Enhancing Chlorobenzene Biodegradation by Delftia tsuruhatensis Using a Water-Silicone Oil Biphasic System
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Microorganism and Culture Medium
2.3. Partition Coefficient Test
2.4. Batch Biodegradation Experiments
2.5. Electron Transport System Activity and Cell Surface Hydrophobicity Tests
2.6. Extracellular Polymeric Substance Analysis
2.7. Staining and Fluorescence Microscopy Observation
2.8. Analytical Methods
3. Results
3.1. Affinity of Silicone Oil for CB
3.2. Performance of the Water–Silicone Oil System
3.3. ETS Activity Assays
3.4. Microbial Distribution in the Water–Silicone Oil System
3.5. Effect of Silicone Oil on the Surface Characteristics of D. tsuruhatensis LW26
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Initial Amount of CB in Serum Bottle (mg) | ETS Activity (μmol-INTF/(g Cell Dry Weight·h)) | |
---|---|---|
Single-phase system | Biphasic system | |
11 | 384.2 ± 51 | 396.4 ± 25 |
22 | 182.7 ± 25 | 283.5 ± 32 |
33 | -- | 234.9 ± 27 |
System | CSH (%) | Protein in EPS (mg/g) | Polysaccharide in EPS (mg/g) | EPS (mg/g) | PN a/PS b |
---|---|---|---|---|---|
Single-phase system | 5.3 ± 0.4 | 18.2 ± 3.5 | 12.3 ± 3.1 | 30.5 ± 6.6 | 1.5 |
Biphasic system | 42.5 ± 5.4 | 34.2 ± 5.8 | 7.5 ± 2.2 | 41.7 ± 8 | 4.6 |
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Ye, J.-X.; Lin, T.-H.; Hu, J.-T.; Poudel, R.; Cheng, Z.-W.; Zhang, S.-H.; Chen, J.-M.; Chen, D.-Z. Enhancing Chlorobenzene Biodegradation by Delftia tsuruhatensis Using a Water-Silicone Oil Biphasic System. Int. J. Environ. Res. Public Health 2019, 16, 1629. https://doi.org/10.3390/ijerph16091629
Ye J-X, Lin T-H, Hu J-T, Poudel R, Cheng Z-W, Zhang S-H, Chen J-M, Chen D-Z. Enhancing Chlorobenzene Biodegradation by Delftia tsuruhatensis Using a Water-Silicone Oil Biphasic System. International Journal of Environmental Research and Public Health. 2019; 16(9):1629. https://doi.org/10.3390/ijerph16091629
Chicago/Turabian StyleYe, Jie-Xu, Tong-Hui Lin, Jing-Tao Hu, Rabin Poudel, Zhuo-Wei Cheng, Shi-Han Zhang, Jian-Meng Chen, and Dong-Zhi Chen. 2019. "Enhancing Chlorobenzene Biodegradation by Delftia tsuruhatensis Using a Water-Silicone Oil Biphasic System" International Journal of Environmental Research and Public Health 16, no. 9: 1629. https://doi.org/10.3390/ijerph16091629