Next Article in Journal
GmIDL2a and GmIDL4a, Encoding the Inflorescence Deficient in Abscission-Like Protein, Are Involved in Soybean Cell Wall Degradation during Lateral Root Emergence
Next Article in Special Issue
TGF-Beta Signaling in Bone with Chronic Kidney Disease
Previous Article in Journal
Heme Oxygenase 1 in the Nervous System: Does It Favor Neuronal Cell Survival or Induce Neurodegeneration?
Previous Article in Special Issue
TGF-β Signaling-Related Genes and Thoracic Aortic Aneurysms and Dissections
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 19
Issue 8
10.3390/ijms19082261
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Review

Reevaluation of Pluripotent Cytokine TGF-β3 in Immunity

by
Toshihiko Komai
1,
Tomohisa Okamura
1,2,3,*,
Mariko Inoue
1,
Kazuhiko Yamamoto
1,3,4 and
Keishi Fujio
1,*
1
Department of Allergy and Rheumatology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-8655, Japan
2
Department of Functional Genomics and Immunological Diseases, Graduate School of Medicine, The University of Tokyo, Tokyo 113-8655, Japan
3
Max Planck-The University of Tokyo Center for Integrative Inflammology, The University of Tokyo, Tokyo 153-8505, Japan
4
Laboratory for Autoimmune Diseases, Center for Integrative Medical Sciences, RIKEN, Kanagawa 230-0045, Japan
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(8), 2261; https://doi.org/10.3390/ijms19082261
Submission received: 10 July 2018 / Accepted: 28 July 2018 / Published: 1 August 2018
(This article belongs to the Special Issue TGF-Beta Super Family Signaling)
Browse Figures
Versions Notes

Abstract

:
Transforming growth factor (TGF)-βs are pluripotent cytokines with stimulatory and inhibitory properties for multiple types of immune cells. Analyses of genetic knockouts of each isoform of TGF-β have revealed differing expression patterns and distinct roles for the three mammalian isoforms of TGF-β. Considerable effort has been focused on understanding the molecular mechanisms of TGF-β1-mediated immune regulation, given its pivotal role in prohibiting systemic autoimmune disease. In recent years, functional similarities and differences between the TGF-β isoforms have delineated their distinct roles in the development of immunopathology and immune tolerance, with increased recent attention being focused on TGF-β3. In addition to the characteristic properties of each TGF-β isoform, recent progress has identified determinants of context-dependent functionality, including various cellular targets, cytokine concentrations, tissue microenvironments, and cytokine synergy, which combine to shape the physiological and pathophysiological roles of the TGF-βs in immunity. Controlling TGF-β production and signaling is being tested as a novel therapeutic strategy in multiple clinical trials for several human diseases. This review highlights advances in the understanding of the cellular sources, activation processes, contextual determinants, and immunological roles of TGF-β3 with comparisons to other TGF-β isoforms.

1. Introduction

The transforming growth factor (TGF)-β superfamily comprises more than 40 members, including the TGF-βs, bone morphogenetic proteins (BMPs), growth and differentiation factors (GDFs), activins, and nodals [1,2,3]. TGF-β superfamily members share several biological functions, but also possess specific or opposite functions under some conditions [1].
The TGF-βs are involved in a variety of biological functions in cellular activities, fibrosis, and immune responses, in addition to their crucial roles in tissue homeostasis [1]. Three mammalian TGF-β isoforms (TGF-β1, TGF-β2, and TGF-β3) have been identified, with 70–82% amino acid homology [4]. TGF-β1 has been extensively investigated in immunity, because TGF-β1 is expressed predominantly by various immune cells [5], and deficiency of Tgfb1 results in fatal systemic autoimmune disease [6,7]. TGF-β2, however, is thought to play an insignificant role in the immune system due to its low expression in immune cells [8]. Recent accumulating evidence on the expression and function of TGF-β3 in immunity has revealed similarities and differences between TGF-β1 and TGF-β3 [9,10]. In this review, we focus on the molecular characteristics, immunological roles, and future possibilities of TGF-β3 in comparison to TGF-β1.

2. TGF-β Superfamily Members and Immunity

TGF-β1, a prototypic TGF-β superfamily cytokine, was discovered in the early 1980s as an autocrine factor secreted by neoplastic cells to promote their transformation [11,12,13]. Mature TGF-β1 forms a disulphide-linked dimer of two identical chains derived from the C-terminus by proteolytic cleavage [14]. A number of proteins that share sequence similarity have been assigned to the TGF-β superfamily [14], and each TGF-β superfamily ligand signals by binding heteromeric complexes of transmembrane type I receptors and type II serine/threonine kinase receptors [1,15]. TGF-β superfamily members can be further subdivided into TGF-βs (TGF-β1, -β2, -β3), activins/inhibins/nodal proteins, and BMPs [15]. In this section, we discuss TGF-β superfamily members other than the TGF-βs and their impact on immunity.
Activins exist as either homo- or hetero-dimers of β-subunits, and the neonatal lethality with craniofacial abnormalities in activin-βA knockout mice indicates the importance of activin A during mammalian development [16]. Previous analyses demonstrate that activin A suppresses the generation and survival of B cells [17], cytokine and chemokine production by dendritic cells (DCs) [18], and the proliferation of thymocytes [19] and peripheral blood lymphocytes [20]. In T cells, activin A promotes TGF-β1-depdendent conversion of CD4+CD25− T cells into forkhead box P3 (Foxp3)-expressing regulatory T cells (Tregs) [21], which play critical roles in immune homeostasis [22,23], in addition to inducing antigen-specific Tregs [24]. Although these findings suggest that activin A functions as a potent anti-inflammatory cytokine, activin A also has pro-inflammatory effects, and its systemic role in vivo has not been clearly demonstrated [25]. It has been reported that activin A is increased in synovial fluid of rheumatoid arthritis (RA) patients [26], and that activin A induces the proliferation of fibroblast-like synoviocytes [27]. Further studies are needed in order to characterize activin A activity in vivo and its influence on disease pathogenesis in humans.
BMPs were initially discovered as a factor that induces bone formation, and a number of crucial roles in embryogenesis, development, the skeletal system, and tissue homeostasis have since been identified and assigned to the BMPs. BMP2 and BMP4 knockout mice result in embryonic lethality, and BMP1, BMP7, and BMP11 knockout mice die shortly after birth [28]. BMPs are reported to regulate diverse immune cell types, such as DCs, macrophages, natural killer (NK) cells, B cells, and T cells [1]. BMPs generally enhance anti-inflammatory activities, and BMP2 and BMP4 support thymic organogenesis [1]. Since BMPs regulate neuronal and glial lineage cells as well as impacting immune responses, BMPs are potential therapeutic targets for the neurodegenerative autoimmune disease multiple sclerosis (MS) [29]. For example, injected neuronal precursor cells ameliorate the pathologies of experimental autoimmune encephalomyelitis (EAE), an animal model of MS, in a BMP4-dependent manner [30]. Furthermore, T cell secretion of BMP2, BMP4 and BMP5 is elevated in MS patients [31]. Although BMP5 expression is increased in mesenchymal stem cells from systemic lupus erythematosus (SLE) patients [32], BMP4 and BMP5 expression in synovial tissues from osteoarthritis and RA patients [33], and BMP2 and BMP6 expression in the arthritic synovium from RA and spondyloarthropathy patients [34] were decreased. Despite the intriguing expression data from a variety of autoimmune disorders, the immunological importance of BMPs in relation to these systemic autoimmune diseases has not been clearly demonstrated [1].
Mounting recent evidence suggests that activin A and BMPs have immunological roles, but only limited studies, especially in systemic autoimmune diseases, have been reported, in contrast to the TGF-βs [1]. Further investigation of TGF-β superfamily members in immune responses is needed in order to guide future strategies to develop therapies for systemic autoimmune diseases.

