the lysine substituted analogue 16d was the most active, with the lowest MIC value of 4 μM. Ornithine is a non-proteinogenic cationic amino acid that retains the primary amino groups of lysine, but it is one carbon shorter than lysine. However, replacement of lysine (16d) with ornithine (16c) resulted in a two-fold reduction in activity. The two-fold reduction in activity was also observed in the replacement of lysine with a dipeptide variant containing glycine and lysine residues (25). These findings, therefore, suggested that while cationic charges are essential for antibacterial activity, deviation from the optimum cationic chain length and specific hydrophobicity window would result in the reduction in antimicrobial activity.

2.2.4. Effect of the Position and Number of the Cationic Groups

In this study, analogues 17a and 18a were synthesised to investigate whether a change in the position of the cationic groups on the cholic acid scaffold would influence their antimicrobial activities. Analogues 17a and 18a differ in the sites of cationic glycine amino side chains, with the second glycine side chains attached at either C7-OH or C12-OH of the tryptophan conjugated cholic acid scaffold. Equal activities of 8, 16 and >125 μM against all tested Gram-positive S. aureus strains, Gram-negative E. coli and P. aeruginosa for both analogues suggested that the antimicrobial mechanism was not dependent on the position of the substituted amine for the di-substituted cholic acid-based antimicrobial agents. However, attaching three cationic residues exhibited a reduction in activities against all tested strains, with an exception for analogue 19b when compared with corresponding di-substituted compound 17b against Gram-positive S. aureus SA38 strain and analogue 19c when compared with corresponding di-substituted compound 17c against Gram-negative E. coli. The reduction in activities could be explained by the repulsive interactions between the positive charged groups. In the case of 19a, the shorter chain length of glycine residues may have resulted in the three repulsive charges being within closer proximity to one another, potentially negatively affecting the conformation of compound and its interaction with the bacterial cell membrane. Reduction in activities could also be due to a strong interaction between the cationic charges and the bacterial negatively charged headgroups. In the case of 19c and 19d, a total charge of +6 facilitates a much stronger electrostatic binding with the negatively charged membrane, that could potentially restrain deeper penetration of the AMP mimic into the inner leaflet of the membrane by a single hydrophobic tryptophan moiety.

2.3. Membrane Conduction

Tethered bilayer lipid membranes (tBLMs), in association with AC electrical impedance spectroscopy techniques, were employed to measure changes in lipid bilayer conductance and capacitance in the presence of selected potent compounds (16d, 17c, 17d, 19d, 20, 21, 27) [30,31]. This experiment involved the use of negatively charged POPG lipids, which mimic the negatively-charged bacterial cell membrane. The responses to these selected compounds in terms of altering membrane conduction only became apparent at concentrations as high as ~50 µM (Figure 4A). Deeper penetration into the membrane due to high hydrophobicity facilitated deeper penetration was supported by non-tryptophan analogues 20 and 21, as higher conduction changes were observed in negatively charged tBLMs. These responses can be considered small when compared to known pore-forming peptides such as PGLa when used on similar tBLM architecture, suggesting a transmembrane orientation described according to the barrel-stave model is less likely. Similarly, the relative responses in negatively charged tBLMs are modest in terms of capacitance changes (Figure 4B). Typically, increases in membrane capacitance indicate the tBLM is getting thinner, or there is an introduction of more water to the membrane. These data do suggest that these compounds are attracted to negatively charged lipid, like those that might be found in many bacterial membranes. The induction of increased overall membrane conduction with very small changes in membrane capacitance demonstrated that these compounds preferentially induce the formation of toroidal pore-like structures in membranes containing negatively charged lipids, either by themselves or by modulating the intrinsic pre-existing membrane pores via altering the critical packing parameter of the lipid bilayer. One molecule in particular, 17c, had a significant impact on membrane capacitance, suggesting a more membrane lytic effect (Figure 4B).

2.4. Cytoplasmic Membrane Depolarisation

The mechanisms of actions of selected potent compounds (16d, 17d, 20, 21, 27) were further investigated using a membrane dye release assay on the cytoplasmic membrane of S. aureus. Firstly, 3,3′-dipropylthiadicarbocyanine iodine (diSC3-5) is a cationic membrane potential-sensitive fluorescent dye that can readily penetrate and accumulate in the polarised bacterial lipid bilayers [17,32]. This accumulation results in self-quenching of the overall level of fluorescence. The distribution of the membrane potential-sensitive dye between the cell membrane and periphery medium is dependent on the cytoplasmic membrane potential gradient. However, if an antimicrobial compound perturbs the bacterial cell membrane, the membrane potential gradient can be compromised, causing a rapid release of dye into the medium, resulting in dequenching. Therefore, an increase in the fluorescence intensity of the dye indicates membrane depolarisation. As shown in Figure 5, all tested compounds induced disruption of the cytoplasmic membrane of Gram-positive S. aureus in a time-dependent manner at four times (4×) of their MIC. The level of increase in fluorescence intensity was observed to be correlated with antimicrobial activity, with compounds 16d and 17d having lower MIC values showing greater increases in fluorescence intensity than compounds 20, 21 and 27 with higher MIC values. Amongst these tested compounds, compound 17d was the most membrane active compound, as it induced the highest increase in fluorescence at 4× of its MIC at 15 min.

Time-kill kinetic assay was also carried out to further investigate the bactericidal mechanism of the active compound 17d (Figure 6). In this assay, S. aureus bacterial solution was treated with compound 17d at 1×, 2× and 4× of its MIC, and the amount of viable bacterial cells was quantified over time. The bacterial cell viability upon the treatment of compound 17d was time dependent and concentration dependent, with about 0.5-log reduction in viable bacteria at 4× of its MIC at 30 min. At lower concentrations of 1× and 2× of its MIC, the reductions in viable bacterial were modest.

Overall, these observations suggest that the tested compounds could act via permeabilisation of the bacterial cell membrane of S. aureus, which in turn causes cell death. However, these compounds could also exert their mechanism of action on various intracellular targets, and these will be investigated in future studies [33].

2.5. Cytotoxicity

The in vitro cytotoxicity of selected active compounds 16d and 17d was evaluated against MRC-5 normal human lung fibroblasts using the MTT assay at a concentration range of 1–500 μM. A dose–response curve was generated for each compound and the concentration of antimicrobial compound that kills 50% of the cell population (IC₅₀) was determined. The selectivity ratio as the IC₅₀ value divided by MIC value for each compound was then calculated (

Table 2. IC₅₀ value of compounds against MRC-5 normal human lung fibroblasts and their therapeutic indices with respect to S. aureus.

Compound	IC50 (µM)	MIC against S. Aureus (µM)	Therapeutic Window
16d	4.79	4	1.24
17d	33.3	4	8.32

). A poor selectivity ratio of 1.24 was observed in compound 16d, while 17d obtained a higher selectivity ratio of 8.32. Compounds 16d and 17d varied in the number of lysine residues, and therefore an increase in the number of total net charges, showing a greater selectivity against bacterial cells. Whilst a higher selectivity from the scaffold is desirable, this result indicates a potential means of improving the selectivity ratio. The cytotoxicity results, along with the broad-spectrum activity of di-substituted cholic acid-based analogues, suggest that the scaffold is a promising candidate for the further development of antimicrobial drugs to treat human bacterial infection.

3. Materials and Methods

3.1. Synthesis of Analogues

All commercially available reagents were purchased from standard suppliers (Sigma Aldrich, St Louis, MO, USA and Alfa-Aesar, Ward Hill, MA, USA) and were used without further purification. All reactions were performed under anhydrous conditions with an atmosphere of nitrogen and anhydrous solvent (as required). Anhydrous solvents were obtained using PureSolv MD Solvent Purification System. Reactions were monitored by thin-layer chromatography precoated with Merck silica gel 60 F254 and visualization was performed by using short or long wavelength of ultraviolet light. Flash chromatography was performed using Grace Davisil LC60A silica.

Melting points were measured using an OptiMelt melting point apparatus and were uncorrected. ¹H and ¹³C NMR spectra were obtained in the specified solvents on a Bruker Avance III 300, Bruker Avance III HD 400 or Bruker Avance III 600 Cryo spectrometer. Chemical shifts (δ) are in parts per million (ppm), internally referenced to the solvent nuclei. Multiplicities are assigned as singlet (s), broad singlet (bs), doublet (d), triplet (t), quartet (q), quintet (quint), sextet (sext), septet (sept), multiplet (m) or a combination of these (e.g., dd, dt, td), and coupling constants (J) are described in Hertz (Hz). Infrared (IR) spectra were recorded using a Cary 630 FTIR spectrometer fitted with a diamond attenuated total reflectance (ATR) sample interface. Low-resolution mass spectrometry was performed using a Thermo Fisher LCQ Mass Spectrometer while high-resolution mass spectrometry (HRMS) was performed by the UNSW Bioanalytical Mass Spectrometry Facility using a Thermo LTQ Orbitrap XL instrument.

