Nondestructive Evaluation of Wet Aged Beef by Novel Electrical Indexes: A Preliminary Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Aging Treatment
2.2. Measurement of Electrical Properties
2.3. Electrical Indexes
2.3.1. Impedance Ratio (IR)
2.3.2. Integrated Impedance Ratio (IIR)
2.3.3. Normalized Impedance Ratio (NIR)
2.3.4. Modified AlY (Mod AlY)
2.4. Determination of Meat Quality Parameters
2.4.1. Determination of Drip Loss
2.4.2. Water-Holding Capacity (WHC)
2.4.3. Texture and Cooking Loss
2.5. Histological Analysis
2.6. Statistical Analyses
3. Results and Discussion
3.1. Drip Loss, Cooking Loss, WHC, and Shear Force
3.2. Electrical Parameters
3.2.1. Impedance |Z| Value
3.2.2. Impedance Ratio (IR)
3.2.3. Electrical Indexes
3.3. Correlations between Electrical Indexes, Physical Properties, and Aging Days
3.4. Histological Analysis
4. Conclusions
- (1)
- Measurement of the changes in the electrical indexes of aging beef by using several types of electrodes and many samples in order to determine a more reliable nondestructive measuring method.
- (2)
- Quantification of the degradation of muscle fiber during aging by using methods such as SDS-PAGE and Western blotting. Analysis with these methods may help to elucidate the mechanism of changes in drip loss and WHC during the aging period as well as to clarify the relationship between physical properties and electrical indexes.
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Parameter | Aging Period (Days) | p-Value | |||
---|---|---|---|---|---|
0 | 2 | 7 | 21 | ||
Drip loss (%) | 0.6 ± 0.16 a,b | 0.3 ± 0.10 a | 0.3 ± 0.10 a | 0.9 ± 0.10 b | <0.01 |
Cooking loss (%) | 18.8 ± 1.85 | 19.5 ± 2.01 | 19.8 ± 0.88 | 21.0 ± 0.80 | >0.05 |
WHC (%) | 71.8 ± 2.67 b | 83.0 ± 3.93 a | 78.4 ± 2.48 a | 80.0 ± 3.20 a | <0.05 |
Shear force (N) | 35.5 ± 6.45 a | 24.7 ± 5.87 a,b | 17.4 ± 8.17 b | 18.3 ± 4.19 b | <0.01 |
Electrical Index | Aging Period (Days) | ||||
---|---|---|---|---|---|
0 | 2 | 7 | 21 | ||
IIR (×104 Hz) | Plate * | 1.69 ± 0.35 | 1.39 ± 0.26 | 1.37 ± 0.26 | 1.10 ± 0.04 |
Needle * | 1.85 ± 0.21 a | 1.35 ± 0.16 b | 1.24 ± 0.14 b | 1.15 ± 0.16 b | |
NIR | Plate * | 1.63 ± 0.39 | 1.30 ± 0.24 | 1.26 ± 0.24 | 1.03 ± 0.04 |
Needle * | 1.79 ± 0.24 a | 1.26 ± 0.15 b | 1.16 ± 0.13 b | 1.08 ± 0.14 b | |
Mod AlY | Plate | 68.7 ± 25.05 | 47.8 ± 25.17 | 35.7 ± 23.61 | 33.6 ± 7.79 |
Needle | 65.1 ± 22.91 | 50.7 ± 23.26 | 45.0 ± 12.51 | 31.4 ± 9.01 |
Electrical Index | Aging Days | Shear Force A | Drip Loss | Cooking Loss | WHC | |
---|---|---|---|---|---|---|
IIR | Plate | −0.616 * | 0.760 ** | −0.424 | −0.567 * | −0.527 * |
Needle | −0.626 * | 0.751 ** | −0.061 | −0.478 | −0.636 * | |
NIR | Plate | −0.585 * | 0.722 * | −0.401 | −0.594 * | −0.530 * |
Needle | −0.616 * | 0.754 ** | −0.051 | −0.509 * | −0.660 * | |
Mod AlY | Plate | −0.591 * | 0.748 ** | −0.355 | −0.599 * | −0.565 * |
Needle | −0.605 * | 0.750 ** | −0.050 | −0.529 * | −0.667 * |
Electrical Index | Type of Electrode | R2 Value |
---|---|---|
IIR (×104 Hz) | Plate | 0.58 |
Needle | 0.56 | |
NIR | Plate | 0.52 |
Needle | 0.52 | |
Mod AlY | Plate | 0.56 |
Needle | 0.56 |
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Ihara, S.; Islam, M.Z.; Kitamura, Y.; Kokawa, M.; Lee, Y.-C.; Chen, S. Nondestructive Evaluation of Wet Aged Beef by Novel Electrical Indexes: A Preliminary Study. Foods 2019, 8, 313. https://doi.org/10.3390/foods8080313
Ihara S, Islam MZ, Kitamura Y, Kokawa M, Lee Y-C, Chen S. Nondestructive Evaluation of Wet Aged Beef by Novel Electrical Indexes: A Preliminary Study. Foods. 2019; 8(8):313. https://doi.org/10.3390/foods8080313
Chicago/Turabian StyleIhara, Shinobu, Md Zohurul Islam, Yutaka Kitamura, Mito Kokawa, Yeun-Chung Lee, and Suming Chen. 2019. "Nondestructive Evaluation of Wet Aged Beef by Novel Electrical Indexes: A Preliminary Study" Foods 8, no. 8: 313. https://doi.org/10.3390/foods8080313