3. Expression of TGF-βs in Immune Cells

Immune cells are major sources of TGF-βs for regulating immune responses [35]. Since TGF-β1 is the predominantly expressed isoform in the immune system [5], the production and function of TGF-β1 has been preferentially investigated.
Conditional deletion of Tgfb1 in T cells results in systemic autoimmune inflammation, demonstrating the fact that TGF-β1 produced by T cells helps maintain immune homeostasis [36]. Tregs are known to be one of the major producers of TGF-β1 [5,37]. Thymus-derived CD4+CD25+ Tregs produce high levels of TGF-β1 in a cell surface-bound form [38], and Tregs developed in the periphery, such as T regulatory 1 (Tr1) cells, secrete TGF-β1 [39,40]. Although FoxP3+ Treg-derived TGF-β1 could be redundant in Treg-mediated immune tolerance in some conditions [35,41], TGF-β plays a crucial role in Treg-cell-mediated suppression of T cells in vivo [42]. In other T cell subsets, activated C-X-C chemokine receptor type 5 (CXCR5) expressing follicular helper T (TFH) cells are reported to produce TGF-β1 [43]. CXCR5+ T cell subsets include both TFH cells and recently identified follicular regulatory T (TFR) cells [44,45]; thus, further evaluation of the cellular source of TGF-β1 among CXCR5+ T cell subsets is required.
It is well known that lymphocytes other than T cells also produce TGF-β. Lipopolysaccharide (LPS)-activated B cells produce surface TGF-β1 that exerts inhibitory effects on CD8+ T cells [46], and neuroinflammation was exacerbated when the Tgfb1 gene was conditionally deleted in B cells, highlighting the important regulatory roles of B cell-derived TGF-β1 [47]. TGF-β1 production from B cells is reduced upon co-engagement of the B cell receptor (BCR) and Toll-like receptor (TLR) 9, and the suppression of B cell-derived TGF-β1 with stimulation could lead to a breakdown in immune tolerance [48]. Another lymphocyte subset, NK cells, also produce TGF-β1 [49], which is important for anti-tumor NK cell activity [50].
Myeloid cells including macrophages, dendritic cells (DCs), mast cells, and eosinophils also produce TGF-β1. Myeloid cell-specific deletion of Tgfb1 attenuated progression of heterotropic ossification induced by Achilles tendon puncture [51]. More specifically, LPS-stimulated macrophages produce TGF-β1, which induces B cells to secrete IgA antibody [52], and bone marrow-derived immature DCs rather than mature DCs produce TGF-β1 [53]. Mast cells are unique in that they co-secrete latent TGF-β1 and the activating enzyme chymase 1 that activates TGF-β1 [54]. In granulocytes, TGF-β1 derived from tissue-resident eosinophils in airway [55] and intestine [56] plays important roles for homeostasis in peripheral tissues [57]. Furthermore, recently identified innate lymphoid cells (ILCs) resident in the intestine produce TGF-β1, which expands regulatory ILCs during inflammation in an autocrine manner [58].
In contrast to TGF-β1, according to the literature currently available, the amount of TGF-β2 in the immune system is negligible, and the production of TGF-β3 from immune cells has only recently been recognized [9,10]. TGF-β3 mRNA is reported to be expressed in lymphocytes such as CD4+ T cells, CD8+ T cells, γδT cells, and B cells [10]. At the protein level, TGF-β3 is highly produced from CD4+CD25−LAG3+ Tregs (LAG3+ Tregs), with lesser amounts of TGF-β3 produced by Th1 cells and Th17 cells [59]. We have previously reported that LAG3+ Tregs suppress systemic humoral immune responses in a TGF-β3-dependent manner [59], and that TGF-β3 production from LAG3+ Tregs is significantly reduced when the expression of transcriptional factors early growth response gene 2 (Egr2) and Egr3 is deficient or if Fas is mutated [59,60]. Another group reported that Egr2-deficient mice develop lupus-like autoimmune disease [61]. We previously verified that polymorphisms in EGR2 are associated with susceptibility to SLE [62], and that Egr2 is characteristically expressed in LAG3+ Tregs [63,64], which have the potential to ameliorate lupus pathology [59]. TGF-β3’s role as a regulatory molecule of LAG3+ Tregs and its inhibitory effect on systemic autoimmune diseases, including SLE, warrants further investigation.

4. Synthesis of TGF-β3

The biological activities of TGF-βs are regulated at multiple steps, including synthesis, proteolytic processing, secretion, and activation [65]. All three TGF-β isoforms are initially synthesized as an inactive pre-pro-TGF-β precursor, and the removal of the signal peptide, homo dimerization of pro-TGF-β, and subsequent cleavage by furin convertase forms a small latent complex in which the mature TGF-β ligand and its latency-associated peptide (LAP) are non-covalently connected [65]. The LAP renders the biological activities of TGF-βs latent by shielding the receptor-binding epitopes in the mature ligand [65,66]. Subsequently, the small latent complex covalently binds to latent TGF-β binding protein (LTBP) to form the large latent complex, which interacts with components of the extracellular matrix (ECM) [10,65].
LTBPs are multi-domain glycoproteins that interact with fibrillin microfibrils [67]. Of the four isoforms of LTBP, LTBP-1 and LTBP-3 bind to all three TGF-β isoforms, whereas LTBP-2 does not bind the LAP of any TGF-β isoform, and LTBP-4 is supposed to interact only with the TGF-β1 LAP [68]. The disulfide bond between LAP and LTBP is an bridge between cysteine residues in the LAP and LTBP [69]. Genetically mutated mice with the cysteine residue in the LAP changed to serine exhibit systemic inflammation, although not as strong as Tgfb1 knockout mice, and reduced serum TGF-β1 levels; thus, the association of latent TGF-β1 with LTBP is important for extracellular TGF-β1 activity [70]. However, the specific binding of LTBP-1 or LTBP-3 to each TGF-β isoform has not been clearly demonstrated [71]. Recently, we reported that secretion of TGF-β3 by LAG3+ Tregs is regulated by LTBP-3 in T cells (Figure 1) in a murine model of lupus [60]. Additionally, deletion of Egr2 and/or Egr3 decreases Ltbp3 expression [60]; thus Egr2/Egr3 could be critical for TGF-β3 synthesis. Further investigation of the role of LTBPs in the secretory mechanics of TGF-β would lead to insightful findings in immune responses.
Activation of latent complexes of TGF-βs is essential in order to exert their biological functions [35,42]. TGF-β1 and TGF-β3 have an integrin-binding arginylglycylaspartic acid (RGD) motif, which is recognized by αv integrins and is required for integrin-mediated activation [71,72]. One groups showed that genetically modified mice harboring a nonfunctional variant of the RGD sequence in the Tgfb1 gene develop fatal multiorgan inflammation identical to Tgfb1 knockout mice [73]. Among the members of the integrin receptor family, integrin αvβ6 and αvβ8 have the capacity to bind and activate pro-TGF-β1 and pro-TGF-β3 [74], and the combined genetic loss of αvβ6 and αvβ8 integrins reproduces the phenotypes of Tgfb1 and Tgfb3 knockout mice [75]. Conditional deletion of the Igtb8 gene encoding integrin β8 in leukocytes results in systemic inflammation with autoantibodies [76]. Further, deficiency of integrin αvβ8 in Tregs, which display less active TGF-β levels, abrogates their suppressive abilities on T cell responses [42]. The elaborate machinery of TGF-β synthesis is carefully regulated by the immune system, and the recent findings of TGF-β isoform-specific function suggest the importance of investigating further TGF-β isoform-specific synthesis mechanisms.

5. TGF-β3 Signal Transduction

TGF-βs signal through a hetero-tetrameric receptor complex composed of two type I receptors and two type II receptors [77]. The constitutively active cytoplasmic domain of TGF-β type II receptors (TβRII) phosphorylates type I receptors on serine and threonine residues in response to the binding of mature TGF-βs, after which the activated type I receptors canonically phosphorylate Smad proteins [77,78]. TβRII interacts with TGF-β type I receptors (TβRI), also called activin receptor like kinase (ALK)-5, which typically induces the phosphorylation of Smad2 and Smad3, and also interacts with ALK-1, which typically induces the phosphorylation of Smad1 and Smad5 [79,80]. In endothelial cells, ALK1 antagonizes ALK5-mediated Smad2/Smad3 signaling, and the ratio of ALK5 to ALK1 determines the responsiveness to TGF-βs [79,81]. Further, type III receptors, such as endoglin and betaglycan, regulate the access of TGF-βs to the type I and type II receptors [82]. Although TGF-β1 and TGF-β3 can interact with type II receptors even without type I receptors, TGF-β2 interacts very weakly with type II receptors, and type I or type III receptors are required for binding [80,82]. In association with TβRII, TGF-β1 and TGF-β3, but not TGF-β2, interact with endoglin [82,83].
Like TGF-β1 [84], the initial report demonstrated that TGF-β3 dominantly drives Smad2-dependent ALK-5 signaling in palatal fusion [85]. During palatogenesis, conditional deletion of Trim33 and Smad4 in epithelium in concert with TGF-β activated kinase-1 (Tak1) inhibition phenocopied the palate defects observed in epithelium-specific Tgfb3-deficient mice; thus TGF-β3 is supposed to transduce signals via both canonical Smad-dependent and non-canonical Smad-independent signaling [86]. In human B cells, we recently found that TGF-β3 induces phosphorylation of Smad1/5 along with Smad2 and Smad3 [87], as previously reported in TGF-β1-treated B cells [88]. The differential signal transduction observed for TGF-β1 and TGF-β3 is linked to different downstream functions, at least for CD4+ T cells. TGF-β3 produces highly pathogenic Th17 cells and induces more phosphorylation of Smad1 and Smad5, but less Smad2 and Smad3 phosphorylation, than TGF-β1 [89], although further studies of the different downstream signaling pathways of TGF-β3 and TGF-β1 are warranted.

6. TGF-β3 in Immune Responses

TGF-βs are pluripotent cytokines with both pro-inflammatory and anti-inflammatory effects, depending upon specific immune contexts such as cellular targets, cytokine concentrations, tissue microenvironments, and cytokine synergy [9,10,77,90]. In contrast to the previous reports describing a variety of immunological roles for TGF-β1, a direct immunological role for TGF-β3 has not been described in detail until recently. Although Tgfb1-knockout mice develop fatal systemic autoimmune inflammation in heart, lungs, pancreas, colon and salivary glands [6,7], the immunological abnormalities in Tgfb3-knockout mice have not been reported due to death shortly after birth due to cleft palate formation and impaired lung development in Tgfb3-knockout mice [91,92].

6.1. Pro-Inflammatory Roles of TGF-β3

Similar to TGF-β1 [5,9,37], TGF-β3 has pro-inflammatory effects. Derepression of TGF-βs, especially Tgfb3 mRNA, results in the accumulation of Th17 cells and Foxp3+ T cells in T cell-specific deletion of tripartite motif protein 28 (Trim28) [93]. Further analyses indicate an isoform-specific function of TGF-βs in Th17 cells in that a combination of TGF-β3 and IL-6 induces highly pathogenic Th17 cells, compared to the Th17 cells induced by TGF-β1 plus IL-6 [89].
In B cells, low concentrations of TGF-β1 enhance T cell-dependent sheep erythrocyte-induced B cell proliferation [94]. In addition, TGF-β1 enhances IgG2b and IgA production from LPS-induced B cells [95]. A direct pro-inflammatory role of TGF-β3 has not been reported, but our latest research indicates that antibody production in LPS-stimulated B cells is enhanced with TGF-β3 [96]. TGF-β concentration is one of the determinants for its pro-inflammatory effects. TGF-β1 shows concentration-dependent bifunctional effects in B cells [94], and we have verified that TGF-β3 also has concentration-dependent bifunctional effects [87,96]. Although a combination of TGF-β1 and IL-21 generates mucosal-homing IgA-secreting plasmablasts [43], a role for TGF-β3 in mucosal immunity has not been demonstrated. Since TGF-βs show context-dependent pro-inflammatory effects on B cells, different concentrations and a combination of cytokines should be utilized when investigating their immunoregulatory roles.