3.1.1. General Synthetic Procedure A for Coupling of Hydrophobic Groups

To a solution of appropriate cholic acid derivative (1.0 equivalent) in DMF (3 mL), EDC.HCl (1.1 equivalent) and DIPEA (2.0 equivalents) were added portion-wise at room temperature under nitrogen atmosphere. A solution of L-tryptophan methyl ester hydrochloride, L-phenylalanine methyl ester hydrochloride or benzylamine (1.5 equivalents) and DMAP (0.3 equivalent) in DMF (2 mL) was then added dropwise to the reaction mixture at 0 °C. The reaction mixture was stirred at room temperature overnight. After the completion of the reaction, the reaction mixture was poured into water (50 mL). The white precipitant was collected via vacuum filtration, redissolved in DCM (25 mL) and washed with NaHCO₃ (2 × 50 mL), 2 M HCl (2 × 50 mL) and, finally, brine. The organic phase was then dried over anhydrous sodium sulfate and concentrated in vacuo to afford the product.

3.1.2. General Synthetic Procedure B for N-Boc-Protected Esters

To a solution of substituted cholic acid derivatives (1 equivalent) in DCM (5 mL), EDC.HCl (1.5–8 equivalents), Boc-protected amino acids (1.5–8 equivalents) and DMAP (1.5–8 equivalents) was added portion-wise at room temperature under nitrogen atmosphere. The resulting suspension was stirred at room temperature for 16–96 h. After completion of reaction, water (100 mL) was added to the reaction mixture. The organic layer was extracted with DCM (3 × 25 mL), washed with brine (50 mL), dried over anhydrous sodium sulfate and concentrated in vacuo to provide the crude product. The crude product was then purified by flash column chromatography on silica using ethyl acetate/chloroform (25:75) as eluents to afford the N-Boc-protected esters.

3.1.3. General Synthetic Procedure C for HCl Salts

To the Boc-protected product in DCM (10 mL), 4 M HCl in dioxane (3 mL) was added and the reaction mixture was stirred at room temperature for 2–4 h. After completion of reaction, the reaction mixture was concentrated in vacuo, triturated with diethyl ether and freeze-dried to afford the product as a HCl salt.

3.1.4. General Synthetic Procedure D for Guanidinium Salt Compound

To a solution of GABA-substituted lithocholic ester (1.0 equivalent) and N,N′-di-Boc-1H-pyrazole-1-carboxamidine (1.3 equivalents) in anhydrous DMF (2 mL), triethylamine (2.5 equivalents) was added dropwise at 0 °C under nitrogen atmosphere. The reaction mixture was stirred at room temperature overnight. After the completion of the reaction, the reaction mixture was added into an ice-water mixture (50 mL) and the precipitate formed was collected by vacuum filtration to provide the crude product. The crude was then purified by flash column chromatography on silica using ethyl acetate/n-hexane (20–50%) to afford the Boc-protected product. To the Boc-protected product in DCM (5 mL), TFA (5 mL) was added at 0 °C. The reaction was stirred at room temperature for 3 h. After completion of reaction, the reaction mixture was concentrated in vacuo, triturated with diethyl ether and freeze-dried to afford the product as a TFA salt.

3.1.5. General Synthetic Procedure E for Glycine-Lysine Monoester

To a solution of glycine-substituted lithocholic ester (1.0 equivalent) in DMF (3 mL), N,N′-di-Boc-L-lysine hydroxysuccinimide ester (1.2 equivalents) and DIPEA (4.0 equivalents) was added dropwise at 0 °C under nitrogen atmosphere. The reaction mixture was then stirred at room temperature overnight. After the completion of reaction, the reaction mixture was added to water (50 mL) and the white precipitant formed was collected via vacuum filtration, redissolved in DCM (25 mL), washed with brine (50 mL), dried over anhydrous sodium sulfate and concentrated in vacuo to provide the crude product. The crude product was purified by flash column chromatography on silica using ethyl acetate/n-hexane (25:75) to yield the Boc-protected product. To the Boc-protected product in DCM (10 mL), 4 M HCl in dioxane (3 mL) was added and stirred at room temperature for 4 h. After completion of reaction, the reaction mixture was concentrated in vacuo, triturated with diethyl ether and freeze-dried to afford the product as a HCl salt.

3.1.6. General Synthetic Procedure F for Glycine-Valine Dipeptide

To a solution of glycine-substituted cholic acid triester (1.0 equivalent) in anhydrous DCM (3 mL), Boc-Val-OH (6.0 equivalents), EDC.HCl (6.0 equivalent), HOBt (3.0 equivalents) and DIPEA (5.0 equivalents) was added portion-wise at 0 °C under nitrogen atmosphere. The reaction was stirred at room temperature for 48 h. After the completion of the reaction, water (100 mL) was added to the reaction mixture and the product was extracted into DCM (3 × 25 mL), washed with saturated NaHCO₃ (50 mL), 2 M HCl (50 mL) and brine (50 mL), dried over anhydrous sodium sulfate and concentrated in vacuo to give the crude product. The crude was purified by flash chromatography on silica using methanol/DCM (3%) as eluent to afford the Boc-protected product. To the Boc-protected product in DCM (10 mL), 4 M HCl in dioxane (3 mL) was added and stirred at room temperature for 4 h. After completion of the reaction, the reaction mixture was concentrated in vacuo, triturated with diethyl ether and freeze-dried to afford the product as a HCl salt.

3.1.7. 2-(((3R,5R,8R,9S,10S,13R,14S,17R)-17-((R)-5-(((S)-3-(1H-Indol-3-yl)-1-methoxy-1-oxopropan-2-yl)amino)-5-oxopentan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[α]phenanthren-3-yl)oxy)-2-oxoethan-1-aminium chloride (16a)

The title compound was synthesised from 10a (0.300 g, 0.458 mmol) following general synthetic procedure C. The product 16a was obtained as a white solid (0.195 g, 71%); mp 228.1 °C; ¹H NMR (400 MHz, DMSO-d₆) δ 0.60 (s, 3H, CH₃), 0.86 (d, J = 6.4 Hz, 3H, CH₃), 0.91 (s, 3H, CH₃), 0.96–3.13 (m, CH, CH₂ steroid), 2.97–3.18 (m, 2H, -CO-CH-CH₂), 3.57 (s, 3H, OCH₃), 3.76 (q, J = 5.6 Hz, 2H, -CO-CH₂-NH₃), 4.48 (td, J = 8.1, 5.8 Hz, 1H, -CO-CH-NH), 4.76 (tt, J = 11.1, 4.7 Hz, 1H, OCH), 6.98 (ddd, J = 8.0, 6.9, 1.1 Hz, 1H, ArH), 7.07 (ddd, J = 8.1, 6.9, 1.2 Hz, 1H, ArH), 7.14 (d, J = 2.4 Hz, 1H, ArH), 7.34 (d, J = 8.1 Hz, 1H, ArH), 7.49 (d, J = 7.8 Hz, 1H, ArH), 8.25 (d, J = 7.5 Hz, 1H, NH), 8.38 (d, J = 5.8 Hz, 3H, NH₃), 10.91 (d, J = 2.4 Hz, 1H, NH-indole); ¹³C NMR (100 MHz, DMSO-d₆) δ 173.2, 173.1, 167.5, 136.6, 127.5, 124.1, 121.4, 118.8, 118.5, 111.9, 110.1, 76.1, 66.8, 56.4, 56.1, 53.6, 52.2, 42.7, 41.5, 40.0, 39.8, 35.8, 35.4, 34.8, 34.6, 32.5, 32.2, 31.8, 28.2, 27.5, 27.0, 26.6, 26.4, 24.3, 23.4, 20.9, 18.8, 12.3; ν_max 3389, 2930, 2863, 2627, 2343, 1734, 1519, 1439, 1369, 1119, 1044, 973, 910, 745 cm⁻¹; HRMS (+ESI): Found m/z 634.4214, [M]⁺, C₃₈H₅₆N₃O₅ required 634.4215.

