Genome-Wide Identification of NAC Transcription Factor Family in Juglans mandshurica and Their Expression Analysis during the Fruit Development and Ripening
Abstract
:1. Introduction
2. Results
2.1. Identification and Chromosomal Location of JmNAC Genes
2.2. Phylogenetic Analysis of JmNAC Genes
2.3. Gene Structure, Motif Composition and Protein Interaction Analysis of JmNAC Genes
2.4. Chromosomal Distribution and Synteny Analysis of JmNAC Genes
2.5. Syntenic Relationships of JmNAC Genes and NAC Genes of Several Different Species
2.6. Differential Expression of JmNAC Genes during Fruit Development in J. mandshurica
2.7. Validation of Expression Pattern of RNA-seq Data Using RT-qPCR
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Identification of JmNAC Genes in J. mandshurica Genome
4.3. Sequence Analysis and Gene Structural Characterisation
4.4. Chromosomal Location and Evolutionary Analysis of JmNAC Genes
4.5. Chromosomal Location and Evolutionary Analysis of JmNAC Genes
4.6. Interaction Network Construction of JmNAC Genes
4.7. Expression Analysis of JmNAC Genes during the Development and Ripening of Fruits
4.8. RNA Extraction and RT-qPCR
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
NAC | NAM, ATAF and CUC |
TFs | transcription factors |
CDS | coding sequence |
MW | molecular weight |
pI | isoelectric point |
GO | Gene Ontology |
RPKM | reads per kilobase per million |
qRT-PCR | Quantitative real-time polymerase chain reaction |
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Li, X.; Cai, K.; Pei, X.; Li, Y.; Hu, Y.; Meng, F.; Song, X.; Tigabu, M.; Ding, C.; Zhao, X. Genome-Wide Identification of NAC Transcription Factor Family in Juglans mandshurica and Their Expression Analysis during the Fruit Development and Ripening. Int. J. Mol. Sci. 2021, 22, 12414. https://doi.org/10.3390/ijms222212414
Li X, Cai K, Pei X, Li Y, Hu Y, Meng F, Song X, Tigabu M, Ding C, Zhao X. Genome-Wide Identification of NAC Transcription Factor Family in Juglans mandshurica and Their Expression Analysis during the Fruit Development and Ripening. International Journal of Molecular Sciences. 2021; 22(22):12414. https://doi.org/10.3390/ijms222212414
Chicago/Turabian StyleLi, Xiang, Kewei Cai, Xiaona Pei, Yan Li, Yanbo Hu, Fanjuan Meng, Xingshun Song, Mulualem Tigabu, Changjun Ding, and Xiyang Zhao. 2021. "Genome-Wide Identification of NAC Transcription Factor Family in Juglans mandshurica and Their Expression Analysis during the Fruit Development and Ripening" International Journal of Molecular Sciences 22, no. 22: 12414. https://doi.org/10.3390/ijms222212414