6.2. Anti-Inflammatory Roles of TGF-β3

Unlike TGF-β1 [5,37], direct anti-inflammatory roles for TGF-β3 in vivo had not been clearly documented until our recent reports. Targeted recombination of the coding sequence of mature TGF-β1 with a sequence from TGF-β3 partially prevented autoimmune diseases caused by TGF-β1 deficiency, indicating that TGF-β3 is not fully interchangeable with TGF-β1 and that they have distinct immunoregulatory roles [97]. In T cells, TGF-β3 inhibits the differentiation of Foxp3-expressing CD4+ T cells [98]. Moreover, treatment with a TGF-β3 neutralizing antibody prevented TGF-β3-expressing LAG3+ Treg-mediated suppression [59]. Although TGF-β1 is known to inhibit the differentiation of Th1 [99] and Th2 cells [100], and suppress the activation of NK cells [101] and macrophages [102], the effects of TGF-β3 on these cell subsets has not yet been described.
B cell-specific deletion of TβRII results in B cell hyperresponsiveness and virtually complete serum IgA deficiency, indicating that TGF-βs could have a regulatory role in T cell-independent B cell homeostasis [103]. TGF-β1 inhibits anti-IgM-simulated B cells by suppressing the phosphorylation of spleen tyrosine kinase (Syk), and inhibits IL-4-stimulated B cells by suppressing the phosphorylation of signal transducer and activator of transcription-6 (STAT6) [104]. Similar to TGF-β1, TGF-β3 inhibits B cell-proliferation and antibody production stimulated by anti-IgM, IL-4, or anti-CD40 plus IL-4 by suppressing the phosphorylation of Syk, STAT6, or NF-κB p65, respectively [59]. High-affinity antibodies are generated through the interaction of TFH cells with germinal center B (GCB) cells [105,106], and TGF-β3, as well as TGF-β1, suppresses GCB cells in vitro [60]. Lastly, systemic administration of TGF-β3-expressing vectors, but not TGF-β1-expressing vectors, inhibited T cell-dependent humoral immune responses by suppressing the development of GCB cells [96].
In contrast, both TGF-β1 and TGF-β3 enhance antibody production by LPS-stimulated B cells. We recently reported that TGF-βs exhibit inhibitory effects against LPS-stimulated B cells in the presence of IL-10 [96], which is a comprehensive inhibitory cytokines [107]. Cytokine synergy is a phenomenon of greater effects than the sum of its parts [108], and our report is the first to propose inhibitory cytokine synergy (ICS) [96], which is defined as a coordinated inhibitory ability of two or more cytokines, but not either cytokine alone. The ICS effects of TGF-β3 and IL-10 regulate TLR-mediated humoral immune responses both in vitro and in vivo by suppressing mammalian target of rapamycin (mTOR) signaling. The inhibition of mTOR signaling in B cells by ICS effects further suppresses cellular metabolism, including glycolysis and mitochondrial oxidative phosphorylation (Figure 2). TGF-β3 ameliorates IL-10-sufficient lupus-prone MRL/Faslpr/lpr (MRL/lpr) mice [59], but the requirement of both TGF-β3 and IL-10 in the regulation of other lupus models induced with a TLR7 agonist [109] implies the importance of considering ICS when designing future therapeutic strategies. Irrespective of the presence of IL-10, high concentrations of TGF-β3, like TGF-β1, inhibit proliferation, antibody production, and the differentiation of human B cells activated with various stimuli [87]. However, a physiological concentration of TGF-β3 enhances activated human B cells, which notably requires the presence of IL-10 [96]. Collectively, TGF-β3 has the potential to regulate systemic autoimmune diseases by inhibiting B cells. Context-dependent functionality of TGF-β3, like TGF-β1, could be an obstacle for clinical application and adequate inhibitory conditions in immune responses should be further investigated.

7. TGF-β3 and Human Diseases

Recent advances in understanding the biological functions of TGF-β3 are incrementally uncovering the roles of TGF-β3 in human diseases. As mentioned above, Tgfb3 knockout mice die perinatally, and immunological abnormalities have not been reported [91,92]. In humans, TGFB3 mutations are associated with cardiovascular involvement, including aortic aneurysm, dissection, mitral valve diseases, and systemic features overlapping with Loeys–Dietz, Shprintzen–Goldberd and Marfan syndromes [110]. Although several family members with autoimmune features, including HLA-B27-positive spondyloarthritis, Graves’s disease, and celiac disease [110] have been identified, a linkage between TGFB3 mutations and autoimmunity has not been studied in detail.
TGF-βs are thought to be central mediators of systemic sclerosis (SSc) pathogenesis, because of the pleiotropic effects of TGF-βs in fibrosis, inflammation, and vascular biology [111]. Immunohistochemical analyses indicate that the expression of all three isoforms of TGF-βs are increased in skin from SSc patients [111]. In addition, the expression of TGF-β-regulated genes, such as cartilage oligomeric matrix protein (COMP) and collagen type V α2 (COL5A2), is upregulated in lungs from SSc patients [112]. Although no significant differences in total serum TGF-β1 levels between SSc patients and healthy controls have been observed, serum-active TGF-β1 levels in diffuse cutaneous SSc patients are negatively correlated with skin score and lower than active TGF-β1 levels in limited cutaneous SSc patients and healthy controls [113]. In animal models with blocked TGF-β signaling, inhibition of ALK5 attenuates bleomycin-induced pulmonary fibrosis [114], and LAP prevents sclerodermatous graft-versus-host disease [115]. Metelimumab, which is a human monoclonal antibody that specifically targets TGF-β1, failed in phase I/II trials for SSc [116]. In contrast, fresolimumab, which is specific for TGF-β1 with high affinity in addition to TGF-β2 and TGF-β3, benefited SSc patients, and further studies for the safety and longer-term use effects are expected [117]. A genome-wide association study (GWAS) of SSc patients in African Americans recently identified TGFB3 as a SSc susceptibility gene [118]. These results collectively suggest that TGF-β3 could be an important mediator and drug target for SSc.
In SLE, the roles of TGF-βs are expected to be protective. For examples, TGF-β1 ameliorates murine lupus [119,120], and serum TGF-β1 concentrations are low in active SLE patients [121,122]. Additionally, an impaired response to a TGF-β1-mediated anti-proliferative effect against peripheral blood mononuclear cells from active SLE patients has been reported [123]. In relation to TGF-β3, mice with Egr2/Egr3 deficiency, which leads to the reduction of TGF-β3 secretion from LAG3+ Tregs, develop lupus-like diseases [60]. Furthermore, therapeutic effects of TGF-β3 in murine lupus [59,96], and the reduction of TGF-β3-secreting LAG3+ Tregs in SLE patients [59] imply potential preventive roles of TGF-β3 in SLE.
TGF-β1 and TGF-β3, in combination with other cytokines, regulate both encephalitogenic T cells and regulatory T cells [9,89], although precise roles for TGF-βs in MS have not been elucidated. Mice with T cell-specific deletion of Tgfb1 are resistant to EAE and exhibit less Th17 cell differentiation [41], although the in vivo roles of TGF-β3 in a mouse model of EAE have not been reported. Since CD4+ T cells from MS patients show reduced levels of TGF-β signaling components [124], the initial failure of a clinical trial using TGF-β2 for MS [125] could be due to the lowered responsiveness of TGF-βs in the pathologies of MS. Further evaluations of TGF-β signaling in MS that incorporates recent understandings of TGF-β3’s impact on pathogenic Th17 cells are to be expected [126].
Targeting TGF-β pathways as a therapeutic strategy has been clinically applied outside the field of immunology. TGF-β is considered to be a critical regulator of fibrosis; TGF-β1 and TGF-β2 have pro-fibrotic effects and promote fibroplasia, whereas TGF-β3 exerts anti-fibrotic effects and reduces scar formation [9]. A humanized monoclonal antibody directed against integrin αvβ6, which activates pro-TGF-β1 and pro-TGF-β3 [74], is currently in phase II trials in patients with idiopathic pulmonary fibrosis [127]. Although human recombinant TGF-β3, avotermin, failed in phase III clinical trials [128], avotermin improved wound healing without serious adverse events in phase I/II clinical trials for a prophylactic anti-scarring therapy [129]. In the field of oncology, regulation of TGF-β activity in tumor microenvironments and targeting TGF-β signaling as a novel immunotherapy has been in the news [130,131]. The TβRI-specific inhibitors galunisertib and fresolimumab, in combination with other drugs, have been in clinical trials for a variety of tumors [130]. The systemic effects, immunological changes, and safety with these novel medications that modulate TGF-β signaling provide insightful information for future therapeutic strategies for systemic autoimmune diseases.

8. Concluding Remarks

The pluripotent TGF-β cytokines have been investigated in a variety of fields. Although the three isoforms of TGF-β each have distinct biological roles, TGF-β1 has been paid particular attention regarding its ability to regulate immunity. Recent identification of pro- and anti-inflammatory roles for TGF-β3 [9,10] highlights the importance of the evaluation of TGF-β isoform-specific immunological roles. Since TGF-β3—like TGF-β1—has a context-dependent functionality in immunity [90,96], considering concentrations and combination with other cytokines is important when evaluating the pathogenic and pathoprotective roles of TGF-β3. Investigations of TGF-β3 by conditionally mutated mouse models, together with evaluations of the physiological and pathological roles of TGF-β3 in humans, would be helpful for further progress in assessing TGF-β3-mediated immune responses.