3.1.8. 4-(((3R,5R,8R,9S,10S,13R,14S,17R)-17-((R)-5-(((S)-3-(1H-Indol-3-yl)-1-methoxy-1-oxopropan-2-yl)amino)-5-oxopentan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[α]phenanthren-3-yl)oxy)-4-oxobutan-1-aminium chloride (16b)

The title compound was synthesised from 10b (0.273 g, 0.358 mmol) following general synthetic procedure C. The product 16b was obtained as a white solid (0.198 g, 79%); mp 161.3 °C; ¹H NMR (400 MHz, DMSO-d₆) δ 0.60 (s, 3H, CH₃), 0.86 (d, J = 6.4 Hz, 3H, CH₃), 0.91 (s, 3H, CH₃), 0.95–2.79 (m, CH, CH₂, steroid), 3.02–3.14 (m, 2H, -CO-CH-CH₂), 3.57 (s, 3H, OCH₃), 4.48 (td, J = 8.1, 5.8 Hz, 1H, -CO-CH-NH), 4.65 (td, J = 11.3, 10.6, 4.9 Hz, 1H, -O-CH), 6.98 (ddd, J = 8.0, 7.0, 1.1 Hz, 1H, ArH), 7.07 (ddd, J = 8.1, 6.9, 1.2 Hz, 1H, ArH), 7.14 (d, J = 2.4 Hz, 1H, ArH), 7.34 (dt, J = 8.1, 0.9 Hz, 1H, ArH), 7.49 (d, J = 7.8 Hz, 1H, ArH), 8.00 (s, 3H, NH₃), 8.24 (d, J = 7.6 Hz, 1H, NH), 10.90 (d, J = 2.5 Hz, 1H, NH-indole); ¹³C NMR (100 MHz, DMSO-d₆) δ 173.2, 173.1 170.2, 136.6, 127.5, 124.1, 121.4, 118.8, 118.5, 111.9, 110.1, 74.8, 56.3, 56.1, 55.4, 53.6, 52.2, 42.7, 41.7, 40.0, 35.8, 35.3, 35.1, 34.9, 34.6, 32.5, 32.3, 32.1, 31.8, 31.0, 28.2, 27.3, 27.1, 26.7, 26.4, 24.3, 23.5, 20.9, 18.8, 12.3; ν_max 3390, 2928, 2863, 2321, 1934, 1721, 1665, 1521, 1440, 1356, 1294, 1204, 1118, 1020, 858 cm⁻¹; HRMS (+ESI): Found m/z 662.4527, [M]⁺, C₄₀H₆₀N₃O₅ required 663.4528.

3.1.9. (S)-5-(((3R,5R,8R,9S,10S,13R,14S,17R)-17-((R)-5-(((S)-3-(1H-Indol-3-yl)-1-methoxy-1-oxopropan-2-yl)amino)-5-oxopentan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[α]phenanthren-3-yl)oxy)-5-oxopentane-1,4-diaminium chloride (16c)

The title compound was synthesised from 10c (0.350 g, 0.393 mmol) following general synthetic procedure C. The product 16c was obtained as a white solid (0.180 g, 67%); mp 185.7 °C; ¹H NMR (400 MHz, DMSO-d₆) δ 0.60 (s, 3H, CH₃), 0.86 (d, J = 6.3 Hz, 3H, CH₃), 0.92 (s, 3H, CH₃), 0.97–2.79 (m, CH, CH₂, steroid), 2.99–3.17 (m, 2H, -CO-CH-CH₂), 3.57 (s, 3H, OCH₃), 3.97 (t, J = 6.3 Hz, 1H, -CO-CH-NH₃), 4.48 (td, J = 8.1, 5.7 Hz, 1H, -CO-CH-CH₂), 4.75 (tt, J = 11.2, 4.6 Hz, 1H, OCH), 6.98 (t, J = 7.4 Hz, 1H, ArH), 7.06 (t, J = 7.5 Hz, 1H, ArH), 7.14 (d, J = 2.3 Hz, 1H, ArH), 7.34 (d, J = 8.1 Hz, 1H, ArH), 7.48 (d, J = 7.8 Hz, 1H, ArH), 8.25 (s, 1H, NH), 8.33 (m, 6H, NH), 10.93 (d, J = 2.4 Hz, 1H, NH-indole); ¹³C NMR (100 MHz, DMSO-d₆) δ 173.2, 173.1, 169.3, 136.6, 127.5, 124.1, 121.4, 118.8, 118.4, 111.9, 110.0, 76.3, 56.4, 56.1, 53.6, 52.2, 52.2, 51.9, 42.7, 41.6, 40.2, 38.5, 35.8, 35.4, 34.8, 34.6, 32.5, 32.2, 31.8, 28.2, 27.7, 27.5, 27.1, 26.6, 26.5, 24.3, 23.4, 23.1, 20.9, 18.8, 12.3; IR (ATR): ν_max 3389, 2929, 2861, 2647, 2320, 2192, 2087, 1869, 1729, 1664, 1521, 1456, 1356, 1231, 1775, 1097, 933, 860 cm⁻¹. HRMS (+ESI): Found m/z 692.4828, [M]⁺², C₄₁H₆₂N₄O₅ required 692.4866.

3.1.10. (S)-6-(((3R,5R,8R,9S,10S,13R,14S,17R)-17-((R)-5-(((S)-3-(1H-Indol-3-yl)-1-methoxy-1-oxopropan-2-yl)amino)-5-oxopentan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[α]phenanthren-3-yl)oxy)-6-oxohexane-1,5-diaminium chloride (16d)

The title compound was synthesised from 10d (0.527 g, 0.582 mmol) following general synthetic procedure C. The product 16d was obtained as a white solid (0.416 g, 96%); mp 205.7 °C; ¹H NMR (400 MHz, DMSO-d₆) δ 0.60 (s, 3H, CH₃), 0.86 (d, J = 6.4 Hz, 3H, CH₃), 0.91 (s, 3H, CH₃), 1.02–2.75 (m, CH, CH₂, steroid), 2.98–3.17 (m, 2H, -CO-CH-CH₂), 3.48 (s, 3H, OCH₃), 3.92 (q, J = 5.8 Hz, 1H, -CO-CH-NH₃), 4.48 (td, J = 8.1, 5.8 Hz, 1H, -CO-CH-NH), 4.76 (tt, J = 11.0, 4.6 Hz, 1H, OCH), 6.94–7.02 (m, 1H, ArH), 7.07 (ddd, J = 8.2, 6.9, 1.2 Hz, 1H, ArH), 7.14 (d, J = 2.3 Hz, 1H, ArH), 7.34 (d, J = 8.1 Hz, 1H, ArH), 7.48 (d, J = 7.9 Hz, 1H, ArH), 8.10 (d, J = 7.5 Hz, 3H, NH₃), 8.26 (d, J = 7.5 Hz, 1H, NH), 8.57–8.67 (m, 3H, NH₃), 10.93 (d, J = 2.4 Hz, 1H, NH-indole); ¹³C NMR (75 MHz, DMSO-d₆) δ 173.2, 173.1, 169.3, 136.6, 127.5, 124.1, 121.4, 118.8, 118.4, 111.9, 110.0, 76.2, 66.8, 56.4, 56.1, 53.6, 52.2, 52.1, 52.1, 42.2, 41.6, 40.0, 38.6, 35.8, 35.4, 34.8, 34.6, 32.5, 32.2, 31.8, 29.8, 28.2, 27.5, 27.5, 27.1, 26.6, 24.3, 23.4, 21.6, 20.9, 18.8, 12.3; IR (ATR): ν_max 2857, 2281, 2171, 2109, 1722, 619, 1494, 1439, 1364, 1223, 1124, 1067, 1001, 892 cm⁻¹; HRMS (+ESI): Found m/z 705.4953, [M]²⁺, C₄₂H₆₄N₄O₅ required 705.4950.

3.1.11. 2,2′-(((3R,5S,7R,8R,9S,10S,13R,14S,17R)-17-((R)-5-(((S)-3-(1H-Indol-3-yl)-1-methoxy-1-oxopropan-2-yl)amino)-5-oxopentan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[α]phenanthrene-3,7-diyl)bis(oxy))bis(2-oxoethan-1-aminium) chloride (17a)

The title compound was synthesised from 11a (0.300 g, 0.331 mmol) following general synthetic procedure C. The product 17a was obtained as a white solid (0.218 g, 85%); mp 253.1 °C; ¹H NMR (400 MHz, DMSO-d₆) δ 0.61 (s, 3H, CH₃), 0.87 (d, J = 6.4 Hz, 3H, CH₃), 0.92 (s, 3H, CH₃), 1.02–2.11 (m, CH, CH₂, steroid), 2.97–3.17 (m, 2H, -CO-CH-CH₂), 3.57 (s, 3H, OCH₃), 3.75–3.93 (m, 4H, 2 × -CO-CH₂-NH₃), 4.47 (td, J = 8.2, 5.7 Hz, 1H, -CO-CH-NH), 4.63 (dd, J = 10.5, 5.6 Hz, 1H, OCH), 4.92 (d, J = 3.6 Hz, 1H, OCH), 6.94–7.02 (m, 1H, ArH), 7.07 (ddd, J = 8.2, 7.0, 1.2 Hz, 1H, ArH), 7.14 (d, J = 2.3 Hz, 1H, ArH), 7.34 (d, J = 8.1 Hz, 1H, ArH), 7.48 (d, J = 7.8 Hz, 1H, ArH), 8.25 (d, J = 7.5 Hz, 1H, NH), 8.44 (d, J = 9.9 Hz, 6H, 3 × NH₃), 10.91 (d, J = 2.4 Hz, 1H, NH-indole); ¹³C NMR (100 MHz, DMSO-d₆) δ 173.2, 173.1, 167.7, 167.4, 136.6, 127.5, 124.1, 121.4, 118.8, 118.4, 111.9, 110.1, 75.8, 55.9, 53.6, 52.2, 50.1, 42.8, 37.5, 35.3, 34.8, 34.0, 27.5, 22.6, 18.8, 12.0; IR (ATR): ν_max 3181, 2928, 2861, 2621, 1734, 1651, 1498, 1434, 1367, 1230, 1118, 892 cm⁻¹; HRMS (+ESI): Found m/z 354.2230, [M]²⁺, C₄₀H₅₈N₄O₇ required 354.2226.

