Loss of the R2R3 MYB Transcription Factor RsMYB1 Shapes Anthocyanin Biosynthesis and Accumulation in Raphanus sativus
Abstract
:1. Introduction
2. Results
2.1. Anthocyanin Accumulation Determines the Red and Purple Color of Radish Roots
2.2. Anthocyanin Biosynthetic Genes Are Highly Expressed in Red and Purple Radish Taproots
2.3. White Taproot Radish Cultivars Harbor a Variant RsMYB1 Allele
2.4. Subcellular Localization Analysis of RsMYB1Short, RsMYB1Full, and RsTT8
2.5. Frame Shift of RsMYB1 Affects Its Interaction with RsTT8
2.6. RsMYB1Short Is a Loss of Function Allele for Anthocyanin Biosynthesis
2.7. RsMYB1Short Cannot Transactivate the RsCHS and RsDFR Promoters
2.8. Additional Unknown Factors Influence Anthocyanin Biosynthesis in Radish Taproots
3. Discussion
3.1. Spatial Expression of RsMYB1 and RsTT8 Affects Anthocyanin Accumulation in Radish Taproot
3.2. The Allelic Variant RsMYB1Short Lack of Function in Anthocyanin Biosynthesis
4. Materials and Methods
4.1. Plant Materials
4.2. RNA Extraction, cDNA Synthesis, and Genomic DNA Isolation
4.3. Measurement of Total Anthocyanin Contents
4.4. RT-qPCR Analysis
4.5. Gene Cloning and Sequence Analysis
4.6. Subcellular Localization Assay
4.7. Transactivation and Yeast Two-Hybrid (Y2H) Assays
4.8. In Planta Assays of RsMYB1 Function
4.9. Promoter Activation Assay
4.10. Molecular Marker for Discrimination of Radish Skin Color
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kim, D.-H.; Lee, J.; Rhee, J.; Lee, J.-Y.; Lim, S.-H. Loss of the R2R3 MYB Transcription Factor RsMYB1 Shapes Anthocyanin Biosynthesis and Accumulation in Raphanus sativus. Int. J. Mol. Sci. 2021, 22, 10927. https://doi.org/10.3390/ijms222010927
Kim D-H, Lee J, Rhee J, Lee J-Y, Lim S-H. Loss of the R2R3 MYB Transcription Factor RsMYB1 Shapes Anthocyanin Biosynthesis and Accumulation in Raphanus sativus. International Journal of Molecular Sciences. 2021; 22(20):10927. https://doi.org/10.3390/ijms222010927
Chicago/Turabian StyleKim, Da-Hye, Jundae Lee, JuHee Rhee, Jong-Yeol Lee, and Sun-Hyung Lim. 2021. "Loss of the R2R3 MYB Transcription Factor RsMYB1 Shapes Anthocyanin Biosynthesis and Accumulation in Raphanus sativus" International Journal of Molecular Sciences 22, no. 20: 10927. https://doi.org/10.3390/ijms222010927