Author Contributions

T.K., T.O., M.I., K.Y., and K.F. contributed to writing the manuscript.

Funding

This work was supported by grants from the Japan Society for the Promotion of Science, and the Ministry of Education, Culture, Sports, Science and Technology of Japan (Grant Numbers JP16K09918, JP16K15510).

Conflicts of Interest

Tomohisa Okamura received financial support or fees from Chugai and Bristol-Myers Squibb (BMS). Kazuhiko Yamamoto received financial support or fees from AbbVie, Astellas, BMS, Daiichi-Sankyo, Mitsubishi Tanabe, Pfizer, Sanofi, Santen, Takeda, Teijin, Boehringer Ingelheim, Chugai, Eisai, Ono, Taisho Toyama, UCB, ImmunoFuture, Asahi Kasei, Janssen, and NIPPON KAYAKU. Keishi Fujio received financial support or fees from Astellas, BMS, Daiichi-Sankyo, Mitsubishi Tanabe, Pfizer, Ayumi, Takeda, Chugai, Eisai, Taisho Toyama, UCB, Janssen, Eli Lilly and NIPPON KAYAKU. Toshihiko Komai, Tomohisa Okamura, Mariko Inoue, Kazuhiko Yamamoto, and Keishi Fujio received patent-licensing arrangements with Chugai.