3.1.12. 4,4′-(((3R,5S,7R,8R,9S,10S,13R,14S,17R)-17-((R)-5-(((S)-3-(1H-Indol-3-yl)-1-methoxy-1-oxopropan-2-yl)amino)-5-oxopentan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[α]phenanthrene-3,7-diyl)bis(oxy))bis(4-oxobutan-1-aminium) chloride (17b)

The title compound was synthesised from 11b (0.760 g, 0.789 mmol) following general synthetic procedure C. The product 17b was obtained as a white solid (0.467 g, 71%); mp 149.0 °C; ¹H NMR (400 MHz, DMSO-d₆) δ 0.60 (s, 3H, CH₃), 0.86 (d, J = 6.3 Hz, 3H, CH₃), 0.91 (s, 3H, CH₃), 1.09–2.51 (m, CH, CH₂, steroid), 2.80 (t, J = 6.9 Hz, 4H, 2 × -CH₂-NH₃), 2.98–3.17 (m, 2H, -CO-CH-CH₂), 3.57 (s, 3H, OCH₃), 4.50 (dtd, J = 21.5, 9.1, 8.0, 4.8 Hz, 2H, -OCH, -CO-CH-NH), 4.80 (q, J = 3.0 Hz, 1H, -OCH), 6.98 (t, J = 7.4 Hz, 1H, ArH), 7.02–7.11 (m, 1H, ArH), 7.15 (d, J = 2.4 Hz, 1H, ArH), 7.34 (d, J = 8.0 Hz, 1H, ArH), 7.48 (d, J = 7.8 Hz, 1H, ArH), 8.15 (d, J = 8.9 Hz, 6H, 2 × NH₃), 8.28 (d, J = 7.5 Hz, 1H, NH), 10.93 (d, J = 2.4 Hz, 1H, NH-indole); ¹³C NMR (100 MHz, DMSO-d₆) δ 173.2, 173.1, 172.1, 172.0, 136.6, 127.5, 124.1, 121.4, 118.8, 118.4, 111.9, 110.1, 74.0, 71.4, 65.4, 55.8, 53.6, 52.2, 50.6, 42.7, 40.6, 40.4, 40.2, 40.0, 39.8, 39.6, 39.4, 38.6, 38.5, 37.6, 35.3, 34.8, 34.8, 34.2, 32.4, 31.6, 31.3, 27.5, 23.0, 22.8, 18.8, 15.6, 12.0; ν_max 3745, 2954, 2832, 2475, 2285, 2103, 2177, 2103, 2047, 1995, 1944, 1719, 1589, 1492, 1236, 1068, 1128, 991, 916 cm⁻¹; HRMS (+ESI): Found m/z 763.5000, [M]⁺, C₄₄H₆₆N₄O₇ required 763.5004.

3.1.13. (4S,4′S)-5,5′-(((3R,5S,7R,8R,9S,10S,13R,14S,17R)-17-((R)-5-(((S)-3-(1H-Indol-3-yl)-1-methoxy-1-oxopropan-2-yl)amino)-5-oxopentan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[α]phenanthrene-3,7-diyl)bis(oxy))bis(5-oxopentane-1,4-diaminium) chloride (17c)

The title compound was synthesised from 11c (0.511 g, 0.418 mmol) following general synthetic procedure C. The product 17c was obtained as a white solid (0.305 g, 75%); mp 235.9 °C; ¹H NMR (400 MHz, DMSO-d₆) δ 0.60 (s, 3H, CH₃), 0.87 (d, J = 6.2 Hz, 3H, CH₃), 0.92 (d, J = 3.9 Hz, 3H, CH₃), 1.23– 2.18 (m, CH, CH₂, steroid), 2.83 (m, 4H, 2 × -CH₂-NH₃), 2.97–3.19 (m, 2H, CH₂-CH-NH), 3.57 (d, J = 2.8 Hz, 3H, OCH₃), 3.96–4.13 (m, 2H, 2 × CH-NH₃), 4.45 (ddd, J = 11.8, 8.1, 6.1 Hz, 1H, CO-CH-NH), 4.55–4.70 (m, 1H, OCH), 4.87 (q, J = 2.9 Hz, 1H, OCH), 6.95–7.01 (m, 1H, ArH), 7.03–7.10 (m, 1H, ArH), 7.15 (dd, J = 5.0, 2.4 Hz, 1H, ArH), 7.34 (d, J = 8.1 Hz, 1H, ArH), 7.48 (q, J = 8.6, 7.6 Hz, 1H, ArH), 8.27–8.34 (m, 6H, 2 × NH₃), 8.70–8.92 (m, 7H, 2 × NH₃, NH), 10.97 (d, J = 2.4 Hz, 1H, NH-indole); ¹³C NMR (100 MHz, DMSO-d₆) δ 173.2, 173.1, 169.3, 136.6, 129.5, 127.5, 124.2, 121.4, 121.0, 118.8, 118.7, 112.0, 110.0, 76.5, 76.2, 74.3, 73.8, 65.4, 55.9, 55.8, 53.6, 52.6, 52.2, 52.0, 51.9, 51.8, 49.9, 42.9, 42.8, 38.7, 37.7, 34.9, 34.8, 29.8, 23.7, 23.2, 23.1, 22.6, 22.5, 20.8, 18.8, 15.6, 12.0, 12.0; IR (ATR): ν_max 3745, 2868, 2653, 2284, 2171, 2041, 1730, 1596, 1496, 1456, 1365, 1223, 1139, 1048, 1001, 890 cm⁻¹; HRMS (+ESI): Found m/z 411.2809, [M]⁴⁺, C₄₆H₇₄N₆O₇ required 411.2804.

3.1.14. (5S,5′S)-6,6′-(((3R,5S,7R,8R,9S,10S,13R,14S,17R)-17-((R)-5-(((S)-3-(1H-Indol-3-yl)-1-methoxy-1-oxopropan-2-yl)amino)-5-oxopentan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[α]phenanthrene-3,7-diyl)bis(oxy))bis(6-oxohexane-1,5-diaminium) chloride (17d)

The title compound was synthesised from 11d (0.061 g, 0.048 mmol) following general synthetic procedure C. The product 17d was obtained as a white solid (0.041 g, 85%); mp 244.2 °C; ¹H NMR (600 MHz, DMSO-d₆) δ 0.60 (s, J = 5.0 Hz, 3H, CH₃), 0.87 (d, J = 6.1 Hz, 3H, CH₃), 0.92 (s, 3H, CH₃), 0.98–2.07 (m, steroid), 2.75–2.81 (m, 4H, 2 × -CH₂-NH₃), 3.03–3.15 (m, 2H, -CO-CH-CH₂), 3.57 (s, 3H, OCH₃), 3.90 (d, J = 5.7 Hz, 1H, -CH-NH₃), 4.05 (t, J = 5.8 Hz, 1H, -CH-NH₃), 4.46 (td, J = 8.0, 5.8 Hz, 1H, -CO-CH-NH), 4.60 (dt, J = 11.3, 6.4 Hz, 1H, OCH), 4.89 (t, J = 3.3 Hz, 1H, OCH), 6.98 (t, J = 7.5 Hz, 1H, ArH), 7.07 (t, J = 7.5 Hz, 1H, ArH), 7.15 (dd, J = 7.7, 2.4 Hz, 1H, ArH), 7.35 (d, J = 8.1 Hz, 1H, ArH), 7.48 (d, J = 8.0 Hz, 1H, ArH), 8.07–8.19 (m, 6H, 2 × NH₃), 8.30 (dd, J = 28.8, 7.5 Hz, 1H, NH), 8.63–8.73 (m, 6H, 2 × NH₃), 10.93 (d, J = 2.5 Hz, 1H, NH); ¹³C NMR (150 MHz, DMSO-d₆) δ 173.4, 173.1, 169.2, 136.6, 127.5, 124.2, 124.1, 121.4, 118.9, 118.4, 112.0, 110.0, 76.1, 74.1, 72.7, 72.6, 71.0, 66.8, 65.4, 60.7, 60.6, 53.7, 52.6, 52.3, 52.2, 50.4, 44.1, 42.8, 42.7, 38.7, 38.6, 35.4, 34.8, 34.6, 34.5, 33.9, 32.6, 29.8, 28.0, 27.5, 26.7, 26.7, 23.7, 22.7, 22.5, 18.8, 15.6, 12.0; IR (ATR): ν_max 3365, 3236, 2930, 2865, 2072, 1733, 1638, 1510, 1457, 1218, 1137, 1065, 999 cm⁻¹; HRMS (+ESI): Found m/z 425.2962, [M]⁴⁺, C₄₈H₇₂N₆O₇ required 425.2961.

