The Mechanism of Bisdemethoxycurcumin Enhances Conventional Antibiotics against Methicillin-Resistant Staphylococcus aureus
Abstract
:1. Introduction
2. Results
2.1. Antimicrobial Susceptibility Testing and Synergic Effect
2.2. Time–Kill Curve Assay
2.3. Antimicrobial Activity with the ATPase Inhibitor and the Membrane-Permeabilizing Agent
2.4. Expression of PBP2a in Methicillin-Resistant Staphylococcus aureus (MRSA)
2.5. BDMC Represses the Transcription of MecA, BlaZ, BlaR1, and MecR1 in S. aureus
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Bacterial Strains and Growth Medium
4.3. Susceptibility Testing of BDMC with Antibiotics
4.4. Time–Kill Curve Assay
4.5. Antibacterial Activity of BDMC in the Presence of ATPase Inhibitor or the Membrane-Permeabilizing Agent
4.6. Western Blot Analysis
4.7. Reverse Transcription and qRT-PCR
4.8. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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Strains | MIC (μg/mL) | |||
---|---|---|---|---|
BDMC | GT | AMP | OXA | |
ATCC 33591 | 7.8 | 3.9 | 62.5 | 250 |
CCARM 3090 | 7.8 | 62.5 | 31.3 | 125 |
CCARM 3091 | 7.8 | 250 | 62.5 | 1000 |
CCARM 3095 | 15.6 | 125 | 31.3 | 250 |
CCARM 3102 | 7.8 | 125 | 15.6 | 250 |
DPS-1 | 7.8 | 125 | 62.5 | 250 |
S. aureus Strains | Combination with GT | Combination with AMP | Combination with OXA | ||||||
---|---|---|---|---|---|---|---|---|---|
Fold | FICI | Interpretation | Fold | FICI | Interpretation | Fold | FICI | Interpretation | |
ATCC 33591 | 4 | 0.75 | partial synergy | 4 | 0.75 | partial synergy | 2 | 0.51 | partial synergy |
CCARM 3090 | 8 | 0.2 | synergy | 4 | 0.75 | partial synergy | 4 | 0.75 | partial synergy |
CCARM 3091 | 8 | 0.3 | synergy | 2 | 1 | additive effect | 4 | 0.75 | partial synergy |
CCARM 3095 | 8 | 0.36 | synergy | 4 | 0.75 | partial synergy | 4 | 0.62 | partial synergy |
CCARM 3102 | 4 | 0.5 | synergy | 8 | 0.62 | partial synergy | 4 | 0.62 | partial synergy |
DPS-1 | 16 | 0.1 | synergy | 2 | 0.51 | partial synergy | 2 | 1 | additive effect |
Primer | Sequence (5′-3′) |
---|---|
16S RNA | F:ACTCCTACGGGAGGCAGCAG |
R:ATTACCGCGGCTGCTGG | |
mecA | F:CAATGCCAAAATCTCAGGTAAAGTG |
R:AACCATCGTTACGGATTGCTTC | |
mecR1 | F:GTGCTCGTCTCCACGTTAATTCCA |
R:GACTAACCGAAGAAGTCGTGTCAG | |
blaR1 | F:CACTATTCTCAGAATGACTTGGT |
R:TGCATAATTCTCTTACTGTCATG | |
blaZ | F:GCTTTAAAAGAACTTATTGAGGCTTC |
R:CCACCGATYTCKTTTATAATTT |
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Wang, S.; Kim, M.-C.; Kang, O.-H.; Kwon, D.-Y. The Mechanism of Bisdemethoxycurcumin Enhances Conventional Antibiotics against Methicillin-Resistant Staphylococcus aureus. Int. J. Mol. Sci. 2020, 21, 7945. https://doi.org/10.3390/ijms21217945
Wang S, Kim M-C, Kang O-H, Kwon D-Y. The Mechanism of Bisdemethoxycurcumin Enhances Conventional Antibiotics against Methicillin-Resistant Staphylococcus aureus. International Journal of Molecular Sciences. 2020; 21(21):7945. https://doi.org/10.3390/ijms21217945
Chicago/Turabian StyleWang, Shu, Min-Chul Kim, Ok-Hwa Kang, and Dong-Yeul Kwon. 2020. "The Mechanism of Bisdemethoxycurcumin Enhances Conventional Antibiotics against Methicillin-Resistant Staphylococcus aureus" International Journal of Molecular Sciences 21, no. 21: 7945. https://doi.org/10.3390/ijms21217945