References

  1. Chen, W.; Ten Dijke, P. Immunoregulation by members of the TGFβ superfamily. Nat. Rev. Immunol. 2016, 16, 723–740. [Google Scholar] [CrossRef] [PubMed]
  2. Zhao, M.; Mishra, L.; Deng, C.X. The role of TGF-β/SMAD4 signaling in cancer. Int. J. Biol. Sci. 2018, 14, 123. [Google Scholar] [CrossRef] [PubMed]
  3. Wakefield, L.M.; Hill, C.S. Beyond TGFβ: Roles of other TGFβ superfamily members in cancer. Nat. Rev. Cancer 2013, 13, 328–341. [Google Scholar] [CrossRef] [PubMed]
  4. Meng, X.; Nikolic-Paterson, D.J.; Lan, H.Y. TGF-β: The master regulator of fibrosis. Nat. Rev. Nephrol. 2016, 12, 325–338. [Google Scholar] [CrossRef] [PubMed]
  5. Li, M.O.; Wan, Y.Y.; Sanjabi, S.; Robertson, A.K.; Flavell, R.A. Transforming growth factor-β regulation of immune responses. Annu. Rev. Immunol. 2006, 24, 99–146. [Google Scholar] [CrossRef] [PubMed]
  6. Kulkarni, A.B.; Huh, C.G.; Becker, D.; Geiser, A.; Lyght, M.; Flanders, K.C.; Roberts, A.B.; Sporn, M.B.; Ward, J.M.; Karlsson, S. Transforming growth factor β1 null mutation in mice causes excessive inflammatory response and early death. Proc. Natl. Acad. Sci. USA 1993, 90, 770–774. [Google Scholar] [CrossRef] [PubMed]
  7. Shull, M.M.; Ormsby, I.; Kier, A.B.; Pawlowski, S.; Diebold, R.J.; Yin, M.; Allen, R.; Sidman, C.; Proetzel, G.; Calvin, D. Targeted disruption of the mouse transforming growth factor-β1 gene results in multifocal inflammatory disease. Nature 1992, 359, 693–699. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  8. Rubtsov, Y.P.; Rudensky, A.Y. TGFβ signalling in control of T-cell-mediated self-reactivity. Nat. Rev. Immunol. 2007, 7, 443–453. [Google Scholar] [CrossRef] [PubMed]
  9. Fujio, K.; Komai, T.; Inoue, M.; Morita, K.; Okamura, T.; Yamamoto, K. Revisiting the regulatory roles of the TGF-β family of cytokines. Autoimmun. Rev. 2016, 15, 917–922. [Google Scholar] [CrossRef] [PubMed]
  10. Okamura, T.; Morita, K.; Iwasaki, Y.; Inoue, M.; Komai, T.; Fujio, K.; Yamamoto, K. Role of TGF-β3 in the regulation of immune responses. Clin. Exp. Rheumatol. 2015, 33, 63–69. [Google Scholar]
  11. Roberts, A.B.; Anzano, M.A.; Lamb, L.C.; Smith, J.M.; Sporn, M.B. New class of transforming growth factors potentiated by epidermal growth factor: Isolation from non-neoplastic tissues. Proc. Natl. Acad. Sci. USA 1981, 78, 5339–5343. [Google Scholar] [CrossRef] [PubMed]
  12. Wrana, J.L. Signaling by the TGF-β Superfamily. Cold Spring Harb. Perspect. Biol. 2013, 5, a011197. [Google Scholar] [CrossRef] [PubMed]
  13. Roberts, A.B.; Lamb, L.C.; Newton, D.L.; Sporn, M.B.; De Larco, J.E.; Todaro, G.J. Transforming growth factors: Isolation of polypeptides from virally and chemically transformed cells by acid/ethanol extraction. Proc. Natl. Acad. Sci. USA 1980, 77, 3494–3498. [Google Scholar] [CrossRef] [PubMed]
  14. Burt, D.W.; Law, A.S. Evolution of the transforming growth factor-β superfamily. Prog. Growth Factor Res. 1994, 5, 99–118. [Google Scholar] [CrossRef]
  15. Akhurst, R.J.; Hata, A. Targeting the TGFβ signalling pathway in disease. Nat. Rev. Drug Discov. 2012, 11, 790–811. [Google Scholar] [CrossRef] [PubMed]
  16. Matzuk, M.M.; Kumar, T.R.; Vassalli, A.; Bickenbach, J.R.; Roop, D.R.; Jaenisch, R.; Bradley, A. Functional analysis of activins during mammalian development. Nature 1995, 374, 354–356. [Google Scholar] [CrossRef] [PubMed]
  17. Zipori, D.; Barda-Saad, M. Role of activin A in negative regulation of normal and tumor B lymphocytes. J. Leukoc. Biol. 2001, 69, 867–873. [Google Scholar] [PubMed]
  18. Robson, N.C.; Phillips, D.J.; McAlpine, T.; Shin, A.; Svobodova, S.; Toy, T.; Pillay, V.; Kirkpatrick, N.; Zanker, D.; Wilson, K.; et al. Activin-A: A novel dendritic cell-derived cytokine that potently attenuates CD40 ligand-specific cytokine and chemokine production. Blood 2008, 111, 2733–2743. [Google Scholar] [CrossRef] [PubMed]
  19. Hedger, M.P.; Drummond, A.E.; Robertson, D.M.; Risbridger, G.P.; de Kretser, D.M. Inhibin and activin regulate [3H]thymidine uptake by rat thymocytes and 3T3 cells in vitro. Mol. Cell. Endocrinol. 1989, 61, 133–138. [Google Scholar] [CrossRef]
  20. Hedger, M.P.; Clarke, L. Isolation of rat blood lymphocytes using a two-step Percoll density gradient. Effect of activin (erythroid differentiation factor) on peripheral T lymphocyte proliferation in vitro. J. Immunol. Methods 1993, 163, 133–136. [Google Scholar] [CrossRef]
  21. Huber, S.; Stahl, F.R.; Schrader, J.; Luth, S.; Presser, K.; Carambia, A.; Flavell, R.A.; Werner, S.; Blessing, M.; Herkel, J.; et al. Activin A Promotes the TGF-β-Induced Conversion of CD4+CD25− T Cells into Foxp3+ Induced Regulatory T Cells. J. Immunol. 2009, 182, 4633–4640. [Google Scholar] [CrossRef] [PubMed]
  22. Sakaguchi, S.; Wing, K.; Onishi, Y.; Prieto-Martin, P.; Yamaguchi, T. Regulatory T cells: How do they suppress immune responses? Int. Immunol. 2009, 21, 1105–1111. [Google Scholar] [CrossRef] [PubMed]
  23. Miyara, M.; Gorochov, G.; Ehrenstein, M.; Musset, L.; Sakaguchi, S.; Amoura, Z. Human FoxP3+ regulatory T cells in systemic autoimmune diseases. Autoimmun. Rev. 2011, 10, 744–755. [Google Scholar] [CrossRef] [PubMed]
  24. Semitekolou, M.; Alissafi, T.; Aggelakopoulou, M.; Kourepini, E.; Kariyawasam, H.H.; Kay, A.B.; Robinson, D.S.; Lloyd, C.M.; Panoutsakopoulou, V.; Xanthou, G. Activin-A induces regulatory T cells that suppress T helper cell immune responses and protect from allergic airway disease. J. Exp. Med. 2009, 206, 1769–1785. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  25. Hardy, C.L.; Rolland, J.M.; O’Hehir, R.E. The immunoregulatory and fibrotic roles of activin A in allergic asthma. Clin. Exp. Allergy 2015, 45, 1510–1522. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  26. Soler Palacios, B.; Estrada-Capetillo, L.; Izquierdo, E.; Criado, G.; Nieto, C.; Municio, C.; González-Alvaro, I.; Sánchez-Mateos, P.; Pablos, J.L.; Corbí, A.L.; et al. Macrophages from the synovium of active rheumatoid arthritis exhibit an activin A-dependent pro-inflammatory profile. J. Pathol. 2015, 235, 515–526. [Google Scholar] [CrossRef] [PubMed]
  27. Ota, F.; Maeshima, A.; Yamashita, S.; Ikeuchi, H.; Kaneko, Y.; Kuroiwa, T.; Hiromura, K.; Ueki, K.; Kojima, I.; Nojima, Y. Activin A induces cell proliferation of fibroblast-like synoviocytes in rheumatoid arthritis. Arthritis Rheum. 2003, 48, 2442–2449. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  28. Wang, R.N.; Green, J.; Wang, Z.; Deng, Y.; Qiao, M.; Peabody, M.; Zhang, Q.; Ye, J.; Yan, Z.; Denduluri, S.; et al. Bone Morphogenetic Protein (BMP) signaling in development and human diseases. Genes Dis. 2014, 1, 87–105. [Google Scholar] [CrossRef] [PubMed]
  29. Eixarch, H.; Calvo-Barreiro, L.; Montalban, X.; Espejo, C. Bone morphogenetic proteins in multiple sclerosis: Role in neuroinflammation. Brain. Behav. Immun. 2018, 68, 1–10. [Google Scholar] [CrossRef] [PubMed]
  30. Pluchino, S.; Zanotti, L.; Brambilla, E.; Rovere-Querini, P.; Capobianco, A.; Alfaro-Cervello, C.; Salani, G.; Cossetti, C.; Borsellino, G.; Battistini, L.; et al. Immune regulatory neural stem/precursor cells protect from central nervous system autoimmunity by restraining dendritic cell function. PLoS ONE 2009, 4, e5959. [Google Scholar] [CrossRef] [PubMed]
  31. Mausner-Fainberg, K.; Urshansky, N.; Regev, K.; Auriel, E.; Karni, A. Elevated and dysregulated bone morphogenic proteins in immune cells of patients with relapsing-remitting multiple sclerosis. J. Neuroimmunol. 2013, 264, 91–99. [Google Scholar] [CrossRef] [PubMed]
  32. Tang, Y.; Ma, X.; Zhang, H.; Gu, Z.; Hou, Y.; Gilkeson, G.S.; Lu, L.; Zeng, X.; Sun, L. Gene expression profile reveals abnormalities of multiple signaling pathways in mesenchymal stem cell derived from patients with systemic lupus erythematosus. Clin. Dev. Immunol. 2012, 2012, 826182. [Google Scholar] [CrossRef] [PubMed]
  33. Bramlage, C.P.; Häupl, T.; Kaps, C.; Ungethüm, U.; Krenn, V.; Pruss, A.; Müller, G.A.; Strutz, F.; Burmester, G.R. Decrease in expression of bone morphogenetic proteins 4 and 5 in synovial tissue of patients with osteoarthritis and rheumatoid arthritis. Arthritis Res. Ther. 2006, 8, R58. [Google Scholar] [CrossRef] [PubMed]
  34. Lories, R.J.; Derese, I.; Ceuppens, J.L.; Luyten, F.P. Bone morphogenetic proteins 2 and 6, expressed in arthritic synovium, are regulated by proinflammatory cytokines and differentially modulate fibroblast-like synoviocyte apoptosis. Arthritis Rheum. 2003, 48, 2807–2818. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  35. Travis, M.A.; Sheppard, D. TGF-β Activation and Function in Immunity. Annu. Rev. Immunol. 2014, 32, 51–82. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  36. Li, M.O.; Wan, Y.Y.; Flavell, R.A. T cell-produced transforming growth factor-β1 controls T cell tolerance and regulates Th1- and Th17-cell differentiation. Immunity 2007, 26, 579–591. [Google Scholar] [CrossRef] [PubMed]