3.1.15. 2,2′-(((3R,5R,8R,9S,10S,12S,13R,14S,17R)-17-((R)-5-(((S)-3-(1H-Indol-3-yl)-1-methoxy-1-oxopropan-2-yl)amino)-5-oxopentan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[α]phenanthrene-3,12-diyl)bis(oxy))bis(2-oxoethan-1-aminium) chloride (18a)

The title compound was synthesised from 12a (0.173 g, 0.191 mmol) following general synthetic procedure C. The product 18a was obtained as a white solid (0.130 g, 87%); mp 242.9 °C; ¹H NMR (300 MHz, DMSO-d₆) δ 0.69 (s, 3H, CH₃), 0.75 (d, J = 6.2 Hz, 3H, CH₃), 0.90 (s, 3H, CH₃), 1.01– 2.19 (m, CH, CH_2, steroid), 2.98–3.20 (m, 2H, -CO-CH-CH₂), 3.57 (s, 3H, OCH₃), 3.74 (d, J = 5.2 Hz, 2H, -CO-CH₂-NH₃), 3.83–3.95 (m, 2H, -CO-CH₂-NH₃), 4.47 (td, J = 8.1, 5.8 Hz, 1H, -CO-CH-NH), 4.72 (tt, J = 10.8, 5.1 Hz, 1H, OCH), 5.13 (d, J = 2.8 Hz, 1H, OCH), 6.98 (td, J = 7.4, 7.0, 1.1 Hz, 1H, ArH), 7.07 (ddd, J = 8.1, 7.0, 1.2 Hz, 1H, ArH), 7.16 (d, J = 2.4 Hz, 1H, ArH), 7.34 (d, J = 8.0 Hz, 1H, ArH), 7.48 (d, J = 7.8 Hz, 1H, ArH), 8.28 (d, J = 7.5 Hz, 1H, ArH), 8.47–8.63 (m, 6H, 2 × NH₃), 10.97 (d, J = 2.4 Hz, 1H, NH-indole); ¹³C NMR (75 MHz, DMSO-d₆) δ 173.3, 173.1, 167.5, 167.4, 136.6, 127.5, 124.1, 121.4, 118.8, 118.4, 111.9, 110.0, 77.6, 76.2, 65.4, 53.6, 52.2, 49.0, 47.4, 45.1, 41.5, 40.0, 40.0, 35.6, 34.9, 34.6, 34.2, 32.4, 32.1, 31.5, 27.5, 27.0, 26.5, 26.0, 25.7, 23.5, 23.1, 17.8, 15.6, 12.4; IR (ATR): ν_max 3237, 2923, 2863, 2321, 1920, 1735, 1640, 1509, 1435, 1367, 1236, 1120, 1049, 967, 913 cm⁻¹; HRMS (+ESI): Found m/z 354.2226, [M]²⁺, C₄₀H₅₈N₄O₇ required 354.2226.

3.1.16. 2,2′-(((3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-17-((R)-5-(((S)-3-(1H-Indol-3-yl)-1-methoxy-1-oxopropan-2-yl)amino)-5-oxopentan-2-yl)-3-(2-aminoacetoxy)-10,13-dimethylhexadecahydro-1H-cyclopenta[α]phenanthrene-7,12-diyl)bis(oxy))bis(2-oxoethan-1-aminium) chloride (19a)

The title compound was synthesised from 13a (0.500 g, 0.559 mmol) following general synthetic procedure C. The product 19a was obtained as a white solid (0.426 g, 87%); mp 221.4 °C; ¹H NMR (400 MHz, DMSO-d₆) δ 0.69 (s, 3H), 0.76 (d, J = 6.3 Hz, 3H), 0.92 (s, 3H), 1.03–3.17 (m, CH, CH₂, steroid), 3.57 (s, 3H, OCH₃), 3.70–4.03 (m, 6H, 3 × -CO-CH₂-NH₃), 4.46 (td, J = 8.0, 5.6 Hz, 1H, -CO-CH-NH), 4.59 (tq, J = 9.0, 4.3 Hz, 1H, OCH), 4.89–4.96 (m, 1H, OCH), 5.13 (t, J = 2.9 Hz, 1H, OCH), 6.98 (t, J = 7.4 Hz, 1H, ArH), 7.07 (t, J = 7.5 Hz, 1H, ArH), 7.16 (d, J = 2.4 Hz, 1H, ArH), 7.35 (d, J = 8.1 Hz, 1H, ArH), 7.48 (d, J = 7.9 Hz, 1H, ArH), 8.27 (d, J = 7.4 Hz, 1H, NH), 8.44–8.70 (m, J = 7.5, 7.1 Hz, 9H, 3 × NH₃), 10.97 (d, J = 2.4 Hz, 1H, NH-indole); ¹³C NMR (100 MHz, DMSO-d₆) δ 124.1, 121.4, 118.9, 118.4, 111.9, 77.3, 76.0, 73.2, 72.7, 72.7, 69.7, 66.8, 66.8, 60.7, 52.2, 47.4, 43.0, 40.6, 40.4, 40.3, 40.2, 40.0, 39.8, 37.2, 34.8, 28.6, 27.5, 22.5, 17.7, 12.2; ν_max 3194, 2936, 2859, 2616, 2342, 1734, 1640, 1597, 1508, 1435, 1368, 1232, 1117, 1039, 742 cm⁻¹; HRMS (+ESI): Found m/z 390.7312, [M]³⁺, C₄₂H₆₃N₅O₉ required 390.7307.

3.1.17. 4,4′,4″-(((3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-17-((R)-5-(((S)-3-(1H-Indol-3-yl)-1-methoxy-1-oxopropan-2-yl)amino)-5-oxopentan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[α]phenanthrene-3,7,12-triyl)tris(oxy))tris(4-oxobutan-1-aminium) chloride (19b)

The title compound was synthesised from 13b (0.138 g, 0.551 mmol) following general synthetic procedure C. The product 19b was obtained as a white solid (0.094 g, 81%); mp 212.2 °C; ¹H NMR (400 MHz, DMSO-d₆) δ 0.64–0.69 (m, 3H, CH₃), 0.73 (d, J = 6.2 Hz, 3H, CH₃), 0.88 (d, J = 15.8 Hz, 3H, CH₃), 1.01– 2.64 (m, CH, CH₂, steroid), 2.78–2.91 (m, 6H, 3 × CH₂-NH₃), 3.02–3.15 (m, 2H, CO-CH-CH₂), 3.57 (s, 3H, OCH₃), 4.48 (dtd, J = 15.8, 9.0, 8.1, 4.7 Hz, 2H, OCH, CO-CH-NH), 4.76–4.86 (m, 1H, OCH), 4.99 (dt, J = 7.0, 2.9 Hz, 1H, OCH), 6.98 (t, J = 7.4 Hz, 1H, ArH), 7.06 (t, J = 7.5 Hz, 1H, ArH), 7.17 (q, J = 2.6 Hz, 1H, ArH), 7.34 (d, J = 8.0 Hz, 1H, ArH), 7.50 (dd, J = 17.0, 7.8 Hz, 1H, NH), 8.12–8.31 (m, 9H, NH₃), 10.90–10.98 (m, 1H, NH-indole); ¹³C NMR (100 MHz, DMSO-d₆) δ 124.2, 121.4, 118.8, 118.4, 111.9, 75.2, 73.8, 71.0, 66.8, 66.8, 53.6, 52.2, 47.3, 43.5, 40.6, 40.5, 40.3, 40.0, 38.7, 38.6, 37.3, 34.8, 34.5, 32.2, 31.7, 31.6, 31.4, 31.2, 31.0, 28.7, 27.5, 27.1, 26.9, 25.5, 23.2, 23.0, 22.9, 22.8, 22.6, 17.9, 12.3; ν_max 2932, 2071, 1934, 1719, 1639, 1510, 1438, 1379, 1191, 1113, 1057, 1005, 749 cm⁻¹; HRMS (+ESI): Found m/z 288.8543, [M]³⁺, C₄₈H₇₆N₅O₉ required 288.8542.

