Age-Related Changes in MicroRNA in the Rat Pituitary and Potential Role in GH Regulation
Abstract
:1. Introduction
2. Results
2.1. Rat Growth Curve, Serum IGF-1 Concentration, and Pituitary GH1, Growth Hormone-Releasing Hormone Receptor(GHRHR), and Somatostatin Receptor 2 (SSTR2) mRNA Expression Assay
2.2. Dynamic Expression of miRNAs in the Pituitary
2.3. Target and Pathway Analysis of Differentially Expressed miRNAs
2.4. Validation of the Pituitary miRNAs via qRT-PCR
2.5. Determination of the Functional miRNA Target Pairs
2.6. miR-141-3p Negatively Regulates Rat Pituitary GH Expression
3. Discussion
4. Materials and Methods
4.1. Animals and Sample Preparation
4.2. Serum IGF-1 Concentration Assay
4.3. RNA Extraction and Quantitative Real-Time PCR Analysis
4.4. MicroRNA Microarray Assay
4.5. Tissue-Specific miRNA Target Prediction and Exploration of the Involved Pathways
4.6. Cell Lines and Cell Transfection
4.7. Western Blot Analysis
4.8. Luciferase Reporter Assay
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
GH | Growth hormone |
miRNA | MicroRNA |
mRNA | Messenger RNA |
RT-PCR | Reverse transcriptase quantitative polymerase chain reaction |
3′UTR | 3′ Untranslated region |
GHRHR | Growth hormone-releasing hormone receptor |
IPA | Ingenuity pathway analysis |
SSTR2 | Somatostatin receptor 2 |
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Pathway | Count of miRNAs |
---|---|
Calcium Signaling | 15 |
Cyclic adenosine monophosphate (cAMP)-mediated Signaling | 15 |
Externally regulated kinases/ mitogen-activated protein kinase (ERK/MAPK) Signaling | 15 |
Glucocorticoid Receptor Signaling | 15 |
Gonadotropin-releasing hormone (GNRH) Signaling | 15 |
Growth Hormone Signaling | 15 |
Insulin-like growth factor 1 (IGF-1) Signaling | 15 |
Insulin Receptor Signaling | 15 |
Protein Kinase A Signaling | 15 |
Retinoic acid receptor (RAR) Activation | 15 |
Wnt/β-catenin Signaling | 15 |
Corticotropin Releasing Hormone Signaling | 14 |
cAMP-response element binding protein (CREB) Signaling in Neurons | 14 |
G-Protein Coupled Receptor Signaling | 14 |
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Zhang, H.; Qi, Q.; Chen, T.; Luo, J.; Xi, Q.; Jiang, Q.; Sun, J.; Zhang, Y. Age-Related Changes in MicroRNA in the Rat Pituitary and Potential Role in GH Regulation. Int. J. Mol. Sci. 2018, 19, 2058. https://doi.org/10.3390/ijms19072058
Zhang H, Qi Q, Chen T, Luo J, Xi Q, Jiang Q, Sun J, Zhang Y. Age-Related Changes in MicroRNA in the Rat Pituitary and Potential Role in GH Regulation. International Journal of Molecular Sciences. 2018; 19(7):2058. https://doi.org/10.3390/ijms19072058
Chicago/Turabian StyleZhang, Haojie, Qien Qi, Ting Chen, Junyi Luo, Qianyun Xi, Qingyan Jiang, Jiajie Sun, and Yongliang Zhang. 2018. "Age-Related Changes in MicroRNA in the Rat Pituitary and Potential Role in GH Regulation" International Journal of Molecular Sciences 19, no. 7: 2058. https://doi.org/10.3390/ijms19072058