  37. Wan, Y.Y.; Flavell, R.A. “Yin-Yang” functions of transforming growth factor-β and T regulatory cells in immune regulation. Immunol. Rev. 2007, 220, 199–213. [Google Scholar] [CrossRef] [PubMed]
  38. Nakamura, K.; Kitani, A.; Strober, W. Cell contact-dependent immunosuppression by CD4(+)CD25(+) regulatory T cells is mediated by cell surface-bound transforming growth factor β. J. Exp. Med. 2001, 194, 629–644. [Google Scholar] [CrossRef] [PubMed]
  39. Roncarolo, M.G.; Levings, M.K.; Traversari, C. Differentiation of T regulatory cells by immature dendritic cells. J. Exp. Med. 2001, 193, F5-9. [Google Scholar] [CrossRef] [PubMed]
  40. Roncarolo, M.G.; Bacchetta, R.; Bordignon, C.; Narula, S.; Levings, M.K. Type 1 T regulatory cells. Immunol. Rev. 2001, 182, 68–79. [Google Scholar] [CrossRef] [PubMed]
  41. Gutcher, I.; Donkor, M.K.; Ma, Q.; Rudensky, A.Y.; Flavell, R.A.; Li, M.O. Autocrine transforming growth factor-β1 promotes in vivo Th17 cell differentiation. Immunity 2011, 34, 396–408. [Google Scholar] [CrossRef] [PubMed]
  42. Worthington, J.J.; Kelly, A.; Smedley, C.; Bauché, D.; Campbell, S.; Marie, J.C.; Travis, M.A. Integrin αvβ8-Mediated TGF-β Activation by Effector Regulatory T Cells is Essential for Suppression of T-Cell-Mediated Inflammation. Immunity 2015, 42, 903–915. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  43. Dullaers, M.; Li, D.; Xue, Y.; Ni, L.; Gayet, I.; Morita, R.; Ueno, H.; Palucka, K.A.; Banchereau, J.; Oh, S. AT cell-dependent mechanism for the induction of human mucosal homing immunoglobulin A-secreting plasmablasts. Immunity 2009, 30, 120–129. [Google Scholar] [CrossRef] [PubMed]
  44. Sage, P.T.; Sharpe, A.H. T Follicular Regulatory Cells in the Regulation of B cell Responses. Trends Immunol. 2015, 36, 410–418. [Google Scholar] [CrossRef] [PubMed]
  45. Sage, P.T.; Sharpe, A.H. T follicular regulatory cells. Immunol. Rev. 2016, 271, 246–259. [Google Scholar] [CrossRef] [PubMed]
  46. Parekh, V.V.; Prasad, D.V.R.; Banerjee, P.P.; Joshi, B.N.; Kumar, A.; Mishra, G.C. B cells activated by lipopolysaccharide, but not by anti-Ig and anti-CD40 antibody, induce anergy in CD8+ T cells: Role of TGF-β1. J. Immunol. 2003, 170, 5897–5911. [Google Scholar] [CrossRef] [PubMed]
  47. Bjarnadóttir, K.; Benkhoucha, M.; Merkler, D.; Weber, M.S.; Payne, N.L.; Bernard, C.C.; Molnarfi, N.; Lalive, P.H. B cell-derived transforming growth factor-β1 expression limits the induction phase of autoimmune neuroinflammation. Sci. Rep. 2016, 6, 34594. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  48. Molnarfi, N.; Bjarnadóttir, K.; Benkhoucha, M.; Juillard, C.; Lalive, P.H. Activation of human B cells negatively regulates TGF-β1 production. J. Neuroinflamm. 2017, 14, 13. [Google Scholar] [CrossRef] [PubMed]
  49. Gray, J.D.; Hirokawa, M.; Ohtsuka, K.; Horwitz, D.A. Generation of an inhibitory circuit involving CD8+ T cells, IL-2, and NK cell-derived TGF-β: Contrasting effects of anti-CD2 and anti-CD3. J. Immunol. 1998, 160, 2248–2254. [Google Scholar] [PubMed]
  50. Esplugues, E.; Sancho, D.; Vega-Ramos, J.; Martínez, C.; Syrbe, U.; Hamann, A.; Engel, P.; Sánchez-Madrid, F.; Lauzurica, P. Enhanced antitumor immunity in mice deficient in CD69. J. Exp. Med. 2003, 197, 1093–1106. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  51. Wang, X.; Li, F.; Xie, L.; Crane, J.; Zhen, G.; Mishina, Y.; Deng, R.; Gao, B.; Chen, H.; Liu, S.; et al. Inhibition of overactive TGF-β attenuates progression of heterotopic ossification in mice. Nat. Commun. 2018, 9, 551. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  52. Min, K.M.; Kim, P.H. Macrophage-derived TGF-β1 induces IgA isotype expression. Mol. Cells 2003, 16, 245–250. [Google Scholar] [PubMed]
  53. Morelli, A.E.; Zahorchak, A.F.; Larregina, A.T.; Colvin, B.L.; Logar, A.J.; Takayama, T.; Falo, L.D.; Thomson, A.W. Cytokine production by mouse myeloid dendritic cells in relation to differentiation and terminal maturation induced by lipopolysaccharide or CD40 ligation. Blood 2001, 98, 1512–1523. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  54. Lindstedt, K.A.; Wang, Y.; Shiota, N.; Saarinen, J.; Hyytiäinen, M.; Kokkonen, J.O.; Keski-Oja, J.; Kovanen, P.T. Activation of paracrine TGF-β1 signaling upon stimulation and degranulation of rat serosal mast cells: A novel function for chymase. FASEB J. 2001, 15, 1377–1388. [Google Scholar] [CrossRef] [PubMed]
  55. Kobayashi, T.; Iijima, K.; Kita, H. Marked airway eosinophilia prevents development of airway hyper-responsiveness during an allergic response in IL-5 transgenic mice. J. Immunol. 2003, 170, 5756–5763. [Google Scholar] [CrossRef] [PubMed]
  56. Chu, V.T.; Beller, A.; Rausch, S.; Strandmark, J.; Zänker, M.; Arbach, O.; Kruglov, A.; Berek, C. Eosinophils promote generation and maintenance of immunoglobulin-A-expressing plasma cells and contribute to gut immune homeostasis. Immunity 2014, 40, 582–593. [Google Scholar] [CrossRef] [PubMed]
  57. Weller, P.F.; Spencer, L.A. Functions of tissue-resident eosinophils. Nat. Rev. Immunol. 2017, 17, 746–760. [Google Scholar] [CrossRef] [PubMed]
  58. Wang, S.; Xia, P.; Chen, Y.; Qu, Y.; Xiong, Z.; Ye, B.; Du, Y.; Tian, Y.; Yin, Z.; Xu, Z.; et al. Regulatory Innate Lymphoid Cells Control Innate Intestinal Inflammation. Cell 2017, 171, 201–216. [Google Scholar] [CrossRef] [PubMed]
  59. Okamura, T.; Sumitomo, S.; Morita, K.; Iwasaki, Y.; Inoue, M.; Nakachi, S.; Komai, T.; Shoda, H.; Miyazaki, J.; Fujio, K.; et al. TGF-β3-expressing CD4(+)CD25(−)LAG3(+) regulatory T cells control humoral immune responses. Nat. Commun. 2015, 6, 6329. [Google Scholar] [CrossRef] [PubMed]
  60. Morita, K.; Okamura, T.; Inoue, M.; Komai, T.; Teruya, S.; Iwasaki, Y.; Sumitomo, S.; Shoda, H.; Yamamoto, K.; Fujio, K. Egr2 and Egr3 in regulatory T cells cooperatively control systemic autoimmunity through Ltbp3-mediated TGF-β3 production. Proc. Natl. Acad. Sci. USA 2016, 113, E8131–E8140. [Google Scholar] [CrossRef] [PubMed]
  61. Zhu, B.; Symonds, A.L.; Martin, J.E.; Kioussis, D.; Wraith, D.C.; Li, S.; Wang, P. Early growth response gene 2 (Egr-2) controls the self-tolerance of T cells and prevents the development of lupuslike autoimmune disease. J. Exp. Med. 2008, 205, 2295–2307. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  62. Myouzen, K.; Kochi, Y.; Shimane, K.; Fujio, K.; Okamura, T.; Okada, Y.; Suzuki, A.; Atsumi, T.; Ito, S.; Takada, K.; et al. Regulatory polymorphisms in EGR2 are associated with susceptibility to systemic lupus erythematosus. Hum. Mol. Genet. 2010, 19, 2313–2320. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  63. Okamura, T.; Fujio, K.; Shibuya, M.; Sumitomo, S.; Shoda, H.; Sakaguchi, S.; Yamamoto, K. CD4+CD25−LAG3+ regulatory T cells controlled by the transcription factor Egr-2. Proc. Natl. Acad. Sci. USA 2009, 106, 13974–13979. [Google Scholar] [CrossRef] [PubMed]
  64. Okamura, T.; Yamamoto, K.; Fujio, K. Early Growth Response Gene 2-Expressing CD4+LAG3+ Regulatory T Cells: The Therapeutic Potential for Treating Autoimmune Diseases. Front. Immunol. 2018, 9. [Google Scholar] [CrossRef] [PubMed]
  65. Dijke, P.; Arthur, H.M. Extracellular control of TGFβ signalling in vascular development and disease. Nat. Rev. Mol. Cell Biol. 2007, 8, 857–869. [Google Scholar] [CrossRef] [PubMed]
  66. Annes, J.P.; Munger, J.S.; Rifkin, D.B. Making sense of latent TGFβ activation. J. Cell Sci. 2003, 116, 217–224. [Google Scholar] [CrossRef] [PubMed]
  67. Robertson, I.B.; Horiguchi, M.; Zilberberg, L.; Dabovic, B.; Hadjiolova, K.; Rifkin, D.B. Latent TGF-β-binding proteins. Matrix Biol. 2015, 47, 44–53. [Google Scholar] [CrossRef] [PubMed]
  68. Saharinen, J.; Keski-Oja, J. Specific sequence motif of 8-Cys repeats of TGF-β binding proteins, LTBPs, creates a hydrophobic interaction surface for binding of small latent TGF-β. Mol. Biol. Cell 2000, 11, 2691–2704. [Google Scholar] [CrossRef] [PubMed]
  69. Chen, Y.; Ali, T.; Todorovic, V.; O’Leary, J.M.; Kristina Downing, A.; Rifkin, D.B. Amino Acid Requirements for Formation of the TGF-β-Latent TGF-β Binding Protein Complexes. J. Mol. Biol. 2005, 345, 175–186. [Google Scholar] [CrossRef] [PubMed]
  70. Yoshinaga, K.; Obata, H.; Jurukovski, V.; Mazzieri, R.; Chen, Y.; Zilberberg, L.; Huso, D.; Melamed, J.; Prijatelj, P.; Todorovic, V.; et al. Perturbation of transforming growth factor (TGF)-β1 association with latent TGF-β binding protein yields inflammation and tumors. Proc. Natl. Acad. Sci. USA 2008, 105, 18758–18763. [Google Scholar] [CrossRef] [PubMed]
  71. Rifkin, D.B.; Rifkin, W.J.; Zilberberg, L. LTBPs in biology and medicine: LTBP diseases. Matrix Biol. 2017. [Google Scholar] [CrossRef] [PubMed]
  72. Shi, M.; Zhu, J.; Wang, R.; Chen, X.; Mi, L.; Walz, T.; Springer, T.A. Latent TGF-β structure and activation. Nature 2011, 474, 343–349. [Google Scholar] [CrossRef] [PubMed]
  73. Yang, Z.; Mu, Z.; Dabovic, B.; Jurukovski, V.; Yu, D.; Sung, J.; Xiong, X.; Munger, J.S. Absence of integrin-mediated TGFβ1 activation in vivo recapitulates the phenotype of TGFβ1-null mice. J. Cell Biol. 2007, 176, 787–793. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  74. Wang, J.; Dong, X.; Zhao, B.; Li, J.; Lu, C.; Springer, T.A. Atypical interactions of integrin αVβ8with pro-TGF-β1. Proc. Natl. Acad. Sci. USA 2017, 114, E4168–E4174. [Google Scholar] [CrossRef] [PubMed]