3.1.18. (4S,4′S,4″S)-5,5′,5″-(((3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-17-((R)-5-(((S)-3-(1H-Indol-3-yl)-1-methoxy-1-oxopropan-2-yl)amino)-5-oxopentan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[α]phenanthrene-3,7,12-triyl)tris(oxy))tris(5-oxopentane-1,4-diaminium) chloride (19c)

The title compound was synthesised from 13c (0.200 g, 0.129 mmol) following general synthetic procedure C. The product 19c was obtained as a white solid (0.113 g, 75%); mp 241.6 °C; ¹H NMR (600 MHz, DMSO-d₆) δ 0.70 (d, J = 1.9 Hz, 3H, -CH₃), 0.75 (dd, J = 16.3, 6.6 Hz, 3H, CH₃), 0.93 (d, J = 7.8 Hz, 3H, CH₃), 1.02–2.41 (m, CH, CH₂, steroid), 2.88 (d, J = 32.7 Hz, 4H, 2 × -CH₂-NH₃), 3.00–3.18 (m, 4H, -CH₂-NH_3, -CO-CH-CH₂), 3.57 (d, J = 1.5 Hz, 3H, -OCH₃), 4.15 (d, J = 5.5 Hz, 1H, -CO-CH-NH₃), 4.32 (s, 1H, -CO-CH-NH₃), 4.45 (m, J = 7.5, 5.9, 3.5 Hz, 2H, -OCH, -CO-CH-NH₃), 4.59 (t, J = 5.6 Hz, 1H, -CO-CH-NH), 5.05 (dq, J = 20.3, 3.0 Hz, 1H, OCH), 5.15 (q, J = 2.9 Hz, 1H, OCH), 6.98 (ddd, J = 8.0, 7.0, 1.1 Hz, 1H, ArH), 7.07 (ddd, J = 8.1, 6.9, 1.2 Hz, 1H, ArH), 7.17 (q, J = 4.8 Hz, 1H, ArH), 7.34–7.36 (m, 1H, ArH), 7.48 (d, J = 7.9 Hz, 1H, ArH), 8.27–8.41 (m, 10H, -NH, 3 × NH₃), 8.69–8.87 (m, 9H, 3 × NH₃), 10.93–10.99 (m, 1H, NH-indole); ¹³C NMR (150 MHz, DMSO-d₆) δ 173.3, 173.1, 169.3, 169.2, 169.1, 167.7, 136.6, 127.5, 124.2, 121.4, 118.8, 118.4, 112.0, 110.0, 77.6, 76.7, 73.0, 53.6, 52.2, 51.9, 51.9, 51.8, 49.3, 47.4, 45.3, 45.3, 45.2, 42.6, 41.3, 38.8, 38.6, 38.4, 37.6, 35.0, 34.7, 32.6, 31.6, 31.5, 28.4, 27.8, 27.4, 27.1, 23.5, 22.9, 22.8, 22.4, 22.2, 20.7, 17.7, 12.3, 12.2; ν_max 3373, 2863, 2079, 1948, 1731, 1599, 1509, 1457, 1365, 1226, 1128, 1004, 894 cm⁻¹; HRMS (+ESI): Found m/z 317.8809, [M]⁶⁺, C₅₁H₈₅N₈O₉ required 317.8808.

3.1.19. (5S,5′S,5″S)-6,6′,6″-(((3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-17-((R)-5-(((S)-3-(1H-Indol-3-yl)-1-methoxy-1-oxopropan-2-yl)amino)-5-oxopentan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[α]phenanthrene-3,7,12-triyl)tris(oxy))tris(6-oxohexane-1,5-diaminium) chloride (19d)

The title compound was synthesised from 13d (0.282 g, 0.177 mmol) following general synthetic procedure C. The product 19d was obtained as a white solid (0.185 g, 86%); mp 243.3 °C; ¹H NMR (600 MHz, DMSO-d₆) δ 0.70 (d, J = 3.0 Hz, 3H, CH₃), 0.73–0.79 (m, 3H, CH₃), 0.92 (dd, J = 6.6, 2.6 Hz, 3H, CH₃), 1.05–2.26 (m, CH, CH₂, steroid), 2.77–2.84 (m, 6H, 3 × -CH₂-NH₃), 3.01–3.15 (m, 2H, -CO-CH-CH₂), 3.38 (d, J = 7.0 Hz, 3H, 3 × -CO-CH-NH₃), 3.57 (s, 3H, OCH₃), 3.74–5.19 (m, 4H, -OCH, -CO-CH-NH), 6.95–7.01 (m, 1H, ArH), 7.06 (ddd, J = 8.1, 6.9, 1.1 Hz, 1H, ArH), 7.17 (q, J = 3.5, 2.9 Hz, 1H, ArH), 7.35 (d, J = 8.1 Hz, 1H, ArH), 7.47 (d, J = 7.9 Hz, 1H, ArH), 8.04–8.14 (m, 3H, NH₃), 8.19–8.25 (m, 6H, 2 × NH₃), 8.33 (dt, J = 7.9, 3.2 Hz, 1H, NH), 8.65–8.90 (m, 9H, 3 × NH₃), 10.97 (d, J = 2.2 Hz, 1H, NH-indole). ¹³C NMR (150 MHz, DMSO-d₆) δ 173.4, 173.3, 173.1, 172.5, 169.3, 136.6, 127.5, 124.2, 121.4, 118.8, 118.4, 112.0, 110.0, 77.6, 76.7, 73.0, 65,4, 53.7, 52.2, 45.3, 45.3, 45.2, 42.8, 38.8, 38.8, 38.6, 38.4, 37.5, 35.0, 34.8, 32.6, 34.5, 34.3, 32.7, 31.7, 31.5, 31.1, 30.8, 29.8, 29.8, 29.3, 28.3, 27.6, 27.4, 26.7, 26.5, 25.6, 22.4, 22.4, 21.7, 17.7, 15.6, 12.3, 12.2; ν_max 3377, 2920, 2866, 2045, 1732, 1602, 1509, 1456, 1364, 1224, 1129, 1068, 994, 893 cm⁻¹; HRMS (+ESI): Found m/z 331.8966, [M]⁶⁺, C₅₄H₉₁N₈O₉ required 331.8964.

3.1.20. (4S,4′S)-5,5′-(((3R,5S,7R,8R,9S,10S,13R,14S,17R)-17-((R)-5-(((S)-1-Methoxy-1-oxo-3-phenylpropan-2-yl)amino)-5-oxopentan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[α]phenanthrene-3,7-diyl)bis(oxy))bis(5-oxopentane-1,4-diaminium) chloride (20)

The title compound was synthesised from 14 (0.783 g, 0.662 mmol) following general procedure C. The product 20 was obtained as a white solid (0.532 g, 87%); mp 236.6 °C; ¹H NMR (400 MHz, DMSO-d₆) δ 0.60 (s, 3H, CH₃), 0.85 (d, J = 6.3 Hz, 3H, CH₃), 0.92 (d, J = 4.2 Hz, 3H, CH₃), 1.05–2.92 (m, CH, CH₂, steroid), 2.96–3.06 (m, 2H, -CO-CH-CH₂), 3.58 (d, J = 1.0 Hz, 3H, OCH₃), 3.89–4.19 (m, 2H, CH, CH-NH₃), 4.36–4.70 (m, 3H, 2 × OCH, -CO-CH-CH₂), 4.87 (d, J = 3.2 Hz, 1H, CH), 7.21 (d, J = 2.0 Hz, 1H, ArH), 7.23 (d, J = 2.0 Hz, 2H, ArH), 7.26 (s, 1H, NH), 7.27 (d, J = 1.4 Hz, 1H, ArH), 7.30 (t, J = 3.2 Hz, 1H, ArH), 8.26–8.38 (m, 6H, NH₃), 8.81 (d, J = 35.9 Hz, 6H, NH₃); ¹³C NMR (100 MHz, DMSO-d₆) δ 173.2, 173.2, 172.7, 171.8, 169.4, 169.3, 169.1, 168.8, 137.9, 137.4, 129.6, 129.5, 128.8, 128.7, 127.2, 127.0, 76.5, 55.9, 54.7, 54.1, 52.6, 52.2, 52.0, 51.2, 49.8, 42.8, 42.8, 38.7, 38.5, 38.1, 37.7, 37.1, 36.8, 35.4, 34.9, 34.8, 34.4, 33.4, 23.2, 22.6, 22.5, 18.8, 12.0, 12.0; IR (ATR): ν_max 3374, 2929, 2865, 2061, 1733, 1639, 1510, 1449, 1366, 1223, 1138, 1065, 997; HRMS (+ESI): Found m/z 391.7755, [M]⁴⁺, C₄₄H₇₃N₅O₇ required 391.7745.

3.1.21. (4S,4′S)-5,5′-(((3R,5S,7R,8R,9S,10S,13R,14S,17R)-17-((R)-5-(Benzylamino)-5-oxopentan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthrene-3,7-diyl)bis(oxy))bis(5-oxopentane-1,4-diaminium) chloride (21)

The title compound was synthesised from 15 (0.520 g, 0.468 mmol) following general procedure C. The product 21 was obtained as a white solid (0.374 g, 93%); mp 208.3 °C; ¹H NMR (400 MHz, DMSO-d₆) δ 0.61 (s, 3H, CH₃), 0.88–0.94 (m, 6H, 2 × CH₃), 1.02–2.23 (m, CH, CH₂, steroid), 2.82 (dq, J = 12.8, 6.4 Hz, 4H, 2 × CH_2-NH₃), 3.95–4.31 (m, 5H, OCH, 2 × CH-NH₃, NH-CH₂), 4.53–4.71 (m, 1H, OCH), 7.21–7.32 (m, 5H, ArH), 8.21–8.34 (m, 6H, NH₃), 8.42 (dt, J = 14.5, 6.0 Hz, 1H, NH), 8.78 (dd, J = 26.3, 12.0 Hz, 6H, NH₃); IR (ATR): ν_max 3369, 2927, 2862, 1942, 1733, 1509, 1601, 1509, 1450, 1378, 1226, 1138, 1065, 962, 916; HRMS (+ESI): Found m/z 355.7646, [M]⁴⁺, C₄₁H₆₉N₅O₅ required 355.7644.

