Copper(II) Binding by the Earliest Vertebrate Gonadotropin-Releasing Hormone, the Type II Isoform, Suggests an Ancient Role for the Metal
Abstract
:1. Introduction
2. Results and Discussion
2.1. GnRH-II Binds Copper with a 1:1 Stoichiometry in a Four-Nitrogen Environment
2.2. Histidine Amino-Acids Are Highly Conserved in Vertebrate GnRH-II
3. Materials and Methods
3.1. Materials
3.2. Mass Spectroscopy (MS)
3.3. Electronic Spectroscopy
3.4. Fluorescence Spectroscopy
3.5. Electron Paramagnetic Resonance (EPR)
3.6. Circular Dichroism (CD) Spectroscopy
3.7. Phylogenetic Analysis
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Parameter | Value |
---|---|
gi (i = x, y, z) | 2.067, 2.067, 2.222 |
Ai (63Cu) (×10−4 cm−1) | 10.0, 10.0, 196.8 |
Linewidth (×10−4 cm−1) | 4.67 |
g-straini | 0.052, 0.058, 0.086 |
A-straini (×10−4 cm−1) | 11.7, 11.7, 3.3 |
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Peacey, L.; Peacey, C.; Gutzinger, A.; Jones, C.E. Copper(II) Binding by the Earliest Vertebrate Gonadotropin-Releasing Hormone, the Type II Isoform, Suggests an Ancient Role for the Metal. Int. J. Mol. Sci. 2020, 21, 7900. https://doi.org/10.3390/ijms21217900
Peacey L, Peacey C, Gutzinger A, Jones CE. Copper(II) Binding by the Earliest Vertebrate Gonadotropin-Releasing Hormone, the Type II Isoform, Suggests an Ancient Role for the Metal. International Journal of Molecular Sciences. 2020; 21(21):7900. https://doi.org/10.3390/ijms21217900
Chicago/Turabian StylePeacey, Lorraine, Charlotte Peacey, Adele Gutzinger, and Christopher E. Jones. 2020. "Copper(II) Binding by the Earliest Vertebrate Gonadotropin-Releasing Hormone, the Type II Isoform, Suggests an Ancient Role for the Metal" International Journal of Molecular Sciences 21, no. 21: 7900. https://doi.org/10.3390/ijms21217900