  75. Aluwihare, P.; Mu, Z.; Zhao, Z.; Yu, D.; Weinreb, P.H.; Horan, G.S.; Violette, S.M.; Munger, J.S. Mice that lack activity of αvβ6- and αvβ8-integrins reproduce the abnormalities of Tgfb1- and Tgfb3-null mice. J. Cell Sci. 2009, 122, 227–232. [Google Scholar] [CrossRef] [PubMed]
  76. Travis, M.A.; Reizis, B.; Melton, A.C.; Masteller, E.; Tang, Q.; Proctor, J.M.; Wang, Y.; Bernstein, X.; Huang, X.; Reichardt, L.F.; et al. Loss of integrin α(v)β8 on dendritic cells causes autoimmunity and colitis in mice. Nature 2007, 449, 361–365. [Google Scholar] [CrossRef] [PubMed]
  77. Massague, J. TGFβ signalling in context. Nat. Rev. Mol. Cell Biol. 2012, 13, 616–630. [Google Scholar] [CrossRef] [PubMed]
  78. Wrighton, K.H.; Lin, X.; Feng, X.H. Phospho-control of TGF-β superfamily signaling. Cell Res. 2009, 19, 8–20. [Google Scholar] [CrossRef] [PubMed]
  79. Goumans, M.J.; Valdimarsdottir, G.; Itoh, S.; Lebrin, F.; Larsson, J.; Mummery, C.; Karlsson, S.; ten Dijke, P. Activin receptor-like kinase (ALK)1 is an antagonistic mediator of lateral TGFβ/ALK5 signaling. Mol. Cell 2003, 12, 817–828. [Google Scholar] [CrossRef]
  80. Derynck, R.; Zhang, Y.E. Smad-dependent and Smad-independent pathways in TGF-β family signalling. Nature 2003, 425, 577–584. [Google Scholar] [CrossRef] [PubMed]
  81. Curado, F.; Spuul, P.; Egana, I.; Rottiers, P.; Daubon, T.; Veillat, V.; Duhamel, P.; Leclercq, A.; Gontier, E.; Genot, E. ALK5 and ALK1 Play Antagonistic Roles in Transforming Growth Factor β-Induced Podosome Formation in Aortic Endothelial Cells. Mol. Cell. Biol. 2014, 34, 4389–4403. [Google Scholar] [CrossRef] [PubMed]
  82. Goumans, M.J.; Liu, Z.; ten Dijke, P. TGF-β signaling in vascular biology and dysfunction. Cell Res. 2009, 19, 116–127. [Google Scholar] [CrossRef] [PubMed]
  83. Cheifetz, S.; Bellón, T.; Calés, C.; Vera, S.; Bernabeu, C.; Massagué, J.; Letarte, M. Endoglin is a component of the transforming growth factor-β receptor system in human endothelial cells. J. Biol. Chem. 1992, 267, 19027–19030. [Google Scholar] [PubMed]
  84. Piek, E.; Moustakas, A.; Kurisaki, A.; Heldin, C.H.; ten Dijke, P. TGF-(β) type I receptor/ALK-5 and Smad proteins mediate epithelial to mesenchymal transdifferentiation in NMuMG breast epithelial cells. J. Cell Sci. 1999, 112 Pt 24, 4557–4568. [Google Scholar] [PubMed]
  85. Dudas, M.; Nagy, A.; Laping, N.J.; Moustakas, A.; Kaartinen, V. Tgf-β3-induced palatal fusion is mediated by Alk-5/Smad pathway. Dev. Biol. 2004, 266, 96–108. [Google Scholar] [CrossRef] [PubMed]
  86. Lane, J.; Yumoto, K.; Azhar, M.; Ninomiya-Tsuji, J.; Inagaki, M.; Hu, Y.; Deng, C.X.; Kim, J.; Mishina, Y.; Kaartinen, V. Tak1, Smad4 and Trim33 redundantly mediate TGF-β3 signaling during palate development. Dev. Biol. 2015, 398, 231–241. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  87. Tsuchida, Y.; Sumitomo, S.; Ishigaki, K.; Suzuki, A.; Kochi, Y.; Tsuchiya, H.; Ota, M.; Komai, T.; Inoue, M.; Morita, K.; et al. TGF-β3 Inhibits Antibody Production by Human B Cells. PLoS ONE 2017, 12, e0169646. [Google Scholar] [CrossRef] [PubMed]
  88. Bakkebø, M.; Huse, K.; Hilden, V.I.; Smeland, E.B.; Oksvold, M.P. TGF-β-induced growth inhibition in B-cell lymphoma correlates with Smad1/5 signalling and constitutively active p38 MAPK. BMC Immunol. 2010, 11, 57. [Google Scholar] [CrossRef] [PubMed]
  89. Lee, Y.; Awasthi, A.; Yosef, N.; Quintana, F.J.; Xiao, S.; Peters, A.; Wu, C.; Kleinewietfeld, M.; Kunder, S.; Hafler, D.A.; et al. Induction and molecular signature of pathogenic TH17 cells. Nat. Immunol. 2012, 13, 991–999. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  90. David, C.J.; Massagué, J. Contextual determinants of TGFβ action in development, immunity and cancer. Nat. Rev. Mol. Cell Biol. 2018. [Google Scholar] [CrossRef] [PubMed]
  91. Kaartinen, V.; Voncken, J.W.; Shuler, C.; Warburton, D.; Bu, D.; Heisterkamp, N.; Groffen, J. Abnormal lung development and cleft palate in mice lacking TGF-β 3 indicates defects of epithelial-mesenchymal interaction. Nat. Genet. 1995, 11, 415–421. [Google Scholar] [CrossRef] [PubMed]
  92. Proetzel, G.; Pawlowski, S.A.; Wiles, M.V.; Yin, M.; Boivin, G.P.; Howles, P.N.; Ding, J.; Ferguson, M.W.; Doetschman, T. Transforming growth factor-β3 is required for secondary palate fusion. Nat. Genet. 1995, 11, 409–414. [Google Scholar] [CrossRef] [PubMed]
  93. Chikuma, S.; Suita, N.; Okazaki, I.M.; Shibayama, S.; Honjo, T. TRIM28 prevents autoinflammatory T cell development in vivo. Nat. Immunol. 2012, 13, 596–603. [Google Scholar] [CrossRef] [PubMed]
  94. McKams, S.C.; Letterio, J.J.; Kaminski, N.E. Concentration-dependent bifunctional effect of TGF-beta 1 on immunoglobulin production: A role for Smad3 inIgA production in vitro. Int. Immunopharmacol. 2003, 3, 1761–1774. [Google Scholar]
  95. McIntyre, T.M.; Klinman, D.R.; Rothman, P.; Lugo, M.; Dasch, J.R.; Mond, J.J.; Snapper, C.M. Transforming growth factor β1 selectivity stimulates immunoglobulin G2b secretion by lipopolysaccharide-activated murine B cells. J. Exp. Med. 1993, 177, 1031–1037. [Google Scholar] [CrossRef] [PubMed]
  96. Komai, T.; Inoue, M.; Okamura, T.; Morita, K.; Iwasaki, Y.; Sumitomo, S.; Shoda, H.; Yamamoto, K.; Fujio, K. Transforming growth factor-β and interleukin-10 synergistically regulate humoral immunity via modulating metabolic signals. Front. Immunol. 2018, 9, 1364. [Google Scholar] [CrossRef] [PubMed]
  97. Hall, B.E.; Wankhade, U.D.; Konkel, J.E.; Cherukuri, K.; Nagineni, C.N.; Flanders, K.C.; Arany, P.R.; Chen, W.; Rane, S.G.; Kulkarni, A.B. Transforming growth factor-β3 (TGF-β3) knock-in ameliorates inflammation due to TGF-β1 deficiency while promoting glucose tolerance. J. Biol. Chem. 2013, 288, 32074–32092. [Google Scholar] [CrossRef] [PubMed]
  98. Shah, S.; Qiao, L. Resting B cells expand a CD4+CD25+Foxp3+ Treg population via TGF-β3. Eur. J. Immunol. 2008, 38, 2488–2498. [Google Scholar] [CrossRef] [PubMed]
  99. Gorelik, L.; Constant, S.; Flavell, R.A. Mechanism of transforming growth factor β-induced inhibition of T helper type 1 differentiation. J. Exp. Med. 2002, 195, 1499–1505. [Google Scholar] [CrossRef] [PubMed]
  100. Gorelik, L.; Fields, P.E.; Flavell, R.A. Cutting edge: TGF-β inhibits Th type 2 development through inhibition of GATA-3 expression. J. Immunol. 2000, 165, 4773–4777. [Google Scholar] [CrossRef] [PubMed]
  101. Viel, S.; Marçais, A.; Guimaraes, F.S.; Loftus, R.; Rabilloud, J.; Grau, M.; Degouve, S.; Djebali, S.; Sanlaville, A.; Charrier, E.; et al. TGF-β inhibits the activation and functions of NK cells by repressing the mTOR pathway. Sci. Signal. 2016, 9, ra19. [Google Scholar] [CrossRef] [PubMed]
  102. Werner, F.; Jain, M.K.; Feinberg, M.W.; Sibinga, N.E.; Pellacani, A.; Wiesel, P.; Chin, M.T.; Topper, J.N.; Perrella, M.A.; Lee, M.E. Transforming Growth Factor-β1 Inhibition of Macrophage Activation is Mediated via Smad3. J. Biol. Chem. 2000, 275, 36653–36658. [Google Scholar] [CrossRef] [PubMed]
  103. Cazac, B.B.; Roes, J. TGF-β receptor controls B cell responsiveness and induction of IgA in vivo. Immunity 2000, 13, 443–451. [Google Scholar] [CrossRef]
  104. Roes, J.; Choi, B.K.; Cazac, B.B. Redirection of B cell responsiveness by transforming growth factor β receptor. Proc. Natl. Acad. Sci. USA 2003, 100, 7241–7246. [Google Scholar] [CrossRef] [PubMed]
  105. Victora, G.D.; Schwickert, T.A.; Fooksman, D.R.; Kamphorst, A.O.; Meyer-Hermann, M.; Dustin, M.L.; Nussenzweig, M.C. Germinal center dynamics revealed by multiphoton microscopy with a photoactivatable fluorescent reporter. Cell 2010, 143, 592–605. [Google Scholar] [CrossRef] [PubMed]
  106. Kräutler, N.J.; Suan, D.; Butt, D.; Bourne, K.; Hermes, J.R.; Chan, T.D.; Sundling, C.; Kaplan, W.; Schofield, P.; Jackson, J.; et al. Differentiation of germinal center B cells into plasma cells is initiated by high-affinity antigen and completed by Tfh cells. J. Exp. Med. 2017, 214, 1259–1267. [Google Scholar] [CrossRef] [PubMed]
  107. Saxena, A.; Khosraviani, S.; Noel, S.; Mohan, D.; Donner, T.; Hamad, A.R. Interleukin-10 paradox: A potent immunoregulatory cytokine that has been difficult to harness for immunotherapy. Cytokine 2015, 74, 27–34. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  108. Bartee, E.; McFadden, G. Cytokine synergy: An underappreciated contributor to innate anti-viral immunity. Cytokine 2013, 63, 237–240. [Google Scholar] [CrossRef] [PubMed]
  109. Yokogawa, M.; Takaishi, M.; Nakajima, K.; Kamijima, R.; Fujimoto, C.; Kataoka, S.; Terada, Y.; Sano, S. Epicutaneous application of toll-like receptor 7 agonists leads to systemic autoimmunity in wild-type mice: A new model of systemic lupus erythematosus. Arthritis Rheumatol. 2014, 66, 694–706. [Google Scholar] [CrossRef] [PubMed]