3.1.22. ((4-(((3R,5R,8R,9S,10S,13R,14S,17R)-17-((R)-5-(((S)-3-(1H-Indol-3-yl)-1-methoxy-1-oxopropan-2-yl)amino)-5-oxopentan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[α]phenanthren-3-yl)oxy)-4-oxobutyl)amino)(amino)methaniminium trifluoroacetate (23)

The title compound was synthesised from 16b (0.213 g, 0.305 mmol), N,N′-di-Boc-1H-pyrazole-1-carboxamidine (0.125 g, 0.396 mmol) and triethylamine (1 mL, 0.762 mmol) following general synthetic procedure D. The product 23 was obtained as a white solid (0.105 g, 53%); mp 247.9 °C; ¹H NMR (400 MHz, DMSO-d₆) δ 0.60 (s, 3H, CH₃), 0.86 (d, J = 6.3 Hz, 3H, CH₃), 0.91 (s, 3H, CH₃), 1.04–2.55 (m, CH, CH_2, steroid), 3.02–3.12 (m, 2H, -CO-CH-CH₂), 3.57 (s, 3H, OCH₃), 4.48 (q, J = 7.2 Hz, 1H, -CO-CH-NH), 4.56–4.73 (m, 1H, OCH), 6.81–7.72 (m, 10H, ArH, NH₂, NH), 8.22 (d, J = 7.6 Hz, 1H, NH), 10.87 (s, 1H, NH-indole); ¹³C NMR (75 MHz, DMSO-d₆) δ 173.2, 173.1, 172.3, 170.9, 157.3, 136.6, 127.6, 124.1, 121.4, 118.8, 118.5, 111.9, 110.1, 56.4, 56.1, 53.6, 52.2, 42.7, 41.7, 40.8, 40.5, 40.3, 40.0, 39.7, 39.1, 35.8, 35.4, 35.0, 34.7, 33.9, 32.5, 31.3, 27.5, 27.1, 26.4, 24.5, 24.3, 23.5, 20.9, 18.8, 12.3; IR (ATR): ν_max 3275, 3184, 2930, 2863, 2320, 1654, 1533, 1439, 1357, 1175, 1132, 800 cm⁻¹; HRMS (+ESI): Found m/z 704.4746, [M]⁺, C₄₁H₆₁N₅O₅ required 704.4746.

3.1.23. (S)-6-((2-(((3R,5R,8R,9S,10S,13R,14S,17R)-17-((R)-5-(((S)-3-(1H-Indol-3-yl)-1-methoxy-1-oxopropan-2-yl)amino)-5-oxopentan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[α]phenanthren-3-yl)oxy)-2-oxoethyl)amino)-6-oxohexane-1,5-diaminium chloride (25)

The title compound was synthesised from 16a (0.150 g, 0.224 mmol), N_ε-Z-N_α-Boc-L-lysine hydroxysuccinimide ester (0.119 g, 0.269 mmol) and DIPEA (0.116 g, 0.895 mmol) following general synthetic procedure E. The product 25 was obtained as a white solid (0.027 g, 15%); mp 247.9 °C; ¹H NMR (400 MHz, DMSO-d₆) δ 0.60 (s, 3H, CH₃), 0.86 (d, J = 6.4 Hz, 3H, CH₃), 0.91 (s, 3H, CH₃), 1.01–2.15 (m, steroid), 2.75 (q, J = 8.3, 7.2 Hz, 2H, -CH2-NH3), 3.02–3.13 (m, 2H, -CO-CH-CH₂), 3.57 (s, 3H, -OCH₃), 3.82–3.90 (m, 2H, -CH_2-NH), 3.96 (dd, J = 17.5, 5.9 Hz, 1H, -CH-NH₃), 4.48 (td, J = 8.1, 5.8 Hz, 1H, -CO-CH-NH), 4.62–4.72 (m, 1H, -OCH), 6.98 (t, J = 7.4 Hz, 1H, ArH), 7.07 (t, J = 7.5 Hz, 1H, ArH), 7.14 (d, J = 2.4 Hz, 1H, ArH), 7.34 (d, J = 8.1 Hz, 1H, ArH), 7.49 (d, J = 7.9 Hz, 1H, ArH), 8.05 (s, 3H, NH₃), 8.25 (d, J = 7.6 Hz, 1H, NH), 8.35 (d, J = 5.3 Hz, 3H, NH₃), 9.05 (t, J = 5.8 Hz, 1H, NH), 10.90 (d, J = 2.3 Hz, 1H, NH-indole); ¹³C NMR (75 MHz, DMSO-d₆) δ 173.2, 173.1, 169.6, 169.3, 136.6, 127.5, 124.1, 121.4, 118.8, 118.5, 111.9, 110.1, 75.1, 66.8, 66.8, 65.4, 56.4, 56.1, 53.6, 52.2, 52.1, 42.7, 41.6, 40.8, 40.5, 40.3, 40.0, 39.7, 39.4, 39.1, 38.7, 35.8, 35.4, 34.9, 34.6, 30.8, 27.5, 26.7, 23.5, 21.3, 20.9, 18.7, 15.6, 12.3; IR (ATR): ν_max 3229, 3016, 2926, 2863, 1733, 1651, 1509, 1439, 1207, 1097, 1009, 976 cm⁻¹; HRMS (+ESI): Found m/z 762.5160, [M]²⁺, C₄₄H₆₇N₅O₆ required 762.5164.

3.1.24. 1,1′,1″-(((((3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-17-((R)-5-(((S)-3-(1H-Indol-3-yl)-1-methoxy-1-oxopropan-2-yl)amino)-5-oxopentan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[α]phenanthrene-3,7,12-triyl)tris(oxy))tris(2-oxoethane-2,1-diyl))tris(azanediyl))tris(3-methyl-1-oxobutan-2-aminium) chloride (27)

The title compound was synthesised from 19a (0.250 g, 0.281 mmol), Boc-Val-OH (0.371 g, 1.687 mmol), EDC.HCl (0.323 g, 1.687 mmol), 1-hydroxybenzotriazole (HOBt) hydrate (0.153 g, 0.843 mmol) and DIPEA (0.25 mL, 1.41 mmol) following general procedure F. The product 27 was obtained as a white solid (0.029 g, 10%); mp 242.9 °C; ¹H NMR (600 MHz, DMSO-d₆) δ 0.69 (s, 3H, CH₃), 0.72–0.76 (d, 3H, CH₃), 0.91 (s, 3H, CH₃), 1.00 (d, J = 8.2 Hz, 18H, 3 × CH₃-valine side chain), 1.04–3.57 (m, CH, CH_2, steroid), 3.12 (d, 2H, -CO-CH-CH₂), 3.57 (s, 3H, OCH₃), 3.69 (s, 3H, 3 × -CO-CH-NH₃), 3.83 (s, 2H, -NH-CH₂-CO), 3.99–4.07 (m, 4H, 2 × -NH-CH₂-CO), 4.45–4.49 (m, 1H, -CO-CH-NH), 4.54 (s, 1H, -OCH), 4.85 (s, 1H, -OCH), 5.07 (s, 1H, -OCH), 6.96–7.01 (m, 1H, ArH), 7.04–7.10 (m, 1H, ArH), 7.15 (s, 1H, ArH), 7.35 (d, J = 8.0 Hz, 1H, ArH), 7.48 (d, J = 7.9 Hz, 1H, ArH), 8.24–8.33 (m, 10H, 3 × NH₃, NH), 9.01 (s, 1H, NH), 9.03–9.07 (m, 1H, NH), 9.10–9.14 (m, 1H, NH), 10.93 (s, 1H, NH-indole); ¹³C NMR (150 MHz, DMSO-d₆) δ 173.2, 173.1, 169.2, 169.2, 168.9, 168.8, 136.6, 127.5, 124.1, 121.4, 118.9, 118.4, 112.0, 110.0, 76.1, 74.9, 72.0, 65.4, 57.7, 57.6, 53.7, 52.2, 47.5, 45.2, 44.8, 43.6, 41.4, 41.2, 37.3, 34.8, 34.7, 34.6, 34.5, 34.4, 32.6, 31.6, 31.2, 30.3, 30.3, 30.2, 29.8, 28.9, 28.1, 27.5, 27.4, 26.8, 25.8, 22.7, 22.6, 22.5, 18.6, 18.4, 18.2, 17.9, 15.7, 12.5, 12.4; IR (ATR): ν_max 3223, 3052, 2936, 2343, 2095, 1730, 1674, 1509, 1378, 1196, 1097, 1004 cm⁻¹; HRMS (+ESI): Found m/z 359.8915, [M]³⁺, C₅₇H₈₈N₈O₁₂ required 359.8913.

3.2. Minimal Inhibitory Concentration (MIC) Assay

The antimicrobial activities of the synthesised compounds were investigated by determining their minimum inhibitory concentration (MIC) according to a previously published protocol [22]. The bacterial strains used were S. aureus 38 (isolated from a human corneal ulcer), S. aureus 111 (isolated from a corneal ulcer; resistant to oxacillin, ciprofloxacin, ceftazidime, chloramphenicol, azithromycin and polymyxin B) [23], S. aureus 112 (isolated from a corneal ulcer; resistant to oxacillin, ciprofloxacin, ceftazidime, chloram-phenicol, azithromycin and polymyxin B) [23], E. coli K12 (ATCC 10798; isolated from faeces) and P. aeruginosa PAO1 (isolated from a wound). A single colony of bacteria was cultured overnight in trypticase soy broth (TSB) at 37 °C. Following incubation, the resulting bacterial culture was collected by centrifugation and re-suspended in TSB twice. The optical density (OD) of the resulting culture was adjusted to OD₆₆₀ = 0.1 in TSB (equivalent to 10⁸ colony forming units (CFU)/mL bacteria) and was further diluted to 10⁵ CFU/mL in TSB. Then, 100 μL of the bacterial solution was added to wells of a 96-well plate containing 100 μL serially diluted compound, with final concentration ranging from 1 to 250 μM. The plates were then incubated at 37 °C for 24 h and the data were recorded by measuring the OD value at 660 nm using a FLUOstar Omega microplate reader (BMG Labtech). The MIC value of each compound was determined as the lowest concentration that completely inhibited the growth of bacteria. Each experiment was performed in triplicate and was repeated in two independent experiments.

3.3. Tethered Bilayer Lipid Membrane Assay

Tethered bilayer lipid membranes (tBLMs), in association with AC electrical impedance spectroscopy techniques, were employed to measure changes in lipid bilayer conductance and capacitance in the presence of the test compound [30,31]. Using a previously described solvent exchange technique, sparsely tethered tBLMs were prepared using a zwitterionic 1-palmitoyl- 2-oleoyl-glycero-3-phosphocholine (POPC) or a mixture of 30% palmitoyl-oleoyl-phosphatidylglycerol (POPG) lipids with 70% POPC in order to create a negatively charged tBLM. Briefly, pre-prepared tethered benzyl-disulfide (tetra-ethyleneglycol) n = 2 C20-phytanyl tethers: benzyl-disulfide-tetra-ethyleneglycol-OH spacers in the ratio of 1:10 were coated onto a gold patterned polycarbonate slide (SDx Tethered Membranes Pty Ltd., Sydney, Australia). Using a specialised cartridge chamber, a 3-mM solution of a standard mobile lipid phase POPC (Avanti Lipids, Alabaster, AL, USA) was added to the tethering chemistries. All lipids were dissolved in 100% ethanol. To create negatively charged membranes, similar to those present in bacterial species, the same mobile lipid phase was instead supplemented with 30% POPG (Avanti Lipids, Alabaster, AL, USA). Lipids were left for 2 min to associate with the tethering chemistries before being washed with 3 × 400 mL of phosphate buffered saline (PBS). AC electrical impedance spectrometry (EIS) was used monitor any changes in membrane conduction and capacitance as a result of adding the tested compound in increasing concentrations. EIS measures employed a 50-mV peak-to-peak AC excitation from 0.1 to 2000 Hz with four steps per decade and were recorded using a TethaPodTM operated with TethaQuickTM software (SDx Tethered Membranes Pty Ltd., Sydney, Australia). The data were fitted to an equivalent circuit using a proprietary adaptation of a Lev Mar fitting routine. This circuit consists of a Constant Phase Element (CPE), to represent the imperfect capacitance of the gold tethering electrode, in series with a Resistor/Capacitor network to represent the lipid bilayer membrane [30].

3.4. Cytoplasmic Membrane Depolarisation

The method was adopted from Wu et al. [34] with slight modification. Bacterial cytoplasmic membrane permeability was determined using membrane potential sensitive dye diSC3-5 (3,3′-dipropylthiadicarbocyanine iodide), which penetrates inside bacterial cells depending on the membrane potential gradient of the cytoplasmic membrane. Bacteria were grown in Mueller Hinton Broth (MHB) to mid-log phase by incubating with shaking at 37 °C for 18–24 h. Following incubation, bacteria were washed with 5 mM HEPES containing 20 mM glucose pH 7.2 and resuspended in the same buffer to an OD₆₀₀ of 0.05–0.06, which gave 1 × 10⁷ CFU/mL. The dye diSC3-5 was added at 4 μM to the bacterial suspension. The suspensions were incubated at room temperature for 1 h in darkness for maximum dye take-up by the bacterial cells. Then, 100 mM KCl was added to balance the K+ outside and inside the bacterial cell to prevent further uptake or outflow of the dye. Next, 100 μL of bacterial suspension was added into a 96-well microtiter plate with equal volume of antimicrobial compounds. DMSO (20%) was set as a positive control while dye and only bacterial cells were set as negative control. Fluorescence was measured with a luminescence spectrophotometer at 3 min intervals at an excitation wavelength of 621 m and an emission wavelength of 670 nm.

3.5. Cell Viability Count

The number of viable cells was confirmed by serially diluting aliquots of bacteria in D/E neutralizing broth (Remel, Lenexa, KS, USA) and plating onto Tryptic Soy Agar (Oxoid, Basingstoke, UK) containing phosphatidylcholine (0.7 g/L) and Tween 80 (5 mL/L). The plates were incubated at 37 °C overnight and numbers of live bacteria were enumerated and expressed as CFU/mL. The experiment was performed in triplicate.

3.6. Toxicity Assay

The cell viability of measurement of the compounds was performed using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma Aldrich, St Louis, MO, USA) colorimetric assay with normal human lung fibroblasts MRC-5 cell. The cells were cultured in minimal essential medium (MEM, Invitrogen) and 10% FBS (Foetal Bovine Serum). The cell line was maintained at 37 °C in 5% CO₂ as an adherent monolayer and was passaged upon reaching confluence by standard cell culture techniques. MRC-5 cells were seeded at 5000 cells/well in 96-well plates to ensure full confluence (quiescence) and left overnight to adhere to the plate wells. The adherent cells were treated with 1–500 μM of compounds by dissolving in DMSO and serially diluting with media. The final concentration of DMSO was 0.5% (v/v). After 72 h drug incubation, 20 mL stock MTT solution (5 mg/mL) was added and the cells were incubated for another 3.5–4 h. The MTT solution containing media was carefully aspirated without displacing the purple crystals from the bottom, and 80 mL acid-isopropanol was added to all wells and mixed slowly by shaking with orbital shaker to dissolve the dark blue crystals. The metabolic activity was detected by spectrophotometric analysis by assessing the read absorbance (590/620 nm) on EnSight plate reader (PerkinElmer, Waltham, MA, USA), and cell viability was expressed as a percentage of untreated control cells. The determination of IC₅₀ values was performed using GraphPad Prism (GraphPad, San Diego, CA, USA). Each experiment was performed in triplicate.

4. Conclusions

In this study, amphiphilicity of novel small molecular AMP mimics was conferred by functionalising the four cholic acid derivatives with the addition of a tryptophan moiety and different cationic groups at the carboxyl terminal and the hydroxyl ends, respectively. The difference in the number of the hydroxyl groups present on the starting cholic acid scaffold allowed the preparation of mono-, di- and tri-substituted analogues with positively charged amino acids. The significance of the hydrophobic tryptophan moiety was further investigated by its replacement with two related aromatic systems. Overall, 18 novel SMAMPs were synthesised, and their antimicrobial activities were evaluated against both Gram-positive S. aureus and Gram-negative E. coli. In vitro biological studies identified that a minimal positive net charge of +2 was essential for observable antimicrobial activity. The presence of the hydrophobic tryptophan moiety enhanced the antibacterial potency against Gram-positive S. aureus, but was found to be non-essential for activity against Gram-negative E. coli. Notably, all di-substituted analogues possessed high antimicrobial activity against both Gram-positive S. aureus and Gram-negative E. coli, with analogues 17c and 17d containing lysine and ornithine residues, respectively, showing the highest antibacterial potency with MIC values of 4 μM. Furthermore, analogue 17d showed a moderate selectivity ratio against S. aureus over mammalian cells of 8.32. Glycine-valine dipeptide derivative 27 exhibited the highest antibacterial activity against Gram-negative E. coli, with an MIC of 8 μM. Furthermore, these compounds were identified as able to act via the disruption of bacterial cell membrane, similar to most AMPs, as evidenced by the observations made in negatively charged tBLMs and the dye release assay studies.