7-HYB, a Phenolic Compound Isolated from Myristica fragrans Houtt Increases Cell Migration, Osteoblast Differentiation, and Mineralization through BMP2 and β-catenin Signaling
Abstract
:1. Introduction
2. Results
2.1. 7-HYB Has No Effect on the Cell Toxicity in Preosteoblasts
2.2. 7-HYB Promotes the Early Osteoblast Differentiation of Preosteoblasts
2.3. 7-HYB Enhances the Late Osteoblast Differentiation of Preosteoblasts
2.4. 7-HYB Increases Cell Migration in Osteoblast Differentiation of Preosteoblasts
2.5. 7-HYB Stimulates BMP2-Smad1/5/8-RUNX2 and β-catenin Signaling in Osteoblast Differentiation
2.6. 7-HYB Does Not Affect the Cell Toxicity in Bone Marrow Macrophages (BMMs), Premature Osteoclasts, and Mature Osteoclasts
2.7. 7-HYB Has No Biological Activities on TRAP-Positive Multinucleated Osteoclasts (MNCs) and Gene Expression in RANKL-Induced Osteoclastogenesis
3. Discussion
4. Materials and Methods
4.1. Extraction and Isolation of 7-((7S)-8′-(benzo[3′,4′]dioxol-1′-yl)-7-hydroxypropyl)benzene-2,4-diol
4.2. ((7S)-8′-(benzo[3′,4′]dioxol-1′-yl)-7-hydroxypropyl)benzene-2,4-diol (7-HYB)
4.3. Nuclear Magnetic Resonance (NMR)
4.4. Culture of MC3T3E-1 Preosteoblasts, and Differentiation of Osteoblasts
4.5. MTT Assay
4.6. Western Blot Analysis
4.7. Cell Migration Assay
4.8. Alkaline Phosphatase (ALP) Staining Assay
4.9. ALP Activity Assay
4.10. Alizarin Red S (ARS) Staining
4.11. Immunocytochemistry
4.12. Live Subject Statement
4.13. Culture of Bone Marrow Macrophages, and Osteoclast Differentiation
4.14. Tartrate-Resistant Acid Phosphatase (TRAP) Staining
4.15. Quantitative Real-Time Polymerase Chain Reaction (PCR) Analysis
4.16. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
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Park, K.-R.; Kwon, Y.-J.; Park, J.-E.; Yun, H.-M. 7-HYB, a Phenolic Compound Isolated from Myristica fragrans Houtt Increases Cell Migration, Osteoblast Differentiation, and Mineralization through BMP2 and β-catenin Signaling. Int. J. Mol. Sci. 2020, 21, 8059. https://doi.org/10.3390/ijms21218059
Park K-R, Kwon Y-J, Park J-E, Yun H-M. 7-HYB, a Phenolic Compound Isolated from Myristica fragrans Houtt Increases Cell Migration, Osteoblast Differentiation, and Mineralization through BMP2 and β-catenin Signaling. International Journal of Molecular Sciences. 2020; 21(21):8059. https://doi.org/10.3390/ijms21218059
Chicago/Turabian StylePark, Kyung-Ran, Yoon-Ju Kwon, Ji-Eun Park, and Hyung-Mun Yun. 2020. "7-HYB, a Phenolic Compound Isolated from Myristica fragrans Houtt Increases Cell Migration, Osteoblast Differentiation, and Mineralization through BMP2 and β-catenin Signaling" International Journal of Molecular Sciences 21, no. 21: 8059. https://doi.org/10.3390/ijms21218059