  110. Bertoli-Avella, A.M.; Gillis, E.; Morisaki, H.; Verhagen, J.M.A.; de Graaf, B.M.; van de Beek, G.; Gallo, E.; Kruithof, B.P.T.; Venselaar, H.; Myers, L.A.; et al. Mutations in a TGF-β Ligand, TGFB3, Cause Syndromic Aortic Aneurysms and Dissections. J. Am. Coll. Cardiol. 2015, 65, 1324–1336. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  111. Lafyatis, R. Transforming growth factor β—At the centre of systemic sclerosis. Nat. Rev. Rheumatol. 2014, 10, 706–719. [Google Scholar] [CrossRef] [PubMed]
  112. Christmann, R.B.; Sampaio-Barros, P.; Stifano, G.; Borges, C.L.; de Carvalho, C.R.; Kairalla, R.; Parra, E.R.; Spira, A.; Simms, R.; Capellozzi, V.L.; et al. Association of Interferon- and transforming growth factor β-regulated genes and macrophage activation with systemic sclerosis-related progressive lung fibrosis. Arthritis Rheumatol. 2014, 66, 714–725. [Google Scholar] [CrossRef] [PubMed]
  113. Dziadzio, M.; Smith, R.E.; Abraham, D.J.; Black, C.M.; Denton, C.P. Circulating levels of active transforming growth factor β1 are reduced in diffuse cutaneous systemic sclerosis and correlate inversely with the modified Rodnan skin score. Rheumatology 2005, 44, 1518–1524. [Google Scholar] [CrossRef] [PubMed]
  114. Higashiyama, H.; Yoshimoto, D.; Kaise, T.; Matsubara, S.; Fujiwara, M.; Kikkawa, H.; Asano, S.; Kinoshita, M. Inhibition of activin receptor-like kinase 5 attenuates bleomycin-induced pulmonary fibrosis. Exp. Mol. Pathol. 2007, 83, 39–46. [Google Scholar] [CrossRef] [PubMed]
  115. Zhang, Y.; McCormick, L.L.; Gilliam, A.C. Latency-associated peptide prevents skin fibrosis in murine sclerodermatous graft-versus-host disease, a model for human scleroderma. J. Investig. Dermatol. 2003, 121, 713–719. [Google Scholar] [CrossRef] [PubMed]
  116. Denton, C.P.; Merkel, P.A.; Furst, D.E.; Khanna, D.; Emery, P.; Hsu, V.M.; Silliman, N.; Streisand, J.; Powell, J.; Åkesson, A.; et al. Scleroderma Clinical Trials Consortium Recombinant human anti-transforming growth factor β1 antibody therapy in systemic sclerosis: A multicenter, randomized, placebo-controlled phase I/II trial of CAT-192. Arthritis Rheum. 2007, 56, 323–333. [Google Scholar] [CrossRef] [PubMed]
  117. Rice, L.M.; Padilla, C.M.; McLaughlin, S.R.; Mathes, A.; Ziemek, J.; Goummih, S.; Nakerakanti, S.; York, M.; Farina, G.; Whitfield, M.L.; et al. Fresolimumab treatment decreases biomarkers and improves clinical symptoms in systemic sclerosis patients. J. Clin. Investig. 2015, 125, 2795–2807. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  118. Gourh, P.; Remmers, E.F.; Satpathy, A.; Boyden, S.; Morgan, N.D.; Shah, A.A.; Adeyemo, A.; Bentley, A.; Carns, M.A.; Chandrasekharappa, S.C.; et al. Transforming Growth Factor β3 (TGFB3)—A Novel Systemic Sclerosis Susceptibility Locus Involved in Fibrosis and Th17 Cell Development Identified By Genome-Wide Association Study in African Americans from the Genome Research in African American Scleroderma Patients Consortium. Arthritis Rheumatol. 2017, 69 (Suppl. 10). [Google Scholar] [CrossRef]
  119. Saxena, V.; Lienesch, D.W.; Zhou, M.; Bommireddy, R.; Azhar, M.; Doetschman, T.; Singh, R.R. Dual roles of immunoregulatory cytokine TGF-β in the pathogenesis of autoimmunity-mediated organ damage. J. Immunol. 2008, 180, 1903–1912. [Google Scholar] [CrossRef] [PubMed]
  120. Kaplan, J.; Woodworth, L.; Smith, K.; Coco, J.; Vitsky, A.; McPherson, J. Therapeutic benefit of treatment with anti-thymocyte globulin and latent TGF-β1 in the MRL/LPR lupus mouse model. Lupus 2008, 17, 822–831. [Google Scholar] [CrossRef] [PubMed]
  121. Hammad, A.M.; Youssef, H.M.; El-Arman, M.M. Transforming growth factor β 1 in children with systemic lupus erythematosus: A possible relation with clinical presentation of lupus nephritis. Lupus 2006, 15, 608–612. [Google Scholar] [CrossRef] [PubMed]
  122. Becker-Merok, A.; Eilertsen, G.Ø.; Nossent, J.C. Levels of transforming growth factor-β are low in systemic lupus erythematosus patients with active disease. J. Rheumatol. 2010, 37, 2039–2045. [Google Scholar] [CrossRef] [PubMed]
  123. Elbeldi-Ferchiou, A.; Ben Ahmed, M.; Smiti-Khanfir, M.; Houman, M.H.; Abdeladhim, M.; Belhadj Hmida, N.; Cerf-Bensussan, N.; Louzir, H. Resistance to exogenous TGF-β effects in patients with systemic lupus erythematosus. J. Clin. Immunol. 2011, 31, 574–583. [Google Scholar] [CrossRef] [PubMed]
  124. Severin, M.E.; Lee, P.W.; Liu, Y.; Selhorst, A.J.; Gormley, M.G.; Pei, W.; Yang, Y.; Guerau-de-Arellano, M.; Racke, M.K.; Lovett-Racke, A.E. MicroRNAs targeting TGFβ signalling underlie the regulatory T cell defect in multiple sclerosis. Brain 2016, 139, 1747–1761. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  125. Calabresi, P.A.; Fields, N.S.; Maloni, H.W.; Hanham, A.; Carlino, J.; Moore, J.; Levin, M.C.; Dhib-Jalbut, S.; Tranquill, L.R.; Austin, H.; et al. Phase 1 trial of transforming growth factor β2 in chronic progressive MS. Neurology 1998, 51, 289–292. [Google Scholar] [CrossRef] [PubMed]
  126. Lee, P.W.; Severin, M.E.; Lovett-Racke, A.E. TGF-β regulation of encephalitogenic and regulatory T cells in multiple sclerosis. Eur. J. Immunol. 2017, 47, 446–453. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  127. Aschner, Y.; Downey, G.P. Transforming Growth Factor-β: Master Regulator of the Respiratory System in Health and Disease. Am. J. Respir. Cell Mol. Biol. 2016, 54, 647–655. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  128. Walton, K.L.; Johnson, K.E.; Harrison, C.A. Targeting TGF-β Mediated SMAD Signaling for the Prevention of Fibrosis. Front. Pharmacol. 2017, 8, 461. [Google Scholar] [CrossRef] [PubMed]
  129. Ferguson, M.W.; Duncan, J.; Bond, J.; Bush, J.; Durani, P.; So, K.; Taylor, L.; Chantrey, J.; Mason, T.; James, G.; et al. Prophylactic administration of avotermin for improvement of skin scarring: Three double-blind, placebo-controlled, phase I/II studies. Lancet 2009, 373, 1264–1274. [Google Scholar] [CrossRef]
  130. De Gramont, A.; Faivre, S.; Raymond, E. Novel TGF-β inhibitors ready for prime time in onco-immunology. Oncoimmunology 2017, 6, e1257453. [Google Scholar] [CrossRef] [PubMed]
  131. Harjes, U. Immunotherapy: Tear down this wall. Nat. Rev. Immunol. 2018, 18, 221. [Google Scholar] [CrossRef] [PubMed]
Ijms 19 02261 g001 550
Figure 1. The roles of Egr in TGF-β3 production in CD4+CD25−LAG3+ regulatory T cells. Synthesized latency-associated peptide (LAP) and mature TGF-β3 binds to latent TGF-β binding protein-3 (LTBP-3), and the large latent complex is secreted from cells. Early growth response gene 2 (Egr2) and Egr3 regulate the expression of Ltbp3, which leads to efficient secretion of TGF-β3 from CD4+CD25−LAG3+ regulatory T cells (LAG3+ Tregs).
Figure 1. The roles of Egr in TGF-β3 production in CD4+CD25−LAG3+ regulatory T cells. Synthesized latency-associated peptide (LAP) and mature TGF-β3 binds to latent TGF-β binding protein-3 (LTBP-3), and the large latent complex is secreted from cells. Early growth response gene 2 (Egr2) and Egr3 regulate the expression of Ltbp3, which leads to efficient secretion of TGF-β3 from CD4+CD25−LAG3+ regulatory T cells (LAG3+ Tregs).
Ijms 19 02261 g001
Ijms 19 02261 g002 550
Figure 2. Schematic description of the “inhibitory cytokine synergy” of TGF-β3 and IL-10 in activated B cells. Phosphorylation of S6 kinase 1 (S6K1) and expression of hypoxia inducible factor 1α (HIF1α), which is a downstream signaling of mammalian target of rapamycin complex 1 (mTORC1), are suppressed by a combination of the inhibitory cytokines TGF-β3 and IL-10. ICS further suppresses B cell cellular energetic metabolism, such as glycolysis and oxidative phosphorylation. Arrows, positive regulation; T-bar, negative regulation.
Figure 2. Schematic description of the “inhibitory cytokine synergy” of TGF-β3 and IL-10 in activated B cells. Phosphorylation of S6 kinase 1 (S6K1) and expression of hypoxia inducible factor 1α (HIF1α), which is a downstream signaling of mammalian target of rapamycin complex 1 (mTORC1), are suppressed by a combination of the inhibitory cytokines TGF-β3 and IL-10. ICS further suppresses B cell cellular energetic metabolism, such as glycolysis and oxidative phosphorylation. Arrows, positive regulation; T-bar, negative regulation.
Ijms 19 02261 g002

Share and Cite

MDPI and ACS Style

Komai, T.; Okamura, T.; Inoue, M.; Yamamoto, K.; Fujio, K. Reevaluation of Pluripotent Cytokine TGF-β3 in Immunity. Int. J. Mol. Sci. 2018, 19, 2261. https://doi.org/10.3390/ijms19082261

AMA Style

Komai T, Okamura T, Inoue M, Yamamoto K, Fujio K. Reevaluation of Pluripotent Cytokine TGF-β3 in Immunity. International Journal of Molecular Sciences. 2018; 19(8):2261. https://doi.org/10.3390/ijms19082261

Chicago/Turabian Style

Komai, Toshihiko, Tomohisa Okamura, Mariko Inoue, Kazuhiko Yamamoto, and Keishi Fujio. 2018. "Reevaluation of Pluripotent Cytokine TGF-β3 in Immunity" International Journal of Molecular Sciences 19, no. 8: 2261. https://doi.org/10.3390/ijms19082261

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop